[Analysis of the cytochrome P450 (CYP1A1) gene polymorphism in the ethnic groups of the Republic of Bashkortostan].

In three ethnic groups from the Republic of Bashkortostan, Russians (N = 451), Tatars (N= 333), and Bashkirs (N= 171), allele, genotype, and haplotype frequency distribution patterns of the CYP1A1 gene single nucleotide polymorphisms, A2455G and T33801C, were investigated. Substantial interethnic differences in the allele frequency distribution patterns of the CYPIA1 polymorphisms A2455G and T3801C (chi2 = 15.61, d.f. = 2, P = 0.0001; and chi2 = 22.10, d.f. = 2, P = 0.0001, respectively) were observed. Pairwise comparison showed that ethnic groups of Tatars and Russians were similar in the A2455G allele frequencies (chi2 = 1.10, d.f. = 1, P = 0.30). However, in case of the T3801C marker, statistically significant differences were revealed (chi2 = 4.56, d.f. = 1, P = 0.032). At the same time, Bashkir ethnic group was found to be statistically significantly different from Russians and Tatars in the CYP1A1 polymorphic allele frequency distribution patterns (chi2 = 15.74, d.f. = 2, P = 0.0001; and chi2 = 7.47, d.f. = 1, P = 0.024, for A2455G, and chi2 = 6.46, d.f. = 1, P = 0.011; and chi2 = 21.36, d.f. = 1, P = 0.0001, for T3801C). Analysis of the CYP1A1 haplotype diversity showed that in terms of the CYP1A1 haplotype frequency distribution patterns, Bashkir ethnic group was statistically significantly different from both Russians (chi2 = 30.07, d.f. = 3, P = 0.0001) and Tatars (chi2 = 11.28, d.f. = 3, P = 0.013). The differences observed were caused by the high frequency of haplotype CYP1A1*2B, which was represented by a combination of rare alleles of the CYP1A1 polymorphisms A2455G and T3801C in Bashkirs (5.81%). On the other hand, the ethnic groups of Russians and Tatars residing in the Republic of Bashkortostan were characterized by similar frequencies of the CYP1A1 haplotypes (chi2 = 6.322, d.f. = 3, P = 0.127). The data obtained could be used in further investigations of the genetic bases of ecology dependant diseases and in the risk groups in the Republic of Bashkortostan.

[A contribution to the nomenclature and classification of chlamydiae].

The results of molecular-genetic study of typical specimens of the genus Chlamydophila of the family Chlamydiaceae from the FSE FCTRSA collection of microorganisms are considered for the purpose of their taxonomy based on comparative analysis by the omp1-, omp2-, 16S rRNA-, and 23S rRNA-genes and by the presence of extrachromosomal plasmid with corresponding fragments of the genomes officially registered species of chlamydiae. According to the omp1-RFLP-AluI-profile, the Rostinovo-70, 250, PP-87, and KC-93 strains were characterized by new genotype of chlamydiae (genotype G). The new omp2-RFLP-AluI-profile of chlamydiae on carrying out the comparative analysis of Rostinovo-70, 250, PP-87 and KC-93 strains by omp2-gene was characterized. On the basis of the species identification of chlamydiae by genetical and ecological criteria, the chlamydia strains Rostinovo-70, 250, PP-87, and KC-93 can be included in the new chlamydia species Chlamydophila parapsittaci sp. nov. It was suggested to combine into a new species the following strains of Chlamydophila psittaci: WC, NJ1, 92-1293, TT3, 7344/2, GD, CT1, Par1, 84/2334, R54, VS225, 777B15, and Prk/Daruma.