Reciprocal inhibition of the thigh muscles in humans: A study using transcutaneous spinal cord stimulation.

Evaluating reciprocal inhibition of the thigh muscles is important to investigate the neural circuits of locomotor behaviors. However, measurements of reciprocal inhibition of thigh muscles using spinal reflex, such as H-reflex, have never been systematically established owing to methodological limitations. The present study aimed to clarify the existence of reciprocal inhibition in the thigh muscles using transcutaneous spinal cord stimulation (tSCS). Twenty able-bodied male individuals were enrolled. We evoked spinal reflex from the biceps femoris muscle (BF) by tSCS on the lumber posterior root. We examined whether the tSCS-evoked BF reflex was reciprocally inhibited by the following conditionings: (1) single-pulse electrical stimulation on the femoral nerve innervating the rectus femoris muscle (RF) at various inter-stimulus intervals in the resting condition; (2) voluntary contraction of the RF; and (3) vibration stimulus on the RF. The BF reflex was significantly inhibited when the conditioning electrical stimulation was delivered at 10 and 20 ms prior to tSCS, during voluntary contraction of the RF, and during vibration on the RF. These data suggested a piece of evidence of the existence of reciprocal inhibition from the RF to the BF muscle in humans and highlighted the utility of methods for evaluating reciprocal inhibition of the thigh muscles using tSCS.

Plantar Flexor Muscle Activity and Fascicle Behavior in Gastrocnemius Medialis During Running in Highly Cushioned Shoes With Carbon-Fiber Plates.

The purposes of this study were to clarify the electromyography (EMG) of plantar flexors and to analyze the fascicle and tendon behaviors of the gastrocnemius medialis (GM) during running in the carbon-fiber plate embedded in thicker midsole racing shoes, such as the Nike ZoomX Vaporfly (VF) and traditional racing shoes (TRAD). We compared the fascicle and series elastic element behavior of the GM and EMG of the lower limb muscles during running (14 km/h, 45 s) in athletes wearing VF or TRAD. GM EMGs in the push-off phase were approximately 50% lower in athletes wearing VF than in TRAD. Although the series elastic element behavior and/or mean fascicle-shortening velocity during the entire stance phase were not significantly different between VF and TRAD, a significant difference was found in both the mean EMG and integral EMG of the GM during the push-off phase. EMG of the gastrocnemius lateralis (GL) during the first half of the push-off phase was significantly different between VF and TRAD. Present results suggest that VF facilitates running propulsion, resulting in a decrease in GM and GL EMGs at a given running velocity during the push-off phase, leading to a reduction in metabolic cost.

Constraints on hand-foot coordination associated with phase dependent modulation of corticospinal excitability during motor imagery.

Interlimb coordination involving cyclical movements of hand and foot in the sagittal plane is more difficult when the limbs move in opposite directions compared with the same direction (directional constraint). Here we first investigated whether the directional constraint on hand-foot coordination exists in motor imagery (imagined motion). Participants performed 10 cyclic coordinated movements of right wrist flexion-extension and right ankle dorsiflexion-plantarflexion as quickly and precisely as possible, in the following three conditions; (1) actual movements of the two limbs, (2) imaginary movements of the two limbs, and (3) actual movement of one limb combined with imaginary movement of the other limb. Each condition was performed under two directions; the same and the opposite direction. Task execution duration was measured as the time between the first and second press of a button by the participants. The opposite directional movement took a significantly longer time than did the same directional movement, irrespective of the condition type. This suggests that directional constraint of hand-foot coordination occurs even in motor imagery without actual motor commands or kinesthetic signals. We secondarily examined whether the corticospinal excitability of wrist muscles is modulated in synchronization with an imaginary foot movement to estimate the neural basis of directional constraint on imaginary hand-foot coordination. The corticospinal excitability of the forearm extensor in resting position increased during dorsiflexion and decreased during plantarflexion similarly in both actual and imaginary foot movements. This corticospinal modulation depending on imaginary movement phase likely produces the directional constraint on the imaginary hand-foot coordination.

