Anthropometric analysis of orbital and nasal parameters for sexual dimorphism: New anatomical evidences in the field of personal identification through a retrospective observational study.

Orbital and nasal parameters among modern humans show considerable variation, which affects facial shape, and these characteristics vary according to race, region, and period in evolution. The aim of the study was to ascertain whether there are sex differences in the orbital and/or nasal indexes and/or the single measurements used to calculate these in a Kosovar population. The following parameters were taken into consideration: orbital height (OH), orbital width (OW), nasal height (NH), and nasal width (NW). The ratios between orbital index/nasal index (RONI) were calculated. All measurements were obtained from a population sample comprising 408 individuals. The accuracy in sex prediction was 52.86% (CI95% = 45.05%-60.67%) for NW and 64.96% for NH (CI95% = 57.50%- 72.42%). The difference between male and female indexes was statistically significant (P < 0.05). The anthropometric study revealed that only NW and NH are configured as predictors of sexual dimorphism. It could be useful to increase the number of samples to test the discriminant function in other population groups.

Third molar maturity index (I3M) assessment according to different geographical zones: a large multi-ethnic study sample.

Identification of living undocumented individuals highlights the need for accurate, precise, and reproducible age estimation methods, especially in those cases involving minors. However, when their country of origin is unknown, or it can be only roughly estimated, it is extremely difficult to apply assessment policies, procedures, and practices that are accurate and child-sensitive. The main aim of this research is to optimize the correct classification of adults and minors by establishing new cut-off values for four different continents (Africa, America, Asia, and Europe). For this purpose, a vast sample of 10,701 orthopantomographs (OPTs) from four continents was evaluated. For determination and subsequent validation of the new third molar maturity index (I3M) cut-off values by world regions, a cross-validation by holdout method was used and contingency tables (confusion matrices) were generated. The lower third molar maturity indexes, from both left and right side (I3ML and I3MR) and the combination of both sides (I3ML_I3MR) were calculated. The new cut-off values, that aim to differentiate between a minor and an adult, with more than 74.00% accuracy for all populations were as follows (I3ML; I3MR; I3ML_I3MR, respectively): Africa = (0.10; 0.10; 0.10), America = (0.10; 0.09; 0.09), Asia = (0.15; 0.17; 0.14), and Europe = (0.09; 0.09; 0.09). The higher sensitivity (Se) was detected for the I3ML for male African people (91%) and the higher specificity (Sp) of all the parameters (I3ML; I3MR; I3ML_I3MR) for Europeans both male and female (> 91%). The original cut-off value (0.08) is still useful, especially in discriminating individuals younger than 18 years old which is the goal of the forensic methods used for justice.

Association between metabolic control and oral health in children with type 1 diabetes mellitus.

BACKGROUND: Type 1 diabetes mellitus is the most common chronic disease and can be reflected in the oral cavity. The aim of this study was to analyze the impact of metabolic control on saliva, dental caries, dental plaque, gingival inflammation, and cariogenic bacteria in saliva. METHODS: A case-control epidemiologic study was performed on children with type 1 diabetes (ages 10-15 years) separated into two groups: 34 children with good metabolic control of glycated hemoglobin (HbA1c < 7.5%) and 46 children with poor metabolic control of glycated hemoglobin (HbA1c > 7.5%). Oral status was assessed using the Decay, Missing, and Filled Teeth index for permanent teeth (DMFT), plaque index and gingival index. The stimulated salivary flow rate was measured, and the colonies of Streptococcus mutans and Lactobacillus in saliva were determined. The observed children answered questions related to their frequency of brushing habits, dental visits and parents' education. Mann-Whitney U Test, Chi-Square test and Fisher's exact test were used in the statistical analyses. The significance level was set at p < 0.05. RESULTS: The children with diabetes with poor metabolic control had significantly higher values of the DMFT index, plaque index, and gingival index, more colonies, and a higher risk of Streptococcus mutans and Lactobacillus than the children with diabetes with good metabolic control (p < 0.001). The level of metabolic control of diabetes had no influence on salivary flow rates (p > 0.05). The majority of both groups with good and poor metabolic control brushed their teeth once per day, and they visited the dentist only when necessary (61.3%). However, the parents of children in both groups had medium to low levels of education, without any significant difference between groups (p > 0.05). CONCLUSION: The results indicate that children with diabetes have a lower level of oral hygiene and are potentially to dental caries and periodontal diseases, mainly when their metabolic disorder is uncontrolled.

