RESUMEN
Chromosome 5 abnormalities, deletion of the long arm of chromosome 5 (del(5q)) or monosomy 5 (-5), arise in about 10% of myelodysplastic syndromes (MDS), either as the sole cytogenetic abnormality or as part of complicated karyotype, and has distinct clinical implications for MDS. However, the prognostic factors of MDS patients with chromosome 5 abnormalities are not determined yet. In this study, 183 Japanese MDS patients with chromosome 5 abnormalities were analyzed. Estimated incidence of del(5q) and 5q- syndrome among MDS patients was 8.4 and 1.3%, respectively. Significant shorter overall survival (OS) and leukemia-free survival (LFS) were observed in -5 patients than del(5q) patients. Among del(5q) patients, addition of monosomy 7 or complex karyotype with more than three abnormalities were significantly related to shorter OS. LFS of del(5q) patients was divided into two risk groups by international prognostic scoring system (IPSS): low/intermediate (Int)-1 and Int-2/high groups. LFS sorted by World Health Organization classification-based prognostic scoring system (WPSS) was also divided into two groups: very low/low/Int and high/very high, and WPSS was able to predict the outcome of del(5q) patients more clearly than IPSS. Together with additional cytogenetic data, WPSS might be useful for clinical decision making in MDS patients with del(5q).
Asunto(s)
Deleción Cromosómica , Cromosomas Humanos Par 5 , Monosomía , Síndromes Mielodisplásicos/genética , Anciano , Femenino , Humanos , Japón , Masculino , Síndromes Mielodisplásicos/mortalidad , Neutropenia/etiología , Pronóstico , Modelos de Riesgos ProporcionalesRESUMEN
AIMS: Diffuse large B-cell lymphoma (DLBCL) usually proliferates effacing lymph follicles. In occasional cases, tumour cells show an interfollicular pattern of proliferation preserving lymph follicles. The aim was to analyse clinicopathological findings in DLBCL showing an interfollicular pattern of proliferation to determine whether this type of lymphoma is a distinct entity of DLBCL. METHODS AND RESULTS: Clinicopathological findings in 12 cases of DLBCL showing an interfollicular pattern of proliferation [interfollicular group (IF)] were examined and compared with those in 30 cases of DLBCL with ordinary morphology [control group (CG)]. IF showed a significantly lower lactate dehydrogenase level and International Prognostic Index scores than CG (P = 0.023 and P < 0.01, respectively). The frequency of localized disease, clinical stage 1 and 2, in IF was higher than that in CG (P = 0.016). A morphologically polymorphous pattern of proliferation was found in seven of 12 cases (58.3%) in IF, which was higher than that in CG, five (16.7%) of 30 cases (P < 0.01). Clonality analysis with the polymerase chain reaction method revealed that all 11 IF cases examined showed a monoclonal pattern. Immunohistochemically, the majority (11 of 12) of IF cases showed a non-germinal centre B-cell phenotype and the frequency was higher than that in CG (P = 0.021). CONCLUSION: Diffuse large B-cell lymphoma with an interfollicular pattern of proliferation shows distinct clinical and pathological findings from ordinary DLBCL.
