Comparison of the functions of plasma membrane and vacuolar membrane lipids in plant cell protection against hyperosmotic stress.

MAIN CONCLUSION: The comparison of the changes of the lipid content in plant cell boundary membranes demonstrates a substantial role of the vacuolar membrane in response to hyperosmotic stress. Comparison of variations in the lipid content of plant cell boundary membranes (vacuolar and plasma membranes) isolated from beet root tissues (Beta vulgaris L.) was conducted after the effect of hyperosmotic stress. Both types of membranes participate in the formation of protective mechanisms, but the role of the vacuolar membrane was considered as more essential. This conclusion was connected with more significant adaptive variations in the content and composition of sterols and fatty acids in the vacuolar membrane (although some of the adaptive variations, especially, in the composition of phospholipids and glycoglycerolipids were similar for both types of membranes). In the plasma membrane under hyperosmotic stress, the increase in the content of sphingolipids was noted that was not observed in the tonoplast.

Role of tonoplast microdomains in plant cell protection against osmotic stress.

MAIN CONCLUSION: Variations in the content of tonoplast microdomains, isolated with the aid of a non-detergent technique, are induced by osmotic stress and may take part in plant cell adaptive mechanisms. Investigation of tonoplast microdomain lipids isolated with the aid of the non-detergent technique from beetroots (Beta vulgaris L.) subjected to either hyperosmotic or hypoosmotic stress was conducted. Earlier, an important role of tonoplast lipids in the protection of plant cells from stress was demonstrated (Ozolina et al. 2020a). In the present paper, we have put forward a hypothesis that lipids of microdomains of raft nature present in the tonoplast are responsible for this protective function. The variations in the content of lipids of the studied nondetergent-isolated microdomains (NIMs) under hyperosmotic and hypoosmotic stresses were different. Under hyperosmotic stress, in the scrutinized microdomains, some variations in the content of lipids were registered, which were characteristic of the already known protective anti-stress mechanisms. These variations were represented by an increase in sterols and polar lipids capable of stabilizing the bilayer structure of the membranes. The found variations in the content of sterols may be bound up with some intensification of the autophagy process under stress because sterols foster the formation of new membrane contacts necessary for this process. Under hypoosmotic stress, the pattern of redistribution of the lipids in the scrutinized membrane structures was different: the largest part of the lipids appeared to be represented by hydrocarbons, which fulfilled mainly a protective function in plants and could prevent the excess water influx into the vacuole. The results obtained not only demonstrate the possible functions of the vacuolar membrane microdomains but also put forward an assumption on the role of any membrane microdomain in the protection mechanisms of the plant cell.