Enhancing strawberry resilience to saline, alkaline, and combined stresses with light spectra: impacts on growth, enzymatic activity, nutrient uptake, and osmotic regulation.

BACKGROUND: This study examines the effects of various complementary light spectra on the growth, development, antioxidant activity, and nutrient absorption in strawberry plants under stress conditions. Light-emitting diodes (LEDs) were used to provide specific wavelengths, including monochromatic blue (460 nm), monochromatic red (660 nm), a dichromatic mix of blue and red (1:3 ratio), full-spectrum white light (400-700 nm), and ambient light as a control (no LED treatment). The stress treatments applied were: control (no stress), salinity (80 mM NaCl), alkalinity (40 mM NaHCO3), and a combined salinity/alkalinity condition. RESULTS: Our results indicated that complementary light spectra, especially red and blue/red, helped mitigate the adverse effects of stress on plant growth and development. These spectra improved plant tolerance by enhancing the activity of polyphenol oxidase and peroxidase enzymes and increasing starch accumulation in the leaves. Furthermore, under stress conditions, red and blue-red light significantly boosted fruit anthocyanin levels. Although stress elevated antioxidant activity, supplementary light reduced this activity by alleviating stress compared to ambient light. While stress led to increased Na and Cl ion concentrations in leaves, treatments with blue, red, and blue-red light minimized these harmful effects and promoted the absorption of beneficial ions such as K, Mg, Fe, and Cu. CONCLUSIONS: Adjusting light quality significantly influences the morphology and physiology of strawberry plants, underscoring the role of specific light spectra in promoting optimal growth under stress conditions. CLINICAL TRIAL NUMBER: Not applicable.

Photosynthetic Performance and Yield Losses of Winter Rapeseed (Brassica napus L. var. napus) Caused by Simulated Hail.

Winter oilseed rape (Brassica napus L.), Europe's foremost oilseed crop, is significantly impacted by hailstorms, leading to substantial yield reductions that are difficult to predict and measure using conventional methods. This research aimed to assess the effectiveness of photosynthetic efficiency analysis for predicting yield loss in winter rapeseed subjected to hail exposure. The aim was to pinpoint the chlorophyll fluorescence parameters most affected by hail stress and identify those that could act as non-invasive biomarkers of yield loss. The study was conducted in partially controlled conditions (greenhouse). Stress was induced in the plants by firing plastic balls with a 6 mm diameter at them using a pneumatic device, which launched the projectiles at speeds of several tens of meters per second. Measurements of both continuous-excitation and pulse-modulated-amplitude chlorophyll fluorescence were engaged to highlight the sensitivity of the induction curve and related parameters to hail stress. Our research uncovered that some parameters such as Fs, Fm', ΦPSII, ETR, Fo, Fv/Fm, and Fv/Fo measured eight days after the application of stress had a strong correlation with final yield, thus laying the groundwork for the creation of new practical protocols in agriculture and the insurance industry to accurately forecast damage to rapeseed crops due to hail stress.

Improving strawberry plant resilience to salinity and alkalinity through the use of diverse spectra of supplemental lighting.

BACKGROUND: This study explores the impact of various light spectra on the photosynthetic performance of strawberry plants subjected to salinity, alkalinity, and combined salinity/alkalinity stress. We employed supplemental lighting through Light-emitting Diodes (LEDs) with specific wavelengths: monochromatic blue (460 nm), monochromatic red (660 nm), dichromatic blue/red (1:3 ratio), and white/yellow (400-700 nm), all at an intensity of 200 µmol m-2 S-1. Additionally, a control group (ambient light) without LED treatment was included in the study. The tested experimental variants were: optimal growth conditions (control), alkalinity (40 mM NaHCO3), salinity (80 mM NaCl), and a combination of salinity/alkalinity. RESULTS: The results revealed a notable decrease in photosynthetic efficiency under both salinity and alkalinity stresses, especially when these stresses were combined, in comparison to the no-stress condition. However, the application of supplemental lighting, particularly with the red and blue/red spectra, mitigated the adverse effects of stress. The imposed stress conditions had a detrimental impact on both gas exchange parameters and photosynthetic efficiency of the plants. In contrast, treatments involving blue, red, and blue/red light exhibited a beneficial effect on photosynthetic efficiency compared to other lighting conditions. Further analysis of JIP-test parameters confirmed that these specific light treatments significantly ameliorated the stress impacts. CONCLUSIONS: In summary, the utilization of blue, red, and blue/red light spectra has the potential to enhance plant resilience in the face of salinity and alkalinity stresses. This discovery presents a promising strategy for cultivating plants in anticipation of future challenging environmental conditions.

