Synergistic bactericidal effect and mechanism of ultrasound combined with Lauroyl Arginate Ethyl against Salmonella Typhimurium and its application in the preservation of onions.

In the present study, the synergistic bactericidal effect and mechanism of ultrasound (US) combined with Lauroyl Arginate Ethyl (LAE) against Salmonella Typhimurium were investigated. On this basis, the effect of US+LAE treatment on the washing of S. Typhimurium on the surface of onions and on the physical and chemical properties of onion during fresh-cutting and storage were studied. The results showed that treatment with US+LAE could significantly (P < 0.05) reduce the number of S. Typhimurium compared to US and LAE treatments alone, especially the treatment of US+LAE (230 W/cm2, 8 min, 71 µM) reduced S. Typhimurium by 8.82 log CFU/mL. Confocal laser scanning microscopy (CLSM), flow cytometry (FCM), protein and nucleic acid release and N-phenyl-l-naphthylamine (NPN) assays demonstrated that US+LAE disrupted the integrity and permeability of S. Typhimurium cell membranes. Reactive oxygen species (ROS) and malondialdehyde (MDA) assays indicated that US+LAE exacerbated oxidative stress and lipid peroxidation in cell membranes. Field emission scanning electron microscopy (FESEM) demonstrated that US+LAE treatment caused loss of cellular contents and led to cell crumpling and even lost the original cell morphology. US+LAE treatment caused a significant (P < 0.05) decrease in the number of S. Typhimurium on onions, but there was no significant (P > 0.05) effect on the color, hardness, weight and ascorbic acid content of onions. This study elucidated the synergistic antibacterial mechanism of US+LAE and verified the feasibility of bactericidal effect on the surface of onions, providing a theoretical basis for improving the safety of fresh produce in the food industry and to propose a new way to achieve the desired results.

Antibacterial effect of ultrasound and ß-citronellol against Listeria monocytogenes and its application in carrot preservation.

This study investigated the antibacterial effects of ultrasound (US), ß-citronellol (CT), and a combination of the two treatments on Listeria monocytogenes. Results showed that US or CT alone did not show apparent antibacterial effect (0.02-0.76 log CFU/mL reduction). The combined treatment showed obviously inactivate effect of L. monocytogenes, the populations of L. monocytogenes decreased by 8.93 log CFU/mL after US (253 W/cm2, 20 kHz) + 0.8 mg/mL CT treatment. US + CT treatment also had a significant (P < 0.05) antibacterial effect on isolates of L. monocytogenes from three different serotypes. In this study, the damage of US + CT on cell morphology had been observed using field emission scanning electron microscopy, while the damage to cell membranes by US + CT was observed by confocal laser scanning microscopy and flow cytometry. Meanwhile, the uptake of N-phenyl-l-naphthylamine and the absorbance at 260 and 280 nm also indicated that the combined treatment disrupted the permeability and integrity of L. monocytogenes membranes. Reactive oxygen species and malondialdehyde assays showed that US + CT exacerbated cellular oxidative stress and lipid peroxidation. In addition, the US + CT treatment reduced L. monocytogenes by 3.14-4.24 log CFU/g on the surface of carrots. Total phenolic and carotenoid contents in carrots were elevated after US + CT treatment. During storage, compared to control, US + CT did not significantly (P > 0.05) change the surface color of carrots but significantly (P < 0.05) decreased both hardness and weight, and has an impact on the sensory. This study showed that US + CT is a promising cleaning method that will provide new ideas for the preservation of fresh agricultural produce.