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Due to its harmful effects on ecosystems and human health, microplastic (MP) pollution has become a significant environmental problem on a global scale. Although MPs' pollution path and toxic effects on marine habitats have been examined worldwide, the studies are limited to the rare biodiversity estuary region of Hatiya Island from the northern Bay of Bengal. This study aimed to investigate the MP pollution path and its influencing factors in estuarine sediments and water in rare biodiversity Hatiya Island in the northern Bay of Bengal. Sixty water and sediment samples were collected from 10 sampling sites on the Island and analyzed for MPs. The abundance of MPs in sediment ranged from 67 to 143 pieces/kg, while the abundance in water ranged from 24.34 to 59 pieces/m3. The average concentrations of MPs in sediment and water were 110.90 ± 20.62 pieces/kg and 38.77 ± 10.09 pieces/m3, respectively. Most identified MPs from sediment samples were transparent (51%), while about 54.1% of the identified MPs from water samples were colored. The fragment was the most common form of MP in both compartments, with a value of 64.6% in sediment samples and 60.6% in water samples. In sediment and water samples, almost 74% and 80% of MP were <0.5 mm, respectively. Polypropylene (PP) was the most abundant polymer type, accounting for 51% of all identified polymers. The contamination factor, pollution load index, polymer risk score, and pollution risk score values indicated that the study area was moderately polluted with MPs. The spatial distribution patterns and hotspots of MPs echoed profound human pathways. Based on the results, sustainable management strategies and intervention measures were proposed to reduce the pollution level in the ecologically diverse area. This study provides important insights into evaluating estuary ecosystem susceptibility and mitigation policies against persistent MP issues.
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Ecosistema , Contaminantes Químicos del Agua , Humanos , Plásticos , Microplásticos , Bahías , Estuarios , Biodiversidad , Polímeros , Agua , Monitoreo del Ambiente , Sedimentos GeológicosRESUMEN
This study undertakes a spatiotemporal analysis of microplastic pollution in surface beach sediments, covering 7 coastal beaches in Bangladesh and two seasons-monsoon and winter. The concentration of microplastics extracted from the surface beach sediment is 242.86 particles/kg dw. The results showed both significant seasonal (p value = 0.001) and spatial (p value = 0.004) variation. The abundance and polymer types were significantly higher (57 %) in winter than in the monsoon season (43 %). Touristic and commercial beaches showed higher levels of microplastic pollution than the non-touristic beaches. Polyethylene (28.8 %) and Polypropylene (27.6 %) were the most abundant polymer. The most dominant coloration of microplastics was white (42.6 %). The majority of the microplastics were fibers (33.5 %). Smallest particles measuring <1 mm constituted nearly half of the total microplastics load (48.5 %). This baseline data can be useful in terms of coastal zone management for the southeastern coastal beaches of Bangladesh.
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Plastic-based food-contact materials are potentially threatening the environment and public health by releasing toxic heavy metals. This study aimed to identify the types of plastic commonly used in Bangladesh as food-contact materials (FCMs) and assess the migration of heavy metals from these FCMs. Plastic types were identified using attenuated total reflectance fourier transform infrared spectroscopy (ATR-FTIR), and 25 samples were selected based on the category, including Polyethylene Terephthalate (PET), Polyethylene (PE), Polypropylene (PP), Polystyrene (PS), and Polycarbonate (PC). Distilled water, 3% acetic acid, and 15% ethanol were used as food simulants to assess the overall migration of chemicals at 70 °C for 2 h. The concentrations of heavy metals (Pb, Cd, Hg, Cr, and Sb) were analyzed using an Atomic Absorption Spectrophotometer (AAS). Results revealed that the highest overall migration occurred in coffee cups measuring 3.50 ± 0.17 mg/kg (using water simulant) and in yogurt containers with a measurement of 9.17 ± 0.1 mg/kg (using 3% acetic acid). The highest concentrations of Pb, Cd, Hg, Cr, and Sb were found in PP-2 (0.45 ± 0.01 mg/kg), PP-2 (0.36 ± 0.01 mg/kg), PC-5 (0.27 ± 0.01 mg/kg), PET-2 (0.12 ± 0.01 mg/kg), and PET-1 (0.09 ± 0.01 mg/kg), respectively. The concentration of heavy metals migrated from the containers is likely to induce a health risk due to bioaccumulation from long-term ingestion of food packaged in them. The findings of this study added knowledge about harmful heavy metals leached from the FCMs in Bangladesh.