Risk assessment of hollow-bearing trees in urban forests.

The paper is a study of risk assessment posed by trees in selected urban woodlands (urban forests) of Warsaw. Two groups of trees were analysed and compared: exhibiting signs of maturity and ageing (hollow-bearing trees with open or hidden cavities and/or caries) and with no signs of decay. 373 individual trees growing near routes frequently or continuously used for recreational purposes were examined using Roloff's vitality classification, and tree risk assessment method, complemented by instrumental studies: a resistance resistograph, pulling tests, and sonic tomography (SoT). The collected data was analysed using the Chi-square test. The results indicate that it is not possible to conclude unequivocally that the presence of hollows in aged trees significantly increases the risk of falling. According to the safety factor results from the SoT and pulling tests, no correlation was demonstrated between the presence of hollow trees and an increase in risk class. The highest proportion of hollow trees (89.42%) was in the low risk group for trunk fracture and uprooting. The results also indicate the coherence of the diagnostic methods to be necessary for providing sufficient information to assess the statics and, ultimately, as our study showed, the protection of hollow trees.

Genotype-dependent Strategies to "Overcome" Excessive Light: Insights into Non-Photochemical Quenching under High Light Intensity.

High light (HL) intensities have a significant impact on energy flux and distribution within photosynthetic apparatus. To understand the effect of high light intensity (HL) on the HL tolerance mechanisms in tomatoes, we examined the response of the photosynthesis apparatus of 12 tomato genotypes to HL. A reduced electron transfer per reaction center (ET0 /RC), an increased energy dissipation (DI0 /RC) and non-photochemical quenching (NPQ), along with a reduced maximum quantum yield of photosystem II (FV /FM ), and performance index per absorbed photon (PIABS ) were common HL-induced responses among genotypes; however, the magnitude of those responses was highly genotype-dependent. Tolerant and sensitive genotypes were distinguished based on chlorophyll fluorescence and energy-quenching responses to HL. Tolerant genotypes alleviated excess light through energy-dependent quenching (qE ), resulting in smaller photoinhibitory quenching (qI ) compared to sensitive genotypes. Quantum yield components also shifted under HL, favoring the quantum yield of NPQ (ՓNPQ ) and the quantum yield of basal energy loss (ՓN0 ), while reducing the efficient quantum yield of PSII (ՓPSII ). The impact of HL on tolerant genotypes was less pronounced. While the energy partitioning ratio did not differ significantly between sensitive and tolerant genotypes, the ratio of NPQ components, especially qI , affected plant resilience against HL. These findings provide insights into different patterns of HL-induced NPQ components in tolerant and sensitive genotypes, aiding the development of resilient crops for heterogeneous light conditions.

Sunlight-induced repair of photosystem II in moss Semibarbula orientalis under submergence stress.

Lower plants such as bryophytes often encounter submergence stress, even in low precipitation conditions. Our study aimed to understand the mechanism of submergence tolerance to withstand this frequent stress in moss (Semibarbula orientalis ) during the day and at night. These findings emphasise that light plays a crucial role in photoreactivation of PSII in S. orientalis , which indicates that light not only fuels photosynthesis but also aids in repairing the photosynthetic machinery in plants. Submergence negatively affects photosynthesis parameters such as specific and phenomenological fluxes, density of functional PSII reaction centres (RC/CS), photochemical and non-photochemical quenching (Kp and Kn), quantum yields (ϕP0 , ϕE0 , ϕD0 ), primary and secondary photochemistry, performance indices (PIcs and PIabs), etc. Excessive antenna size caused photoinhibition at the PSII acceptor side, reducing the plastoquinone pool through the formation of PSII triplets and reactive oxygen species (ROS). This ROS-induced protein and PSII damage triggered the initiation of the repair cycle in presence of sunlight, eventually leading to the resumption of PSII activity. However, ROS production was regulated by antioxidants like superoxide dismutase (SOD) and catalase (CAT) activity. The rapid recovery of RS/CS observed specifically under sunlight conditions emphasises the vital role of light in enabling the assembly of essential units, such as the D1 protein of PSII, during stress in S. orientalis . Overall, light is instrumental in restoring the photosynthetic potential in S. orientalis growing under submergence stress. Additionally, it was observed that plants subjected to submergence stress during daylight hours rapidly recover their photosynthetic performance. However, submergence stress during the night requires a comparatively longer period for the restoration of photosynthesis in the moss S. orientalis .