Interlimb and Intralimb Coordination of Rectus Femoris and Biceps Femoris Muscles at Different Running Speeds.

PURPOSE: The purpose of this study was to investigate the relationship between spatiotemporal variables and the muscle activity of the rectus femoris (RF) and biceps femoris (BF) in both legs at various running speeds. METHODS: Eighteen well-trained male athletes (age: 20.7 ± 1.8 yr) were asked to run for 50 m with 7 different "subjective efforts (SE)" (20%, 40%, 60%, 80%, 90%, 95%, and 100% SE). SE scaled relative to the maximal effort running (100%). The spatiotemporal variables (running speed, step frequency, step length) were measured over the distance from 30 to 50 m. The RF and BF muscle activities were obtained from both legs with wireless electromyography (EMG) sensors. We calculated RF and BF onset/offset timings in both legs (e.g., ipsilateral leg RF is "iRF," contralateral leg BF is "cBF"), which were expressed as % of a running cycle. Based on those timings, we obtained the EMG timing variables (%), as Switch1 (iBF offset to iRF onset), Switch2 (iRF offset to iBF onset), Scissors1 (cBF onset to iRF onset), and Scissors2 (iRF offset to cBF offset). RESULTS: running speed was well correlated with the SE, and higher running speed (>9 m·s -1 ) was achieved with higher step frequency (>4.0 Hz). Relative timings of RF and BF onset/offset (%) appeared earlier and later, respectively, with an increase in running speed. The absolute duration of RF activation (s) was elongated with the decrease in absolute running cycle time (increase in running speed). Both Switch and Scissors showed significant negative correlations with running speed and step frequency. CONCLUSIONS: The RF and BF excitation in both legs, as evidenced by changes in both Switch and Scissors, is coordinated for controlling running speed, as well as step frequency.

Biomechanical analysis of distance adjustment in volleyball overhead pass.

In volleyball, the overhead pass is important. Coaches usually teach players to push the ball, utilising the legs, especially for a long distance pass. The purpose of this study was to conduct a biomechanical analysis of an overhead pass to different distances. Twelve male elite volleyball players participated. They were told to pass a ball with an overhead pass through a 2.43 m high ring located 3 m, 6 m, and 9 m in front of them. Twenty-four reflective markers were placed on the ball and the subject's body to obtain joint centres, joint angles, and ball position. EMG activities were recorded from four right arm muscles. Triceps brachii and flexor carpi radialis pull and push phase activities differed significantly between 3 m and 6 m, but not between 6 m and 9 m. On the other hand, leg movements were significantly different among 3 m, 6 m, and 9 m, suggesting that leg movements supplement the power given to the ball. Thus, as the distance to a target lengthens in an overhead pass, not only arm movement but also leg movement is required.

Activation of human spinal locomotor circuitry using transvertebral magnetic stimulation.

Transvertebral magnetic stimulation (TVMS) of the human lumbar spinal cord can evoke bilateral rhythmic leg movements, as in walking, supposedly through the activation of spinal locomotor neural circuitry. However, an appropriate stimulus intensity that can effectively drive the human spinal locomotor circuitry to evoke walking-like movements has not been determined. To address this issue, TVMS was delivered over an intervertebral space of the lumbar cord (L1-L3) at different stimulus intensities (10-70% of maximum stimulator output) in healthy human adults. In a stimulus intensity-dependent manner, TVMS evoked two major patterns of rhythmic leg movements in which the left-right movement cycles were coordinated with different phase relationships: hopping-like movements, in which both legs moved in the same direction in phase, and walking-like movements, in which both legs moved alternatively in anti-phase; uncategorized movements were also observed which could not be categorized as either movement type. Even at the same stimulation site, the stimulus-evoked rhythmic movements changed from hopping-like movements to walking-like movements as stimulus intensity was increased. Different leg muscle activation patterns were engaged in the induction of the hopping- and walking-like movements. The magnitude of the evoked hopping- and walking-like movements was positively correlated with stimulus intensity. The human spinal neural circuitry required a higher intensity of magnetic stimulation to produce walking-like leg movements than to produce hopping-like movements. These results suggest that TVMS activates distinct neural modules in the human spinal cord to generate hopping- and walking-like movements.