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This study investigated the prevalence of microplastics (MPs) in the gastrointestinal tract (GIT) of fish from the western coast of Bangladesh, the world's largest mangrove ecosystem. Altogether, 8 different species of fish (5 demersal and 3 pelagic) were examined. Microplastics were detected in every individual fish with an average abundance of 7.1 ± 3.14 particles per specimen. The demersal species were observed to consume more microplastics (7.78 ± 3.51) than the pelagic species (5.92 ± 2.06). Moreover, small-sized fish was found to accumulate higher MPs/body weight than large-sized fish. Polypropylene was the most abundant polymer type (45 %) and fiber was the most prevalent shape (71 %). SEM analysis revealed cracks, pits, and foreign particles on the microplastics' surface, representing their ability to bear organic pollutants and heavy metals. This study will be a source of information for future research and a guide for policy-makers to take better actions to protect and restore marine resources.
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Microplásticos , Contaminantes Químicos del Agua , Animales , Plásticos , Ecosistema , Bangladesh , Contaminantes Químicos del Agua/análisis , Monitoreo del Ambiente , Tracto Gastrointestinal/química , PecesRESUMEN
Drinking water treatment plants (DWTPs) are intended to provide safe water to the municipality, typically by treating surface waters from rivers, lakes, and streams. Regrettably, all of these water sources for DWTPs have been reported to be contaminated by microplastics (MPs). Hence, there is an urgent need to investigate the removal efficiencies of MPs from raw waters in the conventional DWTPs anticipating public health concerns. In this experiment, MPs in the raw and treated waters of the three major DWTPs of Bangladesh, having different water treatment processes, were evaluated. The concentrations of MPs in the inlet points of Saidabad Water Treatment Plant phase-1 and 2 (SWTP-1 and SWTP-2), which share a similar water source of the Shitalakshya River, were 25.7 ± 9.8 and 26.01 ± 9.8 items L-1. The third plant, Padma Water Treatment Plant (PWTP) utilizes water from the Padma River and had an initial MP concentration of 6.2 ± 1.6 items L-1. The studied DWTPs, with their existing treatment processes, were found to reduce the MP loads substantially. The final MP concentrations in treated waters of SWTP-1, SWTP-2, and PWTP were 0.3 ± 0.03, 0.4 ± 0.01, and 0.05 ± 0.02 items L-1 with the removal efficiencies of 98.8, 98.5, and 99.2 %, respectively. The considered size range of MP was 20 µm to <5000. Fragments and fibers were the two predominant MP shapes. In terms of polymer, the MPs were polypropylene (PP, 48 %), polyethylene (PE, 35 %), polyethylene terephthalate (PET, 11 %), and polystyrene (PS, 6 %). The field emission scanning electron microscopy-energy dispersive X-ray spectroscopy (FESEM-EDX) revealed the fractured and rough surfaces of the remaining MPs, which were also found to be contaminated with heavy metals, like lead (Pb), cadmium (Cd), chromium (Cr), arsenic (As), copper (Cu), and zinc (Zn). Hence, additional initiatives are required to remove the residual MPs from the treated waters to safeguard the city dwellers from potential hazards.
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Agua Potable , Contaminantes Químicos del Agua , Purificación del Agua , Microplásticos , Plásticos , Bangladesh , Contaminantes Químicos del Agua/análisis , Monitoreo del Ambiente/métodosRESUMEN
In today's world, microplastic contamination of aquatic systems is a subject of major concern. In this study, the incidence of microplastics in freshwater fish from both farmed and wild sources was evaluated. Wild catla (Gibelion catla), stinging catfish (Heteropneustes fossilis), rui (Labeo rohita), tengara catfish (Mystus tengara), and pabdah catfish (Ompok pabda) were collected from Padma River, Turag River, and Bhairab River. Farmed fishes of same species were collected from Mymensingh and Chandpur fish markets. A total of 68 (43.6%) microparticles out of 156 were identified as microplastics using ATR-FTIR. About 66.67% (20 out of 30) of farmed fish samples had microplastics in their digestive system, while 88.4% (23 of 26) of wild fish samples presented microplastics. There was a significant difference (p < 0.05) in average microplastic count between wild and farmed fish. Average microplastic count also showed a significant difference among five fish species (p < 0.01). A variety of polymers were observed, with polyethylene being the most abundant, followed by polyester, polypropylene, polyamide-6, and polyurethane. This study suggests that microplastic contamination is ubiquitous and contamination is common in all of the fish species investigated, independent of their origin (wild or farmed). However, wild fish population is at a greater risk of ingesting microplastics. This study, for the first time ever, reports plastic pollution in freshwater fishes of Bangladesh from wild and farmed sources.
