RESUMEN
Ganoderma lucidum, widely used in traditional medicine, has several biological properties. Polysaccharides, mainly glucans, are known as one of its main bioactive compounds. Consequently, the achievement and chemical investigation of such molecules are of pharmaceutical interest. Herein, we obtained water-insoluble and water-soluble polysaccharides from G. lucidum by alkaline extraction. Fractionation process yielded three fractions (GLC-1, GLC-2, and GLC-3). All samples showed to be composed mainly of glucans. GLC-1 is a linear (1 â 3)-linked ß-glucan; GLC-2 is a mixture of three different linear polysaccharides: (1 â 3)-ß-glucan, (1 â 3)-α-glucan, and (1 â 4)-α-mannan; while GLC-3 is a branched ß-glucan with a (1 â 4)-linked main chain, which is branched at O-3 or O-6 by (1 â 3)- or (1 â 6)-linked side chains. This research reports the variability of glucans in Ganoderma lucidum fruiting bodies and applicable methodologies to obtain such molecules. These polysaccharides can be further applied in biological studies aiming to investigate how their chemical differences may affect their biological properties.
Asunto(s)
Ascomicetos , Reishi , beta-Glucanos , Glucanos/química , Reishi/química , Polisacáridos/química , beta-Glucanos/química , Cuerpos Fructíferos de los Hongos/química , Agua/análisisRESUMEN
In situ forming hydrogels are promising for biomedical applications, especially in drug delivery. The precursor solution can be injected at the target site, where it undergoes a sol-gel transition to afford a hydrogel. In this sense, the most significant characteristic of these hydrogels is fast gelation behavior after injection. This study describes an all-polysaccharide, rapidly in situ-forming hydrogel composed of carboxymethyl chitosan (CMCHT) and hydroxyethyl cellulose functionalized with aldehyde groups (HEC-Ald). The HEC-Ald was synthesized through acetal functionalization, followed by acid deprotection. This innovative approach avoids cleavage of pyran rings, as is inherent in the periodate oxidation approach, which is the most common method currently employed for adding aldehyde groups to polysaccharides. The resulting hydrogel exhibited fast stress relaxation, self-healing properties, and pH sensitivity, which allowed it to control the release of an encapsulated model drug in response to the medium pH. Based on the collected data, the HEC-Ald/CMCHT hydrogels show promise as pH-sensitive drug carriers.
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Aldehídos , Celulosa , Celulosa/análogos & derivados , Quitosano , Quitosano/análogos & derivados , Hidrogeles , Quitosano/química , Concentración de Iones de Hidrógeno , Celulosa/química , Hidrogeles/química , Aldehídos/química , Portadores de Fármacos/química , Liberación de Fármacos , Polisacáridos/químicaRESUMEN
Mushroom polysaccharides consist of a unique set of polymers that arrive intact in the human large intestine becoming available for fermentation by resident gut bacteria with potential benefits to the host. Here we have obtained four glucans from two mushrooms (Pholiota nameko and Pleurotus pulmonarius) under different extraction conditions and their fermentation profile by human gut bacteria in vitro was evaluated. These glucans were isolated and characterized as (1 â 3),(1 â 6)-ß-D-glucans varying in branching pattern and water-solubility. An aliquot of each (1 â 3),(1 â 6)-ß-D-glucan was subjected to controlled smith degradation process in order to obtain a linear (1 â 3)-ß-D-glucan from each fraction. The four ß-D-glucans demonstrated different water solubilities and molar mass ranging from 2.2 × 105 g.mol-1 to 1.9 × 106 g.mol-1. In vitro fermentation of the glucans by human gut microbiota showed they induced different short chain fatty acid production (52.0-97.0 mM/50 mg carbohydrates), but an overall consistent high propionate amount (28.5-30.3 % of total short chain fatty acids produced). All glucans promoted Bacteroides uniformis, whereas Anaerostipes sp. and Bacteroides ovatus promotion was strongly driven by the ß-D-glucans solubility and/or branching pattern, highlighting the importance of ß-D-glucan discrete structures to their fermentation by the human gut microbiota.