The Timing of Thigh Muscle Activity Is a Factor Limiting Performance in the Deceleration Phase of the 100-m Dash.

PURPOSE: We aimed to examine the timing of electromyography activity of the rectus femoris (RF) and biceps femoris (BF) in both legs, as well as spatiotemporal variables (running speed (RS), step frequency (SF), step length (SL)) between the maximal speed (Max) phase (50-70 m) and the deceleration (Dec) phase (80-100 m) of the 100-m dash. METHODS: Nine track and field athletes performed the 100-m dash with maximal effort. Spatiotemporal variables of each 10-m section were measured. A portable wireless data logger was attached to the subject's lower back to record electromyographies. We calculated onset/offset timing (%) of RF and BF in both legs using a Teager-Kaiser Energy Operator filter (e.g., ipsilateral leg RF onset is "iRF-onset," contralateral leg BF onset is "cBF-onset") in a running cycle. RESULTS: The decreased RS in the Dec phase (P < 0.001) was due to a decreased SF (P < 0.001). Moreover, iRF-onset (P = 0.002), iRF-offset (P = 0.008), iBF-offset (P = 0.049), and cBF-offset (P = 0.017) in the Dec phase lagged in the running cycle as compared with the Max phase. Furthermore, the time difference between the swing leg RF activity (iRF-onset) and the contact leg BF activity (cBF-onset; "Scissors1") became bigger in the Dec phase (P = 0.041). Significant negative correlations were found between ΔiRF-onset and ΔSF (P = 0.045), and between ΔiBF-offset and ΔSF (P = 0.036). CONCLUSIONS: The decreased RS and SF in the Dec phase of the 100-m dash would be the delayed timing of the RF and BF activities in the same leg as well as the disturbed interleg muscular coordination.

Difference in gaze control ability between low and high skill players of a real-time strategy game in esports.

This research investigated the difference in aspects of gaze control between esports experts (Expert) and players with lower skills (Low Skill) while playing the real-time strategy game called StarCraft. Three versions of this game at different difficulty levels were made with the StarCraft Editor, and the gaze movements of seven Expert and nine Low Skill players were analyzed while they played the games. The gaze of Expert players covered a significantly larger area in the horizontal direction than the gaze of Low Skill players. Furthermore, the magnitude and number of saccadic eye movements were greater, and saccade velocity was faster in the Expert than in the Low Skill players. In conclusion, StarCraft experts have a specific gaze control ability that enables them to quickly and widely take visual information from all over the monitor. This could be one of the factors enabling StarCraft experts to perform better than players with lower skills when playing games that require task-switching ability.

Dynamic Control of Upper Limb Stretch Reflex in Wrestlers.

PURPOSE: The objective of this study was to clarify the characteristics of the upper limb stretch reflex in wrestlers. METHODS: Ten wrestlers and 11 control subjects participated in the study. The experiment was divided into two sessions. In the extension perturbation session, participants either relaxed or flexed the elbow when they felt a perturbation (abrupt elbow extension induced by a dynamometer). This was done 30 times by each subject for both sessions. In the flexion perturbation session, participants also relaxed or extended the elbow when they felt a perturbation (abrupt elbow flexion). During the tasks, the stretch reflex was monitored by recording the surface EMG activities of the right biceps and triceps brachii. The EMG reflex components were divided into three periods based on the time after the perturbation (M1, 20-50 ms; M2, 50-80 ms; and M3, 80-100 ms). The averaged background EMG activity just before the disturbance was subtracted from the EMG activity in each period. The resultant value was integrated to obtain reflex magnitudes of M1 to M3. RESULTS: For the triceps brachii, in the relaxation task, the wrestler group showed a significantly smaller value for M2 than did the control group. In the extension task, the wrestler group showed a significantly larger value for M3 than did the control group. There was no difference in M1 between the two groups. For the biceps brachii, there was no significant difference between any reflex components. CONCLUSIONS: Our results suggest that high-level wrestlers have specific characteristics of the long-latency stretch reflex in the triceps brachii that are modulated in a situation-specific manner.

