pH-sensitive chitosan-derived nanoparticles as doxorubicin carriers for effective anti-tumor activity: preparation and in vitro evaluation.

pH-sensitive self-aggregated nanoparticles (SNPs), based on amphiphilic deoxycholic acid (DOCA) modified carboxymethyl chitosan (DCMC), were prepared for delivery of the anticancer drug doxorubicin (DOX). DCMCs with different degrees of substitution (DS) of DOCA were initially synthesized and characterized. Based on self-aggregation, DCMC formed nanoparticles with size ranging from 87 to 174 nm. The critical aggregation concentration (CAC) decreased on increasing the DS of DOCA. Moreover, the DCMC SNPs showed an acidic pH-induced aggregation and deformation behavior. The DOX-loaded SNPs ([D]NP) exhibited a sustained drug release manner, which could be accelerated by an acidic pH, but delayed by a higher DS of DOCA. Antitumor efficacy results showed that [D]NP could suppress both sensitive and resistant MCF-7 cells effectively in a dose- and time-dependent manner. The enhanced cellular uptake and greater retention of [D]NP in drug-resistant cells, as evidenced by confocal microscopy and flow cytometry, contributed to a superior efficacy of [D]NP over free DOX. These results suggest the potential of DCMC SNPs as carriers for the hydrophobic drug DOX for effective cancer therapy against drug-resistant tumors.

[Soil organic carbon fractionation methods and their applications in farmland ecosystem research: a review].

Soil organic carbon is of heterogeneity in components. The active components are sensitive to agricultural management, while the inert components play an important role in carbon fixation. Soil organic carbon fractionation mainly includes physical, chemical, and biological fractionations. Physical fractionation is to separate the organic carbon into active and inert components based on the density, particle size, and its spatial distribution; chemical fractionation is to separate the organic carbon into various components based on the solubility, hydrolizability, and chemical reactivity of organic carbon in a variety of extracting agents. In chemical fractionation, the dissolved organic carbon is bio-available, including organic acids, phenols, and carbohydrates, and the acid-hydrolyzed organic carbon can be divided into active and inert organic carbons. Simulated enzymatic oxidation by using KMnO4 can separate organic carbon into active and non-active carbon. Biological fractionation can differentiate microbial biomass carbon and potential mineralizable carbon. Under different farmland management practices, the chemical composition and pool capacity of soil organic carbon fractions will have different variations, giving different effects on soil quality. To identify the qualitative or quantitative relationships between soil organic carbon components and carbon deposition, we should strengthen the standardization study of various fractionation methods, explore the integrated application of different fractionation methods, and sum up the most appropriate organic carbon fractionation method or the appropriate combined fractionation methods for different farmland management practices.

Preparation and characterization of ß-elemene-loaded microemulsion.

Intravenously injectable emulsion of ß-elemene was studied in detail. Both blank and ß-elemene-loaded microemulsions were prepared using a simple water titration method. The pseudoternary phase diagram was constructed for the optimization of microemulsion. The loading capacity test, dilutability test, and especially the influence of antioxidants were conducted for further optimization of ß-elemene-loaded microemulsion. Transmission electron microscope showed intact and spherical microemulsion droplets. Conductivity and viscosity measurements were used to study the phase behaviors of ß-elemene-loaded microemulsions, providing convincing explanation. In vitro release study showed that ß-elemene was steadily released until 12 h, which most fitted the first order.

[Effects of vegetation restoration on soil microbial biomass carbon and nitrogen in hilly areas of Loess Plateau].

Aimed to explore the effects of different vegetations and of the years of vegetation restoration on soil microbial biomass carbon and nitrogen, a comparative study was conducted, with the 5 year old Robinia pseudoacacia, Hippophae reamnoide and Prunus armeniaca plantations and the 5, 15 and 25 years old R. pseudoacacia plantation in the Yangjuangou catchment of Yanan City of Shaanxi Province, a typical hilly area of the Loess Plateau, as test objects. The results showed that among the three 5-year old plantations, H. reamnoides plantation had the highest soil organic carbon (SOC) and total nitrogen (TN) contents, while R. pseudoacacia plantation had the highest soil microbial biomass carbon (MBC) (99.56 mg x kg(-1)) and nitrogen (MBN) (28.81 mg x kg(-1)). The MBC was in the order of R. pseudoacacia > H. reamnoides > P. armeniaca, and that of MBN was of R. pseudoacacia > P. armeniaca > H. reamnoides. The MBC/SOC was in the order of R. pseudoacacia > H. reamnoides > P. armeniaca, and that of MBN/TN was of R. pseudoacacia > P. armeniaca > H. reamnoides, with the differences being significant (P < 0.05). With the increasing years of vegetation restoration, the soil pH in R. pseudoacacia plantation decreased, while the SOC, TN, electricity conductance (EC), MBC, and MBN all had an increasing trend, which illustrated that in the hilly area of Loess Plateau, planting R. pseudoacacia was more beneficial to the increase of soil MBC and MBN, and, with the increasing years of this planting, soil MBC, MBN, SOC and TN tended to be increasing.