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Understanding tendon mechanical properties, such as stiffness and hysteresis, can provide insights into injury mechanisms. This research addresses the inconsistency of previously reported in-vivo and in-vitro tendon hysteresis properties. Although limited, our preliminary findings suggest that in-vivo hystereses (Mean ± SD; 55% ± 9%) are greater than in-vitro hystereses (14% ± 1%) when directly comparing the same tendon for the same loading conditions in a sheep model in-vivo versus within 24 h post-mortem. Overall, it therefore appears that the tendon mechanical properties are affected by the testing environment, possibly related to differences in muscle-tendon interactions and fluid flow experienced in-vivo versus in-vitro. This communication advocates for more detailed investigations into the mechanisms resulting in the reported differences in tendon behaviour. Overall, such knowledge contributes to our understanding of tendon function towards improving modelling and clinical interventions, bridging the gap between in-vivo and in-vitro observations and enhancing the translational relevance of biomechanical studies.
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PURPOSE: In this study, we compared the influence of movement specificity during velocity-load jump testing to predict on-ice acceleration performance in elite speed skaters. METHODS: Elite long-track speed skaters (N = 27) performed velocity-load testing with 3 external loads during unilateral horizontal jumping, lateral jumping, and bilateral vertical countermovement jumping. For the unilateral tests, external load conditions were set to 10 N, 7.5% and 15% of external load relative to body weight. For the countermovement jumping, load conditions were body weight and 30% and 60% of external load relative to body weight. On-ice performance measures were obtained during maximal 50-m accelerations from a standing start, including maximal skating speed, maximal acceleration capacity, and maximum horizontal power. The 100-m split time from a 500-m race was also obtained. Regularized regression models were used to identify the most important predictors of on-ice acceleration performance. In addition to regularized regression coefficients, Pearson correlation coefficients (r) were calculated for all variables retained by the model to assess interrelationships between single predictors and on-ice performance measures. RESULTS: The countermovement jump with 30% of body mass demonstrated the strongest association with maximal skating speed, maximum horizontal power, and 100-m time (regularized regression coefficient = .16-.49, r = .84-.97, P < .001). Horizontal jump with 15% of body mass was the strongest predictor of maximal acceleration capacity performance (regularized regression coefficient = .08, r = .83, P < .001). CONCLUSIONS: The findings of this study suggest that mechanical specificity rather than movement specificity was more relevant for predicting on-ice acceleration performance.
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Aceleración , Rendimiento Atlético , Patinación , Humanos , Patinación/fisiología , Rendimiento Atlético/fisiología , Masculino , Adulto Joven , Movimiento/fisiología , Prueba de Esfuerzo/métodos , Adulto , FemeninoRESUMEN
Cell volume and shape changes play a pivotal role in cellular mechanotransduction, governing cellular responses to external loading. Understanding the dynamics of cell behavior under loading conditions is essential to elucidate cell adaptation mechanisms in physiological and pathological contexts. In this study, we investigated the effects of dynamic cyclic compression loading on cell volume and shape changes, comparing them with static conditions. Using a custom-designed platform which allowed for simultaneous loading and imaging of cartilage tissue, tissues were subjected to 100 cycles of mechanical loading while measuring cell volume and shape alterations during the unloading phase at specific time points. The findings revealed a transient decrease in cell volume (13%) during the early cycles, followed by a gradual recovery to baseline levels after approximately 20 cycles, despite the cartilage tissue not being fully recovered at the unloading phase. This observed pattern indicates a temporal cell volume response that may be associated with cellular adaptation to the mechanical stimulus through mechanisms related to active cell volume regulation. Additionally, this study demonstrated that cell volume and shape responses during dynamic loading were significantly distinct from those observed under static conditions. Such findings suggest that cells in their natural tissue environment perceive and respond differently to dynamic compared to static mechanical cues, highlighting the significance of considering dynamic loading environments in studies related to cellular mechanics. Overall, this research contributes to the broader understanding of cellular behavior under mechanical stimuli, providing valuable insights into their ability to adapt to dynamic mechanical loading.
