Salivary Aldosterone, Cortisol, and Their Morning to Evening Slopes in Patients with Depressive Disorder and Healthy Subjects: Acute Episode and Follow-Up 6 Months after Reaching Remission.

BACKGROUND/OBJECTIVE: Cortisol is thought to be involved in the pathophysiology of affective disorders. Less attention has been given to other neuroendocrine factors. The aim of the present study was to test the hypothesis that adrenocortical steroids aldosterone and cortisol show different dynamic changes in the course of clinical depression with the assumption that aldosterone is a state marker of depression. METHODS: A total of 78 adult subjects (39 patients with depressive disorder and 39 healthy controls) participated in a prospective non-interventional clinical study. Patients were investigated at the time of an acute episode and 6 months after reaching remission. The clinical and personal characteristics, and morning and evening salivary concentrations of aldosterone and cortisol were evaluated. RESULTS: Patients with an acute depressive episode exhibited higher evening aldosterone and lower morning cortisol concentrations compared to healthy subjects. In these patients, both hormone concentrations showed flatter morning to evening slopes. Salivary aldosterone, but not cortisol concentrations, were lower in patients 6 months after reaching remission compared to those in the acute state. Similarly, 6 months of remission resulted in a steeper morning to evening slope of salivary aldosterone compared to the acute state. The cortisol rhythm remained dysregulated. A significant negative correlation between trait anxiety scores and morning cortisol concentrations in patients at 6 months of clinical remission was observed. CONCLUSION: Diurnal changes in salivary aldosterone concentrations appear to be a state marker, whilst those of cortisol a trait marker of depression.

Higher perceived stress is associated with lower cortisol concentrations but higher salivary interleukin-1beta in socially evaluated cold pressor test.

The relationship between subjective stress perception and the objective stress response to acute stress stimuli is not sufficiently understood. The aim of the present study was to test the hypothesis that the neuroendocrine response in socially evaluated cold pressor test (CPT) depends on the extent of perceived stressfulness of the stimulus. The test was performed in 24 healthy male volunteers. Subjective stress perception was assessed using nine visual analog scales. The subjects were divided to low and high stress perception groups according to the median split of the scores. Subjects with high stress perception exhibited slightly lower values of systolic blood pressure and lower overall concentrations of salivary cortisol compared to subjects with low stress perception. Salivary alpha-amylase activity did not show significant changes. Salivary aldosterone decreased in time in subjects with low but increased early after the test in subjects with high stress perception. Interestingly, salivary concentrations of the pro-inflammatory cytokine interleukin-1beta were considerably higher in subjects with high stress perception, particularly immediately before the test. The differences in salivary cortisol and interleukin-1beta were confirmed by the analysis with distress as a continuous covariate. Distress scores correlated negatively with salivary cortisol and positively with interleukin-1beta. The rate pressure product, which is a global measure of energy consumption by the heart, was significantly higher immediately before than after the stress exposure. The present findings show that concentrations of interleukin-1beta are a sensitive component of the stress response at the time before the stressful event.Lay summaryIt is generally expected that higher perceived stressfulness of a stimulus is accompanied by higher activation of stress-related systems. This study evaluating a combined psychosocial and physical stress situation in healthy men provides evidence that individual parameters of the stress response are differently related to perceived stress intensity. Subjects with high stress perception exhibited lower systolic blood pressure and salivary cortisol, higher interleukin-1beta, marginal differences in alpha amylase and aldosterone compared to subjects with low stress perception, which might be important for stress coping.

The impact of selected environmental, behavioral and psychosocial factors on schoolchildren's somatic and mental health.

Children develop rapidly and many exogenous determinants of health significantly affect their somatic and mental development. There is a subjective perception of cognitive load associated with the educational process. The aim of the study is to assess individual environmental, behavioral and psychosocial factors influencing physical health and to investigate the amount of mental load in children. We investigated 87 schoolchildren (47 girls and 40 boys) aged 10-12 years, who were attending primary school in Bratislava. To assess values of selected factors we used a questionnaire form and personality characteristics were estimated by standardized psycho-diagnostic and IQ tests [range of classic fear, social-situation anxiety and jitters [skala Klasickeho strachu a Socialno-situacnej (in Slovak)] (KSAT), Eyesenck Personality Questionnaire for children (EPQ), Raven's IQ test]. Self-reported perception of mental load was assessed by questionnaire of subjective feelings and states (SFS). Children's body parameters were assessed using anthropometric measurements [height, weight, chest, abdominal and hip girth, Rohrer's index (RI), body mass index (BMI)] and a body fat measurement method (skinfold thickness). The results confirmed a significant relationship between higher parameters of overweight and obesity and irregular breakfast eating (p<0.05), absence of family dining (p<0.05), exposure to tobacco smoke in the family environment (p<0.01) and mother's level of education (p<0.05). Almost 60% of children reported high mental effort and cognitive load associated with the educational process. We note a close relationship between the higher mental load and the score of neuroticism (p<0.01). The physical and mental health of schoolchildren is significantly affected by exogenous factors. Therefore, in terms of protection and promotion of children's health, it is important to evaluate and monitor environmental risk factors and to form their healthy habits.