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Microbioma Gastrointestinal , beta-Glucanos , Humanos , Glucanos/química , beta-Glucanos/metabolismo , Fermentación , Ácidos Grasos Volátiles , AguaRESUMEN
An optimized PLE method was applied to several truffle species using three different solvent mixtures to obtain bioactive enriched fractions. The pressurized water extracts contained mainly (1 â 3),(1 â 6)-ß-D-glucans, chitins, and heteropolymers with galactose and mannose in their structures. The ethanol extracts included fatty acids and fungal sterols and others such as brassicasterol and stigmasterol, depending on the species. They also showed a different fatty acid lipid profile depending on the solvent utilized and species considered. Ethanol:water extracts showed interesting lipids and many phenolic compounds; however, no synergic extraction of compounds was noticed. Some of the truffle extracts were able to inhibit enzymes related to type 2 diabetes; pressurized water extracts mainly inhibited the α-amylase enzyme, while ethanolic extracts were more able to inhibit α-glucosidase. Tuber brumale var. moschatum and T. aestivum var. uncinatum extracts showed an IC50 of 29.22 mg/mL towards α-amylase and 7.93 mg/mL towards α-glucosidase. Thus, use of the PLE method allows o bioactive enriched fractions to be obtained from truffles with antidiabetic properties.
RESUMEN
C. brachyspora, a widespread dematiaceous fungus, was evaluated in this study to optimize the production of exopolysaccharides (CB-EPS). Optimization was performed using response surface methodology, and the best production yielded 75.05% of total sugar at pH 7.4, with 0.1% urea, after 197 h. The obtained CB-EPS showed typical signals of polysaccharides, which was confirmed by FT-IR and NMR. The HPSEC analysis indicated a polydisperse polymer, showing a non-uniform peak, with an average molar mass (Mw) of 24,470 g/mol. The major monosaccharide was glucose (63.9 Mol%), followed by mannose (19.7 Mol%), and galactose (16.4 Mol%). Methylation analysis encountered derivatives that indicated the presence of a ß-d-glucan and a highly branched glucogalactomannan. CB-EPS was tested on murine macrophages to verify its immunoactivity, and the treated cells were able to produce TNF-α, IL-6, and IL-10. However, the cells did not produce superoxide anions or nitric oxide nor stimulated phagocytosis. The results demonstrated an indirect antimicrobial activity of macrophages by stimulating cytokines, showing another biotech applicability for the exopolysaccharides produced by C. brachyspora.
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Macrófagos , Polisacáridos , Animales , Ratones , Espectroscopía Infrarroja por Transformada de Fourier , Polisacáridos/farmacología , BiotecnologíaRESUMEN
Soursop fruits are widely used in the folk medicine to treat a variety of health conditions. Once the chemical structure of dietary fibers from fruits is closely related to its biological functions in the human body, we aimed to explore structural features and biological activity of dietary fibers from soursop. Polysaccharides that constitute the soluble and insoluble fibers were extracted and further analyzed using monosaccharide composition, methylation, molecular weight determination and 13C NMR data. Soursop soluble fibers (SWa fraction) were characterized as having type II arabinogalactan and a highly methyl esterified homogalacturonan, while non-cellulosic insoluble fibers (SSKa fraction) were mainly composed by a pectic arabinan, a xylan-xyloglucan complex and a glucuronoxylan. The oral pre-treatment with SWa and SSKa promoted antinociception in mice writhing test, reducing the number of pain-like behaviors (in 84.2 % and 46.9 %, respectively, at 10 mg/kg) and peritoneal leucocyte migration (55.4 % and 59.1 %, at 10 mg/kg), effects possibly associated with the pectins present in fruit pulp extractions. SWa also significantly inhibited the plasmatic extravasation of Evans blue dye in 39.6 % at 10 mg/kg. This paper describes for the first time the structural features of soursop dietary fibers that may be of biological significance in future.
