Observation of heat pumping effect by radiative shuttling.

Heat shuttling phenomenon is characterized by the presence of a non-zero heat flow between two bodies without net thermal bias on average. It was initially predicted in the context of nonlinear heat conduction within atomic lattices coupled to two time-oscillating thermostats. Recent theoretical works revealed an analog of this effect for heat exchanges mediated by thermal photons between two solids having a temperature dependent emissivity. In this paper, we present the experimental proof of this effect using systems made with composite materials based on phase change materials. By periodically modulating the temperature of one of two solids we report that the system akin to heat pumping with a controllable heat flow direction. Additionally, we demonstrate the effectiveness of a simultaneous modulation of two temperatures to control both the strength and direction of heat shuttling by exploiting the phase delay between these temperatures. These results show that this effect is promising for an active thermal management of solid-state technology, to cool down solids, to insulate them from their background or to amplify heat exchanges.

Fatty acid metabolism-related lncRNA prognostic signature for serous ovarian carcinoma.

Background: To explore the role of fatty acid metabolism (FAM)-related lncRNAs in the prognosis and antitumor immunity of serous ovarian cancer (SOC). Materials & methods: A SOC FAM-related lncRNA risk model was developed and evaluated by a series of analyses. Additional immune-related analyses were performed to further assess the associations between immune state, tumor microenvironment and the prognostic risk model. Results: Five lncRNAs associated with the FAM genes were found and used to create a predictive risk model. The patients with a low-risk profile exhibited favorable prognostic outcomes. Conclusion: The established prognostic risk model exhibits better predictive capabilities for the prognosis of patients with SOC and offers novel potential therapy targets for SOC.

Novel Information-Driven Smoothing Spline Linearization Method for High-Precision Displacement Sensors Based on Information Criterions.

A noise-resistant linearization model that reveals the true nonlinearity of the sensor is essential for retrieving accurate physical displacement from the signals captured by sensing electronics. In this paper, we propose a novel information-driven smoothing spline linearization method, which innovatively integrates one new and three standard information criterions into a smoothing spline for the high-precision displacement sensors' linearization. Using theoretical analysis and Monte Carlo simulation, the proposed linearization method is demonstrated to outperform traditional polynomial and spline linearization methods for high-precision displacement sensors with a low noise to range ratio in the 10-5 level. Validation experiments were carried out on two different types of displacement sensors to benchmark the performance of the proposed method compared to the polynomial models and the the non-smoothing cubic spline. The results show that the proposed method with the new modified Akaike Information Criterion stands out compared to the other linearization methods and can improve the residual nonlinearity by over 50% compared to the standard polynomial model. After being linearized via the proposed method, the residual nonlinearities reach as low as ±0.0311% F.S. (Full Scale of Range), for the 1.5 mm range chromatic confocal displacement sensor, and ±0.0047% F.S., for the 100 mm range laser triangulation displacement sensor.

A Fiber-Based Chromatic Dispersion Probe for Simultaneous Measurement of Dual-Axis Absolute and Relative Displacement.

This paper presents a fiber-based chromatic dispersion probe for the simultaneous measurement of dual-axis absolute and relative displacement with nanometric resolutions. The proposed chromatic dispersion probe is based on optical dispersion. In the probe, the employed light beam is split into two sub-beams, and then the two sub-beams are made to pass through two optical paths with different optical settings where two identical single-mode fiber detectors are located at different defocused positions of the respective dispersive lenses. In this way, two spectral signals can be obtained to indicate the absolute displacement of each of the dual-axes. A signal processing algorithm is proposed to generate a normalized output wavelength that indicates the relative displacement of the dual-axis. With the proposed chromatic dispersion probe, the absolute and relative displacement measurements of the dual-axis can be realized simultaneously. Theoretical and experimental investigations reveal that the developed chromatic dispersion probe realizes an absolute measurement range and a measurement resolution of approximately 180 µm and 50 nm, respectively, for each axis. Moreover, a relative displacement measurement range and a measurement resolution of about 240 µm and 100 nm, respectively, are achieved for the dual-axis.