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Condrocitos , Soporte de Peso , Animales , Condrocitos/fisiología , Soporte de Peso/fisiología , Estrés Mecánico , Tamaño de la Célula , Mecanotransducción Celular/fisiología , Fuerza Compresiva/fisiología , Bovinos , Cartílago Articular/fisiología , Forma de la Célula/fisiologíaRESUMEN
ABSTRACT: McClean, ZJ, Pasanen, K, Lun, V, Charest, J, Herzog, W, Werthner, P, Black, A, Vleuten, RV, Lacoste, E, and Jordan, MJ. A biopsychosocial model for understanding training load, fatigue, and musculoskeletal sport injury in university athletes: A scoping review. J Strength Cond Res 38(6): 1177-1188, 2024-The impact of musculoskeletal (MSK) injury on athlete health and performance has been studied extensively in youth sport and elite sport. Current research examining the relationship between training load, injury, and fatigue in university athletes is sparse. Furthermore, a range of contextual factors that influence the training load-fatigue-injury relationship exist, necessitating an integrative biopsychosocial model to address primary and secondary injury prevention research. The objectives of this review were (a) to review the scientific literature examining the relationship between training load, fatigue, and MSK injury in university athletes and (b) to use this review in conjunction with a transdisciplinary research team to identify biopsychosocial factors that influence MSK injury and develop an updated, holistic biopsychosocial model to inform injury prevention research and practice in university sport. Ten articles were identified for inclusion in this review. Key findings were an absence of injury surveillance methodology and contextual factors that can influence the training load-fatigue-MSK injury relationship. We highlight the inclusion of academic load, social load, and mental health load as key variables contributing to a multifactorial, gendered environmental, scientific inquiry on sport injury and reinjury in university sport. An integrative biopsychosocial model for MSK injury in university sport is presented that can be used to study the biological, psychological, and social factors that modulate injury and reinjury risk in university athletes. Finally, we provide an example of how causal inference can be used to maximize the utility of longitudinally collected observational data that is characteristic of sport performance research in university sport.
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Atletas , Traumatismos en Atletas , Modelos Biopsicosociales , Humanos , Traumatismos en Atletas/psicología , Universidades , Atletas/psicología , Acondicionamiento Físico Humano/fisiología , Acondicionamiento Físico Humano/psicología , Fatiga/psicología , Sistema Musculoesquelético/lesionesRESUMEN
Little is known regarding the precise muscle, bone and joint actions resulting from individual and simultaneous muscle activation(s) of the lower limb. An in situ experimental approach is described herein to control the muscles of the rabbit lower hindlimb, including the medial and lateral gastrocnemius, soleus, plantaris and tibialis anterior. The muscles were stimulated using nerve-cuff electrodes placed around the innervating nerves of each muscle. Animals were fixed in a stereotactic frame with the ankle angle set at 90 deg. To demonstrate the efficacy of the experimental technique, isometric plantarflexion torque was measured at the 90 deg ankle joint angle at a stimulation frequency of 100, 60 and 30â Hz. Individual muscle torque and the torque produced during simultaneous activation of all plantarflexor muscles are presented for four animals. These results demonstrate that the experimental approach was reliable, with insignificant variation in torque between repeated contractions. The experimental approach described herein provides the potential for measuring a diverse array of muscle properties, which is important to improve our understanding of musculoskeletal biomechanics.
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Miembro Posterior , Músculo Esquelético , Torque , Animales , Conejos , Músculo Esquelético/fisiología , Músculo Esquelético/inervación , Miembro Posterior/fisiología , Fenómenos Biomecánicos , Estimulación Eléctrica , MasculinoRESUMEN
Skeletal muscles and the tendons that attach them to bone are structurally complex and deform non-uniformly during contraction. While these tissue deformations dictate force production during movement, our understanding of this behaviour is limited due to challenges in obtaining complete measures of the constituent structures. To address these challenges, we present an approach for simultaneously measuring muscle, fascicle, aponeurosis, and tendon behaviour using sonomicrometry. To evaluate this methodology, we conducted isometric and dynamic contractions in in situ rabbit medial gastrocnemius. We found comparable patterns of strain in the muscle belly, fascicle, aponeurosis, and tendon during the isometric trials to those published in the literature. For the dynamic contractions, we found that our measures using this method were consistent across all animals and aligned well with our theoretical understanding of muscle-tendon unit behaviour. Thus, this method provides a means to fully capture the complex behaviour of muscle-tendon units across contraction types.