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Annona , Ratones , Humanos , Animales , Annona/química , Frutas/química , Polisacáridos/química , Antiinflamatorios/farmacología , Antiinflamatorios/análisis , Analgésicos/farmacología , Analgésicos/análisisRESUMEN
A fucoxylomannan (FXM) was isolated from the mushroom Ganoderma lucidum through alkaline extraction followed by dialysis, freeze-thawing, and fractionation by Fehling's solution. The main chain of FXM presented α-d-Manp-(1â4)-linked units, and some of them were branched at O-6 position by α-l-Fucp-(1â2)-ß-d-Xylp groups. Its Mw was 35.9 kDa. FXM was tested on melanoma B16-F10 cells and it showed cell viability and cell density reduction, as well as antiproliferative effect, through cell cycle arrest. Additionally, the anchorage-independent clonogenic capacity of such cells was significantly reduced by FXM, decreasing the number of cells by colony and the colonies area. No effect on viability neither in proliferation of non-tumoral Balb c/3T3 fibroblasts was observed. These results indicate that FXM is a promising anti-proliferative compound impairing pivotal tumorigenic mechanisms, eliciting this polysaccharide to be further explored as an antimelanoma drug.
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Agaricales , Ganoderma , Reishi , Cuerpos Fructíferos de los Hongos , Polisacáridos/farmacología , Diálisis RenalRESUMEN
Melanoma is a highly metastatic and rapidly progressing cancer, a leading cause of mortality among skin cancers. The melanoma microenvironment, formed from the activity of malignant cells on the extracellular matrix and the recruitment of immune cells, plays an active role in the development of drug resistance and tumor recurrence, which are clinical challenges in cancer treatment. These tumoral metabolic processes are affected by proteins, including Galectin-3 (Gal-3), which is extensively involved in cancer development. Previously, we characterized a partially methylated mannogalactan (MG-Pe) with antimelanoma activities. In vivo models of melanoma were used to observe MG-Pe effects in survival, spontaneous, and experimental metastases and in tissue oxidative stress. Analytical assays for the molecular interaction of MG-Pe and Gal-3 were performed using a quartz crystal microbalance, atomic force microscopy, and contact angle tensiometer. MG-Pe exhibits an additive effect when administered together with the chemotherapeutic agent dacarbazine, leading to increased survival of treated mice, metastases reduction, and the modulation of oxidative stress. MG-Pe binds to galectin-3. Furthermore, MG-Pe antitumor effects were substantially reduced in Gal-3/KO mice. Our results showed that the novel Gal-3 ligand, MG-Pe, has both antitumor and antimetastatic effects, alone or in combination with chemotherapy.
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Antineoplásicos , Galectina 3 , Melanoma , Neoplasias Cutáneas , Animales , Antineoplásicos/metabolismo , Antineoplásicos/farmacología , Antineoplásicos/uso terapéutico , Dacarbazina/metabolismo , Dacarbazina/farmacología , Dacarbazina/uso terapéutico , Galectina 3/metabolismo , Galectina 3/farmacología , Galectina 3/uso terapéutico , Ligandos , Melanoma/tratamiento farmacológico , Melanoma/metabolismo , Ratones , Recurrencia Local de Neoplasia , Neoplasias Cutáneas/tratamiento farmacológico , Neoplasias Cutáneas/metabolismo , Microambiente Tumoral/efectos de los fármacos , Microambiente Tumoral/fisiologíaRESUMEN
Polysaccharides α-D-galactan (GAL-Am) and ß-D-glucan (GLC-Am) were obtained from Amanita muscaria fruiting bodies. They were purified using different methodologies, such as Fehling precipitation (for both fractions), freeze-thawing process and ultrafiltration (for GLC-Am). Results showed that the GAL-Am has (1 â 6)-linked Galp main chain branched at O-2 by terminal Galp units and has not been previously reported. Besides, GLC-Am has (1 â 3)-linked Glcp in the main chain, substituted at O-6 by (1 â 6)-linked ß-Glcp units. Both are water-soluble, with 9.0 × 103 g/moL and 1.3 × 105 g/moL, respectively. GAL-Am and GLC-Am presented a selective proliferation reduction against B16-F10 melanoma cell line, not affecting non tumoral BALB/3T3 fibroblast cell line. Furthermore, both fractions reduced clonogenic capacity of melanoma cell line over an extended period of time. These results were obtained without modulations in B16-F10 cell adhesion, reinforcing the biological activities towards cell proliferation impairment and eliciting these polysaccharides as promising compounds to further exploration of their antimelanoma properties.