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Bagres , Cyprinidae , Contaminantes Químicos del Agua , Animales , Microplásticos , Plásticos , Bangladesh , Contaminantes Químicos del Agua/análisis , Peces , Agua Dulce , Ríos , Monitoreo del AmbienteRESUMEN
Microplastics (MPs), together with microfibers, have emerged as a contaminant of concern all around the globe. MPs have been detected in freshwater, seawater, sediment, and aquatic species among others. As suggested by several recent investigations, sea salts, a daily intake item by humans, are also contaminated by MPs. The current article describes MPs' occurrence, distribution, type, and timeline variation in raw sea salts from Cox's Bazar, Bangladesh. MPs have been detected in every collected salt sample, and quantity varied from 28.53 ± 2.43 to 93.53 ± 4.21 particles per kg, which was about 52.48 ± 1.72 to 67.46 ± 3.81 µg/kg of raw salt. Microfibers were MPs' dominant shape category, and the plastic types were mainly polyester or nylon. Other types of MPs were polyethylene (PE), polypropylene (PP), polycarbonate (PC), polyurethane (PU), and polystyrene (PS) in decreasing amounts. The majority of the MPs in the sea salts were in the size range of Ë 3-1 mm. The total amount of MPs and plastic-type variation due to sampling location (p Ë 0.05) and because of the time period (p Ë 0.05) was found insignificant. Acetaldehyde, a volatile toxic substance produced by the degradation of polyester polymer chains, was detected in MPs in the range of 0.37 to 1.72 µg/g by headspace GC-MS analysis. Hence, the sea salts contaminated with MPs pose a public health hazard. Microplastics extraction from sea salts and their characterization.
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Microplásticos , Contaminantes Químicos del Agua , Humanos , Microplásticos/análisis , Plásticos/análisis , Sales (Química)/análisis , Bangladesh , Contaminantes Químicos del Agua/análisis , Monitoreo del Ambiente , PoliésteresRESUMEN
Microplastics (MPs) are an emerging environmental problem due to their all-around existence and extraordinary stability. A significant number of studies are found in recent literature on the occurrence, distribution, transport, and fate of the MPs in several environmental compartments. In this study, we have investigated the occurrence and characteristics of MPs in the surface water and sediment of the Buriganga river, located beside the mega-city of Dhaka in Bangladesh. In the Buriganga river, the concentration of MPs in the surface water was found from 4.33 ± 0.58 to 43.67 ± 0.58 items L-1, and in the sediment, MPs varied from 17.33 ± 1.53 to 133.67 ± 5.51 items kg-1 of dry sediment. Fragment-type MPs were predominant in the surface water and sediment, which was 72.7% and 85.5% respectively. The most abundant polymer type polypropylene (PP) was found -to be 46% in the surface water and 61% in the sediment sample. The next major category, polyethylene (PE) was found to be 26% and 21%, respectively. Polystyrene (PS), polyvinyl chloride (PVC), polyethylene terephthalate (PET), and polyamide (PA) were other commonly detected polymer types. The MPs were found to be contaminated by Pb, Cd, Cr, Zn, Cu, and Sn from Energy dispersive-X-ray fluorescence (ED-XRF) analysis. Tannery-induced Cr was detected in the highest concentrations in the MPs, which were 20.67 ± 1.66 mg kg-1 (in surface water) and 14.2 ± 1.25 mg kg-1 (in sediment). The pollution load index (PLI) of the MPs contamination in different sampling sites along the Buriganga river was found in the risk level category of I and II. The anthropogenic influence of the city area was reflected in the PLI values, which had an increasing trend from the upstream sampling points (1.00 ± 1.00, 1.00 ± 1.00) to the downstream sites (10.09 ± 1.00, 7.71 ± 3.60).
