RESUMEN
In the present study, the complete mitochondrial genome of Trachypithecus francoisi was determined using PCR reactions. The mitochondrial genome is 16,544 bp in length, consisting of 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a control region. The structural organization and gene order of T. francoisi were equivalent to that of most other vertebrates. The T. francoisi mtDNA could provide useful data for further studies on phylogenetics and conservation genetics of this species.
Asunto(s)
Colobinae/genética , ADN Mitocondrial/genética , Genoma Mitocondrial , Animales , Secuencia de Bases , Filogenia , Reacción en Cadena de la Polimerasa , Análisis de Secuencia de ADN/veterinariaRESUMEN
In this study, the complete mitochondrial genome of Trachypithecus cristatus was determined using PCR method. The mitochondrial genome is 16,557 bp in length, it containing 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes and a control region. The structural organization and gene order of T. cristatus mtDNA were similar to that of most other vertebrates. The results could provide useful information for further studies on phylogenetics of T. cristatus.
Asunto(s)
Colobinae/genética , Genes Mitocondriales/fisiología , Genoma Mitocondrial/fisiología , Animales , Secuencia de Bases , ADN Mitocondrial/genética , Proteínas Mitocondriales/genética , Datos de Secuencia Molecular , ARN/genética , ARN Mitocondrial , ARN Ribosómico/genética , ARN de Transferencia/genéticaRESUMEN
To investigate the functions of U6 and 7SK of Bama mini-pig and produce Bama mini-pig with silenced GGTA1 gene, the siRNA promoters U6 and 7SK were cloned, ligated into pMD18-shEGFP, and co-transfected with PEGFP- N1 into PK-15 kidney cells of pigs to be used in RNAi experiments. The functions of the two promoters in pig cells were verified using pMD18-hU6-shEGFP as the positive control, pMD18-shEGFP vector without promoter as the negative control, PEGFP-N1 as the first blank control, ddH2O in replacement of the plasmid as the second blank control. The results showed that the lengths of U6 and 7SK in Bama mini-pig were 553 bp and 437 bp, respectively. Vectors pMD18-pU6- shEGFP and pMD18-p7SK-shEGFP were constructed and transfected into PK-15 cells from pigs. Promoters pU6 and p7SK proved to express high levels of siRNA activity and can be used in the experiment of silencing α-1,3galactosyltransferase gene.
Asunto(s)
Regiones Promotoras Genéticas/fisiología , ARN Polimerasa III/genética , ARN Interferente Pequeño/genética , Porcinos Enanos/genética , Animales , Secuencia de Bases , Clonación Molecular , Datos de Secuencia Molecular , Análisis de Secuencia de ADN , PorcinosRESUMEN
The mitochondrial COI gene was PCR amplified and sequenced from 17 samples obtained from three populations of Macrobrachium rosenbergii (F1 of the Burma wildtype population, Jiangsu cultured population and F2 of the Guangxi breeding population). A 498-bp long partial gene segment was acquired and used to study the genetic diversity among the three populations. Results indicated that the COI gene locus was relatively more polymorphic in the F1 of Burma wildtype population, while the polymorphism in Jiangsu cultured population and Guangxi breeding population were very poor. A total of 10 polymorphic sites and 5 haplotypes were found in the sequences of the 17 samples. The average nucleotide divergence in the three populations was 0.88%0.07% and 0 respectively. The UPGMA phylogenetic tree suggested that F2 of the Guangxi breeding population and Jiangsu cultured population were closest genetically, and their haplotypes could be gathered together to a genetic branch while F1 of the Burma wildtype population diverged and could form another relatively independent branch. For these distinct nucleotide differences, COI gene could be suitable as a genetic marker for distinguishing these two branches of the Macrobrachium rosenbergii population.