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Amanita/metabolismo , Antineoplásicos , Galactanos , Glucanos , Melanoma Experimental/tratamiento farmacológico , Animales , Antineoplásicos/química , Antineoplásicos/farmacología , Células 3T3 BALB , Proliferación Celular/efectos de los fármacos , Galactanos/química , Galactanos/farmacología , Glucanos/química , Glucanos/farmacología , RatonesRESUMEN
Most dietary fibers used to shape the gut microbiota present different and unpredictable responses, presumably due to the diverse microbial communities of people. Recently, we proposed that fibers can be classified in a hierarchical way where fibers of high specificity (i.e., structurally complex and utilized by a narrow group of gut bacteria) could have more similar interindividual responses than those of low specificity (i.e., structurally simple and utilized by many gut bacteria). To test this hypothesis, we evaluated microbiota fermentation of fibers tentatively classified as low (fructooligosaccharides), low-to-intermediate (type 2 resistant starch), intermediate (pectin), and high (insoluble ß-1,3-glucan) specificity, utilizing fecal inoculum from distinct subjects, regarding interindividual similarity/dissimilarity in fiber responses. Individual shifts in target bacteria (as determined by linear discriminant analysis) confirmed that divergent fiber responses occur when utilizing both of the low-specificity dietary fibers, but fibers of intermediate and high specificity lead to more similar responses across subjects in support of targeted bacteria. The high-specificity insoluble ß-glucan promoted a large increase of the target bacteria (from 0.3 to 16.5% average for Anaerostipes sp. and 2.5 to 17.9% average for Bacteroides uniformis), which were associated with increases in ratios of related metabolites (butyrate and propionate, respectively) in every microbial community in which these bacteria were present. Also, high-specificity dietary fibers promoted more dramatic changes in microbial community structure than low-specificity ones relative to the initial microbial communities. IMPORTANCE In the face of interindividual variability and complexity of gut microbial communities, prediction of outcomes from a given fiber utilized by many microbes would require a sophisticated comprehension of all competitive interactions that occur in the gut. Results presented here suggest that high-specificity fibers potentially circumvent the competitive scope in the gut for fiber utilization, providing a promising path to targeted and predictable microbial shifts in different individuals. These findings are the first to indicate that fiber specificity is related to similarity and intensity of response in distinct human gut microbiota communities.
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Bacterias/metabolismo , Fibras de la Dieta/análisis , Fibras de la Dieta/metabolismo , Fermentación , Microbioma Gastrointestinal/fisiología , Adulto , Bacterias/clasificación , Butiratos/metabolismo , Carbohidratos/análisis , Ácidos Grasos Volátiles/metabolismo , Heces/microbiología , Femenino , Voluntarios Sanos , Humanos , MasculinoRESUMEN
Most insoluble dietary fibers are known to be relatively poorly fermented by the human gut microbiota. Here, the potential of microwave (MW) treatment to enhance the susceptibility of insoluble fruit polysaccharides to fermentation by the human gut microbiota was evaluated. Insoluble fruits dietary fibers before (xylan A, xylan T, and arabinan) and after MW (xylan A-MW, xylan T-MW, and arabinan-MW) treatment were fermented using an in vitro fermentation model. Gas production, shifts in pH, and short chain fatty acids (SCFAs) production showed an increase in fermentability of all tested dietary fibers, with an average 4-fold increase in SCFAs production after microwaving with total SCFAs ranging from 17.1 mM in the arabinan-MW to 40.4 mM in the xylan T-MW. While arabinan-MW and xylan T-MW promoted all three SCFAs proportionally (acetate:propionate:butyrate), xylan A-MW led to a marked and slow increase in butyrate reaching 28.1% of total SCFAs at 24 h. Rearrangements in three-dimensional structure that potentially facilitate bacterial accessibility to the dietary fiber were observed by scanning electron microscopy in xylan A-MW, forming coin-like particles with ~1.1 µm diameter. 16S rRNA gene sequencing indicated that microbiota shifts were related to both treatment (native versus MW) and dietary fiber type with many butyrogenic species being promoted by xylan A-MW. Overall, MW treatment enhanced insoluble dietary fiber fermentability promoting increased SCFAs production and bacterial shifts which are related to health benefits.
