RESUMEN
PURPOSE: Gastrectomy is a well-known risk factor for tuberculosis (TB). However, little data are available regarding the relationship between gastrectomy and the risk of nontuberculous mycobacterial (NTM) disease. Here, we investigated the incidence of TB and NTM lung disease in gastrectomized patients. METHOD: New cases of TB and NTM lung infection or disease were examined among patients who had undergone gastrectomy due to gastric cancer from 2003 to 2009 at a tertiary referral hospital in South Korea. Annualized incidence rates for cases were compared with those of the general population. RESULTS: This study included a total of 2,684 patients. New mycobacterial cases were found in 41 patients. Cases of TB and NTM lung infection were 35 (85 %) and 6 (15 %) including 2 NTM lung disease cases, respectively. Annualized crude incidence rates for TB, NTM lung infection, and NTM lung disease were 327/100,000, 56/100,000, and 19/100,000, respectively. The age-standardized incidence rate of TB was significantly higher in gastrectomized patients than in the general population. However, the standardized incidences of NTM lung infection and disease were not significantly different from those of non-gastrectomized patients. Patients with NTM lung infection frequently exhibited comorbid chronic lung disease, while those with TB were more frequently found to have fibronodular lesions on preoperative chest radiography. CONCLUSIONS: Gastrectomy does not appear to increase the risk of NTM lung disease. However, NTM lung infection or disease should be considered as a differential diagnosis of pulmonary TB in gastrectomized patients accompanying chronic lung disease.
Asunto(s)
Gastrectomía/efectos adversos , Infecciones por Mycobacterium no Tuberculosas/epidemiología , Tuberculosis Pulmonar/epidemiología , Adulto , Anciano , Femenino , Humanos , Incidencia , Masculino , Persona de Mediana Edad , República de Corea/epidemiología , Neoplasias Gástricas/cirugía , Centros de Atención TerciariaRESUMEN
The alpha subunit of a prototypical heterotrimeric GTP-binding protein (G protein), which is encoded by a single gene (GPA1) in Arabidopsis, is a modulator of plant cell proliferation. gpa1 null mutants have reduced cell division in aerial tissues throughout development. Inducible overexpression of GPA1 in Arabidopsis confers inducible ectopic cell division. GPA1 overexpression in synchronized BY-2 cells causes premature advance of the nuclear cycle and the premature appearance of a division wall. Results from loss of function and ectopic expression and activation of GPA1 indicate that this subunit is a positive modulator of cell division in plants.
Asunto(s)
Proteínas de Arabidopsis , Arabidopsis/citología , Arabidopsis/metabolismo , Subunidades alfa de la Proteína de Unión al GTP , Proteínas de Unión al GTP Heterotriméricas/metabolismo , Ácido 2,4-Diclorofenoxiacético/farmacología , Alelos , Arabidopsis/genética , Arabidopsis/crecimiento & desarrollo , Tamaño de la Célula , Genes de Plantas , Genes Reporteros , Glucuronidasa/análisis , Glucuronidasa/genética , Guanosina Trifosfato/metabolismo , Proteínas de Unión al GTP Heterotriméricas/química , Proteínas de Unión al GTP Heterotriméricas/genética , Ácidos Indolacéticos/farmacología , Péptidos y Proteínas de Señalización Intercelular , Luz , Sistema de Señalización de MAP Quinasas , Morfogénesis , Mutación , Péptidos/farmacología , Fenotipo , Hojas de la Planta/citología , Hojas de la Planta/crecimiento & desarrollo , Plantas Tóxicas , Subunidades de Proteína , Proteínas Recombinantes/metabolismo , Transducción de Señal , NicotianaRESUMEN
Terminal differentiation of many vascular cells involves cell wall changes. Cells first elongate their primary wall, then lay down a lignified secondary wall, which is often followed by digestion of the primary wall. Expansins are wall proteins that regulate wall changes, but little is known about the specific functions of the many individual expansin isoforms. An in vitro cell culture of synchronously differentiating tracheary elements was used to identify three new expansins and to compare their expression kinetics with the timing of wall changes. The genes encoding these expansins from zinnia (Zinnia elegans), designated ZeExp1, ZeExp2, ZeExp3, are expressed during cell elongation. ZeExp1 and ZeExp2 mRNA decrease at the early stage of secondary wall formation, whereas ZeExp3 does not. In planta, all three ZeExp mRNAs are found predominantly in a single flank of cells adjacent to protoxylem and metaxylem vessels and in cells roughly at the radial position of the fasicular and interfasicular cambium. Furthermore, within these cells, Exp mRNA is localized exclusively either to the apical or basipetal end of cells depending on the expansin gene and organ, providing the first evidence for polar localization of mRNA in plant cells. ZeExp1 and ZeExp3 mRNA are localized at the apical tip, whereas ZeExp2 mRNA is found in the basal tip. These observations indicate that these three expansins are xylem cell specific and possibly involved in the intrusive growth of the primary walls of differentiating xylem cells.
