1.
Chem Commun (Camb)
; 56(45): 6114-6117, 2020 Jun 07.
Artículo
en Inglés
| MEDLINE
| ID: mdl-32363374
RESUMEN
Native chemical ligation (NCL) enables the chemical synthesis of peptides via reactions between N-terminal thiolates and C-terminal thioesters under mild, aqueous conditions at pH 7-8. Here we demonstrate quantitatively how thiol speciation at N-terminal cysteines and analogues varies significantly depending upon structure at typical pH values used in NCL.