Spatiotemporal inflection points in human running: Effects of training level and athletic modality.

The effect of the different training regimes and histories on the spatiotemporal characteristics of human running was evaluated in four groups of subjects who had different histories of engagement in running-specific training; sprinters, distance runners, active athletes, and sedentary individuals. Subjects ran at a variety of velocities, ranging from slowest to fastest, over 30 trials in a random order. Group averages of maximal running velocities, ranked from fastest to slowest, were: sprinters, distance runners, active athletes, and sedentary individuals. The velocity-cadence-step length (V-C-S) relationship, made by plotting step length against cadence at each velocity tested, was analyzed with the segmented regression method, utilizing two regression lines. In all subject groups, there was a critical velocity, defined as the inflection point, in the relationship. In the velocity ranges below and above the inflection point (slower and faster velocity ranges), velocity was modulated primarily by altering step length and by altering cadence, respectively. This pattern was commonly observed in all four groups, not only in sprinters and distance runners, as has already been reported, but also in active athletes and sedentary individuals. This pattern may reflect an energy saving strategy. When the data from all groups were combined, there were significant correlations between maximal running velocity and both running velocity and step length at the inflection point. In spite of the wide variety of athletic experience of the subjects, as well as their maximum running velocities, the inflection point appeared at a similar cadence (3.0 ± 0.2 steps/s) and at a similar relative velocity (65-70%Vmax). These results imply that the influence of running-specific training on the inflection point is minimal.

Difference in racket head trajectory and muscle activity between the standard volley and the drop volley in tennis.

Among tennis coaches and players, the standard volley and drop volley are considered basically similar, but muscles need to be relaxed (deactivation) just at the moment of impact when hitting the drop volley. However, this is not evidence-based. The aim of this study was to clarify racket head trajectory and muscle activity during the drop volley and to compare them with those of the standard volley. We hypothesized that 1) the racket head would move less forward for the drop volley than for the standard volley and 2) the wrist and elbow muscles be relaxed for the drop volley at the time of ball impact. Eleven male college students with sufficient tennis experience volunteered to participate in this study. Wireless EMG sensors recorded activation of the four arm muscles. Each subject performed the standard volley or the drop volley with both a forehand and a backhand from a position near the net. Four high speed video cameras (300 Hz) were set up on the court to measure ball speed and racket head trajectory. Returned ball speed of the drop volley was significantly lower than that of the standard volley (p < 0.05). The racket head moved less forward than in the standard volley, supporting the first hypothesis. Muscle activity of the drop volley, just before and after ball impact for both the forehand and backhand, was lower than that of the standard volley. However, the activity was in the form of a gradual increase as impact time approached, rather than a sudden deactivation (relaxation), which did not support the second hypothesis. For the drop volley, lower muscle activity in the forearm enabled a softer grip and thus allowed a "flip" movement of the racket to diminish the speed of the returned ball.

Recognition capability of one's own skilled movement is dissociated from acquisition of motor skill memory.