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Microbioma Gastrointestinal , Fibras de la Dieta , Ácidos Grasos Volátiles , Humanos , Microondas , ARN Ribosómico 16SRESUMEN
Mushroom d-glucans are recognized as dietary fibers and as biologically active natural polysaccharides, with the advantages of being quite inexpensive for production, tolerable, and having a range of possible structures and physicochemical properties. The prebiotic potential of mushroom d-glucans has been explored in recent years, but the relationship between their various structural features and activity is poorly understood. This review focuses on comprehensively evaluating the prebiotic potential of mushroom d-glucans in face of their structural variations. Overall, mushroom d-glucans provide a unique set of different structures and physicochemical properties with prebiotic potential, where linkage type and solubility degree seem to be associated with prebiotic activity outcomes. The understanding of the effects of distinct structures and physicochemical properties in mushroom d-glucans on the gut microbiota contributes to the design and selection of new prebiotics in a more predictable way.
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Agaricales/química , Glucanos/química , Glucanos/farmacología , Prebióticos , Animales , Fibras de la Dieta/farmacología , Microbioma Gastrointestinal/efectos de los fármacos , Humanos , Ratones , Polisacáridos/farmacología , SolubilidadRESUMEN
Brazilian cacti have the high potential to be used as a source of mucilages with different technological functions that can be widely explored. The physicochemical, structural and technological properties of mucilages extracted from seven cladodes of cacti native from the semi-arid region of the Brazil were investigated. The mucilages exhibited high yields (8.9 to 21.54%), high contents of carbohydrates (39.77 to 87.68%), proteins (4.27 to 14.76%), and minerals, specifically Ca (2.90 to 15.65%). All species evaluated presented a diverse monosaccharide composition, mainly constituted of galactose and arabinose, suggesting the presence of arabinogalactans as the main polysaccharides. Both showed peaks and bands characteristic of polysaccharides with semi-crystalline structure (14.50-38.60%), due to the high concentration of calcium. From the results, mucilages functional properties showed good water and oil retention capacities and a high emulsification capacity (100%) with stability above 83%. Foaming capacity was above 58% with stability greater than 95%. This study highlights the potential of using cacti as a source of functional mucilages for use in food applications.
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Biodiversidad , Cactaceae/química , Fenómenos Químicos , Mucílago de Planta/química , Brasil , Cactaceae/clasificación , Industria de Alimentos , Minerales/análisis , Proteínas de Plantas/análisis , Polisacáridos/análisisRESUMEN
In the present work, gold nanoparticles were synthesized through a green route by using, for the first time, polysaccharides extracted from pineapple gum (PG) as the reducing and capping agent. The obtained nanoparticles (AuNPs-PG) were characterized by UV-VIS, FTIR, TEM, FESEM, EDX, XRD, and zeta potential measurements, which confirmed that PG was effective to produce AuNPs with an average diameter of 10.3 ± 1.6 nm. The AuNPs-PG were employed as the modifier of glassy carbon paste electrodes (CPE/AuNPs-PG), which were applied as sensitive electrochemical sensors to the determination of the antihistamine drug promethazine hydrochloride (PMZ). Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) measurements showed that the AuNPs-PG could enhance the electronic transfer properties of the glassy carbon paste, which was due to their large surface area and high electrical conductivity. After optimization of the instrumental parameters of square wave voltammetry (SWV) through a Box-Behnken factorial design, a linear relationship between the anodic peak current and PMZ concentration was obtained in the range from 2.0 to 15.7 µmol L-1 in McIlvaine buffer solution pH 5.0. The detection and quantification limits were found to be equal to 1.33 and 4.44 µmol L-1, respectively. The developed sensors could successfully quantify PMZ in different commercial pharmaceutical formulations, with satisfactory levels of accuracy and precision. In addition to improving the analytical features of the electrodes, hemocompatibility assays carried out on erythrocytes and leukocytes showed that the AuNPs-PG do not exhibit toxic effects on the referred cells. This interesting behavior enables their use in biocompatible electrochemical sensing platforms as well as for future biomedical investigations.