3D printing of multi-metallic microstructures by meniscus-confined electrodeposition.

We studied a multi-metallic microscale 3D printing based on the meniscus-confined electrodeposition (MCED). The composition of Cu/Pt alloys can be controlled by applying different bias voltages to the CuSO4/H2PtCl4 mixed solution in MCED. We find that a double-barrel system had higher Cu/Pt alloy purity (maximum 100% Cu or maximum 80% Pt) than a single-barrel system. A Λ-shaped microstructure was printed to verify the capability to multi-metal microstructures in a single printing process.

Significant loss of soil inorganic carbon at the continental scale.

Widespread soil acidification due to atmospheric acid deposition and agricultural fertilization may greatly accelerate soil carbonate dissolution and CO2 release. However, to date, few studies have addressed these processes. Here, we use meta-analysis and nationwide-survey datasets to investigate changes in soil inorganic carbon (SIC) stocks in China. We observe an overall decrease in SIC stocks in topsoil (0-30 cm) (11.33 g C m-2 yr-1) from the 1980s to the 2010s. Total SIC stocks have decreased by ∼8.99 ± 2.24% (1.37 ± 0.37 Pg C). The average SIC losses across China (0.046 Pg C yr-1) and in cropland (0.016 Pg C yr-1) account for ∼17.6%-24.0% of the terrestrial C sink and 57.1% of the soil organic carbon sink in cropland, respectively. Nitrogen deposition and climate change have profound influences on SIC cycling. We estimate that ∼19.12%-19.47% of SIC stocks will be further lost by 2100. The consumption of SIC may offset a large portion of global efforts aimed at ecosystem carbon sequestration, which emphasizes the importance of achieving a better understanding of the indirect coupling mechanisms of nitrogen and carbon cycling and of effective countermeasures to minimize SIC loss.

A Semisolid Micromechanical Beam Steering System Based on Micrometa-Lens Arrays.

Optical beam steerers have been widely employed for information acquisitions. Numerous beam steering schemes have been developed, and each of them can satisfy practical requirements for certain scenarios. However, there is still a lack of a comprehensive approach that is able to balance all of the critical technical parameters for wide range of applications. Here, a semisolid micromechanical beam steering system based on micrometa-lens arrays (MMLAs) is demonstrated. It is operated by manipulating the probe beam over two sets of decentered MMLAs potentially driven by high-speed piezo-electric motors. Small f-numbers, well-corrected aberration, and easy lateral reproduction of micrometa-lenses optimize the overall technical parameters. As a proof-of-concept, we implement such a device exhibiting diffraction-limited resolution within a large field of view of 30° × 30°. A three-dimensional depth sensing is also performed to demonstrate its potential in light detection and ranging applications.

Methyltransferase like 7B is a potential therapeutic target for reversing EGFR-TKIs resistance in lung adenocarcinoma.

