RESUMEN
The influence of exogenic human recombinant tumor necrosis factor (TNF-alpha) on antibody production in mice immunized with the preparation of F.tularensis outer membranes (OM) was studied. TNF-alpha was injected into mice in doses of 0.001-10 I.U. before and simultaneously with the injection of the preparation of F.tularensis OM. The levels of tularemia antibodies, determined by ELISA techniques, and the number of antibody-producing cells (APC) were studied. The study revealed that recombinant TNF-alpha in the range of doses used in this investigation stimulated the formation of humoral immunity. The injection of TNF-alpha in a dose of 0.001 I.U. was found to produce the most pronounced effect on the level of tularemia antibodies and the number of antibody-producing cells. The use TNF-alpha as immunomodulator made it possible to decrease the dose of the preparation of F.tularensis OM introduced as immunogenic agent without essential changes in the number of splenic APC and in the level of tularemia antibodies.
Asunto(s)
Anticuerpos Antibacterianos/efectos de los fármacos , Proteínas de la Membrana Bacteriana Externa/inmunología , Francisella tularensis/inmunología , Inmunización/métodos , Factor de Necrosis Tumoral alfa/farmacología , Animales , Anticuerpos Antibacterianos/biosíntesis , Anticuerpos Antibacterianos/sangre , Células Productoras de Anticuerpos/efectos de los fármacos , Células Productoras de Anticuerpos/inmunología , Relación Dosis-Respuesta a Droga , Relación Dosis-Respuesta Inmunológica , Femenino , Humanos , Masculino , Ratones , Ratones Endogámicos CBA , Proteínas Recombinantes/farmacología , Estimulación Química , Factores de TiempoAsunto(s)
Peste/etiología , Factor de Necrosis Tumoral alfa/farmacología , Animales , Citotoxicidad Celular Dependiente de Anticuerpos/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Humanos , Sueros Inmunes/farmacología , Ratones , Ratones Endogámicos CBA , Peste/inmunología , Ratas , Proteínas Recombinantes/farmacología , Factores de Tiempo , Factor de Necrosis Tumoral alfa/inmunologíaRESUMEN
The activity of NK and K cells in mice immunized against plague has been studied. This activity has been shown to depend on the dose of the vaccine and the time elapsed after immunization. The booster immunization of mice leads to an increase in the specific sensitization of lymphocytes, the antibody level and to an increase in the activity of killer cells. Changes in the cytotoxicity of NK cells on days 21-28 after primary and booster immunization are considered to occur not due to the action of Yersinia pestis themselves, but as the result of the immunological transformation induced by these microorganisms in the animals.
Asunto(s)
Células Asesinas Naturales/inmunología , Vacuna contra la Peste/inmunología , Peste/prevención & control , Animales , Anticuerpos Antibacterianos/sangre , Relación Dosis-Respuesta Inmunológica , Tolerancia Inmunológica , Inmunización Secundaria , Inyecciones Subcutáneas , Activación de Linfocitos , Ratones , Ratones Endogámicos CBA , Vacuna contra la Peste/administración & dosificación , Factores de Tiempo , Yersinia pestis/inmunologíaRESUMEN
In this work the conditions of labeling protein A with europium ions were studied and the conjugates obtained in this study were compared with traditional peroxidase conjugates currently used in immunochemistry. The conjugates of protein A with Eu3+ chelate were obtained with the use of cyclic dianhydride of diethylenetriaminepentaacetic acid (DADETPA). Conjugation methods with the use of DADETPA was shown to permit obtaining high-quality conjugates with europium chelates. Europium-labeled protein A ensured the sensitivity of the determination of adsorbed IgG at a level of 2 ng/ml and the dynamic analytical range within 3-1,000 ng/ml, which essentially exceeded similar characteristics of peroxidase conjugates with protein A. Europium-labeled protein A was used for the detection of antibodies to Francisella tularensis in the sera of humans immunized against tularemia. The sensitivity of this assay exceeded that of the enzyme immunoassay 10- to 40-fold. A conclusion was made on the possibility of using europium labelled protein A for the determination of specific antibodies to F.tularensis. This preparation may be useful in the determination of specific antibodies in low-immune sera.
Asunto(s)
Anticuerpos Antibacterianos/sangre , Especificidad de Anticuerpos , Francisella tularensis/inmunología , Proteína Estafilocócica A , Adolescente , Adulto , Quelantes , Europio , Estudios de Evaluación como Asunto , Humanos , Técnicas para Inmunoenzimas , Inmunoglobulina M/sangre , Indicadores y Reactivos , Masculino , Ácido Pentético , Proteínas Recombinantes , Sensibilidad y EspecificidadRESUMEN
Changes in the activity of NK and K cells in persons immunized against plague have been studied. A decrease in the activity of natural killer cells has been shown to occur. Booster immunization leads to a greater increase in the specific sensitization of lymphocytes, as well as in the antibody level. The observed increase of the activity of killer cells is regarded not as the result of the direct action of live Yersinia pestis cells, but as the result of the immunological changes in the body which they produce.