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Aponeurosis , Contracción Muscular , Músculo Esquelético , Tendones , Tendones/fisiología , Animales , Conejos , Aponeurosis/fisiología , Músculo Esquelético/fisiología , Contracción Muscular/fisiología , Fenómenos BiomecánicosRESUMEN
Osteoarthritis (OA) is a multifactorial joint disease characterized by articular cartilage degradation. Risk factors for OA include joint trauma, obesity, and inflammation, each of which can affect joint health independently, but their interaction and the associated consequences of such interaction were largely unexplored. Here, we studied compositional and structural alterations in knee joint cartilages of Sprague-Dawley rats exposed to two OA risk factors: joint injury and diet-induced obesity. Joint injury was imposed by surgical transection of anterior cruciate ligaments (ACLx), and obesity was induced by a high fat/high sucrose diet. Depth-dependent proteoglycan (PG) content and collagen structural network of cartilage were measured from histological sections collected previously in Collins et al.. (2015). We found that ACLx primarily affected the superficial cartilages. Compositionally, ACLx led to reduced PG content in lean animals, but increased PG content in obese rats. Structurally, ACLx caused disorganization of collagenous network in both lean and obese animals through increased collagen orientation in the superficial tissues and a change in the degree of fibrous alignment. However, the cartilage degradation attributed to joint injury and obesity was not necessarily additive when the two risk factors were present simultaneously, particularly for PG content and collagen orientation in the superficial tissues. Interestingly, sham surgeries caused a through-thickness disorganization of collagen network in lean and obese animals. We conclude that the interactions of multiple OA risk factors are complex and their combined effects cannot be understood by superposition principle. Further research is required to elucidate the interactive mechanism between OA subtypes.
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Cartílago Articular , Osteoartritis , Ratas , Animales , Ratas Sprague-Dawley , Articulación de la Rodilla/patología , Osteoartritis/patología , Proteoglicanos/metabolismo , Obesidad/metabolismo , Cartílago Articular/patología , Colágeno/metabolismoRESUMEN
PURPOSE: This study compares the acute effects of a static stretching and a warmup protocol on the active and passive shear modulus of the hamstring muscles. METHODS: Muscle shear modulus was assessed at rest and during isometric contractions at 20â¯% of maximal voluntary isometric contraction (MVIC). RESULTS: After stretching, the passive shear modulus pattern was not altered, while at 20â¯% MVIC the biceps femoris short head (BFsh) and semimembranosus showed a shear modulus increase and decrease, respectively, which resulted on BFsh-SM pair differences (pre: 3.8⯱â¯16.8 vs. post: 39.3⯱â¯25.1â¯kPa; pâ¯<â¯0.001; dâ¯=â¯1.66) which was accompanied by a decrease of 18.3â¯% on MVIC. Following the warmup protocol, passive shear modulus remained unchanged, while active shear modulus was decreased for the semitendinosus (pre: 65.3⯱â¯13.5 vs. post: 60.3⯱â¯12.3â¯kPa; pâ¯=â¯0.035; dâ¯=â¯0.4). However, this difference was within the standard error of measurement (10.54â¯kPa), and did not impact the force production, since it increased only 1.4â¯% after the warmup. CONCLUSIONS: The results of this study suggest that the passive and active shear modulus responses of the individual hamstring muscles to static stretching are muscle-specific and that passive and active hamstring shear modulus are not changed by a standard warmup intervention.
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Músculos Isquiosurales , Contracción Isométrica , Ejercicios de Estiramiento Muscular , Ejercicio de Calentamiento , Humanos , Músculos Isquiosurales/fisiología , Ejercicios de Estiramiento Muscular/fisiología , Contracción Isométrica/fisiología , Masculino , Adulto Joven , Ejercicio de Calentamiento/fisiología , Adulto , Módulo de Elasticidad , UltrasonografíaRESUMEN
This study aimed to determine whether changes in hamstrings passive and active shear modulus after a warmup protocol are correlated. Twenty males without a history of hamstring strain injury participated. Muscle shear modulus was assessed using ultrasound-based shear wave elastography at rest and during isometric contractions at 20% of maximal voluntary isometric effort before and immediately after a warmup protocol. Changes in passive shear modulus did not seem to be associated with changes in active shear modulus. The results of this study suggest that changes in passive and active hamstring shear modulus are not associated after a standardized warmup intervention.
