RESUMEN
We have examined the seroprevalence of BDV in wild Japanese macaques (Macaca fuscata) in the peninsula (Chiba prefecture), Japan. Serum samples from macaques were examined by the ELISA, Western blot and immunofluorescence assays to detect the presence of serum antibodies that react specifically to BDV antigens. Among 49 investigated individuals, 6 (12.2%) showed positive reaction to BDV antigens. RT-PCR studies detected BDV sequences in brain tissue of one case among four seropositive cases examined. Sequence analysis revealed a high degree of genetic conservation between BDV sequences derived from Japanese macaques and those documented for other animal species. Nevertheless, phylogenetic analysis revealed unique differences between macaque and other species derived BDV sequences.
Asunto(s)
Virus de la Enfermedad de Borna/genética , Macaca/virología , Filogenia , ARN Viral/aislamiento & purificación , Animales , Secuencia de Bases , Western Blotting , Análisis por Conglomerados , Cartilla de ADN , Ensayo de Inmunoadsorción Enzimática , Técnica del Anticuerpo Fluorescente , Japón , Datos de Secuencia Molecular , ARN Viral/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Alineación de Secuencia , Análisis de Secuencia de ADN , Estudios SeroepidemiológicosRESUMEN
A global positioning system (GPS) collar recorded the locations of an adult female Japanese macaque over a 9-day period in a habitat with mixed suburban and rural land-uses in Chiba Prefecture, Japan. The GPS device acquired positions even in forested areas. The GPS data located the female mostly in forested areas, although the female had ranged through a habitat with inter-mingled fields, orchards, quarries, and residential areas. However, the GPS position acquisition rate was low compared to studies carried out on North American mammals. The GPS fixed a position in 20% of positioning attempts. When the collared female was tracked by radio-telemetry, almost all failures of the GPS to fix a position occurred in forest.
