RESUMEN
Multiple linear regression (MLR) models were developed for predicting chronic zinc toxicity to a freshwater microalga, Chlorella sp., using three toxicity-modifying factors (TMFs): pH, hardness, and dissolved organic carbon (DOC). The interactive effects between pH and hardness and between pH and DOC were also included. Models were developed at three different effect concentration (EC) levels: EC10, EC20, and EC50. Models were independently validated using six different zinc-spiked Australian natural waters with a range of water chemistries. Stepwise regression found hardness to be an influential TMF in model scenarios and was retained in all final models, while pH, DOC, and interactive terms had variable influence and were only retained in some models. Autovalidation and residual analysis of all models indicated that models generally predicted toxicity and that there was little bias based on individual TMFs. The MLR models, at all effect levels, performed poorly when predicting toxicity in the zinc-spiked natural waters during independent validation, with models consistently overpredicting toxicity. This overprediction may be from another unaccounted for TMF that may be present across all natural waters. Alternatively, this consistent overprediction questions the underlying assumption that models developed from synthetic laboratory test waters can be directly applied to natural water samples. Further research into the suitability of applying synthetic laboratory water-based models to a greater range of natural waters is needed. Environ Toxicol Chem 2023;42:2630-2641. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
Asunto(s)
Chlorella , Microalgas , Contaminantes Químicos del Agua , Modelos Lineales , Concentración de Iones de Hidrógeno , Australia , Agua Dulce , Agua , Contaminantes Químicos del Agua/toxicidad , Compuestos Orgánicos , Zinc/toxicidadRESUMEN
Bioavailability models, for example, multiple linear regressions (MLRs) of water quality parameters, are increasingly being used to develop bioavailability-based water quality criteria for metals. However, models developed for the Northern Hemisphere cannot be adopted for Australia and New Zealand without first validating them against local species and local water chemistry characteristics. We investigated the applicability of zinc chronic bioavailability models to predict toxicity in a range of uncontaminated natural waters in Australia and New Zealand. Water chemistry data were compiled to guide a selection of waters with different zinc toxicity-modifying factors. Predicted toxicities using several bioavailability models were compared with observed chronic toxicities for the green alga Raphidocelis subcapitata and the native cladocerans Ceriodaphnia cf. dubia and Daphnia thomsoni. The most sensitive species to zinc in five New Zealand freshwaters was R. subcapitata (72-h growth rate), with toxicity ameliorated by high dissolved organic carbon (DOC) or low pH, and hardness having a minimal influence. Zinc toxicity to D. thomsoni (reproduction) was ameliorated by both high DOC and hardness in these same waters. No single trophic level-specific effect concentration, 10% (EC10) MLR was the best predictor of chronic toxicity to the cladocerans, and MLRs based on EC10 values both over- and under-predicted zinc toxicity. The EC50 MLRs better predicted toxicities to both the Australian and New Zealand cladocerans to within a factor of 2 of the observed toxicities in most waters. These findings suggest that existing MLRs may be useful for normalizing local ecotoxicity data to derive water quality criteria for Australia and New Zealand. The final choice of models will depend on their predictive ability, level of protection, and ease of use. Environ Toxicol Chem 2023;42:2614-2629. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
Asunto(s)
Cladóceros , Contaminantes Químicos del Agua , Animales , Modelos Lineales , Nueva Zelanda , Concentración de Iones de Hidrógeno , Australia , Compuestos Orgánicos , Zinc/toxicidad , Agua Dulce , Contaminantes Químicos del Agua/toxicidadRESUMEN
Anthropogenic activities in Antarctica have led to contamination of terrestrial sites, and soils in ice-free areas have elevated concentrations of metals, particularly around current and historic research stations. Effective management of Antarctic contaminated sites depends on the assessment of risks to a representative range of native terrestrial species. Bdelloid rotifers are an abundant and biodiverse component of Antarctic limnoterrestrial communities and play a key role in nutrient cycling in Antarctic ecosystems. The present study investigates the toxicity of five metals (cadmium, copper, nickel, lead, and zinc) to the endemic bdelloid rotifer Adineta editae, both singly and in metal mixtures. Based on the concentrations tested, zinc was the most toxic metal to survival with a 7-day median lethal concentration (LC50) of 344 µg Zn/L, followed by cadmium with a 7-day LC50 of 1542 µg Cd/L. Rotifers showed high sensitivity using cryptobiosis (chemobiosis) as a sublethal behavioral endpoint. Chemobiosis was triggered in A. editae at low metal concentrations (e.g., 6 µg/L Pb) and is likely a protective mechanism and survival strategy to minimize exposure to stressful conditions. Lead and copper were most toxic to rotifer behavior, with 4-day median effect concentrations (EC50s) of 18 and 27 µg/L, respectively, followed by zinc and cadmium (4-day EC50 values of 52 and 245 µg/L, respectively). The response of rotifers to the metal mixtures was antagonistic, with less toxicity observed than was predicted by the model developed from the single-metal exposure data. The present study provides evidence that this bdelloid rotifer represents a relatively sensitive microinvertebrate species to metals and is recommended for use in contaminant risk assessments in Antarctica. Environ Toxicol Chem 2023;42:1409-1419. © 2023 SETAC.
