Excessive zinc intake increases systemic blood pressure and reduces renal blood flow via kidney angiotensin II in rats.

This study investigated the effects of excess zinc intake on the mean arterial pressure (MAP), renal blood flow (RBF), inulin clearance (IC), serum zinc level, serum angiotensin-converting enzyme (ACE) activity, and kidney angiotensin II (AT II) levels in rats. Experiments were performed on male Sprague-Dawley rats maintained for 4 weeks on a diet containing either 5 mg/100 g (control group), 50 mg/100 g (Zn50 group), or 200 mg/100 g (Zn200 group) zinc carbonate. Serum zinc levels significantly increased to 126.5 % in the Zn50 group and 198.1 % in the Zn200 group compared with controls. MAP significantly increased to 107.8 % in the Zn50 group and 114.5 % in the Zn200 group again compared with controls. Although the difference in serum ACE activity was independent of the serum zinc levels, the kidney AT II levels increased significantly to 137.2 % in the Zn50 group and 174.4 % in the Zn200 group compared with the controls. RBF was decreased significantly to 74.4 % in the Zn50 group and 69.7 % in the Zn200 group compared with the controls. IC values were significantly decreased to 69.6 % in the Zn50 group and 52.7 % in the Zn200 group as compared with control levels. Combined together, these results show that excessive Zn intake reduced IC and RBF and increased MAP and kidney AT II levels, suggesting that excessive Zn intake reduces renal function.

[New parameters "average flow" and "acceleration wave": comparison with current parameters in the flow volume curve].

AIM: The flow volume curve is an essential test method for diagnosis and treatment of the respiratory diseases. However, this curve depends on patient's continuous effort toward optimal expiration and it has been reported that differences in this effort may possibly result in error in flow speed. To overcome the potential error, we devised the "average flow" and the "acceleration wave" that comprehends the overall data of the expiration flow speed, and have done comparative analysis with the current parameters. METHODS: The average flow is derived by taking the integration from the beginning of the expiration to the end, and divides the integrated value by the number of data counts. Additionally, the acceleration wave is derived by taking the second degree derivative of the flow volume curve. RESULTS: The average flow showed strong correlation among healthy male and healthy female V50, obstructive index and criteria for COPD severity patients. Also, we were able to obtain the maximum acceleration from the acceleration wave. Significantly, this value showed strong correlation with the COPD patient's peak flow and average flow/peak flow. CONCLUSIONS: If the ratio of the average flow and the peak flow is below a fixed criterion, it is an obstructive lung disease, if it is above, it is possible to detect restrictive lung disease. Since the maximum acceleration rate of the acceleration wave is derived by the start of the expiration nearly up to 100 ml, it is especially possible to detect minute changes of the flow speed in large respiratory tract.

[Pre-analytical problems on assay conditions for blood midkine].

We have established the assay conditions of midkine (MK) measurement, the reference intervals and evaluation for clinical significance of blood MK measurement. MK is a kind of cytokines and basic protein which is a heparin-binding growth factor of various cells. The increase of MK expression suggests a prognostic value in early stage on cancers or inflammation. But significant problems in the MK measurement are alterations resulting from standing time and temperature instability after blood collection. Assay of MK was performed with solid phase human MK immunoassay recently developed sensitive enzyme linked immunosorbent method. The assay condition of MK was required to be separated immediately after blood sampling within 24 hrs at 4 degrees C or within 2 hrs at room temperature-standing. Plasma sample obtained with EDTA-2Na or citric acid-Na, and serum obtained from plain tube container showed good results. Linearity was obtained up to 1500 pg/ml and repeatability and reproducibility were within 10% as CV%. The recovery of MK was 101.1+/-3.8% with 10 specimens ranged 97-105%. Addition of interfering substances showed no effect on assay results when hemoglobin, EDTA-Na, citrate and turbidity check, but conjugated bilirubin (over 0.68 mmol/l) and gave negative errors within 10% in the assay results and heparin gave negative errors. The reference interval was 550 +/- 160 pg/ml in healthy individuals serum.

Prolactin increases open-channel density of epithelial Na+ channel in adult frog skin.

The short-term effect of prolactin on the skin of the adult tree frog Hyla arborea japonica was investigated using current-fluctuation analysis. Basolateral application of ovine prolactin (10 microg ml(-1)) (1) increased the amiloride-blockable short-circuit current (SCC) across the skin 2.6+/-0.4-fold and (2) increased the open-channel density (M) of the epithelial Na(+) channel 6.1+/-1.2-fold but decreased the single-channel current i to 0.4+/-0.1 times the control value (N=9). The increase in SCC induced by prolactin was thus due to an increase in M, not i. Apparently, in amphibians prolactin has not only a counteracting effect on metamorphosis but also a stimulatory effect on the development of adult-type features, such as this amiloride-blockable SCC.

Growth hormone is a weaker candidate than prolactin for the hormone responsible for the development of a larval-type feature in cultured bullfrog skin.

Prolactin (PRL) has, for some years, been considered to be the 'juvenile hormone' in amphibians. Recently, growth hormone (GH) has been proposed as another candidate, because in the larval stages the expression of the mRNA GH is high but it is downregulated in the climax stages of metamorphosis or following treatment with thyroid hormone. In the present study, we investigated whether GH promotes the development of one particular larval-type feature of bullfrog tadpole skin in vitro. The amiloride-, acetylcholine- and ATP-stimulated short-circuit current (SCC) is a physiological marker of larval-type bullfrog skin. These types of ligand-stimulated SCC (1) developed when EDTA-treated tadpole skin was cultured with corticoids supplemented with PRL or GH and (2) were not significantly different between skin cultured with PRL and intact tadpole skin. However, the amiloride-induced SCC response in skin cultured with GH differed in its kinetics from that of the intact (control) tadpole. On this basis, PRL seems a better candidate than GH for the juvenile hormone, at least with regard to the development of amiloride-stimulated non-selective cation channels.