Asunto(s)
Linfoma de Células B Grandes Difuso/patología , Adulto , Anciano , Anciano de 80 o más Años , Estudios de Casos y Controles , Proliferación Celular , Femenino , Centro Germinal/citología , Humanos , Inmunohistoquímica , Japón , L-Lactato Deshidrogenasa/metabolismo , Linfoma de Células B Grandes Difuso/diagnóstico , Masculino , Persona de Mediana Edad , Fenotipo , PronósticoRESUMEN
We initially conducted a multicenter, randomized trial (n=43), and subsequently a questionnaire study (n=209) of participating hospitals, to evaluate whether infused fresh frozen plasma (FFP) could prevent the occurrence of hepatic veno-occlusive disease (VOD) after stem cell transplantation (SCT). Forty-three patients were divided into two groups: 23 receiving FFP infusions and 20 not receiving it. VOD developed in three patients not receiving FFP. Plasma von Willebrand factor (VWF) antigen levels were lower at days 0, 7 and 28 after SCT in patients receiving FFP than in those not receiving it, whereas plasma ADAMTS13 activity (ADAMTS13:AC) did not differ between them. Plasma VWF multimer (VWFM) was demonstrated to be defective in the high approximately intermediate VWFM during the early post-SCT phase, but there was a significant increase in high VWFM just before VOD onset. This suggests that a relative enzyme-to-substrate (ADAMTS13/high-VWFM) imbalance is involved in the pathogenesis of VOD. To strengthen this hypothesis, the incidence of VOD was apparently lower in patients receiving FFP infusions than in those not receiving it (0/23 vs 3/20) in the randomized trial. Further, the results combined with the subsequent questionnaire study (0/36 vs 11/173) clearly showed the incidence to be statistically significant (0/59 vs 14/193, P=0.033).
Asunto(s)
Proteínas ADAM/sangre , Enfermedad Veno-Oclusiva Hepática/prevención & control , Plasma , Trasplante de Células Madre , Factor de von Willebrand/análisis , Proteína ADAMTS13 , Adolescente , Adulto , Estudios de Casos y Controles , Niño , Preescolar , Neoplasias Hematológicas/sangre , Neoplasias Hematológicas/complicaciones , Neoplasias Hematológicas/patología , Neoplasias Hematológicas/terapia , Enfermedad Veno-Oclusiva Hepática/sangre , Enfermedad Veno-Oclusiva Hepática/etiología , Enfermedad Veno-Oclusiva Hepática/patología , Humanos , Lactante , Masculino , Persona de Mediana Edad , Plasma/enzimologíaAsunto(s)
Isoflavonas/farmacología , Melanocitos/efectos de los fármacos , Melanosomas/metabolismo , Ophiopogon/química , Células Cultivadas , Técnicas de Cocultivo , Citoesqueleto/efectos de los fármacos , Dendritas/efectos de los fármacos , Humanos , Isoflavonas/aislamiento & purificación , Queratinocitos/efectos de los fármacos , Queratinocitos/metabolismo , Melanocitos/metabolismoRESUMEN
We previously reported that all-trans retinoic acid (ATRA) inhibits growth in human T-cell leukemia virus type 1 (HTLV-1)-positive T-cell lines and fresh cells from patients with adult T-cell leukemia. However, the mechanism of this inhibition is not clear. In the present study, we observed that NF-kappaB transcriptional activity as well as cell growth decreased significantly in HTLV-1-positive T-cell lines in the presence of ATRA. Furthermore, we observed that ATRA reduced HTLV-1 proviral DNA, HTLV-1 genes (gag, tax, or pol mRNA) using the real-time quantitative polymerase chain reaction. SIL-2R was reduced by ATRA in both protein level (culture supernantant) and mRNA level in HTLV-1-positive T-cell lines. Interestingly, ATRA significantly inhibited RT activity similar to azidothimidine (AZT) in HTLV-1-positive T-cell lines. Moreover, AZT inhibited proviral DNA but not NF-kappaB transcriptional activity, and sIL-2R on HTLV-1; however, ATRA inhibited of NF-kappaB, proviral DNA and sIL-2R on HTLV-1. These results suggested that the decrease in sIL-2R induced by ATRA may be caused by the actions of a NF-kappaB inhibitor acting on the NF-kappaB/sIL-2R signal pathway. These results suggested that ATRA could have two roles, as a NF-kappaB inhibitor and as an RT inhibitor.