When we have rehearsed a movement using an object, we can reproduce the movement without holding the object. However, the reproduced movement sometimes differs from the movement holding a real object, likely because movement recognition is inaccurate. In the present study, we tested whether the recognition capability was dissociated from the acquisition of motor skill memory. Twelve novices were asked to rotate two balls with their right hand as quickly as possible; they practiced the task for 29 days. To evaluate recognition capability, we calculated the difference in coordination pattern of all five digits between the ball-rotation movement and the reproduced movement without holding balls. The recognition capability did not change within the first day, but improved after one week of practice. On the other hand, performance of the ball rotation significantly improved within the first day. Since improvement of performance is likely associated with acquisition of motor skill memory, we suggest that recognition capability, which reflects the capability to cognitively access motor skill memory, was dissociated from the acquisition of motor skill memory. Therefore, recognition of one's own skilled movement would rely on a hierarchical structure of acquisition of motor skill memory and cognitive access to that memory.

Determinants of Neural Plastic Changes Induced by Motor Practice.

Short-term motor practice leads to plasticity in the primary motor cortex (M1). The purpose of this study is to investigate the factors that determine the increase in corticospinal tract (CST) excitability after motor practice, with special focus on two factors; "the level of muscle activity" and "the presence/absence of a goal of keeping the activity level constant." Fifteen healthy subjects performed four types of rapid thumb adduction in separate sessions. In the "comfortable task" (C) and "forceful task" (F), the subjects adducted their thumb using comfortable and strong forces. In the "comfortable with a goal task" (CG) and "forceful with a goal task" (FG), subjects controlled the muscle activity at the same level as in the C and F, respectively, by adjusting the peak electromyographic amplitude within the target ranges. Paired associative stimulation (PAS), which combines peripheral nerve (median nerve) stimulation and transcranial magnetic stimulation (TMS), with an inter-stimulus interval of 25 ms (PAS25) was also done. Before and after the motor tasks and PAS25, TMS was applied to the M1. None of the four tasks showed any temporary changes in behavior, meaning no learning occurred. Motor-evoked potential (MEP) amplitude increased only after the FG and it exhibited a positive correlation with the MEP increase after PAS25, suggesting that FG and PAS25 share at least similar plasticity mechanisms in the M1. Resting motor threshold (RMT) decreased only after FG, suggesting that FG would also be associated with the membrane depolarization of M1 neurons. These results suggest task-dependent plasticity from the synergistic effect of forceful muscle activity and of setting a goal of keeping the activity level constant.

Timing of Rectus Femoris and Biceps Femoris Muscle Activities in Both Legs at Maximal Running Speed.

PURPOSE: The purpose of this study was to investigate the relationship between spatiotemporal variables of running and onset/offset timing of rectus femoris (RF) and biceps femoris (BF) muscle activities in both legs. METHODS: Eighteen male well-trained athletes (age = 20.7 ± 1.8 yr) were asked to run 50 m at maximal speed. The spatiotemporal variables (running speed, step frequency, and step length) over the distance from 30 to 50 m were measured. In addition, RF and BF muscle activities were obtained from both legs using wireless EMG sensors. To quantify the onset and offset timing of muscle activity, the band-pass filtered (20-450 Hz) EMG signal was processed using a Teager-Kaiser energy operator filter. We calculated RF and BF onset/offset timings (%) in both legs (e.g., ipsilateral leg RF [iRF] and contralateral leg BF [cBF]) during running cycle. Based on those timings, we obtained the EMG timing variables (%) as follows: "Switch1 (iBF-offset to iRF-onset)," "Switch2 (iRF-offset to iBF-onset)," "Scissors1 (cBF-onset to iRF-onset)," and "Scissors2 (iRF-offset to cBF-offset). RESULTS: We found that "Switch2" had positive (r = 0.495, P = 0.037), "Scissors1" had negative (r = -0.469, P = 0.049), and "Scissors2" had positive (r = 0.574, P = 0.013) correlations with step frequency. However, these variables had no significant correlations with running speed or step length. CONCLUSIONS: These results indicate that higher step frequency would be achieved by smoother switching of the agonist-antagonist muscle activities and earlier iRF activation relative to the cBF activity. To improve sprint performance, athletes and coaches should consider not only muscle activities in one leg but also coordination of muscle activities in both legs.

Biomechanical analysis of volleyball overhead pass.