Asunto(s)
Cobre , Contaminantes Químicos del Agua , Cobre/toxicidad , Cadmio/toxicidad , Regiones Antárticas , Ecosistema , Contaminantes Químicos del Agua/toxicidad , Metales/toxicidad , Zinc/toxicidadRESUMEN
Microalgae are key components of aquatic food chains and are known to be sensitive to a range of contaminants. Much of the available data on metal toxicity to microalgae have been derived from temperate single-species tests with temperate data used to supplement tropical toxicity data sets to derive guideline values. In the present study, we used single-species and multispecies tests to investigate the toxicity of nickel and copper to tropical freshwater and marine microalgae, including the free-swimming stage of Symbiodinium sp., a worldwide coral endosymbiont. Based on the 10% effect concentration (EC10) for growth rate, copper was two to four times more toxic than nickel to all species tested. The temperate strain of Ceratoneis closterium was eight to 10 times more sensitive to nickel than the two tropical strains. Freshwater Monoraphidium arcuatum was less sensitive to copper and nickel in the multispecies tests compared with the single-species tests (EC10 values increasing from 0.45 to 1.4 µg Cu/L and from 62 to 330 µg Ni/L). The Symbiodinium sp. was sensitive to copper (EC10 of 3.1 µg Cu/L) and less sensitive to nickel (EC50 >1600 µg Ni/L). This is an important contribution of data on the chronic toxicity of nickel to Symbiodinium sp. A key result from the present study was that three microalgal species had EC10 values below the current copper water quality guideline value for 95% species protection in slightly to moderately disturbed systems in Australia and New Zealand, indicating that they may not be adequately protected by the current copper guideline value. By contrast, toxicity of nickel to microalgae is unlikely to occur at exposure concentrations typically found in fresh and marine waters. Environ Toxicol Chem 2023;42:901-913. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
Asunto(s)
Microalgas , Contaminantes Químicos del Agua , Níquel/toxicidad , Níquel/análisis , Cobre/toxicidad , Agua Dulce , Calidad del Agua , Contaminantes Químicos del Agua/toxicidad , Contaminantes Químicos del Agua/análisisRESUMEN
Zinc is a contaminant of concern in aquatic environments and is a known toxicant to many aquatic organisms. Dissolved organic matter (DOM) is a toxicity modifying factor for zinc and is an important water chemistry parameter. This study investigated the influence of DOM concentration, source, and water pH on the chronic toxicity of zinc to a freshwater microalga, Chlorella sp. The influence of DOM on zinc toxicity was dependent on both concentration and source. In the absence of DOM, the 72-h EC50 was 112 µg Zn.L-1. In the presence of a DOM high in fulvic-like components, zinc toxicity was either slightly decreased (<4-fold increase in EC10s across 15 mg C.L-1 range) or unchanged (minimal difference in EC50s). In the presence of a DOM high in humic-like (aromatic and high molecular weight) components, zinc toxicity was slightly decreased at the EC10 level and strongly increased at the EC50 level. The influence of pH on zinc toxicity was dependent on the source of DOM present in the water. In the presence of DOM high in humic-like components pH did not influence toxicity. In the presence of DOM high in fulvic-like components, pH had a significant effect on EC50 values. Labile zinc (measured by diffusive gradients in thin-films) followed linear relationships with dissolved zinc but could not explain the changes in observed toxicity, with similar DGT-labile zinc relationships shown for the two DOMs despite each DOM influencing toxicity differently. This indicates changes in toxicity may be unrelated to changes in zinc lability. The results suggest that increased toxicity of zinc in the presence of DOM may be due to direct uptake of Zn-DOM complexes. This study highlights the importance of considering DOM source and characteristics when incorporating DOM into water quality guidelines through bioavailability models.
