Immune and microbiological disorders in women with infectious and inflammatory pathology of the cervix.

Penetration into the female genital tract of infectious agents is associated with the cause of the onset of cervicitis. In most cases, these are sexually transmitted diseases (STDs). A high level of viral and bacterial load in the study of clinical material from women with infectious and inflammatory pathology (IVP) of the cervix affects the activity of innate immunity reactions. The total number of examined patients was 76 patients of reproductive age, of which 36 women with cervical IVP and 40 women made up the comparison group. The imbalance of microbiocenosis can be accompanied by immune and microbiological disorders. The aim of the study was to study immune and microbiological disorders in women with infectious and inflammatory pathology of the cervix. To achieve the goal, the following tasks were set: to identify the relationship between the expression level of TLR2 and TLR4 genes in the epithelial cells of the cervical canal of women in which UPM is determined. To determine the species spectrum and etiological significance of the microbiota of bacterial biofilm of the cervical mucosa in the pathogenesis of cervicitis in women of reproductive age. It has been established that the determination of indicators of innate immunity, such as interferons, in blood serum and the expression of TLR2 and TLR4 receptors, with the study of their balance, taking into account the pro-inflammatory and anti-inflammatory properties in IVP of the cervix, has an important prognostic value. An increase in the level of the immune response in the form of hyperstimulation of antigens of IVP pathogens leads to the chronization of the inflammatory process in the urogenital tract, to scarring of tissues, which can play an important role in the development of infertility and termination of pregnancy in women of reproductive age.

Development and study of nutrient media for determining the biological properties of Listeria.

The commercial nutrient media for investigation of the biologic properties of L. monocytogenes is developed. The efficiency of the use of developed media for determination of motility and lecithinase activity of Listeria in the establishment of the isolated culture to the pathogenic species is shown. These nutrient media are developed for visually accurate registration of the motility and lecithinase activity of Listeria when identifying the isolated cultures improving the diagnostics of listeriosis.

[Microbiological study of sanitary feature of Perinatal Center of Makhachkala City].

AIM: Evaluation of bacterial contamination of six hospital environment of Perinatal Center of Makhachkala as part of epidemiologic surveillance for nosocomial infections. MATERIALS AND METHODS: One hundred twenty-eight air samples from different hospital units and 344 swabs from hospital equipment, instruments, and inventory were tested. Dry nutrient media manufactured by Scientific Manufacturing Organization "Pitatelnye Sredy" were used for isolation and identification of microorganisms. Species of microorganisms was determined on the basis of complex of tinctorial, morphological, biochemical, and serologic tests. RESULTS: Significant species diversity of opportunistic microorganisms was established. Cultures of Staphylococcus epidermidis (46; 18.5%) and Staphylococcus saprophyticus (44; 17.7%) were significantly more frequently isolated from swabs from environment. CONCLUSION: Microbiological monitoring of sanitary conditions of perinatal center assists sanitary-epidemiologic control for circulation of microorganisms--potential agents of nosocomial infections.

[Growth media for isolation, accumulation, and identification of Listeria in laboratory diagnostics of mix-infections in women with obstetric-gynecological pathology].

Isolation of Lysteria monocytogenes from clinical specimens in obstetric-gynecological pathology is often difficult owing to the presence of the mixed microbial flora. Results of study of new Russian growth media for isolation of Listeria for microbiological diagnostics of listeriosis in pregnancy are presented. They show diagnostic efficacy of the developed media for laboratory diagnostics of mix-infections in women with obstetric-gynecological pathology.