Asunto(s)
Antineoplásicos/farmacología , Leucemia de Células T/tratamiento farmacológico , Leucemia de Células T/metabolismo , Transducción de Señal/efectos de los fármacos , Tretinoina/farmacología , Adulto , División Celular/efectos de los fármacos , Infecciones por Deltaretrovirus/tratamiento farmacológico , Infecciones por Deltaretrovirus/metabolismo , Infecciones por Deltaretrovirus/fisiopatología , Regulación Leucémica de la Expresión Génica/efectos de los fármacos , Regulación Viral de la Expresión Génica/efectos de los fármacos , Productos del Gen gag/genética , Productos del Gen pol/genética , Genes pX/genética , Virus Linfotrópico T Tipo 1 Humano/genética , Virus Linfotrópico T Tipo 1 Humano/crecimiento & desarrollo , Humanos , Técnicas In Vitro , Células Jurkat , Leucemia de Células T/fisiopatología , FN-kappa B/metabolismo , Provirus/genética , Receptores de Interleucina-2/metabolismo , Solubilidad , Activación Transcripcional/efectos de los fármacos , Carga ViralRESUMEN
Corticosteroids are highly effective anti-inflammatory or immunosuppressive drugs used commonly to treat human systemic lupus erythematosus (SLE). All-trans-retinoic acid (ATRA), which belongs to a class of retinoids that exert immunomodulatory and anti-inflammatory functions, can also suppress the development of lupus nephritis in an animal model. However, both agents can inflict serious adverse effects. Here, we have asked whether ATRA can serve as a steroid-sparing drug in the treatment of lupus nephritis. To examine the efficacy of combining predonisolone (PSL) with ATRA, we treated intraperitoneally New Zealand black/white F1 (NZB/W F1) mice with PSL, ATRA or both agents. Survival rate and proteinuria were determined once a month. Cytokine and anti-DNA antibody production were determined by enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR). Renal histopathology was observed by haematoxylin and periodic acid Schiff (PAS), immunoperoxidase and immunohistochemical assay. Survival rate and proteinuria were improved in all experimental groups, and were much improved in the mice receiving the combination of ATRA and PSL (P <0.05). A single administration of ATRA reduced the Th1 [interleukin (IL)-2, interferon (IFN)-gamma and IL-12], and a Th2 (IL-4) cytokine level, as effectively as administration of PSL. ATRA also suppressed the expression of inducible nitric oxide synthetase (iNOS) and monocyte chemoattractant protein-1 (MCP-1) in the kidney. The combination of PSL and ATRA significantly reduced IgG2 (especially IgG2b)-specific anti-DNA antibody levels in comparison with administration of either agent alone. These data suggest that ATRA might have the potential to act as a new therapeutic and steroid-sparing drug against lupus nephritis.
Asunto(s)
Antiinflamatorios/uso terapéutico , Glucocorticoides/uso terapéutico , Nefritis Lúpica/tratamiento farmacológico , Prednisolona/uso terapéutico , Tretinoina/uso terapéutico , Animales , Anticuerpos Antinucleares/sangre , Peso Corporal/efectos de los fármacos , Quimiocina CCL2/inmunología , Citocinas/sangre , Citocinas/inmunología , ADN/inmunología , Quimioterapia Combinada , Femenino , Inmunoglobulina G/análisis , Inmunohistoquímica/métodos , Queratolíticos/uso terapéutico , Riñón/inmunología , Riñón/patología , Nefritis Lúpica/sangre , Nefritis Lúpica/mortalidad , Ratones , Ratones Endogámicos NZB , Óxido Nítrico Sintasa/inmunología , Óxido Nítrico Sintasa de Tipo II , Proteinuria/tratamiento farmacológico , ARN Mensajero/análisis , Bazo/efectos de los fármacosRESUMEN
The T-helper 1/T-helper 2 (Th1/Th2) cell balance was examined in 6-month-old New Zealand black/white F1 (B/WF1) mice treated with an immunosuppressive agent, FK506. The survival rate of mice treated with 10 mg/kg/day of FK506 was 7/8, while that of those treated with 2.5 mg/kg/day was 5/8, and 4/8 after treatment for 8 weeks with placebo. Proteinuria, which was already positive in all mice before the treatment, in the seven of eight mice treated with 10 mg/kg/day remained mildly positive (< or = 1+), while seven of eight mice treated with 2.5 mg/kg/day and six of eight mice treated with the placebo showed severe proteinuria (> or = 2+). Pathological changes in the kidneys of mice treated with 10 mg/kg/day of FK506 were less severe than in mice treated with the placebo or 2.5 mg/kg/day of FK506. Expression of mRNA was unchanged for all cytokines determined in the groups treated with 2.5 mg/kg/day of FK506 or placebo. In contrast, expression of mRNA for interleukin (IL)-2, and interferon (IFN)-gamma was suppressed, while that for IL-4 and IL-10 was not suppressed in the group treated with 10 mg/kg of FK506. The serum levels of IgG-class anti-DNA antibodies, which had been elevated before the treatment, were suppressed--especially in the IgG2a subclass--and the deposition of IgG2a and IgG2b in the glomeruli was reduced in the group treated with 10 mg/kg/day of FK506 compared with the other groups. These findings suggest that an improvement in the lupus nephritis of 6-month-old B/WF1 mice induced by FK506 might be associated with a predominant inhibition of Th1 cytokine.