Asunto(s)
Chlorella , Microalgas , Zinc/toxicidad , Agua Dulce/química , Compuestos Orgánicos , Materia Orgánica Disuelta , Concentración de Iones de HidrógenoRESUMEN
Zinc is an essential element for aquatic organisms, however, activities such as mining and refining, as well as zinc's ubiquitous role in modern society can contribute to elevated environmental concentrations of zinc. Water hardness is widely accepted as an important toxicity modifying factor for metals in aquatic systems, though other factors such as pH are also important. This study investigated the influence of increasing water hardness, at three different pH values (6.7, 7.6 and 8.3), on the chronic toxicity of zinc to the growth rate of a microalgae, Chlorella sp. Zinc toxicity decreased with increasing hardness from 5 to 93 mg CaCO3 L-1 at all three pH values tested. The 72 h growth rate inhibition EC50 values ranged from 6.2 µg Zn L-1 (at 5 mg CaCO3 L-1, pH 8.3) to 184 µg Zn L-1 (at 92 mg CaCO3 L-1, pH 6.7). Increases in hardness from 93 to 402 mg CaCO3 L-1 generally resulted in no significant (p > 0.05) reduction in zinc toxicity. DGT-labile zinc measurements did not correspond with the observed changes in zinc toxicity as hardness was varied within a pH treatment. This suggests that cationic competition from increased hardness is decreasing zinc toxicity, rather than changes in metal lability. This study highlighted that current hardness algorithms used in water quality guidelines may not be sufficiently protective of sensitive species, such as Chlorella sp., in high hardness waters.
Asunto(s)
Chlorella , Microalgas , Contaminantes Químicos del Agua , Cobre/toxicidad , Agua Dulce , Dureza , Concentración de Iones de Hidrógeno , Metales , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidad , Zinc/toxicidadRESUMEN
Predicting the toxicity of effluent exposures, which vary in duration, composition, and concentration, poses a challenge for ecological risk assessments. Effluent discharges may frequently result in the exposure of aquatic organisms to high concentrations of mixed contaminants for short durations. In the receiving environment effluents will undergo dilution and physical or chemical processes that further reduce contaminant concentrations at varying rates. To date, most studies comparing toxicity risks of continuous and pulsed contaminant exposures have focused on individual contaminants. In this study, the toxicity to the tropical euryhaline copepod Acartia sinjiensis of two complex effluents was assessed, comparing 6- and 18-h pulses and 78-h continuous exposures. Observations of larval development success and population size were completed after a 78-h incubation period, to observe for latent effects after pulse exposures. The chemical compositions of the effluents were assessed over time and different contaminants (i.e., metals, ammonia or organics) declined at differing rates. These were characterized as either a minimal, steady, or rapid decline. Nauplii development and population after 78 h were more impacted by effluent exposures following an 18-h pulse, compared to a 6-h pulse. Based on pulse-exposure concentrations, the 50% effect concentrations (EC50) were similar for continuous and 18-h exposures but up to 3-fold greater (lower toxicity) for the shorter 6-h exposures. Time-weighted average concentrations did not accurately predict toxicity from pulse exposures of the effluents. Concentration-addition toxicity modelling using toxicity data from pulse exposures of single contaminants was useful for predicting the toxicity of chemical mixtures exposed for varying durations. Recommendations for modified approaches to assessing risks of short-term effluent discharges are discussed.