Exogenous epibrassinolide application improves essential oil biosynthesis and trichome development in peppermint via modulating growth and physicochemical processes.

Peppermint has gained a promising status due to the presence of a high proportion of bioactive compounds, especially menthol. Due to its pharmacological efficacy, the demand for its plant-based bioactive compounds necessitates its cultivation worldwide. Brassinosteroids are polyhydroxylated sterol derivatives that regulate diverse processes and control many agronomic traits during plant growth and development. A factorial randomised pot experiment was performed in the net house to investigate the effect of 24-Epibrassinolide (EBL) on the growth, physiology, essential oil content, stomatal behaviour and trichome development of the three cultivars of peppermint. Four levels of foliage-applied EBL, viz. 0, 10-5, 10-6 and 10-7 M were applied to the three cultivars of peppermint (Kukrail, Pranjal and Tushar). Among the different treatments of EBL, the application of 10-6 M increased shoot length by 38.84, 37.59 and 36.91%, root length by 36.73, 29.44 and 33.47%, chlorophyll content by 24.20, 22.48 and 23.32%, PN by 32.88, 32.61 and 33.61%, EO content by 32.72, 30.00 and 28.84%, EO yield per plant by 66.66, 77.77 and 73.33% and menthol yield per plant by 127.27, 110 and 118.18% in Kukrail, Tushar and Pranjal respectively, compared with their respective control plants. Further, the 10-6 M EBL exhibited improved trichome size and density, cellular viability and menthol content of the oil analysed from scanning electron microscopy, confocal laser scanning microscopy and GC-MS respectively as compared to the control. In conclusion, out of different levels of EBL, two sprays of 10-6 M EBL proved effective in enhancing the morphophysiological features and productivity of mint plants, particularly for cultivar Kukrail.

Titanium dioxide nanoparticles require K+ and hydrogen sulfide to regulate nitrogen and carbohydrate metabolism during adaptive response to drought and nickel stress in cucumber.

Crop plants face severe yield losses worldwide owing to their exposure to multiple abiotic stresses. The study described here, was conducted to comprehend the response of cucumber seedlings to drought (induced by 15% w/v polyethylene glycol 8000; PEG) and nickel (Ni) stress in presence or absence of titanium dioxide nanoparticle (nTiO2). In addition, it was also investigated how nitrogen (N) and carbohydrate metabolism, as well as the defense system, are affected by endogenous potassium (K+) and hydrogen sulfide (H2S). Cucumber seedlings were subjected to Ni stress and drought, which led to oxidative stress and triggered the defense system. Under the stress, N and carbohydrate metabolism were differentially affected. Supplementation of the stressed seedlings with nTiO2 (15 mg L-1) enhanced the activity of antioxidant enzymes, ascorbate-glutathione (AsA-GSH) system and elevated N and carbohydrates metabolism. Application of nTiO2 also enhanced the accumulation of phytochelatins and activity of the enzymes of glyoxalase system that provided additional protection against the metal and toxic methylglyoxal. Osmotic stress brought on by PEG and Ni, was countered by the increase of proline and carbohydrates levels, which helped the seedlings keep their optimal level of hydration. Application nTiO2 improved the biosynthesis of H2S and K+ retention through regulating Cys biosynthesis and H+-ATPase activity, respectively. Observed outcomes lead to the conclusion that nTiO2 maintains redox homeostasis, and normal functioning of N and carbohydrates metabolism that resulted in the protection of cucumber seedlings against drought and Ni stress. Use of 20 mM tetraethylammonium chloride (K+- channel blocker), 500 µM sodium orthovanadate (PM H+-ATPase inhibitor), and 1 mM hypotaurine (H2S scavenger) demonstrate that endogenous K+ and H2S were crucial for the nTiO2-induced modulation of plants' adaptive responses to the imposed stress.