Dental Caries Prevalence and Oral Health Status among 15-Year- Old Adolescents in Kosovo.

Introduction: Caries has a harmful impact on oral and general health and is a major public health problem among children and adolescents. The objective of present study was to investigate into dental caries, oral hygiene, the frequency of brushing habits and dental visits among 15- year- old adolescents. Material and methods: This cross-sectional study was carried out on a random sample of 323 15- year- old adolescents in different schools and municipalities of Kosovo. Oral clinical examination and self-administered questionnaire were used to obtain information about dental caries and oral health practices. Oral hygiene and caries status in permanent dentition was assessed through the DMFT index and Oral Hygiene Index - Simplified (OHI-S). The level of statistical significance was set at p<0.05. Results: The total mean of the DMFT index was 3.21 ± 2.193, while component D of the DMFT index dominated in both genders, with slightly higher values in boys compared to girls (2.15±2.092, and 1.91±1.919). The mean OHI-S index of adolescents aged 15 was found to be 1.945±3.926. Over 50% of schoolchildren brush their teeth only once per day and they have visited the dentist only when it was necessary. Irregular tooth-brushing, dental visits and poor oral hygiene index were significantly related to dental caries. Conclusion: The results of the study showed poor oral health status among 15- year- old adolescents in Kosovo. There is an emergent need for caries-prevention programs focusing on oral health and healthy habits.

Comparison of Frontal Sinuses for Personal Identification in 3 Populations Using Cameriere's Code Number.

ABSTRACT: The identification of unknown individuals is of crucial importance to society, especially in the context of mass disasters involving individuals of different nationalities. The comparison of frontal sinus patterns is an important tool for personal identification. In Cameriere's method, a new personal code number (codC) containing 8 digits was elaborated, based on the measurements of frontal sinus patterns. The present study is an update of this previous work. Digital radiographic images of the skulls of 299 individuals of different nationalities (100 Italians, 99 Kosovars, and 100 Turks) were analyzed, adopting the same maximum-weight dependence tree used in the original work. For comparisons between groups, Fisher exact test and the χ2 test were carried out. The method, applied to different population groups, significantly increases the likelihood of identifying a person by comparison of antemortem and postmortem frontal sinus radiographs. Results demonstrated that the model is more discriminative in identifying individuals of different nationalities.

Validity and reliability of the Psychosocial Impact of Dental Aesthetics Questionnaire in Kosovar adolescents.

OBJECTIVE: To test the validity and reliability of the Psychosocial Impact of Dental Aesthetics Questionnaire (PIDAQ)-Albanian version in Kosovar adolescents and assess gender-based differences of malocclusion on PIDAQ subscales. METHODS: A sample of 200 adolescents aged 12-15 years were evaluated for orthodontic treatment need by a previous calibrated dental examiner, using the aesthetic component (AC) of the Index of Orthodontic Treatment Need-Aesthetic Concern (IOTN-AC) and the dental aesthetic index (DAI). Internal consistency, test-retest reliability, discriminant validity and gender-based impact of malocclusion on psychosocial impact was assessed. RESULTS: The Albanian version of the PIDAQ shows satisfactory internal consistency (α ranging from 0.73 to 0.90) with high test-retest reliability (r > 0.83). The discriminant validity revealed that subjects with minor and severe malocclusion according to dichotomous DAI categorization had different PIDAQ scores. No significant gender-based differences were noted on the impact of malocclusion on psychological and social aspect or Dental Self-confidence (DSC); however, significant gender-based difference was noted on the impact of malocclusion on AC. CONCLUSION: The Albanian version of the PIDAQ demonstrated good psychometric properties and applicability to this age group in Kosova.

