Nutrient cycling enhancement in intensive-extensive aquaculture through C/N ratio manipulation and periphyton support.

The external carbon source and the installation of periphyton structures were applied in combined intensive-extensive aquaculture to test their efficiency of nutrient utilization to support clean and efficient fish production. Two aquaculture systems were tested, with one additionally treated with methanol as a source of carbohydrates for microbial activity stimulation and an additional area for periphyton installed. Each system was composed of fish tanks with intensively reared sturgeon and one extensive pond stocked with common carp in polyculture. The water from intensive fish production was discharged into the fish pond, to serve as a nutrient source for primary production in the pond. Obtained data revealed that applied manipulations enhanced microorganism development and pond productivity. The results of the research show that applied moderate, nature-based upgrade in aquaculture system may allow for more efficient and cost-effective treatment of wastewater from intensive aquaculture.

An In Vitro Comparative Study of the Effects of Tetrabromobisphenol A and Tetrabromobisphenol S on Human Erythrocyte Membranes-Changes in ATP Level, Perturbations in Membrane Fluidity, Alterations in Conformational State and Damage to Proteins.

Brominated flame retardants (BFRs) are substances used to reduce the flammability of plastics. Among this group, tetrabormobisphenol A (TBBPA) is currently produced and used on the greatest scale, but due to the emerging reports on its potential toxicity, tetrabromobisphenol S (TBBPS)-a compound with a very similar structure-is used as an alternative. Due to the fact that the compounds in question are found in the environment and in biological samples from living organisms, including humans, and due to the insufficient toxicological knowledge about them, it is necessary to assess their impacts on living organisms and verify the validity of TBBPA replacement by TBBPS. The RBC membrane was chosen as the research model. This is a widely accepted research model for assessing the toxicity of xenobiotics, and it is the first barrier to compounds entering circulation. It was found that TBBPA and TBBPS caused increases in the fluidity of the erythrocyte membrane in their hydrophilic layer, and conformational changes to membrane proteins. They also caused thiol group elevation, an increase in lipid peroxidation (TBBPS only) and decreases in the level of ATP in cells. They also caused changes in the size and shape of RBCs. TBBPA caused changes in the erythrocyte membrane at lower concentrations compared to TBBPS at an occupational exposure level.

The enhancement of valley water retentiveness in climate change conditions.

River system retentiveness must be enhanced to increase multidimensional environmental sustainability and thus ameliorate the effects of climate change and the occurrence of extreme hydrological events. The aim of the article is to demonstrate how ecohydrological Nature-Based Solutions can be combined with conventional infrastructure to improve WBSRCE benefits (Water, Biodiversity, ecosystem Services, Resilience, Culture, Education) by taking a holistic approach to multifunctional reservoir design. The paper proposes a new form of lateral reservoir, which is built without disturbing the meandering river and is supplied with good quality water through a monitoring and regulatory system; its design is based on thorough analysis of hydrological pulses and suspended matter and nutrients fluxes. The regulatory system also includes an innovative Sequential Sedimentation and Biofiltration System. Lateral reservoirs increase water retentiveness in the river valley by lifting ground water level and restoring surrounding wetlands, thus enhancing biodiversity, ecosystem services for society and the resilience of the river system to climate change. An integrative understanding of the interplay between hydrology and biocenosis can be used to enhance river system sustainability potential (WBSR) and harmonise societal needs with biosphere sustainability through culture and education (CE).

Evaluation of apoptotic potential of glyphosate metabolites and impurities in human peripheral blood mononuclear cells (in vitro study).

Glyphosate is used for cereal, vegetable and fruit crops for reducing or inhibiting the growth of weeds as well as a desiccant for various grain crops. That is why, glyphosate has been shown to be accumulated in humans and animals through ingestion of food of both plant and animal origin. The study aimed to assessed the effect of glyphosate, its metabolites: aminomethylphosphonic acid (AMPA), methylphosphonic acid and its impurities: PMIDA, N-methylglyphosate, hydroxymethylphosphonic acid and bis(phosphonomethyl)amine on apoptosis induction in human peripheral blood mononuclear cells (PBMCs). PBMCs were exposed to the compounds studied at the concentrations ranging from 0.01 to 5 mM for 4 h. We have observed an increase in reactive oxygen species (including hydroxyl radical) and cytosolic calcium ions levels as well as reduction of transmembrane mitochondrial potential (ΔΨm) in PBMCs exposed to the compounds examined. All substances studied changed PBMCs membrane permeability, activated caspase-8, -9, -3 and caused chromatin condensation, which showed that they were capable of inducing apoptosis both via extrinsic and particularly intrinsic pathway. Generally the study demonstrated that there were no differences between apoptotic changes induced by glyphosate, its metabolites or impurities, and observed changes were provoked by high concentrations of investigated compounds.

The Impact of Glyphosate, Its Metabolites and Impurities on Viability, ATP Level and Morphological changes in Human Peripheral Blood Mononuclear Cells.

The toxicity of herbicides to animals and human is an issue of worldwide concern. The present study has been undertaken to assess toxic effect of widely used pesticide-glyphosate, its metabolites: aminomethylphosphonic acid (AMPA) and methylphosphonic acid and its impurities: N-(phosphonomethyl)iminodiacetic acid (PMIDA), N-methylglyphosate, hydroxymethylphosphonic acid and bis-(phosphonomethyl)amine on human peripheral blood mononuclear cells (PBMCs). We have evaluated the effect of those compounds on viability, ATP level, size (FSC-A parameter) and granulation (SSC-A parameter) of the cells studied. Human peripheral blood mononuclear cells were exposed to different concentrations of glyphosate, its metabolites and impurities (0.01-10 mM) for 4 and 24 h. It was found that investigated compounds caused statistically significant decrease in viability and ATP level of PBMCs. The strongest changes in cell viability and ATP level were observed after 24 h incubation of PBMCs with bis-(phosphonomethyl)amine, and particularly PMIDA. Moreover, all studied compounds changed cell granularity, while PMIDA and bis-(phosphonomethyl)amine altered PBMCs size. It may be concluded that bis-(phosphonomethyl)amine, and PMIDA caused a slightly stronger damage to PBMCs than did glyphosate. Changes in the parameters studied in PBMCs were observed only at high concentrations of the compounds examined, which clearly shows that they may occur in this cell type only as a result of acute poisoning of human organism with these substances.