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Microplásticos , Contaminantes Químicos del Agua , Bangladesh , Cadmio/análisis , Monitoreo del Ambiente , Sedimentos Geológicos , Plomo/análisis , Nylons , Plásticos , Polietileno/análisis , Tereftalatos Polietilenos , Polipropilenos/análisis , Poliestirenos/análisis , Cloruro de Polivinilo/análisis , Agua/análisis , Contaminantes Químicos del Agua/análisisRESUMEN
PURPOSE: Construction worker health and safety is a primary concern for construction companies and researchers. Arabian Gulf region, like Saudi Arabia, has been experiencing extremely hot and humid (EHH) weather, which directly affects construction workers' health and safety. This study aims to address the problem of EHH weather conditions and their impacts on construction workers' physiological status. METHODS: This study assesses the impacts of EHH weather on construction workers' physiological status through the measurement of workers' physical body parameters (age, height, and weight); type of activities; and assigned tasks. Thirty-five multinational workers participated in the measurements, which were conducted in real construction site conditions A quantitative analysis was then applied to quantify the physiological impacts of the weather conditions. Several hypotheses were tested to identify the significant impacts of individual and working aspects on the workers' physiological responses. RESULTS AND CONCLUSION: The results provide empirical evidence that the recorded Heart Rate (HR) exceeded the acceptable physiological zones for construction workers exposed to extremely hot and humid weather conditions. Physical body parameters, work activities, and worker status significantly influence construction workers' physiological responses. This study recommends adopting a continuous monitoring approach as an early warning system under extremely hot and humid weather conditions.
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Industria de la Construcción , Salud Laboral , Calor , Humanos , Tiempo (Meteorología) , Lugar de TrabajoRESUMEN
Flutamide which is used to treat prostate cancer and other diseases induces liver damage during and after the therapy. The aim of this study was to develop a flutamide/piperineco-loaded self-emulsifying drug delivery system (FPSEDDS) to inhibit flutamide-induced liver injury by utilizing piperine as a metabolic inhibitor. The development of SEDDS was carried out following a quality by design (QbD) approach. The risk assessment study was performed to identify critical quality attributes (CQAs) and critical material attributes (CMAs)/critical process parameters (CPPs). I-optimal mixture design was executed with three CMAs as the independent variables and CQAs as the dependable variables. The effectiveness of optimized SEDDS to circumvent flutamide-induced hepatotoxicity was assessed in mice. The numerical optimization suggested an optimal formulation with a desirability value of 0.621, using CQAs targets as optimization goals with 95% prediction intervals (α = 0.05). The optimal formulation exhibited the grade A SEDDS characteristics with the guarantee of high payloads in self-formed oily droplets. The design space was also obtained from the same optimization goals. All CQA responses of verification points were found within the 95% prediction intervals of the polynomial models, indicating a good agreement between actual versus predicted responses within the design space. These obtained responses also passed CQAs acceptance criteria. Finally, hematoxylin-eosin staining revealed the minimal flutamide-induced hepatotoxicity from the optimal SEDDS formulation as compared to the control and flutamide/piperine normal suspension. We demonstrate that the piperine containing optimized SEDDS formulation developed by QbD significantly reduces the flutamide-induced liver injury in mice.
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Sistemas de Liberación de Medicamentos , Flutamida , Animales , Emulsiones , Flutamida/toxicidad , Hígado , Masculino , RatonesRESUMEN
The application of surgical suture-thread and the systemic analgesics regimens for pain control in the postoperative surgery remain the criterion standard. However, these medications have daunting adverse effects on the body's innate pain management system. To address this issue, we have developed a local analgesic-loaded suture system which could be efficiently used for surgical repair with localized sedation effect. The drug-loaded conventional suture has modified by adhesive poly-dopamine coating with the local anesthetic lidocaine. The surface modifications have been ascertained by FE-SEM imaging. The tensile strength of suture ensures required elasticity to use in surgical skin closure. In vitro drug release and the in vivo local analgesia was achieved one day after surgery and persisted approximately for one week in 80% of treated animals. Our pre-clinical results suggest that drug-loaded surgical thread may be an effective strategy for improving the overall outcome.