Asunto(s)
Copépodos , Contaminantes Químicos del Agua , Amoníaco , Animales , Cobre/toxicidad , Metales/toxicidad , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidadRESUMEN
Toxicity risk assessments of short-term discharges of contaminated waters to the aquatic environment have shown that receptor organisms can tolerate higher pulse-exposure than continuous-exposure concentrations of some contaminants. However, these observations are influenced by the mode of toxicity of the contaminants present and the concentration-time profile of the exposure. For common metal contaminants, the time-weighted average concentration (TAC) of the exposure has been useful for predicting risk of toxicity to multiple species, including the tropical, euryhaline copepod Acartia sinjiensis. To increase our understanding of the application and limitations of the TAC approach, the present study examined how varied pulse-exposure durations affect the toxicity of fast-acting contaminants, ammonia, and the common pesticide propoxur to this copepod species. Copepod larvae were exposed under continuous-exposure conditions (all life stages from eggs to nauplii to copepodites exposed) and as 6- and 18-h pulse exposures applied during the most sensitive life stage only (24-h-old nauplii) within 78-h tests. Larval development ratio and population size were assessed as test endpoints. Generally, increased exposure duration resulted in increased toxicity. Trends observed for ammonia and propoxur were slightly different for larval development and population size. Larvae tolerated greater concentrations of contaminants in a 6-h pulse (higher 10% effect concentration) than in an 18-h pulse, or a continuous 78-h exposure, whereas toxicity responses converged for the 18- and 78-h exposures. Continuous toxicity thresholds were always protective of pulse exposures, providing a conservative toxicity threshold for all durations of pulse exposures. Although generalizations for predictions of risk based on TACs are frequently effective for common metal contaminants, the TAC approach was not effective for ammonia and propoxur. Environ Toxicol Chem 2022;41:208-218. © 2021 SETAC.
Asunto(s)
Copépodos , Contaminantes Químicos del Agua , Amoníaco/toxicidad , Animales , Larva , Metales/toxicidad , Propoxur , Contaminantes Químicos del Agua/toxicidadRESUMEN
Antarctic melt streams are important ecosystems that increasingly face contaminant pressures from anthropogenic sources. Metal contaminants are often reported in the limno-terrestrial environment but their speciation is not well characterised, making environmental risk assessments difficult. This paper characterises labile metal concentrations in five melt streams and three shallow lakes around the Casey and Wilkes research stations in East Antarctica using chemical extracts and field deployments of diffusive gradients in thin-film (DGT) samplers. An acute toxicity test with field-collected Ceratadon purpeus and taxonomic identification of diatoms in melt streams were used to infer environmental risk. Copper and zinc were the most labile metals in the melt streams. DGT-labile copper concentrations were up to 3 µg Cu L-1 in melt-stream waters but not labile below the sediment-water interface. DGT-labile zinc concentrations were consistent above and below the sediment-water interface at concentrations up to 14 µg Zn L-1 in four streams, but one stream showed evidence of zinc mineralisation in the sediment with a flux to overlying and pore waters attributed to the reductive dissolution of iron and manganese oxides. Other metals, such as chromium, nickel, and lead were acid-extractable from the sediments, but not labile in pore waters or overlying waters. All streams had unique compositions of freshwater diatoms, but one had particularly reduced diversity and richness, which correlated to metal contamination and sediment physico-chemical properties such as a finer particle size. In laboratory bioassays with field-collected samples of the Antarctic moss C. purpeus, there was no change in photosynthetic efficiency following 28-d exposure to 700, 900, 1060, or 530 µg L-1 of cadmium, copper, nickel, and zinc, respectively. This study shows that microorganisms such as diatoms may be at greater risk from contaminants than mosses, and highlights the importance of geochemical factors controlling metal lability.
Asunto(s)
Monitoreo del Ambiente , Contaminantes Químicos del Agua , Regiones Antárticas , Ecosistema , Sedimentos Geológicos , Lagos , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidadRESUMEN
Salinity in the Antarctic nearshore marine environment is seasonally dynamic and climate change is driving greater variability through altered sea ice seasons, ocean evaporation rates, and increased terrestrial ice melt. The greatest salinity changes are likely to occur in the nearshore environment where elevated metal exposures from historical waste or wastewater discharge occur. How salinity changes affect metal toxicity has not yet been investigated. This study investigated the toxicity of cadmium, copper, nickel, lead, and zinc, and their equitoxic mixtures across a salinity gradient to the Antarctic marine microalga Phaeocystis antarctica. In the metal-free control exposures, algal population growth rates were significantly lower at salinities <20 PSU or >35 PSU compared to the control growth rate at 35 PSU of 0.60 ± 0.05 doublings per day and there was no growth below 10 or above 68 PSU. Salinity-induced changes to metal speciation and activity were investigated using the WHAM VII model. Percentages of free ion activity and metal-organic complexes increased at decreasing salinities while the activity of inorganic metal complexes increased with increasing salinities. Despite metal speciation and activity changes, toxicity was generally unchanged across the salinity gradient except that there was less copper toxicity and more lead toxicity than model predictions at salinities of 15 and 25 PSU and antagonistic interactions in metal-mixture treatments. In mixtures with and without copper, it was shown that copper was responsible for â¼50% of the antagonism from observed toxicity at salinities below 45 PSU. Across all treatments, using different metal fractions in toxicity models did not improve toxicity predictions compared to dissolved metal concentrations. These results provide evidence that P. antarctica is unlikely to be at a greater risk from metal contaminants as a result of salinity changes.