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Ananas , Técnicas Biosensibles , Nanopartículas del Metal , Técnicas Electroquímicas , Electrodos , Oro , Límite de Detección , PolisacáridosRESUMEN
Polysaccharides from P. eryngii mushroom were selectively extracted using low-cost technologies (water at different conditions of temperature and pressure). Mannogalactan was the main polysaccharide in cold-water extracted fraction (CWEF), while a linear (1â6)-ß-d-glucan was the main polymer in hot-water extracted fraction (HWEF). Autoclave-extracted fraction (AEF) contained a mixture of at least four different α- and ß-glucans. The report of linear (1â6)-ß-glucan and linear (1â3)-ß-glucan is a new finding for P. eryngii fruiting bodies. The immunostimulatory properties of the fractions on THP-1 macrophages were studied. All fractions at 50, 250 and 500 µg/mL were not cytotoxic and produced different stimulus on NO, IL-1ß and IL-10 secretion by the cells. Thus, our results showed that it is possible to concentrate different P. eryngii polysaccharides in selected fractions using a simple and low-cost procedure. Since biological effects depends on the polysaccharide structure, this technique allows the obtainment of fractions with distinct immunomodulatory activities.
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Cuerpos Fructíferos de los Hongos/química , Polisacáridos Fúngicos/farmacología , Factores Inmunológicos , Macrófagos/efectos de los fármacos , Pleurotus/química , Polisacáridos , beta-Glucanos , Mezclas Complejas/química , Mezclas Complejas/aislamiento & purificación , Mezclas Complejas/farmacología , Polisacáridos Fúngicos/aislamiento & purificación , Humanos , Factores Inmunológicos/aislamiento & purificación , Factores Inmunológicos/farmacología , Inmunomodulación , Estructura Molecular , Polisacáridos/aislamiento & purificación , Polisacáridos/farmacología , Relación Estructura-Actividad , Células THP-1 , beta-Glucanos/aislamiento & purificación , beta-Glucanos/farmacologíaRESUMEN
Baccharis trimera is a native medicinal plant from South America popularly known as "carqueja". Its infusion is traditionally ingested for the treatment and prevention of hepatic disorders. Up to now, only the crude aqueous extract or hydroethanolic fractions, containing the secondary metabolites, have been studied and correlated with their biological action on the liver. Here we report that an inulin type fructan is present in the B. trimera infusion and contributes to the hepatoprotective effect of the species. In vitro, inulin at 300 µg/mL, was able to scavenger 97% of the DPPH radicals. In vivo experiments showed that it protected the liver against CCl4-induced injuries. The administration of inulin at low dose of 1 mg/kg significantly reduced the blood levels of ALT, AST and ALP, reduced the lipid peroxidation and increased the catalase activity and the levels of reduced glutathione in the liver of CCl4-treated mice. Moreover, the administration of inulin at 100 mg/kg increased GSH levels in the liver of Naïve mice. No signs of toxicity were observed. Thus, inulin present in B. trimera infusion protects the liver from the oxidative stress caused by CCl4 administration and can corroborate with the hepatoprotective effects presented by the species infusion.
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Baccharis , Animales , Hígado , Ratones , Extractos Vegetales/farmacología , Polisacáridos/farmacología , América del SurRESUMEN
Chamomile is one of the most ancient medicinal herbs known to mankind and among its traditional uses are the calming effects. However, few studies explored its effects on the central nervous system (CNS). In this study we further proceed with structural elucidation of polysaccharides from chamomile tea. A highly substituted 4-O-methyl-glucuronoxylan (fraction SN-50R) was purified and chemically characterized, presenting Xyl:GlcA ratio of 1.7:1, Mw of 500 kDa and total sugar content of 98%. Its bioactivity on pain and on CNS was explored. Animals treated with SN-50R presented antinociceptive effect and a dose-dependent decrease in the number of crossings in the activity chamber and in the open field test, as well as a significant reduction in the number of marbles buried when compared to control. These results suggest that SN-50R presented sedative and anxiolytic-like effects and may be contributing for the calming effects obtained by chamomile tea ingestion.
