RESUMEN
OBJECTIVE: This study aims to investigate the impact of surgical experimental variables on the prognosis of orthotopic liver transplantation (OLT) in rats, with the goal of enhancing the efficacy of modeling techniques. METHODS: Using Kamada's "two-cuff method" of rat orthotopic liver transplantation, 76 pairs of SD-Wistar rats were performed orthotopic liver transplantation from March to September 2023. Thirteen experimental factors during the perioperative period and the survival time of recipient rats were collected and recorded. To explore the surgical factors affecting the prognosis of rat liver transplantation and summarize the surgical techniques. RESULTS: The success rate of orthotopic liver transplantation in SD-Wistar rats was 68.4%, with 24 recipients surviving within 3-7 days and 28 recipients surviving more than 1 week. Donor liver perfusion, recipient blood loss, recipient liver blood expulsion, anhepatic phase, suprahepatic inferior vena cava anastomosis time and anesthesia recovery time are related to the survival of recipient rats after liver transplantation. Donor liver perfusion, eliminating blood in recipient liver and intraoperative blood loss of recipient are surgical factors affecting the prognosis of liver transplantation in rats. The survival time of recipient rats with liver perfusion through abdominal aorta, eliminating blood in recipient liver was relatively prolonged after operation. CONCLUSION: Under the condition of reasonable control of the anhepatic phase, the perfusion method of the donor liver, whether to eliminate blood in recipient liver, and intraoperative blood loss of recipient are important surgical factors affecting the prognosis of liver transplantation in rats.