Griseofulvin Inhibits Root Growth by Targeting Microtubule-Associated Proteins Rather Tubulins in Arabidopsis.

Griseofulvin was considered an effective agent for cancer therapy in past decades. Although the negative effects of griseofulvin on microtubule stability are known, the exact target and mechanism of action in plants remain unclear. Here, we used trifluralin, a well-known herbicide targeting microtubules, as a reference and revealed the differences in root tip morphology, reactive oxygen species production (ROS), microtubule dynamics, and transcriptome analysis between Arabidopsis treated with griseofulvin and trifluralin to elucidate the mechanism of root growth inhibition by griseofulvin. Like trifluralin, griseofulvin inhibited root growth and caused significant swelling of the root tip due to cell death induced by ROS. However, the presence of griseofulvin and trifluralin caused cell swelling in the transition zone (TZ) and meristematic zone (MZ) of root tips, respectively. Further observations revealed that griseofulvin first destroyed cortical microtubules in the cells of the TZ and early elongation zone (EZ) and then gradually affected the cells of other zones. The first target of trifluralin is the microtubules in the root MZ cells. Transcriptome analysis showed that griseofulvin mainly affected the expression of microtubule-associated protein (MAP) genes rather than tubulin genes, whereas trifluralin significantly suppressed the expression of αß-tubulin genes. Finally, it was proposed that griseofulvin could first reduce the expression of MAP genes, meanwhile increasing the expression of auxin and ethylene-related genes to disrupt microtubule alignment in root tip TZ and early EZ cells, induce dramatic ROS production, and cause severe cell death, eventually leading to cell swelling in the corresponding zones and inhibition of root growth.

GO nanoparticles mitigate the negative effects of salt and alkalinity stress by enhancing gas exchange and photosynthetic efficiency of strawberry plants.

Considering the potential use of nanomaterials, particularly carbon-based nanostructures, in agriculture, we conducted a study to investigate the effect of graphene oxide (GO) on strawberry plants under salinity and alkalinity stress conditions. We used GO concentrations of 0, 2.5, 5, 10, and 50 mg/L, and applied stress treatments at three levels: without stress, salinity (80 mM NaCl), and alkalinity (40 mM NaHCO3). Our results indicate that both salinity and alkalinity stress negatively impacted the gas exchange parameters of the strawberry plants. However, the application of GO significantly improved these parameters. Specifically, GO increased PI, Fv, Fm, and RE0/RC parameters, as well as chlorophyll and carotenoid contents in the plants. Moreover, the use of GO significantly increased the early yield and dry weight of leaves and roots. Therefore, it can be concluded that the application of GO can enhance the photosynthetic performance of strawberry plants, and improve their resistance to stress conditions.

Photosynthetic efficiency of perennial ryegrass (Lolium perenne L.) seedlings in response to Ni and Cd stress.

Perennial ryegrass is a grass species used to establish lawns in urban areas where pollution is a major environmental problems. Cadmium (Cd) and nickel (Ni) contribute significantly to these pollutants and may cause photosynthetic limitation. The main objective of this work was to perform a comprehensive analysis of photosynthetic efficiency of perennial ryegrass seedlings under Cd and Ni stress. Some of the main indices of photosynthetic efficiency (prompt and delayed chlorophyll-a fluorescence signals and modulated reflectance at 820 nm) were compared with growth parameters. Two cultivars were tested: 'Niga' and 'Nira'. A decrease in photosystem (PS) II and PSI activity was observed. This was due to an increase in nonradiative dissipation of the PSII antenna, a decrease in PSII antenna size, or a decrease in the number of photosynthetic complexes with fully closed PSII RCs. Efficiency of electron transport was decreased. The effect on the modulated reflectance signal could indicate a restriction in electron flow from PSII to PSI. The correlation between photosynthetic efficiency parameters, such as Area, Fo, Fm, and Fv, and growth parameters, confirmed that some photosynthetic efficiency parameters can be used as indicators for early detection of heavy metal effects.

