A new strategy to simultaneous removal and recovery of nitrogen from wastewater without N2O emission by heterotrophic nitrogen-assimilating bacterium.

Biological assimilation that recovery the nitrogen from wastewater in the form of biomass offers a more environmentally friendly solution for the limitations of the conventional wastewater treatments. This study reported the simultaneous removal and recovery of nitrogen from wastewater without N2O emission by a heterotrophic nitrogen-assimilating Acinetobacter sp. DN1 strain. Nitrogen balance, biomass qualitative analysis, genome and enzyme studies have been performed to illustrate the mechanism of nitrogen conversion by strain DN1. Results showed that the ammonium removal followed one direct pathway (GOGAT/GDH) and three indirect pathways (NH4+ → NH2OH → NO → NO2- → NH4+ → GOGAT/GDH; NH4+ → NH2OH → NO → NO2- → NO3- → NO2- → NH4+ → GOGAT/GDH; NH4+ → NH2OH → NO → NO3- → NO2- → NH4+ → GOGAT/GDH). Nitrogen balance and biomass qualitative analysis showed that over 70 % of the ammonium in the wastewater was converted into intracellular nitrogen-containing compounds and stored in the cells of strain DN1. Traditional denitrification pathway was not detected and the ammonium was removed through assimilation, which makes it more energy-saving for nitrogen recovery when compared with Haber-Bosch process. This study provides a new direction for simultaneous nitrogen removal and recovery without N2O emission by the heterotrophic nitrogen-assimilating bacterium.

Integrative chemical and omics analysis of the ammonia nitrogen removal characteristics and mechanism of a novel oligotrophic heterotrophic nitrification-aerobic denitrification bacterium.

A novel oligotrophic heterotrophic nitrification-aerobic denitrification bacterium designated as Pseudomonas sp. N31942, was isolated from a eutrophic lake. Strain N31942 exhibits high ammonia nitrogen removal ability in oligotrophic environment as ammonia nitrogen can be efficiently (86.97 %) removed within 10 h with no accumulation of nitrite. In the nitrification process, strain N31942 can convert ammonia into nitrate in the absence of hydroxylamine oxidase and nitrite oxidoreductase. As for the denitrification process, nitrate or nitrite were reduced to ammonia and further converted into glutamate by dissimilatory nitrate reduction pathway. Transcriptomic analysis detected 2080 differentially expressed genes. Among them, the expression of the related genes in dissimilatory nitrate reduction process was all up-regulated at low ammonia concentrations, which indicates that the strain has excellent nitrogen removal efficiency for further nitrogen removal. Integrative omics analyses revealed that strain N31942 may have two possible pathways for the NH4+-N removal as direct GDH/GS-GOGAT pathway (NH4+-N → Glutamate) and indirect GDH/GS-GOGAT pathway (NH4+-N → NH2OH → NO2--N → NO3--N → NO2--N → NH4+-N → Glutamate). Moreover, strain N31942 also has excellent nitrogen removal ability for real sewage and 77.21 % total nitrogen could be removed within 48 h. The results presented here provide new insights into ammonia nitrogen removal characteristics and mechanism of heterotrophic nitrification-aerobic denitrification bacterium under oligotrophic conditions.

Enhanced Visible-Light Absorption of Fe2O3 Covered by Activated Carbon for Multifunctional Purposes: Tuning the Structural, Electronic, Optical, and Magnetic Properties.

Visible-light absorption is a critical factor for photocatalyst activity and absorption of electromagnetic (EM) interference application. The band gap of Fe2O3 is 2 eV, which can be increased by doping with a high-band-gap material such as carbon from activated carbon (AC) with a band gap of 4.5 eV for increased visible-light absorption. The porosity decreases from 88 to 81.6%, and the band gap increases from 2.14 to 2.64 eV by increasing the AC from 10 to 25%, respectively. The photocatalytic activity takes 120 min to produce a harmless product for 10-20% AC, but 25% AC shows 89.5% degradation in only 90 min and the potential to attenuate the EM wave up to 99% due to the RL being below -20 dB. The second- and third-cycle degradation achieved by the composite Fe2O3-AC having 25% AC is 88.2 and 86.5% in 90 min, respectively. The pore of the surface state of AC contains a trapped charge, and interaction occurs between the charge (electron/hole) and O2 or H2O to produce OH and superoxide (O2 -) radicals. These radicals move inside the molecule of the pollutant (methylene blue (MB)) to break up the bond, with the final products being H2O and CO2. The X-ray photoelectron (XPS) spectra show that oxygen plays a key role in the interatomic bonding with Fe, C, and MB atoms. The best absorption of EM interference is -21.43 dB, with degradation reaching 89.51% in only 90 min for 25% AC due to its higher band gap and anisotropy constant. Fe2O3-carbon is a multifunctional material for the green environment because of its electromagnetic interference absorption and photodegradation of wastewater.