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Diagnóstico por Imagen de Elasticidad , Músculos Isquiosurales , Masculino , Humanos , Músculo Esquelético/diagnóstico por imagen , Músculo Esquelético/fisiología , Módulo de Elasticidad/fisiología , Músculos Isquiosurales/diagnóstico por imagen , Músculos Isquiosurales/fisiología , Diagnóstico por Imagen de Elasticidad/métodos , UltrasonografíaRESUMEN
Perfect mechanical force effectiveness in cycling would be achieved if the forces applied to the pedal were perpendicular to the crank throughout the full crank cycle. However, empirical observations show that resultant pedal forces display substantial radial components in recreational and even highly-trained elite cyclists. Therefore, we hypothesized that attempting to maximize mechanical effectiveness during the entire downstroke of the pedal cycle must be associated with a penalty that outweighs the benefits of perfect effectiveness. Twenty recreational cyclists performed maximum isometric voluntary contractions at five static crank positions in the downstroke phase of cycling for two testing conditions: (i) a non-constrained (NC) condition, where athletes were asked to produce the maximum force possible on the pedal without consideration of the force direction and (ii) a constrained (C) condition, with the instruction to produce maximal pedal forces perpendicular to the crank. Resultant force and effective force (force perpendicular to the crank in the NC conditions) were compared to the force in the C condition that was, by definition, perpendicular to the crank. Maximum effective force in the NC condition was greater (mean = 50 %, range = 38-69 %) than for the C condition across all crank positions. Applying forces perpendicular to the crank in the downstroke of the pedal cycle resulted in severe reductions in force magnitude, suggesting that coaches and athletes should not attempt to change cycling technique towards perfect force effectiveness.
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Ciclismo , Pie , Humanos , Atletas , Gravitación , Fenómenos BiomecánicosRESUMEN
Multiscale models aiming to connect muscle's molecular and cellular function have been difficult to develop, in part due to a lack of self-consistent multiscale data. To address this gap, we measured the force response from single, skinned rabbit psoas muscle fibers to ramp shortenings and step stretches performed on the plateau region of the force-length relationship. We isolated myosin from the same muscles and, under similar conditions, performed single-molecule and ensemble measurements of myosin's ATP-dependent interaction with actin using laser trapping and in vitro motility assays. We fit the fiber data by developing a partial differential equation model that includes thick filament activation, whereby an increase in force on the thick filament pulls myosin out of an inhibited state. The model also includes a series elastic element and a parallel elastic element. This parallel elastic element models a titin-actin interaction proposed to account for the increase in isometric force after stretch (residual force enhancement). By optimizing the model fit to a subset of our fiber measurements, we specified seven unknown parameters. The model then successfully predicted the remainder of our fiber measurements and also our molecular measurements from the laser trap and in vitro motility. The success of the model suggests that our multiscale data are self-consistent and can serve as a testbed for other multiscale models. Moreover, the model captures the decrease in isometric force observed in our muscle fibers after active shortening (force depression), suggesting a molecular mechanism for force depression, whereby a parallel elastic element combines with thick filament activation to decrease the number of cycling cross-bridges.
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Actinas , Depresión , Animales , Conejos , Sarcómeros/fisiología , Fibras Musculares Esqueléticas/fisiología , Miosinas , Contracción MuscularRESUMEN
Many arthroscopic tools developed for knee joint assessment are contact-based, which is challenging for in vivo application in narrow joint spaces. Second harmonic generation (SHG) laser imaging is a non-invasive and non-contact method, thus presenting an attractive alternative. However, the association between SHG-based measures and cartilage quality has not been established systematically. Here, we investigated the feasibility of using image-based measures derived from SHG microscopy for objective evaluation of cartilage quality as assessed by mechanical testing. Human tibial plateaus harvested from nine patients were used. Cartilage mechanical properties were determined using indentation stiffness (Einst) and streaming potential-based quantitative parameters (QP). The correspondence of the cartilage electromechanical properties (Einst and QP) and the image-based measures derived from SHG imaging, tissue thickness and cell viability were evaluated using correlation and logistic regression analyses. The SHG-related parameters included the newly developed volumetric fraction of organised collagenous network (Φcol) and the coefficient of variation of the SHG intensity (CVSHG). We found that Φcol correlated strongly with Einst and QP (ρ = 0.97 and - 0.89, respectively). CVSHG also correlated, albeit weakly, with QP and Einst, (|ρ| = 0.52-0.58). Einst and Φcol were the most sensitive predictors of cartilage quality whereas CVSHG only showed moderate sensitivity. Cell viability and tissue thickness, often used as measures of cartilage health, predicted the cartilage quality poorly. We present a simple, objective, yet effective image-based approach for assessment of cartilage quality. Φcol correlated strongly with electromechanical properties of cartilage and could fuel the continuous development of SHG-based arthroscopy.
