Synergistic Reinforcement of Composite Hydrogels with Nanofiber Mixtures of Cellulose Nanocrystals and Chitin Nanofibers.

Simultaneous incorporation of cellulose nanocrystals (CNCs) and chitin nanofibers (ChNFs) into a polyvinyl alcohol (PVA) matrix opens possibilities for customization of more environmentally friendly composite materials. When used in tricomponent composite hydrogels, the opposite surface charges on CNCs and ChNFs lead to the construction of beneficial nanofiber structures. In this work, composite hydrogels containing CNCs, ChNFs, or their mixtures are produced using cyclic freeze-thaw (FT) treatments. When considering different compositions and FT cycling, tricomponent composite hydrogels containing a specific ratio of CNCs/ChNFs are shown to have promising mechanical performance in comparison to other samples. These results together with results from water absorption, rheological, and light scattering studies suggest that the CNC/ChNF structures produced property improvement by concurrently accessing the stronger interfacial interactions between CNCs and PVA and the longer lengths of the ChNFs for load transfer. Overall, these results provide insight into using electrostatically driven nanofiber structures in nanocomposites.

Controlling Barrier and Mechanical Properties of Cellulose Nanocrystals by Blending with Chitin Nanofibers.

Chitin nanofibers (ChNFs) and cellulose nanocrystals (CNCs) have been proposed as materials for renewable packaging with low O2 transmission that protect food, medicine, and electronics. A challenge in biomass-derived functional materials is tuning both barrier and mechanical properties, while minimizing process steps. A concept that merits additional study in this field is tuning of the barrier and mechanical properties by use of oppositely charged biomass-derived fibers, through interactions that support dense film formation. We report free-standing films formed by solution casting of blends of aqueous suspensions of CNCs and ChNFs with either low degree of acetylation (LChNFs, higher charge) or high degree of acetylation (HChNFs, lower charge). While neat CNC films had the highest O2 permeability (OP), the OP was lowered by 91% by addition of at least 25 wt % LChNFs to CNCs to an OP value near 1.7 cm3 µm/m2/d/kPa. Interestingly, blends of CNCs with less highly charged, larger HChNFs had equivalently lower OP as with LChNFs. The tensile strength and strain at break of blended ChNF/CNC films was optimal compared to neat cellulose or chitin when at least 50 wt % LChNFs or HChNFs were blended with CNCs. We show that the ability to tune properties of ChNF/CNC blends was coincident with the formation of aggregates of chitin and cellulose nanomaterials, which appear to support formation of dense layers of tortuous fiber networks.

The effect of surface topography and porosity on the tensile fatigue of 3D printed Ti-6Al-4V fabricated by selective laser melting.

Additive manufacturing (3D printing) is emerging as a key manufacturing technique in medical devices. Selective laser melted (SLM) Ti-6Al-4V implants with interconnected porosity have become widespread in orthopedic applications where porous structures encourage bony ingrowth and the stiffness of the implant can be tuned to reduce stress shielding. The SLM technique allows high resolution control over design, including the ability to introduce porosity with spatial variations in pore size, shape, and connectivity. This study investigates the effect of construct design and surface treatment on tensile fatigue behavior of 3D printed Ti-6Al-4V. Samples were designed as solid, solid with an additional surface porous layer, or fully porous, while surface treatments included commercially available rotopolishing and SILC cleaning. All groups were evaluated for surface roughness and tested in tension to failure under monotonic and cyclic loading profiles. Surface treatments were shown to reduce surface roughness for all sample geometries. However, only fatigue behavior of solid samples was improved for treated as compared to non-treated surfaces Irrespective of surface treatment and resulting surface roughness, the fatigue strength of 3D printed samples containing bulk or surface porosity was approximately 10% of the ultimate tensile strength of identical 3D printed porous material. This study highlights the relative effect of surface treatment in solid and porous printed samples and the inherent decrease in fatigue properties of 3D printed porous samples designed for osseointegration.

Impact of surface porosity and topography on the mechanical behavior of high strength biomedical polymers.

