RESUMEN
The microSiM (µSiM) is a membrane-based culture platform for modeling the blood-brain barrier (BBB). Unlike conventional membrane-based platforms, the µSiM provides experimentalists with new capabilities, including live cell imaging, unhindered paracrine signaling between 'blood' and 'brain' chambers, and the ability to directly image immunofluorescence without the need for the extraction/remounting of membranes. Here we demonstrate the basic use of the platform to establish monoculture (endothelial cells) and co-culture (endothelial cells and pericytes) models of the BBB using ultrathin nanoporous silicon-nitride membranes. We demonstrate compatibility with both primary cell cultures and human induced pluripotent stem cell (hiPSC) cultures. We provide methods for qualitative analysis of BBB models via immunofluorescence staining and demonstrate the use of the µSiM for the quantitative assessment of barrier function in a small molecule permeability assay. The methods provided should enable users to establish their barrier models on the platform, advancing the use of tissue chip technology for studying human tissues.
Asunto(s)
Barrera Hematoencefálica , Células Madre Pluripotentes Inducidas , Humanos , Barrera Hematoencefálica/metabolismo , Células Endoteliales/metabolismo , Encéfalo , Transporte Biológico , Técnicas de CocultivoRESUMEN
Advanced in vitro tissue chip models can reduce and replace animal experimentation and may eventually support "on-chip" clinical trials. To realize this potential, however, tissue chip platforms must be both mass-produced and reconfigurable to allow for customized design. To address these unmet needs, an extension of the µSiM (microdevice featuring a silicon-nitride membrane) platform is introduced. The modular µSiM (m-µSiM) uses mass-produced components to enable rapid assembly and reconfiguration by laboratories without knowledge of microfabrication. The utility of the m-µSiM is demonstrated by establishing an hiPSC-derived blood-brain barrier (BBB) in bioengineering and nonengineering, brain barriers focused laboratories. In situ and sampling-based assays of small molecule diffusion are developed and validated as a measure of barrier function. BBB properties show excellent interlaboratory agreement and match expectations from literature, validating the m-µSiM as a platform for barrier models and demonstrating successful dissemination of components and protocols. The ability to quickly reconfigure the m-µSiM for coculture and immune cell transmigration studies through addition of accessories and/or quick exchange of components is then demonstrated. Because the development of modified components and accessories is easily achieved, custom designs of the m-µSiM shall be accessible to any laboratory desiring a barrier-style tissue chip platform.
Asunto(s)
Células Madre Pluripotentes Inducidas , Silicio , Animales , Transporte Biológico , Barrera Hematoencefálica , Técnicas de CocultivoRESUMEN
We describe the extended endothelial cell culture method (EECM) for the differentiation of human pluripotent stem cells (hPSCs) into brain microvascular endothelial cell (BMEC)-like cells. EECM-BMEC-like cells resemble primary human BMECs in morphology, molecular junctional architecture, and diffusion barrier characteristics. A mature immune phenotype with proper endothelial adhesion molecule expression makes this model distinct from any other hPSC-derived in vitro blood-brain barrier (BBB) model and suitable to study immune cell migration across the BBB in a disease relevant and personalized fashion. For complete details on the use and execution of this protocol, please refer to Lian et al. (2014), Nishihara et al. (2020a).
Asunto(s)
Encéfalo/irrigación sanguínea , Diferenciación Celular , Endotelio Vascular/citología , Microvasos/citología , Células Madre Pluripotentes/citología , Células Cultivadas , Endotelio Vascular/inmunología , Humanos , Microvasos/inmunología , Células Madre Pluripotentes/inmunologíaRESUMEN
BACKGROUND: Evaluation of the maxillary sinus anatomy prior to sinus lift procedures is important to avoid surgical complications due to the close anatomical relationship between the posterior maxillary region and the maxillary sinüs. INTRODUCTION: In order to avoid traumatizing the posterior superior alveolar artery and to prevent perioperative bleeding, locating the exact position of the artery is imperative before the surgical procedures. METHODS: 150 CBCT scans were evaluated. The distance from the inferior border of the PSAA to the alveolar crest (the vertical line from the artery to the crest) and floor of the maxillary sinus, the distance from PSAA to medial sinus wall, nasal septum, zygomatic arch, position, the distance from the floor of maxillary sinüs to the alveolar crest and diameter of the PSAA were assessed. Locations of the artery were classified. RESULTS: The artery diameters were mostly ≥ 1 mm. The artery was mostly intraosseous (59.7%), 21.7% was superficial and only 18.7% was intra-sinuscular. CONCLUSION: The location of PSAA is intraosseous in most patients. The artery diameters were mostly ≥ 1 mm and we can say that increasing the size also increases the risk of complications.