Asunto(s)
Haptophyta , Microalgas , Contaminantes Químicos del Agua , Plomo , Salinidad , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidadRESUMEN
Increased focus on the development and application of bioavailability-based metal water quality guideline values requires increased understanding of the influence of water chemistry on metal bioavailability and toxicity. Development of empirical models, such as multiple linear regression models, requires the assessment of the influence of individual water quality parameters as toxicity-modifying factors. The present study investigated the effect of pH on the lability and toxicity of zinc (Zn) to a tropical green microalga (Chlorella sp.). Zinc speciation and lability were explored using the Windermere Humic Aqueous Model (WHAM7), ultrafiltration, and diffusive gradients in thin films (DGT). Zinc toxicity increased significantly with increasing pH from 6.7 to 8.3, with 50% growth inhibition effect concentrations decreasing from 185 to 53 µg l-1 across the pH range. Linear relationships between DGT-labile Zn and dissolved Zn did not vary across the tested pH range, nor did the linear relationship between dissolved (<0.45 µm) and ultrafiltered (<3 kDa) Zn. Our findings show that Zn toxicity to this freshwater alga is altered as a function of pH across environmentally realistic pH ranges and that these toxicity changes could not be explained by Zn speciation and lability as measured by DGT and WHAM7. Environ Toxicol Chem 2021;40:2836-2845. © 2021 SETAC.
Asunto(s)
Chlorella , Microalgas , Contaminantes Químicos del Agua , Agua Dulce , Concentración de Iones de Hidrógeno , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidad , Zinc/toxicidadRESUMEN
Effluent discharges can potentially result in high concentrations of metals entering aquatic environments for short durations, ranging from a few hours to days. The environmental risks of such exposures are challenging to accurately assess. Risk assessment tools for effluent discharges include comparison of toxicant concentrations with guideline values and the use of direct toxicity assessments, both of which were designed to assess continuous, rather than pulse, contaminant exposures. In this study, a chronic pulse-exposure toxicity test was developed using the tropical euryhaline calanoid copepod Acartia sinjiensis. This copepod has a rapid life cycle and is highly sensitive to metal contaminants, with 50% effect concentrations (chronic EC50) for larval development of 1.7, 8.6 and 0.7 µg L-1 for copper, nickel and zinc, respectively. The toxicities of copper and nickel were assessed as a continuous exposure (78 h) and as pulses (3, 6 and 18 h) initiated at varying life stages, from egg to copepodite, and measured larval development over 78 h. Generally, 24-h old nauplii were more sensitive or of similar sensitivity to copper and nickel pulses than 48-h old nauplii. The 78-h test duration enabled observations of chronic effects following pulse exposures, which frequently occurred in the absence of acute effects. The EC50 values for pulse exposures were higher than those of continuous exposure by up to approximately 16-fold and 15-fold for copper and nickel, respectively. When metal-pulse exposure concentrations were expressed using the time-weighted averaged concentration (TAC), resultant concentration response curves were similar to those in continuous exposures to the same metal, suggesting that thresholds based on continuous exposures were also protective for pulse exposures to these metals. This research improves our understanding of the toxicity of pulse contaminant exposures and assists with developing improved approaches to for the risk assessment and regulation of short-term contaminant discharges.
