RESUMEN
BACKGROUND: The explosion in biological information creates the need for databases that are easy to develop, easy to maintain and can be easily manipulated by annotators who are most likely to be biologists. However, deployment of scalable and extensible databases is not an easy task and generally requires substantial expertise in database development. RESULTS: BioBuilder is a Zope-based software tool that was developed to facilitate intuitive creation of protein databases. Protein data can be entered and annotated through web forms along with the flexibility to add customized annotation features to protein entries. A built-in review system permits a global team of scientists to coordinate their annotation efforts. We have already used BioBuilder to develop Human Protein Reference Database http://www.hprd.org, a comprehensive annotated repository of the human proteome. The data can be exported in the extensible markup language (XML) format, which is rapidly becoming as the standard format for data exchange. CONCLUSIONS: As the proteomic data for several organisms begins to accumulate, BioBuilder will prove to be an invaluable platform for functional annotation and development of customizable protein centric databases. BioBuilder is open source and is available under the terms of LGPL.
Asunto(s)
Sistemas de Administración de Bases de Datos , Bases de Datos de Proteínas , Proteínas/fisiología , Programas Informáticos , Biología Computacional/métodos , Biología Computacional/normas , Bases de Datos de Proteínas/normas , Humanos , Internet , Proteínas/normas , Programas Informáticos/normas , Diseño de SoftwareRESUMEN
The rapid pace at which genomic and proteomic data is being generated necessitates the development of tools and resources for managing data that allow integration of information from disparate sources. The Human Protein Reference Database (http://www.hprd.org) is a web-based resource based on open source technologies for protein information about several aspects of human proteins including protein-protein interactions, post-translational modifications, enzyme-substrate relationships and disease associations. This information was derived manually by a critical reading of the published literature by expert biologists and through bioinformatics analyses of the protein sequence. This database will assist in biomedical discoveries by serving as a resource of genomic and proteomic information and providing an integrated view of sequence, structure, function and protein networks in health and disease.
Asunto(s)
Bases de Datos de Proteínas , Proteínas/metabolismo , Proteómica , Biología Computacional , Enfermedad , Genómica , Humanos , Almacenamiento y Recuperación de la Información , Internet , Unión Proteica , Procesamiento Proteico-Postraduccional , Proteínas/química , Proteínas/genética , Proteoma/química , Proteoma/genética , Proteoma/metabolismo , Especificidad por Sustrato , Vocabulario ControladoRESUMEN
Human Protein Reference Database (HPRD) is an object database that integrates a wealth of information relevant to the function of human proteins in health and disease. Data pertaining to thousands of protein-protein interactions, posttranslational modifications, enzyme/substrate relationships, disease associations, tissue expression, and subcellular localization were extracted from the literature for a nonredundant set of 2750 human proteins. Almost all the information was obtained manually by biologists who read and interpreted >300,000 published articles during the annotation process. This database, which has an intuitive query interface allowing easy access to all the features of proteins, was built by using open source technologies and will be freely available at http://www.hprd.org to the academic community. This unified bioinformatics platform will be useful in cataloging and mining the large number of proteomic interactions and alterations that will be discovered in the postgenomic era.
Asunto(s)
Bases de Datos de Proteínas/tendencias , Proteína BRCA1/fisiología , Biología Computacional/métodos , Genética Médica/métodos , Humanos , Sustancias Macromoleculares , Mapeo de Interacción de Proteínas/tendencias , Procesamiento Proteico-Postraduccional/fisiología , Estructura Cuaternaria de Proteína/fisiología , Estructura Terciaria de Proteína/fisiología , Especificidad por Sustrato/fisiologíaRESUMEN
The use of high-throughput DNA sequencing and proteomic methods has led to an unprecedented increase in the amount of genomic and proteomic data. Application of computing technologies and development of computational tools to analyze and present these data has not kept pace with the accumulation of information. Here, we discuss the use of different database systems to store biological information and mention some of the key emerging computing technologies that are likely to have a key role in the future of bioinformatics.
