Impact of Refractive Errors on the Academic Performance of High School Children of Lahore.

Introduction: The process of learning begins in childhood and accurate vision can greatly affects a child's learning capacity. It is documented that visual impairment in children can have a significant impact on their performance at school as well as their social interaction and development. Objective: This research aimed to study the impact of refractive corrections on the academic performance of high school children in Lahore. Methodology: A total of 2,000 students with equal distribution of gender, public, private school, and locality were included in the study. All students were screened for defective vision. The academic performance before and after corrections was recorded on the prescribed proforma. Results: The prevalence of refractive error was high among the public high schools 244 (59.2%) as compared to the private schools 168 (40.8%). The area-based prevalence was higher among the students in urban settings 255 (62%) while in rural it was 157 (38%). It was found that in the public sector, the average score of academic results before the intervention was 56.39 ± 13.24 which was increased to 60.27 ± 14.94 after the intervention while in the private sector, before the intervention, the average score was 63.53 ± 17.50 which was improved to 67.12 ± 18.48. It was found to be statistically significant at p-value < 0.05. Conclusion: A significant impact was observed in the average academic scores of the results after refractive corrections.

Delineating Novel and Known Pathogenic Variants in TYR, OCA2 and HPS-1 Genes in Eight Oculocutaneous Albinism (OCA) Pakistani Families.

Oculocutaneous albinism (OCA) is associated with a wide range of clinical presentations and has been categorized with syndromic and non-syndromic features. The most common causative genes in non-syndromic OCA are TYR and OCA2 and HSP1 is in the syndromic albinism. The objective of this study was to identify pathogenic variants in congenital OCA families from Pakistan. Eight consanguineous families were recruited, and clinical and ophthalmological examination was carried out to diagnose the disease. Whole blood was collected from the participating individuals, and genomic DNA was extracted for sequencing analysis. TruSight one-panel sequencing was carried out on one affected individual of each family, and termination Sanger sequencing was carried out to establish the co-segregation of the causative gene or genes. In silico analysis was conducted to predict the causative pathogenic variants. Two families were found to have novel genetic pathogenic variants, and six families harbored previously reported variants. One novel compound heterozygous pathogenic variant in the TYR gene, c.1002delA; p.Ala335LeufsTer20, a novel frameshift deletion pathogenic variant and c.832C>T; and p.Arg278Ter (a known pathogenic variant) were found in one family, whereas HPS1; c.437G>A; and p.Trp146Ter were detected in another family. The identification of new and previous pathogenic variants in TYR, OCA2, and HPS1 genes are causative of congenital OCA, and these findings are expanding the heterogeneity of OCA.

Comparison of effectiveness of WhatsApp and Facebook as learning tool for the students of a medical college.

OBJECTIVE: To compare the effectiveness of Facebook and WhatsApp as learning tools for undergraduate medical students in ophthalmology. METHODS: The quantitative, quasi-experimental study was conducted at Khawaja Muhammad Safdar Medical College, Sialkot, Pakistan from October 2017 to March 2018, and comprised 4th year medical students. A pre-test of ophthalmology topics was taken and participants were divided into Facebook and WhatsApp groups by gender-based stratified randomisation. Four topics were taught through Facebook and WhatsApp to the relevant groups and post-test was taken using multiple choice questions. Data was analysed using SPSS 20. RESULTS: Of the 100 students, 35(35%) were males and 65(65%) were females. The overall mean age was 21.76±0.85 years (range: 20-24 years of. The two groups had 50(50%) subjects each. A significant difference was found between pre- and post-test results of both the groups (p<0.001). There was no significant difference between the groups (p>0.05) neither the baseline nor post-intervention. There was significant difference along gender lines (p>0.05). CONCLUSION: Facebook and WhatsApp as learning tools had no significant difference in terms of impact on the learning process.

Antioxidant pretreatment enhances umbilical cord derived stem cells survival in response to thermal stress in vitro.

Aim: Pretreatment of stem cells with antioxidants accelerates their ability to counter oxidative stress and is associated with the overall therapeutic outcome of their transplantation. Material & methods: Wharton Jelly derived mesenchymal stem cells (WJMSCs) were cultured and pretreated with various doses of antioxidants; Vitamin C (Vit C), Vitamin E (Vit E), Vitamin D3 (Vit D3) and their Cocktail, followed by exposure to in vitro heat injury. Assessment of WJMSCs survival, paracrine release, in vitro wound healing and expression of angiogenic and survival markers was conducted. Results: The results displayed an enhanced survival of WJMSCs especially in the case of Cocktail priming. Conclusion: Our data suggest that antioxidant pretreatment of WJMSCs strengthens the endurance of the cells, within stress conditions.

Use of Smart Phones and Social Media in Medical Education: Trends, Advantages, Challenges and Barriers.

INTRODUCTION: The technological advancements have transformed the society into a global forum influencing the educational processes and learning environments. Medical education is no exemption with an increasing trend to use the social media and smart phones for teaching and learning. Facebook, WhatsApp, Instagram, YouTube and Edmodo are the platforms promoting collaborative learning, improved communication and knowledge sharing. AIM: This study aims to review the use of smart phones and social media in the context of medical education. It reviews the usage of smart phone and social medical applications including Facebook, WhatsApp and Edmodo for teaching and learning in medical education. RESULTS: The use of personal smartphones for teaching and learning among medical community is highly prevalent and increasing day by day. Medical students use the mobile application for online textbooks (70%), medical podcasts (60%), medical calculator (75%), online lecture (50%) and notes taking (45%). Relevant studies conclude that the majority of students use smart phones for education (62.7%), communication (81.7%) and recreation (82.5%). Social media has a great potential in educational setting and provide students a chance to involve, share and express knowledge and information with each other. Facebook, WhatsApp and Edmodo are the commonly used applications having multiple benefits like collaboration, feedback and engagement but negative aspects including addiction, distraction and maintenance of privacy have also been found. CONCLUSION: The review article concludes that social media is a powerful instrument for social interactions and is also used as a tool for teaching and learning. The integration of social media with traditional class teaching in medical education has clear advantages but there is a debate about the probable disadvantages as well.