Conjugated Oligoelectrolyte with DNA Affinity for Enhanced Nuclear Imaging and Precise DNA Quantification.

Precise DNA quantification and nuclear imaging are pivotal for clinical testing, pathological diagnosis, and drug development. The detection and localization of mitochondrial DNA serve as crucial indicators of cellular health. We introduce a novel conjugated oligoelectrolyte (COE) molecule, COE-S3, featuring a planar backbone composed of three benzene rings and terminal side chains. This unique amphiphilic structure endows COE-S3 with exceptional water solubility, a high quantum yield of 0.79, and a significant fluorescence Stokes shift (λex = 366 nm, λem = 476 nm), alongside a specific fluorescence response to DNA. The fluorescence intensity correlates proportionally with DNA concentration. COE-S3 interacts with double-stranded DNA (dsDNA) through an intercalation binding mode, exhibiting a binding constant (K) of 1.32 × 106 M-1. Its amphiphilic nature and strong DNA affinity facilitate its localization within mitochondria in living cells and nuclei in apoptotic cells. Remarkably, within 30 min of COE-S3 staining, cell vitality can be discerned through real-time nuclear fluorescence imaging of apoptotic cells. COE-S3's high DNA selectivity enables quantitative intracellular DNA analysis, providing insights into cell proliferation, differentiation, and growth. Our findings underscore COE-S3, with its strategically designed, shortened planar backbone, as a promising intercalative probe for DNA quantification and nuclear imaging.

[The Erythrocyte Deformability Measuring System Based on Microlfuidic and Machine Vision].

The erythrocyte deformability mainly depends on the composition of the membrane and cytoplasm, which consist of hemoglobin and other constituents. The most of current devices to measure erythrocyte deformability require washing process after the measurement which is labor-intensive and time-consuming. This article introduces an improved measuring system based on microfluidic and machine vision (RSD) which adopts disposable microfluidic channel and conventional laser-diffraction technique to determine erythrocyte deformability. To validate the accuracy and repeatability of RSD, several experiments are conducted to determine deformability of normal erythrocytes measured by RSD and a conventional ektacytometer(LORCA). The measured Elongation Index (EI) by RSD, which is a parameter directly related to erythrocyte deformability, is in excel ent agreement with it measured by LORCA.

[Application of Electromagnetic Scanning Technique to EMC Countermeasure for Medical Electrical Equipment].

With practical cases, this paper is to show the major application and according advantages in EMC countermeasure for medical electrical equipment, for instance, rapid fully-scanning, stability to ensure repeatability, precise location of interference source and efficient countermeasure. It is likely helpful and significant to related test being conducted successfully and effectively.

A transient, microfluidic approach to the investigation of erythrocyte aggregation: the threshold shear-stress for erythrocyte disaggregation.

Detailed analysis of red blood cells (RBCs) aggregation is often required in various clinical studies. Most conventional aggregation indices are dimensionless values and not available for comparison of across studies. Quite recently, we have developed microfluidic aggregometry that enables us to yield a critical shear-stress that are required to aggregate RBCs under the shearing hydrodynamic force. The present study investigated the relationships between the values of the critical shear-stress and conventional aggregation indices by comparing the critical shear-stress measured by the microfluidic aggregometry with the threshold shear-stress measured using a LORCA aggregometer. The results showed that the critical shear-stress did not vary with the hematocrit value while the threshold shear-rate decreased with the hematocrit value. The threshold shear-stress also showed the same hematocrit-independence as the critical shear-stress. These findings assist in rheologically validating the critical shear-stress, as defined in the microfluidic aggregometry, within the present range of hematocrit values.