Oral Health Status Among 12-Year-Old Schoolchildren in Kosovo

Abstract Objective: To evaluate the oral health status of 12-year-old schoolchildren in Kosovo. Material and Methods: The study involved 1204 schoolchildren aged 12 years from urban and rural areas, from different cities of Kosovo. The questionnaire included demographic data, gender, residence, dental status, oral hygiene, and daily brushing habits. The feasibility of the questionnaire was verified replicating it on 10% of the sample. Daily brushing habits were reported to frequency: as once per day, twice a day and rarely. Caries status was recorded in permanent dentition as DMFT and Oral Hygiene Index - Simplified (OHI-S) was used to assess oral hygiene status. The analysis included occurrences and means. The differences among means were tested using the student t-test (p<0.05). Results: The highest mean and standard deviation of DMFT and OHI-S index was found among rural schoolboys 3.67 ± 1.98 and OHI-S 1.75. In total sample, 54.1% of them brush their teeth only once a day, 39.7 % brush their teeth twice a day and only 6.2 % rarely brush their teeth. Conclusion: Preventive measures are needed to improve dental health in 12 years old schoolchildren.

Comparison of Shear Bond Strength Orthodontic Brackets Bonded to Zirconia and Lithium Disilicate Crowns.

AIM: of this study was to analyze Shear Bond Strength (SBS) and Adhesive Remnant Index (ARI) of ceramic and metallic orthodontic brackets bonded to zirconia or lithium-disilicate ceramics used for prosthetic restorations, conditioned with hydrofluoric acid (HFA) or phosphoric acid (PhA), as well as to determine the Porcelain Fracture Index (PFI), in order to examine the condition of the ceramic surface after debonding. MATERIAL AND METHODS: The research was conducted on 96 prepared all-ceramic samples divided into 8 groups combined from the type of ceramic material, orthodontic brackets, and surface conditioning. SBS was tested with Universal Testing Machine, and the samples were analyzed using a Scanning Electron Microscope, to determine ARI and PFI. Statistical data were processed using ANOVA, with the level of significance α = 0.05. RESULTS: Lithium-disilicate showed better bond strength in almost all groups. However, no significant difference between the groups was noticed and none of the factors had a significant influence on the mean values of SBS (p>0.05). Nevertheless, ARI significantly depended on the type of bracket (p = 0.005), and PFI significantly depended on the type of etchant (p = 0.029). CONCLUSION: The use of HFA for surface etching of zirconia and lithium-disilicate, does not cause a significant increase in the SBS values as compared to etching with PhA and silane application. Furthermore, HFA can weaken the surface structure of the ceramic, and considering its toxicity, might not be the best suitable conditioner prior to orthodontic bonding to lithium disilicate, and in particular to zirconia, also taking into account its crystalline structure.

Dental age estimation using four Demirjian's, Chaillet's and Willems' methods in Kosovar children.