In volleyball, the overhead pass is an important skill for passing a ball and making a set to attackers. In the present study, we analysed the overhead pass motion and involved muscle activity with special attention to the stretch-shortening cycle (SSC). Electromyogram was recorded from the wrist and elbow joint flexors and extensors. The movement period when the ball touched the hand was divided into the pull and push phases with the moment when the ball was at the lowest position. In the pull phase, although the wrists were extended, the activity of wrist flexor muscle was higher than the extensor muscle in both skilled and unskilled groups. And the activity in the wrist flexor muscle in the pull phase was higher in the skilled participants than that of the unskilled participants. Thus, the skilled participants pushed the ball by utilising the SSC of the wrist, while the unskilled participants did this in a less degree. In addition, the kinetic chain was observed from elbow to wrist in the skilled participants. We conclude that it is important for an overhead pass that the wrist flexor muscles accumulate elastic energy during the pull phase which is then released in the push phase.

Stability of bimanual finger tapping coordination is constrained by salient phases.

In bimanual cyclical continuous movements, the relative timing of the most salient movement phase in each movement is a predominant constraint. This is the case for coordination when both movements have a single most salient phase (the relative-salience hypothesis). We tested whether the relative-salience hypothesis could explain results obtained for repetitive discrete movements, utilizing finger tapping. In experiment 1, participants performed unimanual alternate two-finger tapping with the metronome beat (i.e., one finger taps on the beat and the other finger taps off the beat). The stability of the tapping timing relative to the beat, which reflects the extent of salience, was higher in the index finger than the middle finger, and was lower in the ring finger than the middle finger. In experiment 2, participants performed four conditions of repetitive bimanual four-finger tapping (i.e., alternate two-finger tapping in each hand) without external pacing signals. Under all four conditions, a more stable pattern occurred when the timing of the more salient tapping in each hand was simultaneous rather than alternate, regardless of relative direction in the external space or movement coupling of the homologous fingers. The results indicated that bimanual four-finger tapping could be explained by the relative-salience hypothesis.

Martial arts training is related to implicit intermanual transfer of visuomotor adaptation.

Recent work identified an explicit and implicit transfer of sensorimotor adaptation with one limb to the other, untrained limb. Here, we pursue the idea that different individual factors contribute differently to the amount of explicit and implicit intermanual transfer. In particular, we tested a group of judo athletes who show enhanced right-hemispheric involvement in motor control and a group of equally trained athletes. After adaptation to a 60° visual rotation, we estimated awareness of the perturbation and transfer to the untrained, non-dominant left hand in two experiments. We measured the total amount of intermanual transfer (explicit plus implicit) by telling the participants to repeat what was learned during adaptation, and the amount of implicit transfer by instructing the participants to refrain from using what was learned and to perform movements as during baseline instead. We found no difference between the total intermanual transfer of judokas and running experts, with mean absolute transfer values of 42.4° and 47.0°. Implicit intermanual transfer was very limited, but larger in judokas than in general sports athletes, with mean values of 5.2° and 1.6°. A multiple linear regression analysis further revealed that total intermanual transfer, which mainly represents the explicit transfer, is related to awareness of the perturbation, while implicit intermanual transfer can be predicted by judo training, amount of total training, speed of adaptation, and handedness scores. The findings suggest that neuronal mechanisms such as hemispheric interactions and functional specialization underlying intermanual transfer of motor learning may be applied according to individual predisposition.

Comparison of the Position-Matching and Position-Reproducing Tasks to Detect Deficits in Knee Position Sense After Reconstruction of the Anterior Cruciate Ligament.