Comparative physiological and biochemical mechanisms in diploid, triploid, and tetraploid watermelon (Citrullus lanatus L.) grafted by branches.

Seed production for polyploid watermelons is costly, complex, and labor-intensive. Tetraploid and triploid plants produce fewer seeds/fruit, and triploid embryos have a harder seed coat and are generally weaker than diploid seeds. In this study, we propagated tetraploid and triploid watermelons by grafting cuttings onto gourd rootstock (C. maxima × C. mochata). We used three different scions: the apical meristem (AM), one-node (1N), and two-node (2N) branches of diploid, triploid, and tetraploid watermelon plants. We then evaluated the effects of grafting on plant survival, some biochemical traits, oxidants, antioxidants, and hormone levels at different time points. We found significant differences between the polyploid watermelons when the 1N was used as a scion. Tetraploid watermelons had the highest survival rates and the highest levels of hormones, carbohydrates, and antioxidant activity compared to diploid watermelons, which may explain the high compatibility of tetraploid watermelons and the deterioration of the graft zone in diploid watermelons. Our results show that hormone production and enzyme activity with high carbohydrate content, particularly in the 2-3 days after transplantation, contribute to a high survival rate. Sugar application resulted in increased carbohydrate accumulation in the grafted combination. This study also presents an alternative and cost-effective approach to producing more tetraploid and triploid watermelon plants for breeding and seed production by using branches as sprouts.

Salicylic acid and trehalose attenuate salt toxicity in Brassica juncea L. by activating the stress defense mechanism.

Two significant soil degradation processes that pose a hazard to our ecosystems are soil salinization and sodification. The information on potential of salicylic acid (SA) and trehalose (Tre) to induce abiotic stress signaling and triggers physio-biochemical responses in crop plants is limited. Therefore, the present study was aimed to investigate the efficacy of 5 µM SA and/or 10 mM Tre in improving the growth, photosynthesis, ion homeostasis, nutrient acquisition, antioxidant defense system and yield of mustard plants growing under sodium chloride (NaCl) stress (0, 50, 100 and 150 mM NaCl). The data showed that increasing NaCl stress concentration decreased growth, photosynthesis, membrane permeability, ion homeostasis and yield in a dose-dependent manner while increasing considerably enzymatic antioxidant enzyme activities, compatible solute accumulation, sodium ion and oxidative stress biomarkers linearly with increasing NaCl stress concentration. The spray of SA, Tre, and SA + Tre played diversified roles in enhancing NaCl stress tolerance in mustard at morpho-physiological and biochemical levels. The combined SA + Tre application proved best and completely neutralized the NaCl stress-induced suppression in growth, photosynthesis, ion homeostasis, nutrient acquisition and yield by significantly enhancing the activities of enzymatic antioxidants, compatible solutes accumulation, water status and membrane permeability, while reducing considerably osmotic stress, reactive oxygen species generation, lipid peroxidation, cell death and sodium uptake in mustard. The SA + Tre application enhanced relative water content by 23%, net photosynthetic rate by 48%, superoxide dismutase activity by 51% and seed yield per plant by 64%, while decreased superoxide anion content by 26%, sodium ion content by 36% and malondialdehyde content by 25% over 0 mM NaCl treatment. Our findings indicate that the co-application of SA + Tre can be a suitable approach to palliate the ill effect of NaCl stress in mustard plants.

Comparing the salinity tolerance of twenty different wheat genotypes on the basis of their physiological and biochemical parameters under NaCl stress.

The climate has drastically changed over the past two decades. Rising temperatures and climate change may lead to increased evapotranspiration, specifically soil evaporation, causing water to evaporate and salt to accumulate in the soil, resulting in increased soil salinity. As a result, there is a need to evaluate methods for predicting and monitoring the effects of salinity on crop growth and production through rapid screening. Our study was conducted on 20 wheat genotypes, 10 sensitive and 10 tolerant, exposed to two salinity levels (90 and 120 mM NaCl) with the control under greenhouse conditions. Our results revealed significant differences in the genotypes' response to salinity. Salt stress decreased chlorophyll index in sensitive genotypes but increased chlorophyll a and carotenoids in tolerant genotypes at 90 mM. Salt stress also increased protein, proline, lipoxygenase, and reactive thiobarbituric acid levels in all wheat genotypes. The study suggests that plant photosynthetic efficiency is a reliable, non-destructive biomarker for determining the salt tolerance of wheat genotypes, while other biochemical traits are destructive and time-consuming and therefore not suitable for rapid screening.