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Sistemas de Liberación de Medicamentos , Liberación de Fármacos , Indoles/administración & dosificación , Lidocaína/administración & dosificación , Manejo del Dolor/métodos , Dolor Postoperatorio/tratamiento farmacológico , Polímeros/administración & dosificación , Suturas/estadística & datos numéricos , Analgesia/métodos , Anestésicos Locales/administración & dosificación , Animales , Masculino , Ratas , Ratas Sprague-Dawley , Resistencia a la TracciónRESUMEN
BACKGROUND: There is a growing need for an evidence-based approach to home support for people with dementia and their carers following diagnosis but research on the effectiveness and cost-effectiveness of different approaches is sparse. The Dementia Early Stage Cognitive Aids New Trial (DESCANT) will evaluate the clinical and cost-effectiveness of a range of memory aids, training and support to people with mild to moderate dementia and their carers at home and compares that intervention with treatment as usual. METHODS/DESIGN: This is a multi-site, pragmatic randomised trial preceded by a feasibility study and internal pilot. We aim to allocate at random 360 pairs comprising a person with mild to moderate dementia and an identified carer between the DESCANT intervention and treatment as usual. We assess participants at baseline, 13 and 26 weeks. The primary outcome measure is the Bristol Activities of Daily Living Scale; other participant outcomes include cognition, quality of life, activities of daily living and social networking; carer outcomes include quality of life, sense of competence and mental health. To enhance this quantitative evaluation we are conducting a qualitative component and a process evaluation to assess the implementation process and identify contextual factors associated with variation. DISCUSSION: The DESCANT intervention reflects current policy to enhance the capabilities of people with dementia after diagnosis and their carers. If it is clinically and cost-effective, its modest nature and cost will enhance the likelihood of it being incorporated into mainstream practice. TRIAL REGISTRATION: Current Controlled Trials, ISRCTN12591717 . Registered on 29 July 2016. Protocol number: 31288: North West - Haydock Research Ethics Committee, 20/06/2016, ref.: 16/NW/0389.
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Cognición , Demencia/terapia , Memoria , Actividades Cotidianas , Adaptación Psicológica , Demencia/diagnóstico , Demencia/psicología , Estudios de Factibilidad , Humanos , Estudios Multicéntricos como Asunto , Proyectos Piloto , Ensayos Clínicos Pragmáticos como Asunto , Calidad de Vida , Factores de Tiempo , Resultado del Tratamiento , Reino UnidoRESUMEN
BACKGROUND: The majority of residents in care homes in the United Kingdom are living with dementia or significant memory problems. Caring in this setting can be difficult and stressful for care staff who work long hours, have little opportunity for training, are poorly paid and yet subject to high expectation. This may affect their mental and physical wellbeing, cause high rates of staff turnover and absenteeism, and affect the quality of care they provide. The main objective of this survey was to explore the nature, characteristics and associations of stress in care home staff. METHODS: Staff working in a stratified random sample of care homes within Wales completed measures covering: general health and wellbeing (SF-12); stress (Work Stress Inventory); job content (Karasek Job Content); approach to, and experience of, working with people living with dementia (Approaches to Dementia Questionnaire; and Experience of Working with Dementia Patients); and Productivity and Health Status (SPS-6). Multiple linear regressions explored the effects of home and staff characteristics on carers. RESULTS: 212 staff from 72 care homes completed questionnaires. Staff from nursing homes experienced more work stress than those from residential homes (difference 0.30; 95% confidence interval (CI) from 0.10 to 0.51; P < 0.01), and were more likely to report that their health reduced their ability to work (difference -4.77; CI -7.80 to -1.73; P < 0.01). Psychological demands on nurses were higher than on other staff (difference = 1.57; CI 0.03 to 3.10; P < 0.05). A positive approach to dementia was more evident in those trained in dementia care (difference 8.54; CI 2.31 to 14.76; P < 0.01), and in staff working in local authority homes than in the private sector (difference 7.75; CI 2.56 to 12.94; P < 0.01). CONCLUSIONS: Our study highlights the importance of dementia training in care homes, with a particular need in the private sector. An effective intervention to reduce stress in health and social care staff is required, especially in nursing and larger care homes, and for nursing staff. TRIAL REGISTRATION: ISRCTN registry: ISRCTN80487202. Registered 24 July 2013.
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Identification of 'cut-points' or thresholds of climate factors would play a crucial role in alerting risks of climate change and providing guidance to policymakers. This study investigated a 'Climate Threshold' for emergency hospital admissions of chronic lower respiratory diseases by using a distributed lag non-linear model (DLNM). We analysed a unique longitudinal dataset (10 years, 2000-2009) on emergency hospital admissions, climate, and pollution factors for the Greater London. Our study extends existing work on this topic by considering non-linearity, lag effects between climate factors and disease exposure within the DLNM model considering B-spline as smoothing technique. The final model also considered natural cubic splines of time since exposure and 'day of the week' as confounding factors. The results of DLNM indicated a significant improvement in model fitting compared to a typical GLM model. The final model identified the thresholds of several climate factors including: high temperature (≥27°C), low relative humidity (≤ 40%), high Pm10 level (≥70-µg/m3), low wind speed (≤ 2 knots) and high rainfall (≥30mm). Beyond the threshold values, a significantly higher number of emergency admissions due to lower respiratory problems would be expected within the following 2-3 days after the climate shift in the Greater London. The approach will be useful to initiate 'region and disease specific' climate mitigation plans. It will help identify spatial hot spots and the most sensitive areas and population due to climate change, and will eventually lead towards a diversified health warning system tailored to specific climate zones and populations.