BACKGROUND: Tooth formation was recognized as useful body system to assess maturity and predict age. Tooth mineralization is much less affected by the endocrine and different nutritional status than bone mineralization, and teeth formation provides a more reliable indication of chronological age. Demirjian et al. in 1973 presented a scoring system and method for dental age estimation on a sample of French-Canadian children. Chaillet et al. and Willems et al. modified original Demirjian method. This study aimed to evaluate the accuracy of four Demirjian's, Chaillet and Willems methods for age estimation in the children of Kosovo. MATERIALS AND METHODS: The cross-sectional study was based on the evaluation of the sample of 1022 orthopantomograms (OPTs) of healthy Kosovar children, aged between 5 and 14 years. OPTs were taken from the Radiology unit of University Dentistry Clinical Center of Kosova, as part of random clinical treatment. We tested the accuracy of four methods based on seven mandibular teeth, Demirjian from 1973 (Dem73) and 1976 (Dem76), Chaillet from 2005 (Chaillet) and Willems from 2001 (Willems) and two Demirjian's methods based on different sets of four teeth (Dem76PM1 and Dem76IN2). RESULTS: For most tested methods, we found statistically significant differences between the chronological age (CA) and dental age (DA) (p < 0.05). In males, the most accurate method were those using four teeth, Dem76IN2 (0.03 years) following by Dem76PM1 (-0.05 years), following those using seven teeth, Willems (-0.14 years), Chaillet (-0.24 years) and Dem73 (0.43 years). In females, dental age was the most accurate for the Willems method (-0.24 years) following Chaillet (-0.35 years), Dem76 (0.43 years) and Dem73 (0.55 years), while Dem76PM1 and Dem76IN2 overestimated by 0.45 years and 0.46 years, respectively. The mean absolute difference between DA and CA were between 0.61 years for the Willems, to 0.78 years for the Dem73 in males, and 0.64 years for the Willems to 0.75 years for the Dem76IN2 in females. CONCLUSION: The Willems method was the most accurate for estimating a dental age if all seven mandibular teeth are available for analysis, and we found the similar accuracy of Dem76PM1 and Dem76IN2 methods. Therefore, we may encourage their use for age estimation on the Kosovar children.

Antimicrobial efficacy of photodynamic therapy and light-activated disinfection on contaminated zirconia implants: An in vitro study.

BACKGROUND: We aimed to evaluate the antimicrobial efficacy of photodynamic therapy (PDT) and light-activated disinfection (LAD) on zirconia dental implants contaminated with three bacterial species and investigate if the PDT and LAD cause implant surface alterations. METHODS: Seventy-two zirconia dental implants were contaminated with a bacterial suspension of Prevotella intermedia, Actinomyces actinomycetemcomitans, and Porphyromonas gingivalis. The implants were subsequently randomly divided into four groups (n = 12 dental implants/each) according to the decontamination protocol: Group 1 (PDT1) - PDT (660 nm, 100 mW) with toluidine blue; Group 2 (PDT2) - PDT (660 nm, 100 mW) with phenothiazine chloride dye; Group 3 (LAD) - light emitting diode (LED) with toluidine blue; and Group 4 (TB) - toluidine blue without the application of light. Implants in the positive control (PC) group were treated with a 0.2% chlorhexidine-based solution, and implants assigned to the negative control (NC) group did not undergo any treatment. Each implant was then placed in tubes containing phosphate buffered saline (PBS) and vortexed for 60 s to remove the remaining bacteria from the implant surface. After 10-fold serial dilutions, 30 µl of the suspension was plated on Brucella agar plates. After 72 h, the colony forming units (CFU) were counted. Distinctive colonies were confirmed with MALDI Biotyper. The implants were analyzed using scanning electron microscope (SEM) to evaluate the possible surface alterations due to PDT or LAD. RESULTS: All study groups had significant reductions in the number of CFUs compared with the NC (p < 0.05). PDT1, the PDT2, and the LAD groups had the largest bacterial reduction with respect to each bacterial species separately and the total bacterial count, and they were more efficient compared with the TB group (p < 0.05). SEM analysis did not reveal any alterations of the implant surface after the treatment procedures. CONCLUSION: Both PDT protocols and LAD showed high and equal effectiveness in decontamination of zirconia dental implants.

The third molar maturity index in indicating the legal adult age in Kosovar population.

