Regulatory potential of secondary metabolite DIMBOA and baicalein to imazethapyr-induced toxicity in wheat seedlings.

Controlling and mitigating the toxicity of herbicides to non-target plants is of significant importance in reducing ecological risks. The development of green and natural herbicide control technologies has become an urgent necessity. In this paper, how 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazine-3(4H)-one (DIMBOA) and baicalein alleviated oxidative stress induced by imazethapyr (IM) in wheat seedlings was investigated. We found that DIMBOA and baicalein enhanced the antioxidant enzyme activities in wheat seedlings exposed to IM and reduced the excessive reactive oxygen species due to IM stress by 21.3% and 23.5%, respectively. DIMBOA and baicalein also restored the iron content reduced by IM and effectively mitigated Fe2+ overload by alleviating the response of heme oxygenase 1 to IM stress. The antioxidant and iron homeostatic maintenance properties of DIMBOA and baicalein enhanced the defenses of wheat seedlings against IM stress. Our results highlight the potential implication of secondary metabolites as natural products to modulate herbicide toxicity to non-target plants.

Phytotoxicity alleviation of imazethapyr to non-target plant wheat: active regulation between auxin and DIMBOA.

Effectively controlling target organisms while reducing the adverse effects of pesticides on non-target organisms is a crucial scientific inquiry and challenge in pesticide ecotoxicology research. Here, we studied the alleviation of herbicide (R)-imazethapyr [(R)-IM] to non-target plant wheat by active regulation between auxin and secondary metabolite 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazine-3(4H)-one (DIMBOA). We found (R)-IM reduced 32.4% auxin content in wheat leaves and induced 40.7% DIMBOA accumulation compared to the control group, which effortlessly disrupted the balance between wheat growth and defense. Transcriptomic results indicated that restoration of the auxin level in plants promoted the up-regulation of growth-related genes and the accumulation of DIMBOA up-regulated the expression of defense-related genes. Auxin and DIMBOA alleviated herbicide stress primarily through effects in the two directions of wheat growth and defense, respectively. Additionally, as a common precursor of auxin and DIMBOA, indole adopted a combined growth and defense strategy in response to (R)-IM toxicity, i.e., restoring growth development and enhancing the defense system. Future regulation of auxin and DIMBOA levels in plants may be possible through appropriate methods, thus regulating the plant growth-defense balance under herbicide stress. Our insight into the interference mechanism of herbicides to the plant growth-defense system will facilitate the design of improved strategies for herbicide detoxification.

Positive Emotion and Media Dependence: Measuring Risk Information Seeking and Perception in the COVID-19 Pandemic Prevention.

Risk perception and information seeking behaviors are affected by individual psychological and situational factors. In the background of COVID-19 prevailing for a long period, this study examined Chinese people's information seeking and processing behavior by the RISP model, which focused on the impact of individual risk perception, affective response, perceived information-gathering capacity, and media trust and the impact of the above factors on information seeking. This study designed an online survey with gender and age quotas among the Chinese population, including a total of 675 valid samples. It was found that the Chinese public's risk perception to pandemic had a positive effect on perceived information-gathering capacity and media trust. Furthermore, both positive emotional responses and negative emotional responses had a positive effect on information seeking behavior. Nurturing positive emotion engendered a holistic perception in pandemic information seeking. In addition, media trust, perceived information-gathering capacity, and subjective norms also positively impact information seeking behavior.

Enantioselective Response of Wheat Seedlings to Imazethapyr: From the Perspective of Fe and the Secondary Metabolite DIMBOA.

