RESUMEN
Contractile rings are formed from cytoskeletal filaments during cell division. Ring formation is induced by specific crosslinkers, while contraction is typically associated with motor protein activity. Here, we engineer DNA nanotubes and peptide-functionalized starPEG constructs as synthetic crosslinkers to mimic this process. The crosslinker induces bundling of ten to hundred DNA nanotubes into closed micron-scale rings in a one-pot self-assembly process yielding several thousand rings per microliter. Molecular dynamics simulations reproduce the detailed architectural properties of the DNA rings observed in electron microscopy. Theory and simulations predict DNA ring contraction - without motor proteins - providing mechanistic insights into the parameter space relevant for efficient nanotube sliding. In agreement between simulation and experiment, we obtain ring contraction to less than half of the initial ring diameter. DNA-based contractile rings hold promise for an artificial division machinery or contractile muscle-like materials.
Asunto(s)
Nanotubos , Proteínas , División Celular , Proteínas/metabolismo , Citoesqueleto de Actina/metabolismo , Miosinas/metabolismo , ADN/metabolismoRESUMEN
We study the outflow dynamics and clogging phenomena of mixtures of soft, elastic low-friction spherical grains and hard frictional spheres of similar size in a quasi-two-dimensional (2D) silo with narrow orifice at the bottom. Previous work has demonstrated the crucial influence of elasticity and friction on silo discharge. We show that the addition of small amounts, even as low as 5%, of hard grains to an ensemble of soft, low-friction grains already has significant consequences. The mixtures allow a direct comparison of the probabilities of the different types of particles to clog the orifice. We analyze these probabilities for the hard, frictional and the soft, slippery grains on the basis of their participation in the blocking arches, and compare outflow velocities and durations of non-permanent clogs for different compositions of the mixtures. Experimental results are compared with numerical simulations. The latter strongly suggest a significant influence of the inter-species particle friction.