Effects of Mycotoxin Fumagillin, Mevastatin, Radicicol, and Wortmannin on Photosynthesis of Chlamydomonas reinhardtii.

Mycotoxins are one of the most important sources for the discovery of new pesticides and drugs because of their chemical structural diversity and fascinating bioactivity as well as unique novel targets. Here, the effects of four mycotoxins, fumagillin, mevastatin, radicicol, and wortmannin, on photosynthesis were investigated to identify their precise sites of action on the photosynthetic apparatus of Chlamydomonas reinhardtii. Our results showed that these four mycotoxins have multiple targets, acting mainly on photosystem II (PSII). Their mode of action is similar to that of diuron, inhibiting electron flow beyond the primary quinone electron acceptor (QA) by binding to the secondary quinone electron acceptor (QB) site of the D1 protein, thereby affecting photosynthesis. The results of PSII oxygen evolution rate and chlorophyll (Chl) a fluorescence imaging suggested that fumagillin strongly inhibited overall PSII activity; the other three toxins also exhibited a negative influence at the high concentration. Chl a fluorescence kinetics and the JIP test showed that the inhibition of electron transport beyond QA was the most significant feature of the four mycotoxins. Fumagillin decreased the rate of O2 evolution by interrupting electron transfer on the PSII acceptor side, and had multiple negative effects on the primary photochemical reaction and PSII antenna size. Mevastatin caused a decrease in photosynthetic activity, mainly due to the inhibition of electron transport. Both radicicol and wortmannin decreased photosynthetic efficiency, mainly by inhibiting the electron transport efficiency of the PSII acceptor side and the activity of the PSII reaction centers. In addition, radicicol reduced the primary photochemical reaction efficiency and antenna size. The simulated molecular model of the four mycotoxins' binding to C. reinhardtii D1 protein indicated that the residue D1-Phe265 is their common site at the QB site. This is a novel target site different from those of commercial PSII herbicides. Thus, the interesting effects of the four mycotoxins on PSII suggested that they provide new ideas for the design of novel and efficient herbicide molecules.

Potential hazard characteristics of trees with hollows, cavities and fruiting bodies growing along pedestrian routes.

This article is a study of risk assessment of trees with hollows, cavities and fruiting bodies for the improvement of the management and protection of urban trees growing along pedestrian routes. 317 trees were examined using TRAQ risk classes, VTA and ISA BMP methodology, Roloff's vitality classification, and sonic tomography (SoT) during the spring and summer of 2021. The collected data was analysed using the Kruskal-Wallis H-test, the Dunn multiple comparison test, the pairwise comparison of proportions with Holm correction, the U-Manna-Whitney test, and the Fisher exact test. The analysed trees grow alongside public footpaths and footways in central Zakopane, Poland. The study results indicate that tree trunk hollows are judged to have no adverse effects on a tree's vitality when assessed using visual methods and are deemed to have a limited effect on vitality estimated with SoT. Though most high and moderate-risk trees, according to SoT (88% and 80%, respectively), had hollows, such trees were a small fraction of all 171 trees with hollows, cavities and/or fruiting bodies, 2.3% and 8.8%, respectively. Therefore, the decision to remove a tree should be based on advice from a professional arborist, supported by sonic tomography (SoT) or similar objective methods.

Interactive effect of elevated CO2 and drought on physiological traits of Datura stramonium.

