RESUMEN
OBJECTIVES: To evaluate long-term changes in acetylcholinesterase (AChE) activity in CSF and blood following donepezil treatment in relation to the concentration of donepezil and cognition in AD patients. METHODS: CSF or blood (or both) samples of a total of 104 patients with mild AD were used [MMSE score 23 +/- 0.4; age 75 +/- 1 years (mean +/- SEM); n=53 for CSF and n=51 for plasma/red blood cell (RBC) samples]. The patients were treated with 5 or 10 mg/day donepezil and clinically followed for 2 years. The CSF and RBC AChE activities were measured by the Ellman's direct colorimetric assay. Protein levels of two variants of AChE ("read-through" AChE-R and synaptic AChE-S) were determined by an ELISA-like method. RESULTS: The plasma donepezil concentration was dose-dependent (between 30 and 60 ng/mL in the 5-mg and 10-mg group, respectively). The CSF donepezil concentration was 10 times lower than the plasma level and showed dose- and time-dependent kinetics. The RBC AChE inhibition was moderate (19-29%). CSF AChE-S inhibition was estimated to 30-40% in the 5-mg and 45-55% in the 10-mg group. Positive correlations were observed between the CSF AChE inhibition, an increased protein level of the AChE-R variant and MMSE examination. Patients with high AChE inhibition (>or=45%) showed a stabilized MMSE test result after up to two years, while a significant decline was observed in AD patients with lower AChE inhibition (Asunto(s)
Acetilcolinesterasa/efectos de los fármacos
, Enfermedad de Alzheimer/tratamiento farmacológico
, Inhibidores de la Colinesterasa/uso terapéutico
, Cognición/efectos de los fármacos
, Indanos/uso terapéutico
, Piperidinas/uso terapéutico
, Acetilcolinesterasa/líquido cefalorraquídeo
, Anciano
, Enfermedad de Alzheimer/líquido cefalorraquídeo
, Inhibidores de la Colinesterasa/análisis
, Cromatografía Líquida de Alta Presión
, Donepezilo
, Relación Dosis-Respuesta a Droga
, Eritrocitos/efectos de los fármacos
, Eritrocitos/enzimología
, Femenino
, Humanos
, Indanos/análisis
, Masculino
, Piperidinas/análisis
, Factores de Tiempo