Characterization and synergy studies of Caudoviricete Escherichia phage FS2B infecting multi-drug resistant uropathogenic Escherichia coli isolates.

Escherichia coli is one of the most common causes of urinary tract infections. However, a recent upsurge in antibiotic resistance among uropathogenic E. coli (UPEC) strains has provided an impetus to explore alternative antibacterial compounds to encounter this major issue. In this study, a lytic phage against multi-drug-resistant (MDR) UPEC strains was isolated and characterized. The isolated Escherichia phage FS2B of class Caudoviricetes exhibited high lytic activity, high burst size, and a small adsorption and latent time. The phage also exhibited a broad host range and inactivated 69.8% of the collected clinical, and 64.8% of the identified MDR UPEC strains. Further, whole genome sequencing revealed that the phage was 77,407 bp long, having a dsDNA with 124 coding regions. Annotation studies confirmed that the phage carried all the genes associated with lytic life cycle and all lysogeny related genes were absent in the genome. Further, synergism studies of the phage FS2B with antibiotics demonstrated a positive synergistic association among them. The present study therefore concluded that the phage FS2B possesses an immense potential to serve as a novel candidate for treatment of MDR UPEC strains.

Bryophyllum pinnatum mediated synthesis of zinc oxide nanoparticles: characterization and application as biocontrol agents for multi-drug-resistant uropathogens.

The emerging era of antimicrobial resistance has become a challenge for the potentiality of current antibiotic therapy, making the treatment of several diseases, including urinary tract infections (UTIs) very chaotic. To combat the present circumstances, there is an urge among the scientific community to find efficient substitutes for antibiotic therapy, which may potentially delimit the antimicrobial resistance among the various uropathogens. In this direction, the upcoming field of nanotechnology holds a high potential. Therefore, the present study aimed at the evaluation of the antimicrobial potential of green synthesized zinc oxide nanoparticles. The nanoparticles were synthesized using Bryophyllum pinnatum plant leaf extract and were characterized with the help of several analytical techniques. A sharp peak obtained at 369 nm by UV-Visible spectroscopy affirmed the synthesis of Bryophyllum- ZnO nanoparticles, and the FTIR spectroscopy confirmed the conjugation of different phytochemicals. XRD analysis revealed the crystallinity and hexagonal conformation, and through SEM and HR-TEM, the particle size of the synthesized Bryophyllum- ZnO nanoparticles was found to be between 14-35 nm. The synthesized green nanoparticles, when tested against a few highly MDR uropathogenic bacteria (E. coli, E. furgusonii, K. pneumoniae, S. flexneri, and P. aeruginosa), were observed to exhibit high antimicrobial response (zones of inhibition ranging between 22 mm to 28 mm), thus confirming that these were bestowed with potent antimicrobial ability. Hence, from the present work, it could be concluded that Bryophyllum- ZnO nanoparticles can be used as potential nanoantibiotic sources to deal with UTIs.