Rising atmospheric CO2 concentrations are known to influence the response of many plants under drought. This paper aimed to measure the leaf gas exchange, water use efficiency, carboxylation efficiency, and photosystem II (PS II) activity of Datura stramonium under progressive drought conditions, along with ambient conditions of 400 ppm (aCO2) and elevated conditions of 700 ppm (eCO2). Plants of D. stramonium were grown at 400 ppm and 700 ppm under 100 and 60% field capacity in a laboratory growth chamber. For 10 days at two-day intervals, photosynthesis rate, stomatal conductance, transpiration rate, intercellular CO2 concentration, water use efficiency, intrinsic water use efficiency, instantaneous carboxylation efficiency, PSII activity, electron transport rate, and photochemical quenching were measured. While drought stress had generally negative effects on the aforementioned physiological traits of D. stramonium, it was found that eCO2 concentration mitigated the adverse effects of drought and most of the physiological parameters were sustained with increasing drought duration when compared to that with aCO2. D. stramonium, which was grown under drought conditions, was re-watered on day 8 and indicated a partial recovery in all the parameters except maximum fluorescence, with this recovery being higher with eCO2 compared to aCO2. These results suggest that elevated CO2 mitigates the adverse growth effects of drought, thereby enhancing the adaptive mechanism of this weed by improving its water use efficiency. It is concluded that this weed has the potential to take advantage of climate change by increasing its competitiveness with other plants in drought-prone areas, suggesting that it could expand into new localities.

Enhancement of photosynthesis efficiency and yield of strawberry (Fragaria ananassa Duch.) plants via LED systems.

Due to advances in the industrial development of light-emitting diodes (LEDs), much research has been conducted in recent years to get a better understanding of how plants respond to these light sources. This study investigated the effects of different LED-based light regimes on strawberry plant development and performance. The photosynthetic pigment content, biochemical constituents, and growth characteristics of strawberry plants were investigated using a combination of different light intensities (150, 200, and 250 µmol m-2 s-1), qualities (red, green, and blue LEDs), and photoperiods (14/10 h, 16/8 h, and 12/12 h light/dark cycles) compared to the same treatment with white fluorescent light. Plant height, root length, shoot fresh and dry weight, chlorophyll a, total chlorophyll/carotenoid content, and most plant yield parameters were highest when illuminated with LM7 [intensity (250 µmol m-2 s-1) + quality (70% red/30% blue LED light combination) + photoperiod (16/8 h light/dark cycles)]. The best results for the effective quantum yield of PSII photochemistry Y(II), photochemical quenching coefficient (qP), and electron transport ratio (ETR) were obtained with LM8 illumination [intensity (250 µmol m-2 s-1) + quality (50% red/20% green/30% blue LED light combination) + photoperiod (12 h/12 h light/dark cycles)]. We conclude that strawberry plants require prolonged and high light intensities with a high red-light component for maximum performance and biomass production.

Structure-based ligand design and discovery of novel tenuazonic acid derivatives with high herbicidal activity.

INTRODUCTION: Computer-aided design has become an important tool to develop novel pesticides based on natural lead compounds. Tenuazonic acid (TeA), a typical representative of the natural tetramic acid family, was patented as a potential bioherbicide. However, its herbicidal efficacy is still not up to the ideal standard of commercial products. OBJECTIVES: We aim to find new TeA's derivatives with improved potency. METHODS: Molecular docking was used to build ligand-acceptor interaction models, design and screen new derivatives. Phytotoxicity, oxygen evolution rate, chlorophyll fluorescence and herbicidal efficacy were determined to estimate biological activity of compounds. RESULTS: With the aid of a constructed molecular model of natural lead molecule TeA binding to the QB site in Arabidopsis D1 protein, a series of derivatives differing in the alkyl side chain were designed and ranked according to free energies. All compounds are stabilized by hydrogen bonding interactions between their carbonyl oxygen O2 and D1-Gly256 residue; moreover, hydrogen bond distance is the most important factor for maintaining high binding affinity. Among 54 newly designed derivatives, D6, D13 and D27 with better affinities than TeA were screened out and synthesized to evaluate their photosynthetic inhibitory activity and herbicidal efficacy. Analysis of structure-activity relationship indicated that D6 and D13 with sec-pentyl and sec-hexyl side chains, respectively, were about twice more inhibitory of PSII activity and effective as herbicide than TeA with a sec-butyl side chain. CONCLUSION: D6 and D13 are promising compounds to develop TeA-derived novel PSII herbicides with superior performance.