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Cartílago Articular , Microscopía de Generación del Segundo Armónico , Humanos , Estudios de Factibilidad , Colágeno/análisis , Matriz Extracelular/químicaRESUMEN
Tissue swelling represents an early sign of osteoarthritis, reflecting osmolarity changes from iso- to hypo-osmotic in the diseased joints. Increased tissue hydration may drive cell swelling. The opposing cartilages in a joint may swell differently, thereby predisposing the more swollen cartilage and cells to mechanical injuries. However, our understanding of the tissue-cell interdependence in osmotically loaded joints is limited as tissue and cell swellings have been studied separately. Here, we measured tissue and cell responses of opposing patellar (PAT) and femoral groove (FG) cartilages in lapine knees exposed to an extreme hypo-osmotic challenge. We found that the tissue matrix and most cells swelled during the hypo-osmotic challenge, but to a different extent (tissue: <3%, cells: 11%-15%). Swelling-induced tissue strains were anisotropic, showing 2%-4% stretch and 1%-2% compression along the first and third principal directions, respectively. These strains were amplified by 5-8 times in the cells. Interestingly, the first principal strains of tissue and cells occurred in different directions (60-61° for tissue vs. 8-13° for cells), suggesting different mechanisms causing volume expansion in the tissue and the cells. Instead of the continuous swelling observed in the tissue matrix, >88% of cells underwent regulatory volume decrease to return to their pre-osmotic challenge volumes. Cell shapes changed in the early phase of swelling but stayed constant thereafter. Kinematic changes to tissue and cells were larger for PAT cartilage than for FG cartilage. We conclude that the swelling-induced deformation of tissue and cells is anisotropic. Cells actively restored volume independent of the surrounding tissues and seemed to prioritize volume restoration over shape restoration. Our findings shed light on tissue-cell interdependence in changing osmotic environments that is crucial for cell mechano-transduction in swollen/diseased tissues.
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Cartílago Articular , Condrocitos , Presión Osmótica , Condrocitos/fisiología , Concentración Osmolar , ÓsmosisRESUMEN
ABSTRACT: Zukowski, MH, Jordan, MJ, and Herzog, W. Modeling the early and late cceleration phases of the sprint start in elite long track speed skaters. J Strength Cond Res 38(2): 236-244, 2024-This study established the reliability of an exponential function to model the change in velocity during the speed skating sprint start and the validity of associated model parameters in a group of subelite and elite long track speed skaters. Long track speed skaters ( n = 38) performed maximal effort 50-m on-ice accelerations from a standing start while tethered to a horizontal robotic resistance device that sampled position and time data continuously. An exponential function was applied to the raw data to model the change in velocity throughout the acceleration phase and compute the maximal skating speed (MSS), maximal acceleration capacity (MAC), maximum relative net horizontal power ( PMax ), and an acceleration-time constant ( τ ). All constructed models provided a sufficient fit of the raw data ( R -squared > 0.95, mean bias <2%). Intraday reliability of all model parameters ranged from good to excellent (intraclass correlation coefficient >0.8 and coefficient of variation <5%). Strong negative correlations ( r : -0.72 to -0.96) were observed between MSS and PMax and the 10 and 20 m split times measured with the robotic resistance and with 100 split times obtained from 500 m races. Moderate-to-large between-group differences were observed in MSS, MAC, and PMax between the elite vs. subelite speed skaters (Cohen d effect sizes: 1.18-3.53). Our results indicate that monoexponential modeling is a valid and reliable method of monitoring initial acceleration performance in elite level long track speed skaters.
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Rendimiento Atlético , Patinación , Humanos , Reproducibilidad de los Resultados , AceleraciónRESUMEN
Given the recently proposed three-filament theory of muscle contraction, we present a low-cost physical sarcomere model aimed at illustrating the role of titin in the production of active force in skeletal muscle. With inexpensive materials, it is possible to illustrate actin-myosin cross-bridge interactions between the thick and thin filaments and demonstrate the two different mechanisms by which titin is thought to contribute to active and passive muscle force. Specifically, the model illustrates how titin, a molecule with springlike properties, may increase its stiffness by binding free calcium upon muscle activation and reducing its extensible length by attaching itself to actin, resulting in the greater force-generating capacity after an active than a passive elongation that has been observed experimentally. The model is simple to build and manipulate, and demonstration to high school students was shown to result in positive perception and improved understanding of the otherwise complex titin-related mechanisms of force production in skeletal and cardiac muscles.NEW & NOTEWORTHY Our physical sarcomere model illustrates not only the classic view of muscle contraction, the sliding filament and cross-bridge theories, but also the newly discovered role of titin in force regulation, called the three-filament theory. The model allows for easy visualization of the role of titin in muscle contraction and aids in explaining complex muscle properties that are not captured by the traditional cross-bridge theory.