Asunto(s)
Copépodos , Contaminantes Químicos del Agua , Animales , Cobre , Metales/toxicidad , Pruebas de Toxicidad Crónica , Contaminantes Químicos del Agua/toxicidadRESUMEN
There have been limited studies on the effects of toxicity-modifying factors, such as dissolved organic matter (DOM), on the toxicity of metal mixtures to aquatic biota. The present study investigated the effects of DOM concentration (low, 2.8 ± 0.1 mg C/L; high, 11 ± 1.0 mg C/L) and DOM source (predominantly terrestrial or microbial) on the chronic toxicity of copper (Cu) and nickel (Ni) binary mixtures to the green freshwater microalga Chlorella sp. This was assessed by using a full factorial design of 72-h growth inhibition bioassays. Measured algal growth rate was compared with growth predicted by the concentration addition and independent action reference models. Model predictions were based on concentrations of dissolved metals, labile metals (measured by diffusive gradients in thin films [DGT]), and calculated free metal ions (determined by the Windermere Humic Aqueous Model). Copper/Ni mixture toxicity was synergistic to Chlorella sp. in the absence of added DOM, with evidence of metal concentration-dependent toxicity at low effect concentrations. As DOM concentration increased, the mixture interaction changed from synergism to noninteraction or antagonism depending on the metal speciation method used. The DOM source had no significant effect on mixture interaction when based on dissolved and free metal ion concentrations but was significantly different when based on DGT-labile metal concentrations. Ratio-dependent mixture interaction was observed in all treatments, with increased deviation from the reference model predictions as the mixture changed from Ni- to Cu-dominated. The present study demonstrated that both DOM concentration and source can significantly change metal mixture toxicity interactions and that these interactions can be interpreted differently depending on the metal speciation method used. Environ Toxicol Chem 2021;40:1908-1918. © 2021 SETAC.
Asunto(s)
Chlorella , Contaminantes Químicos del Agua , Cobre/análisis , Cobre/toxicidad , Materia Orgánica Disuelta , Níquel/análisis , Níquel/toxicidad , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidadRESUMEN
The Southeast Asia and Melanesia region has extensive nickel (Ni)-rich lateritic regoliths formed from the tropical weathering of ultramafic rocks. As the global demand for Ni continues to rise, these lateritic regoliths are increasingly being exploited for their economic benefit. Mining of these regoliths contributes to the enrichment of coastal sediments in trace metals, especially Ni. The present study used high-throughput sequencing (metabarcoding) to determine changes in eukaryote (18s v7 recombinant DNA [rDNA] and diatom-specific subregion of the 18s v4 rDNA) and prokaryote (16s v4 rDNA) community compositions along a sediment Ni concentration gradient offshore from a large lateritized ultramafic regolith in New Caledonia (Vavouto Bay). Significant changes in the eukaryote, diatom, and prokaryote community compositions were found along the Ni concentration gradient. These changes correlated most with the dilute-acid extractable concentration of Ni in the sediments, which explained 26, 23, and 19% of the variation for eukaryote, diatom, and prokaryote community compositions, respectively. Univariate analyses showed that there was no consistent change in indices of biodiversity, evenness, or richness. Diatom richness and diversity did, however, decrease as sediment acid extractable-Ni concentrations increased. Threshold indicator taxa analysis was conducted separately for each of the 3 targeted genes to detect changes in taxa whose occurrences decreased or increased along the acid extractable-Ni concentration gradient. Based on these data, 46 mg acid extractable-Ni/kg was determined as a threshold value where sensitive species began to disappear. In the case of the estuarine sediments offshore from lateritized ultramafic regolith in New Caledonia, this is recommended as an interim threshold value until further lines of evidence can contribute to a region-specific Ni sediment quality guideline value. Environ Toxicol Chem 2021;40:1894-1907. © 2021 SETAC.
Asunto(s)
Níquel , Oligoelementos , Eucariontes/genética , Sedimentos Geológicos , Minería , Níquel/toxicidadRESUMEN
Metal contaminants in Antarctic soils are typically found around research stations which are concentrated in ice-free coastal areas. The risk of these contaminants to the Antarctic environment is not well understood, given Antarctica's unique organisms and climate. This study assessed the use of diffusive gradients in thin-films (DGT), a passive sampler that measures fluxes of labile metals from soils to porewaters, in Antarctic soils. DGT-labile measurements were compared to three chemical extractants of increasing strength including high-purity water, dilute acid (1 M HCl), and concentrated acids (3:1 v/v HNO3:HCl), to understand differences in contaminant geochemistry that may affect environmental risk. One site had high lead concentrations measured with dilute (114 ± 4 mg kg-1) and concentrated (150 ± 10 mg kg-1) acids, while DGT-labile concentrations were below the method detection limit (0.5 µg L-1), indicating that the lead species has low solubility or lability. Another site had low concentrations of zinc measured by dilute (36.2 ± 0.5 mg kg-1) or concentrated (76 ± 6 mg kg-1) acid extracts, but had high DGT-labile concentrations (350 ± 80 µg L-1). This reflects an active source of zinc supplied from soil to pore water over time. Copper was found to be acid extractable, water-soluble, and DGT-labile, with DGT-labile concentrations of up to 12 µg L-1. Despite the soil and metal-specific geochemical differences, any of the extracts could be used with statistical clustering techniques to identify differences in sites with elevated metal concentrations. This study shows that the DGT-method can identify contaminated sites comparably to chemical extracts but provides environmentally relevant measurements of metal contaminant lability in Antarctic soils.