Asunto(s)
Citocinas/efectos de los fármacos , Nefritis Lúpica/tratamiento farmacológico , Nefritis Lúpica/inmunología , Tacrolimus/farmacología , Células TH1/efectos de los fármacos , Animales , Anticuerpos Antinucleares/análisis , Anticuerpos Antinucleares/efectos de los fármacos , Secuencia de Bases , Citocinas/inmunología , Modelos Animales de Enfermedad , Relación Dosis-Respuesta a Droga , Esquema de Medicación , Femenino , Inmunoglobulina G/análisis , Inmunoglobulina G/efectos de los fármacos , Inmunohistoquímica , Inmunosupresores/farmacología , Nefritis Lúpica/patología , Recuento de Linfocitos , Masculino , Ratones , Ratones Endogámicos NZB , Datos de Secuencia Molecular , Probabilidad , Valores de Referencia , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Estadísticas no Paramétricas , Tasa de Supervivencia , Células TH1/inmunologíaAsunto(s)
Antineoplásicos/administración & dosificación , Leucemia-Linfoma de Células T del Adulto/tratamiento farmacológico , Enfermedades de la Piel/tratamiento farmacológico , Tretinoina/administración & dosificación , Adulto , Humanos , Leucemia-Linfoma de Células T del Adulto/complicaciones , Enfermedades de la Piel/etiologíaAsunto(s)
Antígenos CD34/análisis , Quimiocinas CXC/fisiología , Células Madre Hematopoyéticas/fisiología , Síndromes Mielodisplásicos/sangre , Adulto , Anciano , Apoptosis , Quimiocina CXCL12 , Quimiocinas CXC/sangre , Quimiotaxis , Femenino , Humanos , Masculino , Persona de Mediana Edad , Receptores CXCR4/análisisRESUMEN
Hemophagocytic syndrome (HPS) is a life-threatening disorder characterized by pancytopenia and activation of macrophages. Recently, corticosteroid incorporated in lipid microspheres (liposteroid) has been reported to be taken up by macrophages and to suppress their functions. Here we present a case of systemic lupus erythematosus complicated by HPS that was successfully treated with liposteroid in addition to an oral corticosteroid and intravenous high-dose cyclophosphamide therapy. The serum levels of tumor necrosis factor-alpha and ferritin that have been reported to be associated with activity of macrophages remarkably reduced after liposteroid administration. This case suggests that liposteroid is useful for the treatment of HPS.
