RESUMEN
After undergoing inhalation anesthesia, some patients, especially elderly patients, experience postoperative cognitive dysfunction, such as personality changes and memory impairment. In the present study, 20-month-old rats were randomly allocated to sevoflurane (Sevo group) and control groups (Con group), and they inhaled 3% sevoflurane or 40% oxygen for 8 hours, respectively. The Morris water maze test found that the cognitive function of rats in the Sevo group were significantly different on 1d and 3d after anesthesia than that of rats in the Con group. The expression of RGS2 mRNA and protein in hippocampus of Sevo group was lower compared to the Con group, while Ca2 + was higher than con group. The expression of CaM and CaMK II in Sevo group was higher compared to the Con group. We found that Bcl-2 reduced, but the expression of Bax and Caspase-3 increased, indicating that apoptosis of hippocampal neurons was increased after sevoflurane inhalation. Both the expression of NGF and BDNF was depressed in the Sevo group. After continuous inhalation of 3% sevoflurane for 8h, the expression of RGS2 in the hippocampi of aged rats is down regulated. RGS2 may be an important factor that leads to cognitive dysfunction in rats.
Asunto(s)
Disfunción Cognitiva/inducido químicamente , Proteínas RGS/metabolismo , Sevoflurano/toxicidad , Envejecimiento , Animales , Calcio/metabolismo , Proteína Quinasa Tipo 2 Dependiente de Calcio Calmodulina/genética , Proteína Quinasa Tipo 2 Dependiente de Calcio Calmodulina/metabolismo , Regulación de la Expresión Génica/efectos de los fármacos , Hipocampo/efectos de los fármacos , Hipocampo/metabolismo , Prueba del Laberinto Acuático de Morris , Proteínas Proto-Oncogénicas c-bcl-2/genética , Proteínas Proto-Oncogénicas c-bcl-2/metabolismo , Proteínas RGS/genética , Ratas , Ratas Wistar , Proteína X Asociada a bcl-2/genética , Proteína X Asociada a bcl-2/metabolismoRESUMEN
SummaryPropofol is a intravenous anaesthetic most commonly used in ultrasound oocyte retrieval. We studied if the use of propofol had an effect on mouse oocyte maturation, pregnancy, childbirth and progeny and investigated the correlation between propofol side effects and reproductive performance in mice. There was no statistical difference in mating, pregnancy, childbirth, litter size, the number of stillbirths and survival between each group (P>0.05). Propofol also had no effect on polar body extrusion in oocyte maturation as well as on pronucleus formation and, subsequently, early embryo development (P>0.05). An increased concentration of propofol had no effect on this result, although propofol at more than 0.01 mg/ml reduced polar body extrusion. Different concentrations of propofol had no effect on oocyte culture in vitro, pronucleus formation and early embryo development.