The responses of trace elements and secondary metabolites to stress can reflect plant adaptation to the environment. If and how the imperative trace element Fe and the defensive secondary metabolite 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazine-3(4H)-one (DIMBOA) mediate the toxicity of chiral herbicides to nontarget plants remains inconclusive. We found that the herbicidal-active imazethapyr enantiomer [(R)-IM] stimulated heme oxygenase-1 activity, triggered the release of the catalytic product Fe2+, increased reactive oxygen species production, decreased the DIMBOA content, and increased the DIMBOA-Fe content. XAFS analyses and in vitro Fenton assays demonstrated that DIMBOA could relieve phytotoxicity by chelating excessive Fe3+ to restore Fe homeostasis. The free radical scavenging ability of the chelate of DIMBOA and Fe was also involved. This work refines the dual role of DIMBOA and Fe in mediating the enantioselective phytotoxicity of chiral herbicides, which provides a new direction for improving the herbicide resistance of crops.

New Target in an Old Enemy: Herbicide (R)-Dichlorprop Induces Ferroptosis-like Death in Plants.

Iron is an essential microelement in plants that is involved in several growth processes. The use of herbicides may cause the abnormal aggregation of iron in leaves, but the regulatory mechanisms underlying this phenomenon remain unclear. Here, we show that chiral herbicide (R)-dichlorprop ((R)-DCPP) triggers ferroptosis-like death in Arabidopsis thaliana. (R)-DCPP led to reactive oxygen species (ROS) accumulation and iron aggregation, and these processes were iron dependent. Under (R)-DCPP treatment, ROS, lipid hydrogen peroxides, and malondialdehyde were significantly accumulated. In addition, (R)-DCPP induced the depletion of glutathione, ascorbic acid, and glutathione peroxidase as well as the accumulation of toxic lipid peroxides. Thus, oxidation imbalance led to cell death, and this mode of action could be inhibited by the ferroptosis inhibitor ferrostatin-1 or ciclopirox olamine. NADPH oxidases were found to be involved in herbicide-induced ROS accumulation, and lipoxygenase and NADPH cytochrome P450 oxidase were shown to positively regulate (R)-DCPP-induced lipid peroxidation. Overall, these results indicate that the iron- and ROS-dependent signaling cascades were involved in the (R)-DCPP-induced phytotoxicity pathway, which disrupted the structure of plant cell membranes and triggered ferroptosis. Generally, this study provides new insight into the mechanisms of pesticide phytotoxicity and suggests new therapeutic directions to protect nontarget plants.

A sandwich model of Cr(VI) adsorption and detoxification by Fenton modified chitosan.

The Fenton reaction has the advantages of short reaction time, low cost, no toxicity, and straightforward application and control. The Fenton reaction generates highly reactive HO•, which has been applied effectively. However, the effect of the generated Fe3+ has not been investigated widely. In this study, the Fenton reaction was used to improve the Cr(VI) adsorption and detoxification capacities of chitosan. After the Fenton modification, chitosan efficiently adsorbed Cr(VI) and transformed it into the less toxic Cr(III) in a wide pH range as a result of layer formation, which was described by a sandwich model. The adsorption of Cr(VI) onto the Fenton modified chitosan was in good agreement with the Freundlich adsorption model, and the adsorption capacity exceeded 120 mg/g. During the Fenton reaction, H2 O2 and HO• with high oxidative activity broke the hydrogen bonds in the chitosan structure, resulting in the release of free amine groups for Fe3+ to form metal-binding biopolymers. The distance between the chitosan polymers increased, and additional adsorption sites were created. HCrO4 - entered the gap between the chitosan polymer and was adsorbed on the newly created adsorption sites. The sandwich adsorption model indicated that the Fenton modified chitosan provided a high concentration of active sites for Cr(VI) capture and detoxification. PRACTITIONER POINTS: Fenton reaction was used to improve the adsorption ability of chitosan. The formed Fe3+ in Fenton reaction was utilized. HO· broke the hydrogen bonds and Fe3+ ions chelated with chitosan in modification. Cr(VI) could be adsorbed and reduced efficiently by Fenton modified chitosan. The Fenton modified chitosan provided a high concentration of active sites for Cr(VI) capture and detoxification.