CONTEXT: Deficits in knee position sense following reconstruction of the anterior cruciate ligament (ACL) can delay an athlete's return to sport participation and increase the risk of reinjury. Deficits in position sense postreconstruction have been evaluated using either a position-reproducing or position-matching task. OBJECTIVE: The aim of our study was to combine both to determine which assessment would be more effective to identify deficits in knee position sense. DESIGN: Longitudinal laboratory-based study. PARTICIPANTS: Eleven athletes (6 men and 5 women; mean age, 20.5 [1.2] y), who had undergone ACL reconstruction with an ipsilateral hamstring autograft, and 12 age-matched controls. INTERVENTIONS: Position sense was evaluated at 6 and 12 months postreconstruction and once for the control group. In addition, peak isokinetic knee extension and flexion strength, at 60°/s and 180°/s, was assessed for the ACL reconstruction group to evaluate possible influences of muscle strength on knee joint position sense. MAIN OUTCOME MEASURES: The variables include the angular differences between the reference limb and indicator limb, and peak torque values of isokinetic knee extension and flexion. RESULTS: Significant matching differences were identified at 6 months postsurgery on the position-matching task, but not at 12 months postsurgery. No significant between-group and within-subject differences were identified on the position-reproducing task. No significant matching errors were identified for the control group. There was no correlation between errors in position sense and maximum isokinetic strength. CONCLUSION: The position-matching task is more sensitive than the position-reproducing task to identify deficits in knee position sense over the first year following ACL reconstruction surgery.

Brain Activity Underlying Muscle Relaxation.

Fine motor control of not only muscle contraction but also muscle relaxation is required for appropriate movements in both daily life and sports. Movement disorders such as Parkinson's disease and dystonia are often characterized by deficits of muscle relaxation. Neuroimaging and neurophysiological studies suggest that muscle relaxation is an active process requiring cortical activation, and not just the cessation of contraction. In this article, we review the neural mechanisms of muscle relaxation, primarily utilizing research involving transcranial magnetic stimulation (TMS). Several studies utilizing single-pulse TMS have demonstrated that, during the relaxation phase of a muscle, the excitability of the corticospinal tract controlling that particular muscle is more suppressed than in the resting condition. Other studies, utilizing paired-pulse TMS, have shown that the intracortical inhibition is activated just before muscle relaxation. Moreover, muscle relaxation of one body part suppresses cortical activities controlling other body parts in different limbs. Therefore, the cortical activity might not only be a trigger for muscle relaxation of the target muscles but could also bring about an inhibitory effect on other muscles. This spread of inhibition can hinder the appropriate contraction of muscles involved in multi-limb movements such as those used in sports and the play of musical instruments. This may also be the reason why muscle relaxation is so difficult for beginners, infants, elderly, and the cognitively impaired.

Walking and finger tapping can be done with independent rhythms.

Rhythmic movements occur in many aspects of daily life. Examples include clapping the hands and walking. The production of two independent rhythms with multiple limbs is considered to be extremely difficult. In the present study we evaluated whether two different, independent rhythms that involved finger tapping and walking could be produced. In Experiment I, twenty subjects that had no experience of musical instrument training performed rhythmic finger tapping with the right index finger and one of four different lower limb movements; (1) self-paced walking, (2) given-paced walking, (3) alternative bilateral heel tapping from a sitting position, and (4) unilateral heel tapping with the leg ipsilateral to the tapping finger from a sitting position. The target intervals of finger tapping and heel strikes for walking step/heel tapping were set at 375 ms and 600 ms, respectively. The even distribution of relative phases between instantaneous finger tapping and heel strike was taken as the criteria of independency for the two rhythms. In the self-paced walking and given-paced walking tasks, 16 out of 20 subjects successfully performed finger tapping and walking with independent rhythms without any special practice. On the other hand, in the bipedal heels striking and unipedal heel striking tasks 19 subjects failed to perform the two movements independently, falling into interrelated rhythms with the ratio mostly being 2:1. In Experiment II, a similar independency of finger tapping and walking at a given pace was observed for heel strike intervals of 400, 600, and 800 ms, as well as at the constant 375 ms for finger tapping. These results suggest that finger tapping and walking are controlled by separate neural control mechanisms, presumably with a supra-spinal locus for finger tapping, and a spinal location for walking.