BACKGROUND: Identification of potential novel targets for reversing resistance to Epidermal Growth Factor Receptor (EGFR)-tyrosine kinase inhibitors (EGFR-TKIs) holds great promise for the treatment of relapsed lung adenocarcinoma (LUAD). In the present study, we aim to investigate the role of methyltransferase-like 7B (METTL7B) in inducing EGFR-TKIs resistance in LUAD and whether it could be a therapeutic target for reversing the resistance. METHODS: METTL7B-overexpressed LUAD cell lines, gefitinib and osimertinib-resistant Cell-Derived tumor Xenograft (CDX) and Patient-Derived tumor Xenograft (PDX) mouse models were employed to evaluate the role of METTL7B in TKIs resistance. Ultraperformance liquid chromatography-tandem mass spectrometer (UPLC-MS/MS) was used to identify the metabolites regulated by METTL7B. Methylated RNA immunoprecipitation (MeRIP)-qPCR analysis was performed to measure the N6-methyladenosine (m6A) status of mRNA of METTL7B targeted genes. Gold nanocluster-assisted delivery of siRNA targeting METTL7B (GNC-siMETTL7B) was applied to evaluate the effect of METTL7B in TKIs resistance. RESULTS: Increased expression of METTL7B was found in TKIs-resistant LUAD cells and overexpression of METTL7B in LUAD cells induced TKIs resistance both in vitro and in vivo. Activated ROS-metabolism was identified in METTL7B-overexpressed LUAD cells, accompanied with upregulated protein level of GPX4, HMOX1 and SOD1 and their enzymatic activities. Globally elevated m6A levels were found in METTL7B-overexpressed LUAD cells, which was reduced by knock-down of METTL7B. METTL7B induced m6A modification of GPX4, HMOX1 and SOD1 mRNA. Knock-down of METTL7B by siRNA re-sensitized LUAD cells to gefitinib and osimertinib both in vitro and in vivo. CONCLUSIONS: This study uncovered a new critical link in METTL7B, glutathione metabolism and drug resistance. Our findings demonstrated that METTL7B inhibitors could be used for reversing TKIs resistance in LUAD patients.

A Fiber-Based Chromatic Dispersion Probe for Simultaneous Measurement of X-Axis and Z-Axis Displacements with Nanometric Resolutions.

In this paper, a fiber-based chromatic dispersion probe for simultaneous measurement of X-axis and Z-axis displacements with nanometric resolutions by using the full width at half maxima (FWHM) of the detected spectral signal has been proposed and demonstrated. For X-axis, FWHM is employed for indicating the X-axis displacement based on the fact that the FWHM remains almost constant with the varying Z-axis displacement of the fiber detector and shows a linear relationship with the X-axis displacement within a specific Z-axis displacement range. For the Z-axis, the linear relationship between the centroid wavelength λ of the detected spectral signal and the Z-axis displacement is employed for indicating the Z-axis displacement based on the fact that the sensitivity (slope of the λ-Z curve) is also linear with X-axis displacement within a certain X-axis displacement range. Theoretical and experimental investigations have verified the feasibility of the proposed chromatic dispersion probe, which yields X- and Z-axis measurement ranges of 2.3 µm and 15 µm and X- and Z-axis measurement resolutions of better than 25 nm and 50 nm, respectively. Experiments were further performed to evaluate the basic performance of the prototype probe and the maximum measurement errors were less than 10 nm and 60 nm for X- and Z-axis displacements, respectively.

All-optical laser-ultrasonic technology for width and depth gauging of rectangular surface-breaking defects.

In this paper, the width and depth of rectangular surface-breaking defects were successfully gauged using an all-optical laser-ultrasonic system. The finite element method was used to simulate propagating paths of defect-induced reflected and transmitted Rayleigh waves. It is observed that most Rayleigh waves with wavelengths less than the depth of the defect are reflected by the defect. A small part of the Rayleigh waves with wavelengths greater than the depth of the defect directly transmits through the bottom edge of the defect without acting on the left edge of the defect. Based on the simulation results, a three-step detection method of width and depth gauging of rectangular surface-breaking defects is proposed. In the first step, the pulsed laser and detection laser are irradiated on one side of the defect. In the second step, the sample is moved to a certain distance to ensure that both lasers reach the other side of the defect. In the third step, two lasers are irradiated on both sides of the defect. The width and depth of the defect are calculated according to the arrival time of the incident, reflected, and transmitted Rayleigh waves, as well as the movement distance of the sample. Experimental results are consistent with the reference-results measured by using a digital microscope. The proposed three-step detection method is proved to be feasible in simultaneous measurement of the width and depth of rectangular surface-breaking defects. Furthermore, it may be potentially useful for measuring other types of defect structures.

Model for liver hardness using two-dimensional shear wave elastography, durometer, and preoperative biomarkers.