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Hospitalización/estadística & datos numéricos , Modelos Teóricos , Enfermedades Respiratorias/epidemiología , Contaminación del Aire/efectos adversos , Clima , Servicio de Urgencia en Hospital/estadística & datos numéricos , Humanos , Humedad , Londres/epidemiología , Estudios Longitudinales , Dinámicas no Lineales , Material Particulado/efectos adversos , Enfermedades Respiratorias/etiología , Tiempo (Meteorología) , VientoRESUMEN
A 60-year-old male reported to us with an atypical case of giant steatocystoma multiplex in the scrotum with calcification. There was no family history of similar lesions. Yellowish, creamy material was expressed from a nodule during punch biopsy. The diagnosis was based on clinical as well as histological findings. Successful surgical excision was done to cure the case without any complications.
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A polyphasic study was performed to determine the taxonomic position of strain EK05(T) isolated from a root-outgrowth of Entada koshunensis, a legume available in Okinawa, Japan. Phylogenetic analysis of the 16S rRNA gene showed that the strain belongs to the genus Bradyrhizobium. Subsequent multilocus sequence analysis with ITS, glnII, recA, gyrB, and atpD sequences revealed that the isolate represents a distinct evolutionary lineage within the genus Bradyrhizobium. DNA-DNA hybridization indicated that strain EK05(T) shares <61% DNA relatedness with the type strains of all six recognized species of Bradyrhizobium, confirming that this strain is a novel species within the genus. Phylogenetic trees based on symbiotic loci, nifH and nodC, also placed strain EK05(T) clearly in a novel branch. On the basis of its phylogenetic distinctiveness, we propose Bradyrhizobium iriomotense sp. nov. for strain EK05(T). The type strain is EK05(T) (= NBRC 102520(T) = LMG 24129(T)).
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Bradyrhizobium/metabolismo , Enzimas/aislamiento & purificación , Enzimas/metabolismo , Fabaceae/anatomía & histología , Fabaceae/enzimología , Geografía , Raíces de Plantas/enzimología , Secuencia de Bases , Enzimas/genética , Fabaceae/genética , Japón , Fenotipo , Filogenia , Raíces de Plantas/genética , ARN Ribosómico 16S/genéticaRESUMEN
Three strains, MAFF 210191(T), G24103(T) and G24116, assumed to be members of two novel species, were isolated from several rhizosphere habitats in different parts of Japan. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolates formed a distinct monophyletic group together with the two known species of the genus Labrys, suggesting that the isolates have a close affiliation with this genus. In this study, a polyphasic approach was used to characterize and compare the three isolates with the two species of the genus Labrys, Labrys monachus and Labrys methylaminiphilus. All three isolates were aerobic, Gram-negative, motile and non-sporulating and they ranged in shape from spherical to short rods. The cells multiplied by budding and utilized a wide variety of monosaccharides, disaccharides and sugar alcohols as sole carbon and energy sources, but they did not utilize C(1) compounds, salicin or d-melezitose. The strains were inhibited by dl-alpha-alanine and glycine (both at 10 mM). The major cellular fatty acids were C(19 : 0) cyclo omega8c, C(16 : 0), C(18 : 0) and C(18 : 1)omega7c. The three isolates shared <12 % and <11 % DNA-DNA relatedness with L. monachus DSM 5896(T) and L. methylaminiphilus DSM 16812(T), respectively. The G+C content of the isolates (61-62 mol%) was also significantly lower than those of the two previously characterized species. In spite of many morphological, physiological and chemotaxonomic similarities among the three isolates, strain MAFF 210191(T) could be differentiated from strains G24103(T) and G24116 on the basis of 16S rRNA gene sequence divergence, DNA-DNA relatedness (<46 %) and gelatin hydrolysis. Two novel species are therefore proposed, namely Labrys okinawensis sp. nov., with the type strain MAFF 210191(T) (=DSM 18385(T)), and Labrys miyagiensis sp. nov., with the type strain G24103(T) (=NBRC 101365(T)=NCIMB 14143(T)) and also including strain G24116 (=NBRC 101366=NCIMB 14144). Emended descriptions of the genus Labrys and Labrys monachus are also presented.