Asunto(s)
Corticoesteroides/administración & dosificación , Ciclofosfamida/administración & dosificación , Dexametasona/administración & dosificación , Histiocitosis de Células no Langerhans/tratamiento farmacológico , Lupus Eritematoso Sistémico/tratamiento farmacológico , Administración Oral , Adulto , Biopsia con Aguja , Médula Ósea/patología , Citocinas/análisis , Quimioterapia Combinada , Femenino , Estudios de Seguimiento , Histiocitosis de Células no Langerhans/complicaciones , Histiocitosis de Células no Langerhans/diagnóstico , Humanos , Inmunohistoquímica , Interleucinas/análisis , Lupus Eritematoso Sistémico/complicaciones , Lupus Eritematoso Sistémico/diagnóstico , Índice de Severidad de la Enfermedad , Resultado del TratamientoRESUMEN
Infection with cytomegalovirus (CMV) or human herpesvirus-6 (HHV-6) may have a role in vascular endothelial damage after bone marrow transplantation (BMT). In total, 41 patients who underwent BMT were classified into four groups (12, 10, 7, and 12 patients who were infected with both CMV and HHV-6, CMV alone, HHV-6, and neither virus, respectively). Levels of thrombomodulin, plasminogen activator inhibitor-1, and cyclic GMP were 7.5+/-1.7 FU/ml, 76.4+/-24.1 ng/ml, and 9.51+/-1.1 pmol/ml, respectively, in the patients with both viruses, while the respective values were 2.9+/-0.67 FU/ml, 33.8+/-8.09 ng/ml, and 2.90+/-1.4 pmol/ml in patients infected with CMV alone, 4.8+/-0.96 FU/ml, 47.7+/-9.21 ng/ml, and 5.48+/-0.55 pmol/ml in patients with HHV-6 alone, and 1.6+/-0.39, 17.5+/-7.88 ng/ml, and 0.45+/-0.3 in those with neither virus. All three markers were significantly higher in the three groups with at least one virus than in the uninfected patients (P<0.05), and were also higher in patients with HHV-6 alone than in those with CMV alone (P<0.05). These results suggest that infection by CMV or HHV-6 causes vascular endothelial injury, with HHV-6 having a stronger effect than CMV, and combined infection having a stronger effect than either virus alone. Such viral infection may be a cause of thrombotic microangiopathy after BMT.
Asunto(s)
Trasplante de Médula Ósea/efectos adversos , Infecciones por Citomegalovirus/patología , Endotelio Vascular/patología , Endotelio Vascular/virología , Herpesvirus Humano 6 , Infecciones por Roseolovirus/patología , Aciclovir/administración & dosificación , Adolescente , Adulto , Antivirales/administración & dosificación , Infecciones por Citomegalovirus/tratamiento farmacológico , Femenino , Humanos , Masculino , Persona de Mediana Edad , Trombosis/virologíaRESUMEN
A 45-year-old woman had first been diagnosed with polymyositis because of the presence of focal necrosis, regeneration and inflammatory infiltration in the muscle fibers, and elevated creatinine phosphokinase levels. However, a pathological re-evaluation and family history led to the definite diagnosis of limb-girdle muscular dystrophy (MD). This case suggests that MD should be taken into consideration in the differential diagnosis of the inflammatory myopathies and genetic surveys including dystrophin molecule may be necessary if the condition manifests during or after adolescence, or when the family history is uninformative. In this case, the serum anti-nuclear antibody was positive, and it may represent the first time that ANA positivity has been found in limb-girdle MD.
Asunto(s)
Anticuerpos Antinucleares/sangre , Errores Diagnósticos , Distrofia Muscular de Duchenne/diagnóstico , Polimiositis/diagnóstico , Femenino , Humanos , Persona de Mediana Edad , Músculo Esquelético/patologíaAsunto(s)
Diabetes Insípida Neurogénica/etiología , Vasculitis del Sistema Nervioso Central/complicaciones , Diabetes Insípida Neurogénica/tratamiento farmacológico , Diabetes Insípida Neurogénica/patología , Femenino , Glucocorticoides/uso terapéutico , Humanos , Imagen por Resonancia Magnética , Persona de Mediana Edad , Hipófisis/patología , Prednisolona/uso terapéutico , Vasculitis del Sistema Nervioso Central/tratamiento farmacológico , Vasculitis del Sistema Nervioso Central/patologíaRESUMEN
Aspergillosis is known for the variety of unusual presentations in immuno-suppressed patients. We report a patient in whom aspergillosis caused the superior vena cava (SVC) syndrome. A 37-year-old woman became febrile soon after bone marrow transplantation (BMT). Chest radiography demonstrated a 5-cm mass extending from the right lung apex to the right supraclavicular fossa beside her Hickman catheter. She then developed SVC syndrome, which progressed despite treatment. Despite recovery of the white blood cell count, the patient continued to deteriorate, became comatose, suffered a cardiac arrest and died 31 days after BMT. Autopsy revealed Aspergillus infection at the apex of the right lung associated with innominate artery thrombosis.