BACKGROUND: Post-hepatectomy liver failure (PHLF) increases morbidity and mortality after liver resection for patients with advanced liver fibrosis and cirrhosis. Preoperative liver stiffness using two-dimensional shear wave elastography (2D-SWE) is widely used to evaluate the degree of fibrosis. However, the 2D-SWE results were not accurate. A durometer measures hardness by quantifying the ability of a material to locally resist the intrusion of hard objects into its surface. However, the durometer score can only be obtained during surgery. AIM: To measure correlations among 2D-SWE, palpation by surgeons, and durometer-measured objective liver hardness and to construct a liver hardness regression model. METHODS: We enrolled 74 hepatectomy patients with liver hardness in a derivation cohort. Tactile-based liver hardness scores (0-100) were determined through palpation of the liver tissue by surgeons. Additionally, liver hardness was measured using a durometer. Correlation coefficients for durometer-measured hardness and preoperative parameters were calculated. Multiple linear regression models were constructed to select the best predictive durometer scale. Receiver operating characteristic (ROC) curves and univariate and multivariate analyses were used to calculate the best model's prediction of PHLF and risk factors for PHLF, respectively. A separate validation cohort (n = 162) was used to evaluate the model. RESULTS: The stiffness measured using 2D-SWE and palpation scale had good linear correlation with durometer-measured hardness (Pearson rank correlation coefficient 0.704 and 0.729, respectively, P < 0.001). The best model for the durometer scale (hardness scale model) was based on stiffness, hepatitis B virus surface antigen, and albumin level and had an R 2 value of 0.580. The area under the ROC for the durometer and hardness scale for PHLF prediction were 0.807 (P = 0.002) and 0.785 (P = 0.005), respectively. The optimal cutoff value of the durometer and hardness scale was 27.38 (sensitivity = 0.900, specificity = 0.660) and 27.87 (sensitivity = 0.700, specificity = 0.787), respectively. Patients with a hardness scale score of > 27.87 were at a significantly higher risk of PHLF with hazard ratios of 7.835 (P = 0.015). The model's PHLF predictive ability was confirmed in the validation cohort. CONCLUSION: Liver stiffness assessed by 2D-SWE and palpation correlated well with durometer hardness values. The multiple linear regression model predicted durometer hardness values and PHLF.

Parametric study on interply tracking in multilayer composites by analytic-signal technology.

Recently, researchers proposed the use of ultrasound combined with analytic-signal concepts for the reconstruction of the internal ply structure of composites. Optimal parameters for the pulse-echo mode ultrasonic testing are determined by modeling the analytic-signal response. The internal structure can be reconstructed by instantaneous metrics based on the interaction of the multilayer structure and the ultrasonic wave. However, there are certain drawbacks associated with the use of instantaneous metrics. The phase-derived interply track tends to be sensitive to the inspection conditions. This paper analytically studies the errors of the interply tracking for a wide range of parameters, including (i) signal-to-noise ratio, (ii) bandwidth, (iii) interply thickness, and (iv) attenuation, amongst others. It provides a guideline on how to improve the performance of the interply tracking procedure in real measurements. An experimental study combining the analytic-signal procedure with a standard log-Gabor filter in the frequency domain is performed to derive the interply tracks of a 24-layer composite laminate in a robust way. The bandpass filter selects the appropriate frequency band of the analytic-signal response from the composite. It shows a good ability for frequency and bandwidth selection, and can efficiently cope with noise features. The reconstructed ply tracks in A-scan, B-scan, and C-scan modes are analyzed to verify the performance of this procedure.

Chromatically multi-focal optics based on micro-lens array design.