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Actinas , Sarcómeros , Humanos , Sarcómeros/fisiología , Conectina/metabolismo , Contracción Muscular/fisiología , Músculo EsqueléticoRESUMEN
Objectives: The study aimed at discovering the existing differences in lower limb joints' kinematics, and EMG signals of 4 particular muscles of the ankle joint during gait, between normal subjects and patients with bilateral triple arthrodesis. Methods: In this research, a 3D motion analysis system was used and joints' angles were calculated using a MATLAB code, and based on the data collected from markers movements, for patients with bilateral triple arthrodesis and normal subjects. Moreover, the EMG signals of ankle muscles in each subject, and the graphs of mean plus and minus standard deviation of lower limb joint angles and muscles' EMG were calculated by MATLAB. Results: In all patients, an initial ankle eversion and valgus deformity were observed in their knee joints. In addition, for all patients, the maximum knee extension was less than that of the average value of the normal subjects. Furthermore, the results of the electromyography showed that, in all patients, delay occurred in gastrocnemius and soleus muscles in maximum contraction in their EMG signals. Besides, during the early stance phase of gait cycles, the mean value of EMG of peroneus brevis muscle for patients was more than that of normal subjects. Conclusion: Atrophy of four ankle muscles including (soleus, lateral gastrocnemius, tibialis anterior and peroneus brevis), also limitation of joints movement were observed in patients, compared to normal subjects. Based on the results of this work, in order to reduce further musculoskeletal disorders in patients who underwent bilateral triple arthrodesis surgery, there is a serious need to use physiotherapy after the surgery.
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Multiscale models aiming to connect muscle's molecular and cellular function have been difficult to develop, in part, due to a lack of self-consistent multiscale data. To address this gap, we measured the force response from single skinned rabbit psoas muscle fibers to ramp shortenings and step stretches performed on the plateau region of the force-length relationship. We isolated myosin from the same muscles and, under similar conditions, performed single molecule and ensemble measurements of myosin's ATP-dependent interaction with actin using laser trapping and in vitro motility assays. We fit the fiber data by developing a partial differential equation model that includes thick filament activation, whereby an increase in force on the thick filament pulls myosin out of an inhibited state. The model also includes a series elastic element and a parallel elastic element. This parallel elastic element models a titin-actin interaction proposed to account for the increase in isometric force following stretch (residual force enhancement). By optimizing the model fit to a subset of our fiber measurements, we specified seven unknown parameters. The model then successfully predicted the remainder of our fiber measurements and also our molecular measurements from the laser trap and in vitro motility. The success of the model suggests that our multiscale data are self-consistent and can serve as a testbed for other multiscale models. Moreover, the model captures the decrease in isometric force observed in our muscle fibers after active shortening (force depression), suggesting a molecular mechanism for force depression, whereby a parallel elastic element combines with thick filament activation to decrease the number of cycling cross-bridges.
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Mechanical power is a key performance indicator in long track speed skating. Maximal power output in athletic performance can be achieved when mechanical properties of muscles, such as the force-length relationship, are optimized. The purpose of this study was to determine the in vivo operating range of vastus lateralis (VL) fascicle lengths during speed skating imitation and compare the fascicle lengths to those that define the VL force-length relationship. Sixteen sub-elite long track speed skaters (7 females and 9 males; body mass: 72.5 [11.5] kg; age: 22.1 [2.7] years) performed maximal voluntary isometric knee extensions at nine different knee joint positions (20-120°) on the left leg to obtain the maximal vastus lateralis (VL) force-length relationship. Participants then performed a speed skating imitation exercise, the turn-cable, at three progressive perceived efforts (50%, 75%, 100%) to identify the VL fascicle excursion during a complete imitation skating stroke. Fascicle lengths and knee joint angles were examined at initial-contact, peak EMG, and take-off. Fascicles between initial contact and peak EMG covered the descending limb of both the maximal and submaximal force-length relationships while operating over the plateau region from peak EMG to take-off. We conclude that the VL works at sub-optimal length during the gliding phase of skating, but at optimal length for maximal force production during the crucial push-off phase where propulsion is provided.