Asunto(s)
Aspergilosis/complicaciones , Trasplante de Médula Ósea/efectos adversos , Síndrome de la Vena Cava Superior/microbiología , Adulto , Aspergilosis/etiología , Autopsia , Resultado Fatal , Femenino , Humanos , Leucemia/complicaciones , Leucemia/terapia , Síndrome de la Vena Cava Superior/etiología , Trasplante HomólogoRESUMEN
Cutaneous GVHD is histologically similar to eruptions induced by drugs containing a sulfhydryl group. The levels of interleukin-2 and interleukin-2 receptor were determined in a group of patients undergoing bone marrow transplantation (BMT) without graft-versus-host disease or any other complications and in a group with cutaneous graft-versus-host disease (GVHD) alone. In patients who only developed cutaneous GVHD, both interleukin-2 and inter-leukin-2 receptor levels were elevated when the disease was evident. As the elevation of these parameters became more marked, the grade of cutaneous graft versus-host disease also increased. In some patients, only one of the two parameters was elevated and the grade of graft-versus-host disease was low or no skin manifestations were seen. These findings suggest that interleukin-2 and interleukin-2 receptor act together in the development of cutaneous GVHD. This study also showed that the mechanism of cutaneous GVHD resembles that involved in the induction of eruptions by sulfhydryl-containing drugs.
Asunto(s)
Trasplante de Médula Ósea/efectos adversos , Erupciones por Medicamentos/etiología , Enfermedad Injerto contra Huésped/etiología , Enfermedades de la Piel/inducido químicamente , Compuestos de Sulfhidrilo/efectos adversos , Enfermedad Aguda , Adolescente , Adulto , Trasplante de Médula Ósea/inmunología , Estudios de Casos y Controles , Diagnóstico Diferencial , Erupciones por Medicamentos/patología , Enfermedad Injerto contra Huésped/inmunología , Enfermedad Injerto contra Huésped/patología , Humanos , Interleucina-2/sangre , Masculino , Persona de Mediana Edad , Receptores de Interleucina-2/sangre , Enfermedades de la Piel/diagnóstico , Enfermedades de la Piel/patología , Trasplante Homólogo/efectos adversos , Trasplante Homólogo/inmunologíaRESUMEN
We investigated whether pretreatment with eicosapentaenoic acid, an inhibitor of leukotriene (LT) B4, could ameliorate acute colonic graft-versus-host disease (GVHD) after bone marrow transplantation (BMT). Seventeen patients undergoing unrelated BMT were divided into two groups, with eight patients receiving eicosapentaenoic acid and nine not receiving it. The grade of GVHD after transplantation was compared with that estimated from the pretransplantation LTB4 level. The levels of LTB4 and several cytokines were also monitored. The actual grade of GVHD was lower than that estimated from LTB4 levels in three of the eight patients from the treated group, and there was a significant difference between the treated and untreated groups (p < 0.05, chi 2 test). The levels of LTB4, tumor necrosis factor-alpha (TNF-alpha), and interferon-gamma (IFN-gamma) were all significantly lower in the treated group (p < 0.05, Student's t-test). These findings suggest that eicosapentaenoic acid may ameliorate acute colonic GVHD when administered from before BMT.