CCD arrays encode color information via uniformly distributed red, green and blue pixels. Therefore, even a perfectly achromatic system projecting an image onto a CCD plane cannot possibly associate a single object point with the 3 or more discrete pixels encoding color content. Here, we propose and demonstrate a micro-lens array (MLA) design that simultaneously corrects chromatic aberrations and separates color channels to spatially distinct pixels. Starting from a commercially available aspheric condenser lens, methods to design and assess the performance of a few microns deep MLA etched on the convex optical surface are detailed. Actual fabrication is carried out by fluid jet polishing, with an optical form deviation of 0.24 µm rms. Finally, the MLA is assessed with a narrowly collimated beam containing two wavelengths, which produces distinct spots of diameter 10-15 µm as expected.

Prophylactic intra-aortic balloon pump in patients with left main disease undergoing off-pump coronary artery bypass grafting.

BACKGROUND: Preventive intra-aortic balloon pump (IABP) for high-risk patients with stable hemodynamics is controversial, and its definition of high-risk is still unclear. This study aimed to investigate the effect of prophylactic IABP on the early outcome of left main disease (LMD) patients receiving off-pump coronary artery bypass grafting (OPCABG) with stable hemodynamics. METHODS: From January 2013 to April 2020, 257 consecutive patients who underwent OPCABG through sternotomy were enrolled in this study. All LMD patients (greater than 70%) had stable hemodynamics (BP>100 mmHg without vasoconstrictor substance infusion). Early outcomes of 125 patients with prophylactic IABP (IABP group) and 132 patients without IABP (Control group) were compared in this study. RESULTS: IABP did not show favorable effect on the conversion to CPB (RR 0.63, 95%CI 0.05-7.89, P = 0.7211), perioperative MI (RR 0.69, 95%CI 0.22-2.12, P = 0.5163), mortality (RR 0.65, 95%CI 0.04-10.25, P = 0.7608) or the composite end of the conversion, MI and mortality (RR 0.63, 95%CI 0.23-1.74, P = 0.3747). There was greater incidence of prolonged ventilation in IABP after adjustment (RR2.16, 95%CI 1.12-4.18, P = 0.0221). There was no IABP-related mortality or limb ischemia. CONCLUSION: No significant difference in early outcomes was observed in hemodynamically stable patients with LMD between prophylactic IABP group and control group. Prophylactic IABP may be unnecessary in patients with LMD undergoing OPCABG.

Ultra-stable Biomembrane Force Probe for Accurately Determining Slow Dissociation Kinetics of PD-1 Blockade Antibodies on Single Living Cells.

Immune checkpoint blockade with monoclonal antibodies (mAbs) that target programmed cell death protein-1 (PD-1) has remarkably revolutionized cancer therapy. Their binding kinetics measured by surface plasmon resonance does not always correlate well with their immunotherapeutic efficacies, mainly due to the lack of two-dimensional cell plasma membrane and the capability of force sensing and manipulation. In this regard, based on a more suitable and ultra-sensitive biomechanical nanotool, biomembrane force probe (BFP), we developed a Double-edge Smart Feedback control system as an ultra-stable platform to characterize ultra-long bond lifetimes of receptor-ligand binding on living cells. We further benchmarked the dissociation kinetics for three clinically approved PD-1 blockade mAbs (Nivolumab, Pembrolizumab, and Camrelizumab), intriguingly correlating well with the objective response rates in the hepatocellular carcinoma second-line treatment. This ultra-stable BFP potentially provides a compelling kinetic platform to direct the screening, optimization, and clinical selection of therapeutic antibodies in the future.

A new method for detecting surface defects on curved reflective optics using normalized reflectivity.

Detection of surface defects is critical in quality control of reflective optics. In this note, we propose a new surface defect detection method for reflective optics using the normalized reflectivity, which is calculated from the signal intensity of a chromatic confocal surface profiler. This detection method first scans the surface to acquire signal intensity data and then models the intensity data to calculate the normalized local reflectivity map. The reflectivity map is further processed by threshold segmentation to extract defects from normal areas. Measurement experiments on an Al-coated concave reflector with artificial defects were carried out to demonstrate the feasibility of the method. This detection method can provide existing optical surface profilers with defect detecting capabilities without extra equipment.