Asunto(s)
Inmunización Secundaria , Células Asesinas Naturales/inmunología , Vacuna contra la Peste/inmunología , Peste/prevención & control , Adulto , Anticuerpos Antibacterianos/sangre , Pruebas Inmunológicas de Citotoxicidad , Humanos , Activación de Linfocitos , Masculino , Peste/inmunología , Factores de Tiempo , Yersinia pestis/inmunologíaRESUMEN
The opsonizing properties of sera obtained from hamadryas baboons immunized with the preparation of F. tularensis outer membranes (OM) were studied with the use of luminol-dependent chemiluminescence (CL) of whole blood. The immunization of monkeys with the OM preparation was shown to lead to the formation of functionally active antibodies possessing opsonizing properties with respect to virulent F. tularensis. Immune sera obtained from the animals immunized with live vaccine and from those immunized with OM preparation had no essential differences in their opsonizing properties. The level of IgG antibodies in immune sera correlated with the CL parameters of whole blood in the presence of F. tularensis opsonized with these sera. Increased CL of phagocytes observed after addition of bacteria and immune sera under test to whole blood taken from a nonimmune donor made it possible to evaluate the functional activity of antibodies, thus permitting its use as a test for the evaluation of the effectiveness of new vaccine preparations.
Asunto(s)
Proteínas de la Membrana Bacteriana Externa/inmunología , Francisella tularensis/inmunología , Sueros Inmunes/inmunología , Inmunización/métodos , Proteínas Opsoninas/inmunología , Papio/inmunología , Animales , Anticuerpos Antibacterianos/sangre , Proteínas de la Membrana Bacteriana Externa/aislamiento & purificación , Vacunas Bacterianas/inmunología , Relación Dosis-Respuesta Inmunológica , Femenino , Francisella tularensis/patogenicidad , Mediciones Luminiscentes , Luminol , Masculino , Factores de TiempoRESUMEN
Antibody formation in animals immunized with one of the components of F. tularensis surface structures was studied. The time course of antibody formation in 20 hamadryas baboons was studied in the passive hemagglutination (PHA) test, microagglutination (MA) test, and indirect enzyme immunoassay, used for the determination of IgG, IgA and IgM antibodies. The character of antibody response in the animals immunized with components of F. tularensis surface structures (S-complex) and with live tularemia vaccine was compared. The study revealed that immunization with the S-complex induced the formation of antibodies detected by all three methods. Antibody formation to the S-complex was found to be dose-dependent. With the increase of the injected dose of the S-complex, antibody titers determined in the PHA test decreased and those determined in the MA test increased, which was seemingly due to the induction of antibodies differing in their isotypes. After immunization with the S-complex the levels of IgG antibodies were lower and the levels of IgM antibodies by day 28 after immunization higher than after the injection of live tularemia vaccine.
Asunto(s)
Anticuerpos Antibacterianos/biosíntesis , Antígenos Bacterianos/inmunología , Vacunas Bacterianas/inmunología , Francisella tularensis/inmunología , Inmunización/métodos , Animales , Anticuerpos Antibacterianos/sangre , Antígenos de Superficie/inmunología , Relación Dosis-Respuesta Inmunológica , Femenino , Inmunoglobulina A/sangre , Inmunoglobulina G/sangre , Isotipos de Inmunoglobulinas/sangre , Inmunoglobulina M/sangre , Masculino , Papio , Factores de TiempoRESUMEN
The possibility of using the micropoint enzyme immunoassay (EIA) on a nitrocellulose membrane with the visual evaluation of results for the detection of tularemia IgG antibodies in hamadryas baboons at the postvaccinal period has been studied. The sensitivity of this assay has been compared with that of the passive hemagglutination (PHA) test, the microagglutination (MA) test and EIA with the spectrophotometric evaluation of results in plates. As shown in this study, EIA in the above-mentioned modification can be successfully used for the detection of tularemia antibodies in the blood serum. The sensitivity of micropoint EIA has proved to be not inferior to that of EIA in plates, while exceeding the sensitivity of the PHA test 10- to 20-fold and the sensitivity of the MA test 10- to 1,000-fold. This method is simple, reliable, highly sensitive, economic and requires no special equipment, which makes it highly promising for the diagnosis of tularemia and the evaluation of humoral immunity at the postvaccinal period.
Asunto(s)
Anticuerpos Antibacterianos/sangre , Francisella tularensis/inmunología , Immunoblotting/métodos , Técnicas para Inmunoenzimas , Animales , Vacunas Bacterianas/inmunología , Colodión , Estudios de Evaluación como Asunto , Femenino , Inmunización/métodos , Immunoblotting/instrumentación , Técnicas para Inmunoenzimas/instrumentación , Masculino , Membranas Artificiales , Papio , Factores de TiempoRESUMEN
The efficacies of two methods for measuring human blood serum IgG, heterogenic enzyme immunoassay (EIA) and radial immunodiffusion in gel (RIG), are compared. The accuracy of both the methods was verified by the data of IgG spectrophotometry; IgG were isolated by ion exchange chromatography from human blood serum. The results of all the three methods were in high correlation: correlation coefficient of spectrophotometry and EIA data was 0.992, p less than 0.01; that of spectrophotometry and RIG data 0.975, p less than 0.01; that of RIG and EIA 0.888, p less than 0.01. Since EIA has some advantages over RIG (it is more rapid, sensitive, accurate, and the investigation may be automated), it is recommended for measuring human blood serum IgG in mass screenings.