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Analgésicos/farmacología , Ansiolíticos/farmacología , Manzanilla/química , Hipnóticos y Sedantes/farmacología , Extractos Vegetales/farmacología , Té/química , Xilanos/farmacología , Animales , Femenino , Masculino , Ratones , Plantas Medicinales/química , Polisacáridos/farmacologíaRESUMEN
Passion fruit peel (PFP) is a by-product from the fruit processing industry, accounting for approximately 50 % of the fruit weight. It is well known for its health properties, although few studies evaluated its rheological properties. PFP polysaccharides (PFPP) contain a high methoxyl pectin (HMP), specifically a 70 % methyl-esterified homogalacturonan. Flow behaviour analysis of PFPP (with or without sucrose) revealed a shear-thinning non-Newtonian behaviour. Dynamic oscillatory tests showed a weak gel-like behaviour, even without sucrose addition. Moreover, under simulated pasteurization process PFPP maintained its gel structure. Taken together we demonstrated that PFPP has divergent behaviour from commercial HMP, since it does not require sucrose or low pH to form gel. The present work reinforces the use of PFP as a source of soluble dietary fibres and pectins, providing its alternative application as a rheological modifier in a wide range of products, including those with low sugar.
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Fibras de la Dieta/análisis , Frutas/química , Passiflora/química , Pectinas/química , Polisacáridos/química , Módulo de Elasticidad , Geles , Humanos , Concentración de Iones de Hidrógeno , Pasteurización , Pectinas/aislamiento & purificación , Transición de Fase , Polisacáridos/aislamiento & purificación , Resistencia al Corte , Residuos/análisisRESUMEN
Exotic fruits and their co-products may be valuable sources of antioxidant dietary fibres (DF) which are useful for food industry and human health. In this study, we aimed to characterize DF obtained from guavira fruit pomace and investigate its antioxidant potential employing TEAC assay as well as a cell model. The DF were chemically characterized as containing arabinan, highly-methoxylated homogalacturonan and arabinogalactan. The DF-containing fraction (CPW) presented ABTS free radical scavenger activity. MTT and DCFH-DA assay were performed to assess, respectively, changes in cell viability and the potential intracellular antioxidant activity against H2O2-induced oxidative stress in murine NIH 3T3 fibroblast. CPW exhibited no effects on cell viability, moreover, when administered 48 h prior the induction of H2O2 toxic effects, it protected the cells, significantly increasing the cell viability compared to control. This protection may be related to the observed reduction of reactive oxygen species levels. Thus, the pre-treatment of cells with guavira DF for 48 h remarkably induced a cytoprotection against pro-oxidant conditions, and may be a valuable functional compound recovered from an unexploited agroindutrial waste.
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Antioxidantes/análisis , Antioxidantes/farmacología , Fibras de la Dieta/análisis , Frutas/química , Animales , Supervivencia Celular/efectos de los fármacos , Fibroblastos/efectos de los fármacos , Galactanos/química , Peróxido de Hidrógeno/toxicidad , Ratones , Células 3T3 NIH , Estrés Oxidativo , Pectinas/química , Polisacáridos/químicaRESUMEN
Truffles, besides the appreciated aromatic compounds, contain other molecules with interesting bioactive properties. A screening of fungal sterols and ß-glucans within different truffle species and locations was carried out. These compounds were extracted with pressurized liquids (PLE) generating enriched fractions. Extraction efficiency was studied with a full-factorial experimental design (Response surface methodology, RSM), using water and ethanol as extraction solvents. Polysaccharides from truffle powder (TP) and the optimal PLE extract (EP) obtained were precipitated and analysed by NMR and GC-MS. THP-1 cell cultures were utilized to test immunomodulatory properties. With the optimal PLE conditions (16.7 MPa, 180 °C, 30 min) 64 and 22.5% yields were obtained respect, with water and ethanol, generating fractions containing respect, 9.1% ß-glucan and 4.5% ergosterol. NMR analyses detected (1 â 3)-ß-glucan structures in truffle. The EP induced a reduction of 40% IL-1ß and 60% IL-6 pro-inflammatory cytokines secretion suggesting potential immunomodulatory activity.