Asunto(s)
Proteínas de Plantas/metabolismo , Plantas/metabolismo , ARN Mensajero/metabolismo , Fracciones Subcelulares/metabolismo , Secuencia de Aminoácidos , Datos de Secuencia Molecular , Células Vegetales , Proteínas de Plantas/química , Plantas/genética , ARN Mensajero/genética , Homología de Secuencia de AminoácidoRESUMEN
To test the hypothesis that auxin-binding protein 1 (ABP1) is a receptor controlling auxin-mediated plant cell expansion, ABP1 complementary DNAs were expressed in a controllable fashion in tobacco plants and constitutively in maize cell lines. Induction of Arabidopsis ABP1 expression in tobacco leaf strips resulted in an increased capacity for auxin-mediated cell expansion, whereas induction of ABP1 in intact plants resulted in leaves with a normal morphology, but larger cells. Similarly, constitutive expression of maize ABP1 in maize cell lines conferred on them the capacity to respond to auxin by increasing cell size. These results support a role of ABP1 as an auxin receptor controlling plant growth.
Asunto(s)
Ácidos Indolacéticos/metabolismo , Reguladores del Crecimiento de las Plantas , Hojas de la Planta/citología , Proteínas de Plantas , Receptores de Superficie Celular/genética , Receptores de Superficie Celular/fisiología , Línea Celular , Tamaño de la Célula , Regulación de la Expresión Génica de las Plantas , Genes de Plantas , Ácidos Indolacéticos/farmacología , Fenotipo , Hojas de la Planta/crecimiento & desarrollo , Hojas de la Planta/metabolismo , Plantas Modificadas Genéticamente , Plantas Tóxicas , Tetraciclinas/farmacología , Nicotiana/citología , Nicotiana/metabolismo , Transformación Genética , Transgenes , Zea mays/citología , Zea mays/metabolismoRESUMEN
Immunohistological analyses for sucrose phosphate synthase (SPS) show that the protein is localized in both bundle-sheath cells (BS) and mesophyll cells (M) in maize (Zea mays) leaves. In young leaves, SPS protein was predominantly in the BS, whereas mature leaves showed nearly equal levels of signal in both BS and M. A cell-type-specific response was also seen in light and dark treatments. Dark treatments led to reduced signal in M; however, little or no change was detected in BS. We suggest that SPS in BS is engaged in sucrose biosynthesis by both photoassimilatory and starch turnover reactions in maize leaves. In addition, we suggest that the enzyme in BS may play a major role in the early biosynthesis of sucrose in young leaves. These cell-specific changes in expression in situ were in agreement with the estimates of extractable enzyme activity from isolated BS and M of mature leaves (R. Ohsugi, S.C. Huber [1987] Plant Physiol 84: 1096-1101). In contrast, western blot analyses did not show any significant changes in the levels of SPS protein in either young or mature leaves subsequent to similar dark treatments. It is interesting that the northern blot analyses indicate that the steady-state levels of SPS transcripts were markedly reduced after dark treatments of > 12 h. Overall, our results indicate that Sps gene expression in maize leaf is modulated at multiple levels of controls by both developmental and environmental factors.