RESUMEN
The establishment of cellular identity is driven by transcriptional and epigenetic regulators of the chromatin proteome - the chromatome. Comprehensive analyses of the chromatome composition and dynamics can therefore greatly improve our understanding of gene regulatory mechanisms. Here, we developed an accurate mass spectrometry (MS)-based proteomic method called Chromatin Aggregation Capture (ChAC) followed by Data-Independent Acquisition (DIA) and analyzed chromatome reorganizations during major phases of pluripotency. This enabled us to generate a comprehensive atlas of proteomes, chromatomes, and chromatin affinities for the ground, formative and primed pluripotency states, and to pinpoint the specific binding and rearrangement of regulatory components. These comprehensive datasets combined with extensive analyses identified phase-specific factors like QSER1 and JADE1/2/3 and provide a detailed foundation for an in-depth understanding of mechanisms that govern the phased progression of pluripotency. The technical advances reported here can be readily applied to other models in development and disease.
Asunto(s)
Cromatina , Células Madre Embrionarias , Células Madre Pluripotentes , Proteómica , Cromatina/genética , Espectrometría de Masas/métodos , Proteoma/genética , Proteoma/metabolismo , Proteómica/métodos , Humanos , Animales , Ratones , Células Madre Embrionarias/citología , Células Madre Embrionarias/metabolismo , Células Madre Pluripotentes/citología , Células Madre Pluripotentes/metabolismoRESUMEN
Copper(II) complexes with 2,5-bis(methylthio)-1,3,4-thiadiazole (tda) formulated as [Cu(tda)n X2 ] (n=2, X=Cl- , Br- , C2 N3 - ; n= 1, X=C2 N3 - ) have been isolated and fully characterized. The crystal structures of all compounds have been determined using single-crystal X-ray diffraction (SCXRD). A study of the magnetic susceptibility in the range 1.77-300â K has shown that magnetic properties of the [Cu(tda)2 Cl2 ] and [Cu(tda)2 Br2 ] complexes match those of 1D chains of antiferromagnetically-coupled Cu2+ ions. The intrachain interaction J in [Cu(tda)2 Cl2 ] turns out to be â¼1.2 times weaker than in its bromide analogue. In its turn, [Cu(tda)2 (C2 N3 )2 ] exhibits J being an order of magnitude smaller and of the opposite ferromagnetic sign. Halogen bonding (HB) between adjacent complexes is much stronger than the H-bonds or π-π interactions between tda ligands according to the DFT calculations.