Asunto(s)
Contaminantes del Suelo , Suelo , Regiones Antárticas , Monitoreo del Ambiente , Contaminantes del Suelo/análisis , Zinc/análisisRESUMEN
Nickel laterite ore deposits are becoming increasingly important sources of Ni for the global marketplace and are found mainly in tropical and subtropical regions, including Indonesia, the Philippines, Papua New Guinea, Cuba, and New Caledonia. There are few legislatively derived standards or guidelines for the protection of aquatic life for Ni in many of these tropical regions, and bioavailability-based environmental risk assessment (ERA) approaches for metals have mainly been developed and tested in temperate regions, such as the United States and Europe. This paper reports on a multi-institutional, 5-y testing program to evaluate Ni exposure, effects, and risk characterization in the Southeast Asia and Melanesia (SEAM) region, which includes New Caledonia, Papua New Guinea, the Philippines, and Indonesia. Further, we have developed an approach to determine if the individual components of classical ERA, including effects assessments, exposure assessments, and risk characterization methodologies (which include bioavailability normalization), are applicable in this region. A main conclusion of this research program is that although ecosystems and exposures may be different in tropical systems, ERA paradigms are constant. A large chronic ecotoxicity data set for Ni is now available for tropical species, and the data developed suggest that tropical ecosystems are not uniquely sensitive to Ni exposure; hence, scientific support exists for combining tropical and temperate data sets to develop tropical environmental quality standards (EQSs). The generic tropical database and tropical exposure scenarios generated can be used as a starting point to examine the unique biotic and abiotic characteristics of specific tropical ecosystems in the SEAM region. Integr Environ Assess Manag 2021;17:802-813. © 2021 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).
Asunto(s)
Níquel , Contaminantes Químicos del Agua , Asia Sudoriental , Disponibilidad Biológica , Ecosistema , Europa (Continente) , Agua Dulce , Melanesia , Medición de Riesgo , Contaminantes Químicos del Agua/análisisRESUMEN
The geographical shift of nickel mining to small island countries of the Southeast Asia and Melanesia region has produced a need to assess the environmental risk associated with increased sediment nickel exposure to benthic estuarine/marine biota. Chemical measurements of nickel concentration and potential bioavailability, including the use of diffusive gradients in thin films (DGT), were compared to effects on 10-d reproduction of the epibenthic estuarine/marine amphipod Melita plumulosa in nickel-spiked sediments and field-contaminated sediments with different characteristics. The 10% effect concentrations (EC10s) for amphipod reproduction ranged from 280 to 690 mg/kg total recoverable nickel, from 110 to 380 mg/kg dilute acid-extractable nickel, and from 34 to 87 µg Ni/m2 /h DGT-labile nickel flux. Nickel bioavailability was lower in sediments with greater total organic carbon, clay content, and percentage of fine particles. Measurements of DGT-labile nickel flux at the sediment-water interface integrated exposure to nickel from porewater, overlying water, and ingested sediment exposure pathways and were found to have the strongest relationship with the biological response. At most, there was a 29% reduction in 10-d M. plumulosa reproduction relative to the control when exposed to nickel from field-contaminated sediments collected from nickel laterite mining regions of New Caledonia. The DGT technique can be used as a complementary tool to measure the bioavailability of nickel in estuarine/marine sediments, especially sediments that are in nickel laterite mining regions where there are no or few toxicity data available for determining biological effects on local species. Based on the combined data set of the 3 nickel-spiked sediments a DGT-labile nickel EC10 threshold of 50 (30-69) µg Ni/m2 /h was determined. Environ Toxicol Chem 2021;40:1266-1278. © 2020 SETAC.