The third molar tooth, known as the "wisdom tooth," is the only tooth that can be used to determine legal adult age. This study aimed to test the accuracy of Cameriere's third molar maturity index (I3M) in assessing the legal adult age of 18 years in the Kosovar population. Orthopantomographs (OPTs) of 1221 healthy living Kosovar individuals, aged between 12 and 23 years and with no congenital or developmental anomalies, were analyzed. Intra-class correlation coefficients were 0.858 (95% CI, 0.786 to 0.906) and 0.852 (95% CI, 0.779 to 0.903) for intra-rater and for inter-rater agreement, respectively. The sample was divided into training dataset (800 OPTs) and test dataset (421 OPTs). The training dataset was used to generate the logistic regression model, while the test dataset was used to study the performance of the model. I3M and gender as independent variables and adult age (≥ 18 years) or minor age (< 18 years) as the dependent variable were used for logistic regression analysis. The receiver operating curve (ROC) analysis was used to determine the specific cut-off value of I3M for predicting adult age. The results showed that only I3M statistically significantly contributed to discriminating adults and minors. ROC analysis showed that the cut-off value of I3M < 0.08 was the best in discriminating adults and minors. An analysis of the test dataset (421 OPTs) showed that as I3M decreased the age gradually increased. The performance of the cut-off value of I3M < 0.08, to discriminate between adults and minors, was analyzed by contingency tables for both sexes. In males, the accurate classification (Acc) was 0.968 (95% CI, 0.926 to 0.985), the sensitivity (Se) was 0.962 (95% CI, 0.925 to 0.978), and the specificity (Sp) was 0.976 (95% CI, 0.929 to 0.995). The Bayes post-test probability (Bayes PTP) was 0.975 (95% CI, 0.905 to 1.00). In females, Acc was 0.909 (95% CI, 0.870 to 0.917), Se and Sp were 0.826 (95% CI, 0.787 to 0.834) and 0.991 (95% CI, 0.953 to 1.00) respectively, while Bayes PTP was 0.989 (95% CI, 0.926 to 1.00). Our data support the usefulness of I3M < 0.08 to indicate legal adult age in a Kosovar population.

Shear Bond Strength of Orthodontic Brackets Bonded to Zirconium Crowns.

BACKGROUND: An increasing demand for esthetic restorations has resulted in an increased use of all-ceramic restorations, such as zirconium. However, one of the challenges the orthodontist must be willing to face is how to increase bond strength between the brackets and various ceramic restorations.Bond strength can beaffected bybracket type, by the material that bracketsaremade of, and their base surface design or retention mode. ​: Aim: of this study was to perform a comparative analysis of the shear bond strength (SBS) of metallic and ceramic orthodontic brackets bonded to all-zirconium ceramic surfaces used for prosthetic restorations, and also to evaluate the fracture mode of these two types of orthodontic brackets. MATERIAL AND METHODS: Twenty samples/semi-crowns of all-zirconium ceramic, on which orthodontic brackets were bonded, 10 metallic and 10 ceramic polycrystalline brackets, were prepared for this research. SBS has been testedby Universal Testing Machine, with a load applied using a knife edged rod moving at a fixed rate of 1 mm/min, until failure occurred. The force required to debond the brackets was recorded in Newton, then SBS was calculated to MPa. In addition, the samples were analyzed using a digital camera magnifier to determine Adhesive Remnant Index (ARI). Statistical data were processed using t-test, and the level of significance was set at α = 0.05. RESULTS: Higher shear bond strength values were observed in metallic brackets bonded to zirconium crowns compared tothoseof ceramic brackets, with a significant difference. During the test, two of the ceramic brackets were partially or totally damaged. CONCLUSION: Metallic brackets, compared to ceramic polycrystalline brackets, seemed tocreate stronger adhesion with all-zirconium surfaces due to their better retention mode. Also, ceramic brackets showed higher fragility during debonding.

Prevalence of Dental Caries in Kosovar Adult Population.

Objectives. The aim of this study was to assess the prevalence of dental caries in the Kosovar adult population. Materials and Methods. A cross-sectional study in Kosovo was conducted examining 9387 patients, aged 18 upwards, between January 2010 and December 2011. Clinical evaluation was done using WHO criteria for evaluation of dental health status and data collection. Results. The prevalence of caries for the whole study was 72.80%. The mean DMFT index was 9.61 (±5.12) in the 18-34-year age group, 11.6 (±6.42) in the 35-44-year age group, 13.68 (±8.12) among the 45-64-year age group, 17.98 (±9.81) in the 65-74-year age group, and 23.19 (±9.41) in the age group of 75+ years, respectively. A significant difference of mean DMFT and its each component was observed between the ages (P < 0.001). Conclusion. This study comes out with the significant levels of dental caries among young Kosovar population (18-34 years old).