The ability to control the surface topography of orthopedic implant materials is desired to improve osseointegration but is often at the expense of mechanical performance in load bearing environments. Here we investigate the effects of surface modifications, roughness and porosity, on the mechanical properties of a set of polymers with diverse chemistry and structure. Both roughness and surface porosity resulted in samples with lower strength, failure strain and fatigue life due to stress concentrations at the surface; however, the decrease in ductility and fatigue strength were greater than the decrease in monotonic strength. The fatigue properties of the injection molded polymers did not correlate with yield strength as would be traditionally observed in metals. Rather, the fatigue properties and the capacity to maintain properties with the introduction of surface porosity correlated with the fracture toughness of the polymers. Polymer structure impacted the materials relative capacity to maintain monotonic and cyclic properties in the face of surface texture and porosity. Generally, amorphous polymers with large ratios of upper to lower yield points demonstrated a more significant drop in ductility and fatigue strength with the introduction of porosity compared to crystalline polymers with smaller ratios in their upper to lower yield strength. The latter materials have more effective dissipation mechanisms to minimize the impact of surface porosity on both monotonic and cyclic damage.

Seeking homeostasis: temporal trends in respiration, oxidation, and calcium in SOD1 G93A Amyotrophic Lateral Sclerosis mice.

Impairments in mitochondria, oxidative regulation, and calcium homeostasis have been well documented in numerous Amyotrophic Lateral Sclerosis (ALS) experimental models, especially in the superoxide dismutase 1 glycine 93 to alanine (SOD1 G93A) transgenic mouse. However, the timing of these deficiencies has been debatable. In a systematic review of 45 articles, we examine experimental measurements of cellular respiration, mitochondrial mechanisms, oxidative markers, and calcium regulation. We evaluate the quantitative magnitude and statistical temporal trend of these aggregated assessments in high transgene copy SOD1 G93A mice compared to wild type mice. Analysis of overall trends reveals cellular respiration, intracellular adenosine triphosphate, and corresponding mitochondrial elements (Cox, cytochrome c, complex I, enzyme activity) are depressed for the entire lifespan of the SOD1 G93A mouse. Oxidant markers (H2O2, 8OH2'dG, MDA) are initially similar to wild type but are double that of wild type by the time of symptom onset despite early post-natal elevation of protective heat shock proteins. All aspects of calcium regulation show early disturbances, although a notable and likely compensatory convergence to near wild type levels appears to occur between 40 and 80 days (pre-onset), followed by a post-onset elevation in intracellular calcium. The identified temporal trends and compensatory fluctuations provide evidence that the "cause" of ALS may lay within failed homeostatic regulation, itself, rather than any one particular perturbing event or cellular mechanism. We discuss the vulnerabilities of motoneurons to regulatory instability and possible hypotheses regarding failed regulation and its potential treatment in ALS.

State of the field: An informatics-based systematic review of the SOD1-G93A amyotrophic lateral sclerosis transgenic mouse model.

Numerous sub-cellular through system-level disturbances have been identified in over 1300 articles examining the superoxide dismutase-1 guanine 93 to alanine (SOD1-G93A) transgenic mouse amyotrophic lateral sclerosis (ALS) pathophysiology. Manual assessment of such a broad literature base is daunting. We performed a comprehensive informatics-based systematic review or 'field analysis' to agnostically compute and map the current state of the field. Text mining of recaptured articles was used to quantify published data topic breadth and frequency. We constructed a nine-category pathophysiological function-based ontology to systematically organize and quantify the field's primary data. Results demonstrated that the distribution of primary research belonging to each category is: systemic measures an motor function, 59%; inflammation, 46%; cellular energetics, 37%; proteomics, 31%; neural excitability, 22%; apoptosis, 20%; oxidative stress, 18%; aberrant cellular chemistry, 14%; axonal transport, 10%. We constructed a SOD1-G93A field map that visually illustrates and categorizes the 85% most frequently assessed sub-topics. Finally, we present the literature-cited significance of frequently published terms and uncover thinly investigated areas. In conclusion, most articles individually examine at least two categories, which is indicative of the numerous underlying pathophysiological interrelationships. An essential future path is examination of cross-category pathophysiological interrelationships and their co-correspondence to homeostatic regulation and disease progression.