Simultaneous ultrasonic parameter estimation of a multi-layered material by the PSO-based least squares algorithm using the reflection spectrum.

Advanced multi-layered materials with superior performance are required for many applications. The non-destructive characterization of multi-layer properties is a hot spot of current research. The least squares inversion method using the reflection spectrum has been developed and widely used to estimate the properties of thin single layers simultaneously. However this method has the problems of a loss in speed and simplicity, and a local optimal solution, especially in the cases of a multi-layered structure because of the increasing estimated parameters and the uncertainty influence from the parameters. Particle swarm optimization (PSO) is a robust global search algorithm similar to 'bird' foraging, which can be used to improve the performance of the least squares inversion algorithm. This paper has proposed a PSO-based least squares estimation using the ultrasonic reflection spectrum to make simultaneous measurement. The simulation and experiment, carried out on the aluminum-TC4 bi-layered material, tested and proved the capability of the new algorithm. The real measured parameters and the estimated parameters were obtained. The results have been compared to analyze the errors of the estimated parameters.

A rotary scanning method to evaluate grooves and porosity for nerve guide conduits based on ultrasound microscopy.

Grooved nerve guide conduits (NGCs) have been effective in the clinical treatment of peripheral nerve injury. They are generally fabricated from a micro-structured spinneret using a spinning process, which easily can cause a variety of pores and morphological deviation. The topography of internal grooves as well as the porosity can greatly influence the therapeutic effect. Traditional optical or scanning electron microscopy (SEM) methods can be used to image the grooves; however, these methods are destructive and require slicing NGCs to prepare specimens suitable for imaging. Moreover, lengthy experiments and large batches of NGCs are required to ensure reliable results from both in vitro experiments and clinical studies. In this paper, a non-destructive method for evaluating the grooves and porosity of NGCs is proposed using ultrasonic imaging combined with rotary scanning and an image analysis algorithm. Two ultrasonic methods were used: a 25-MHz point-focus ultrasonic transducer applied to observe axial cross sections of the conduits and a 100-MHz point-focus ultrasonic transducer to detect large pores caused by defects. Furthermore, a theoretical algorithm for detecting the local porosity of a conduit based on density is proposed. Herein, the proposed acoustic method and traditional optical methods are evaluated and compared. A parameter representing the specific surface area of the internal grooves is introduced and computed for both the optical and acoustic methods, and the relative errors of the computed parameter values for three different NGCs were 7.0%, 7.9%, and 15.3%. The detected location and shape of pores were consistent between the acoustic and optical methods, and greater porosity was observed in the middle of the conduit wall. In this paper, the results of the acoustic and optical methods are presented and the errors relating to the acoustic factors, device characteristics, and image processing method are further analyzed.

Numerical and experimental analysis of a focused reflected wave in a multi-layered material based on a ray model.

A focal probe is used for the acoustic measurement of a thin layer of a material with unknown sound velocity. This is now possible, because an algorithm, based on the focused ray model, has been found. However, there are still several problems such as the assumption that the half-aperture angle equals the incident angle, the identification of the longitudinal-wave focus, and the composition of the signal. In this work, we study the multi-mode wave focus numerically and experimentally to identify the focused longitudinal waves. A theoretical multilayered focusing model has been introduced based on geometrical acoustics. In addition, a phase differentiation theory is proposed to find the incident angle for the focus of the tilted rays, which is referred to as maximum half-aperture angle in other studies. The V(z,t) curve of a single layer, with a thickness of 1.5 mm and 2.0 mm, and a multi-layer are obtained using vertical translational movement. Both thickness and sound velocity are derived from the curve simultaneously. Our single layer experiments show that it is possible to focus multimode waves. The single and multi-layer experiments confirm the multi-layered focused ray model and phase differentiation theory. Furthermore, experiments are conducted to analyze the measured results.