Asunto(s)
Anfípodos , Contaminantes Químicos del Agua , Animales , Monitoreo del Ambiente , Sedimentos Geológicos , Metales/análisis , Níquel/toxicidad , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidadRESUMEN
Nickel is often a metal of interest in regulatory settings given its increasing prevalence in disturbed freshwaters and as a known toxicant to fish and algae. Dissolved organic matter (DOM) is a toxicity modifying factor for nickel and a ubiquitous water physicochemical parameter. This study investigated the effect of DOM concentration and source on the chronic toxicity of nickel to Chlorella sp. using three DOM at two concentrations (3.1 ± 1.8 and 12 ± 1.3 mg C/L). Nickel toxicity to Chlorella sp. was not strongly influenced by DOM concentration. In the absence of DOM, the 72-h EC50 for Chlorella sp. was 120 µg Ni/L. In the low DOM treatment, nickel toxicity was either unchanged or slightly increased (87-140 µg Ni/L) and unchanged or slightly decreased in the high DOM treatment (130-240 µg Ni/L). DOM source also had little effect on nickel toxicity, the largest differences in nickel toxicity occurring in the high DOM treatment. Labile nickel (measured by diffusive gradients in thin-films, DGT) followed strong linear relationships with dissolved nickel (R2 > 0.97). DOM concentration and source had limited effect on DGT-labile nickel. DGT-labile nickel decreased with increasing DOM concentration for only one of the three DOM. Modelled labile nickel concentrations (expressed as maximum dynamic concentrations, cdynmax) largely agreed with DGT-labile nickel and suggested that toxicity is explained by free Ni2+ concentrations. This study confirms that nickel toxicity is largely unaffected by DOM concentration or source and that both measured (DGT) and modelled (cdynmax and free Ni2+) nickel concentrations can explain nickel toxicity.
Asunto(s)
Chlorella , Contaminantes Químicos del Agua , Animales , Agua Dulce , Metales , Níquel/análisis , Níquel/toxicidad , Contaminantes Químicos del Agua/análisis , Contaminantes Químicos del Agua/toxicidadRESUMEN
Australian tropical freshwaters can experience extreme seasonal variability in rainfall and run off, particularly due to pulse events such as storms and cyclones. This study investigated how seasonal variability in dissolved organic matter (DOM) quality impacted the chronic toxicity of copper to a tropical green alga (Chlorella sp.) in the presence of two concentrations of DOM (low: â¼2 mg C/L; high: â¼10 mg C/L) collected from three tropical waters. Copper speciation and lability were explored using diffusive gradients in thin-films (DGT) and modelled maximum dynamic concentrations (cdynmax) using data derived from the Windermere Humic Aqueous Model (WHAM VII). Relationships between copper lability and copper toxicity were assessed as potential tools for predicting toxicity. Copper toxicity varied significantly with DOM concentration, source and season. Copper toxicity decreased with increasing concentrations of DOM, with 50% growth inhibition effect concentrations (EC50) increasing from 1.9 µg Cu/L in synthetic test waters with no added DOM (0.34 mg C/L) up to 63 µg Cu/L at DOM concentrations of 9.9 mg C/L. Copper toxicity varied by up to 2-fold between the three DOM sources and EC50 values were generally lower in the presence of wet season DOM compared to dry season DOM. Linear relationships between DGT-labile copper and dissolved copper were significantly different between DOM source, but not concentration or season. Modelled cdynmax consistently under-predicted labile copper in high DOM treatments compared to DGT measurements but performed better in low DOM treatments, indicating that this method is DOM-concentration dependent. Neither speciation method was a good surrogate for copper toxicity in the presence of different sources of natural DOM. Our findings show that DOM source and season, not just DOM concentration, affect copper toxicity to freshwater biota. Therefore, DOM quality should be considered as a toxicity-modifying factor for future derivation of bioavailability-based site-specific water quality guideline values.
Asunto(s)
Chlorella , Microalgas , Contaminantes Químicos del Agua/análisis , Australia , Cobre/análisis , Agua Dulce , Estaciones del AñoRESUMEN
Intensification of lateritic nickel mining in Southeast Asia and Melanesia potentially threatens coastal ecosystems from increased exposure to nickel and suspended sediment. This study investigated the response of Acropora muricata when exposed to either dissolved nickel, clean suspended sediment or nickel-contaminated suspended sediment for 7 days, followed by a 7-d recovery period. Significant bleaching and accumulation of nickel in coral tissue was observed only after exposure to high dissolved nickel concentrations and nickel-spiked suspended sediment. No effect on A. muricata was observed from exposure to a particulate-bound nickel concentration of 60 mg/kg acid-extractable nickel at a suspended sediment concentration of 30 mg/L TSS. This study demonstrates that bioavailability of nickel associated with suspended sediment exposure plays a key role in influencing nickel toxicity to corals. These findings assist in assessments of risk posed by increasing nickel mining activities on tropical marine ecosystems.