Uptake of selected antiretrovirals by pepper (Capsicum annum), radish (Raphanus sativus), and ryegrass (Lolium perenne) grown on two contrasting soils and fertilized with human urine-derived fertilizers.

The use of urine-derived fertilizers has several economic and environmental advantages. However, there is concern that pharmaceutical residues present in urine could enter the food chain after plant uptake and pose potential risks to human and animal health. A pot experiment was conducted to evaluate the uptake of nine target antiretroviral drugs (ARVDs) by pepper (Capsicum annum), ryegrass (Lolium perenne) and radish (Raphanus sativus) grown in two soils of contrasting texture and organic matter content and fertilized with stored urine, nitrified urine concentrate (NUC), and struvite. Nevirapine was the only ARVD detected in crops grown with NUC and struvite on both soils, but the concentrations were below the limit of quantification. Plants fertilized with stored urine absorbed lamivudine, ritonavir, stavudine, emtricitabine, nevirapine, and didanosine, while abacavir, efavirenz and zidovudine were not detected. The ARVDs detected in the soils after harvest were significantly higher in the soil with high organic matter and clay content. To assess direct human exposure the estimated daily dietary intake (DDI) of ARVDs by consumption of the pepper and radish fertilized with stored urine was compared with the Threshold of Toxicological Concern (TTC) values based on the Cramer classification tree. The calculated DDI values for all ARVDs were about 300-3000 times lower than the TTC values for class III compounds. Therefore, daily consumption of these crops fertilized with stored urine does not pose a health risk to the consumer. Future research is required to assess the impact of ARVD metabolites, which may be more harmful to human health than the parent compounds.

Total organic carbon, aluminium and iron in bulk samples and aggregate size fractions of a sandy clay loam humic soil under sugarcane relative to native forest in northern KwaZulu-Natal, South Africa.

The distribution of total aluminium (Al) and iron (Fe) and organic carbon (TOC) in aggregate fractions gives an insight into the dynamics of these elements in soil. This study determined the effects of over 30 years of sugarcane cultivation, compared to adjacent native forest, on Al, Fe and TOC in bulk soil and aggregate fractions within the 100 cm depth of a sandy clay loam humic soil. Samples were separated into large macro-aggregates: LM (>2000 µm), small macro-aggregates: SM (250-2000 µm), micro-aggregates: M (250-63 µm) and silt + clay: SC (<63 µm) fractions. The TOC was analyzed by dry combustion and total Al and Fe by X-ray fluorescence spectrometry. Sugarcane cultivation (i) reduced macro-aggregates and TOC and (ii) increased the SC fraction and total Al and Fe. The mean weight diameter declined from 1.32 mm (0-30 cm) to 1.06 mm (30-100 cm) under forest. Average (0-100 cm) Al and Fe contents (g kg-1) increased in LM (6-16 for Al; 6 to 9 for Fe), SM (7-11 for Al), M (5-14 for Al; 6 to 9 for Fe) and SC (7-16 for Al; 9 to 10 for Fe) under sugarcane relative to forest. The TOC (g kg-1) declined in the LM (13-7) and SM (7-6) but increased in the M (5-9) and SC (10-13) due to cultivation. These findings suggested that sugarcane cultivation decreases aggregate stability and TOC in macro-aggregates, and increases Al and Fe in all aggregates. Adoption of practices inclined to improve or maintain TOC as well as liming to increase pH are necessary management practices for sustainable production.

Land use and site effects on the distribution of carbon in some humic soil profiles of KwaZulu-Natal, South Africa.

Land use effects on the stocks of soil organic carbon (SOC) are generally based on the topsoil. Although subsoil horizons have lower concentrations, they contain a significant amount of SOC which may be more strongly protected than that in the topsoil layers. Analysis of SOC storage must therefore include the whole profile in respect of climate change mitigation. Humic soils in South Africa have high organic C in the A horizon, while the amount of C stocks through the whole profile depth is unknown. This study was conducted at six sites in KwaZulu-Natal Province to determine the effect of land use and site factors on C stocks, texture, pH and extractable aluminium (Al) and iron (Fe) concentrations and their vertical distribution to 100 cm in soils with thick (>45 cm) and thin (<45 cm) humic A horizons. The land use at some sites had been changed from grassland to maize and cultivated pasture and at others from forest to sugarcane farming. Cultivation with field crops reduced the organic C, mainly in the upper 20 cm (from 110 to 22 g C kg-1), with limited effect in deeper layers. The soils with thick humic A horizons and coarser texture stored more C in the deeper layers compared to those with thin humic A horizons and finer texture which had more of the C stocks in the 0-20 cm depth. Although cultivation reduced the soil C stocks in the surface layers, land use did not significantly affect the overall C stock (0-100 cm) at all sites. The high contents of extractable Fe (up to 340 mg kg-1) and Al (up to 3700 mg kg-1) stabilised the soil C and were more important than the effects of either land use or other site factors.