Multigenerational toxicity of microplastics derived from two types of agricultural mulching films to Folsomia candida.

Degradation and fragmentation of mulching films represents an increasing source of microplastics (MPs, plastic particles 1 µm to 5 mm in size) to agricultural soils. MPs have been shown to affect many soil invertebrates, including springtails. However, these studies typically use test materials representing less environmentally relevant particle types, such as pristine uniform MPs, which do not represent the large range of particle sizes and morphologies found in the field. This study aimed at providing insight into the adverse effects of MPs originating from agricultural mulching films, by using artificially aged MPs derived from both biodegradable (starch-polybutadiene adipate terephthalate (PBAT)) blend, as well as conventional (linear low-density polyethylene (LLDPE)) plastic polymers. The soil dwelling springtail Folsomia candida was exposed to these MPs for five generations in order to elucidate population effects due to possible reproduction toxicity, endocrine disruption, mutagenesis or developmental toxicity. F. candida were exposed to 0, 0.0016, 0.008, 0.04, 0.2, 1, 2, 3, 4 and 5 % (w/w dry soil) MPs in Lufa 2.2 soil, which includes concentrations within the range of environmental relevance. Juveniles produced at each concentration were transferred to the next generation, with the parental, F2 and F4 generations being exposed for four weeks and F1 and F3 generations for five weeks. No concentration-dependent effects on F. candida survival or reproduction were observed in exposures to either of the MPs, in any of the generations. These results suggest that the particular MPs used in this study, derived from mulching films used on agricultural soils, may not be potent toxicants to F. candida, even after long-term exposure and at elevated concentrations.

Production and characterisation of environmentally relevant microplastic test materials derived from agricultural plastics.

Soil environments across the globe, particularly in agricultural settings, have now been shown to be contaminated with microplastics. Agricultural plastics - such as mulching films - are used in close or direct contact with soils and there is growing evidence demonstrating that they represent a potential source of microplastics. There is a demand to undertake fate and effects studies to understand the behaviour and potential long-term ecological risks of this contamination. Yet, there is a lack of test materials available for this purpose. This study describes the manufacture and characterisation of five large (1-40 kg) batches of microplastic test materials derived from agricultural mulching films. Batches were produced from either polyethylene-based conventional mulching films or starch-polybutadiene adipate terephthalate blend mulching films that are certified biodegradable in soil. Challenges encountered and overcome during the micronisation process provide valuable insights into the future of microplastic test material generation from these material types. This includes difficulties in micronising virgin polyethylene film materials. All five batches were subjected to a thorough physical and chemical characterisation - both of the original virgin films and the subsequent microplastic particles generated - including a screening for the presence of chemical additives. This is a critical step to provide essential information for interpreting particle fate or effects in scientific testing. Trade-offs between obtaining preferred particle typologies and time and cost constraints are elucidated. Several recommendations emerging from the experiences gained in this study are put forward to advance the research field towards greater harmonisation and utilisation of environmentally relevant test materials.

Multi-method analysis of microplastic distribution by flood frequency and local topography in Rhine floodplains.

Rivers are important transport pathways for microplastics into the ocean, but they can also be potential sinks due to microplastic deposition in the sediments of the river bed and adjacent floodplains. In particular, floods can (re)mobilise microplastics from sediments and floodplains, (re)deposit and relocate them depending on the floodplain topography. The knowledge about fluvial microplastic input to floodplains, their spatial distribution and their fate in floodplain soils is limited. To investigate this topic, we sampled soil at a depth of 5-20 cm along three transects in three different Rhine floodplains. We analysed the soil samples in tandem with pyrolysis GC/MS and ATR- & µ-FPA-FTIR for their microplastic abundance and mass concentrations. To study the influence of flood frequency on the microplastic abundance in the three floodplains, we fitted a hydrodynamic flood model (MIKE 21, DHI, Hørsholm, Denmark) and related the results to the respective spatial microplastic distribution. We found similar microplastic distribution patterns in each floodplain. The highest microplastic abundance (8516-70,124 microplastics kg-1) and mass concentration (46.2-141.6 mg kg-1) were consistently found in the farthest transects from the Rhine in a topographical depression. This microplastic distribution pattern is detectable with both, pyrolysis GC/MS and FTIR. The strongest correlation between the results of both methods was found for small, abundant microplastic particles. Our results suggest that the spatial distribution of microplastics in floodplains is related to the combination of flood frequency and local topography, that ought to be explicitly considered in future studies conducted in floodplains. Finally, our results indicate that pyrolysis GC/MS and FTIR data are comparable under certain conditions, which may help in the decision for the analytical method and sampling design in future studies.

Airborne microplastic concentrations and deposition across the Weser River catchment.

Microplastic (MP) appears to be omnipresent in the atmosphere, raising concerns about dispersion across environmental compartments, ecological consequences and human health risks by inhalation. To date, data on the sources of atmospheric MP and deposition to river catchment areas are still sparse. We, therefore, took aerosol and total atmospheric deposition samples in the catchment area of the large German river Weser to estimate microplastic deposition fluxes (DFs) at six specific sites and airborne MP concentrations. Sampling in rural, suburban, and urban environments and wastewater treatment plants (WWTPs) was performed, aiming at a variation in airborne MP pollution and elucidating potential MP source areas. Aerosol samples were taken twice in April and October while monthly total deposition samples were collected over a period from March to October. Microplastics were detected in all analysed aerosol samples by Raman spectroscopy down to 4 µm, and in all 32 total deposition samples by µFT-IR down to 11 µm. Average MP number concentrations of 91 ± 47 m-3 were found in aerosol samples. The measured total MP number DFs ranged between 10 and 367 N m-2 day-1 (99 ± 85 mean ± SD) corresponding to total deposition of 0.05 ± 0.1 kg ha-1 per year and to an estimated 232 metric tons of plastic being deposited in the Weser River catchment annually. MP number DFs were higher in urban than rural sites. An effect of WWTPs on the MP abundance in air was not observed. Polypropylene, polyethylene, polyethylene terephthalate, polyvinyl chloride, polystyrene, and silicone fragments were found as the predominant polymer types in total deposition samples, while polyethylene particles dominated in aerosol samples. The results suggest that proximity to sources, especially to cities, increase the numbers of MP found in the atmosphere. It further indicates that atmospheric MP considerably contributes to the contamination of both aquatic and terrestrial habitats.

Production of Organic Acids by Arbuscular Mycorrhizal Fungi and Their Contribution in the Mobilization of Phosphorus Bound to Iron Oxides.

Most plants living in tropical acid soils depend on the arbuscular mycorrhizal (AM) symbiosis for mobilizing low-accessible phosphorus (P), due to its strong bonding by iron (Fe) oxides. The roots release low-molecular-weight organic acids (LMWOAs) as a mechanism to increase soil P availability by ligand exchange or dissolution. However, little is known on the LMWOA production by AM fungi (AMF), since most studies conducted on AM plants do not discriminate on the LMWOA origin. This study aimed to determine whether AMF release significant amounts of LMWOAs to liberate P bound to Fe oxides, which is otherwise unavailable for the plant. Solanum lycopersicum L. plants mycorrhized with Rhizophagus irregularis were placed in a bicompartmental mesocosm, with P sources only accessible by AMF. Fingerprinting of LMWOAs in compartments containing free and goethite-bound orthophosphate (OP or GOE-OP) and phytic acid (PA or GOE-PA) was done. To assess P mobilization via AM symbiosis, P content, photosynthesis, and the degree of mycorrhization were determined in the plant; whereas, AM hyphae abundance was determined using lipid biomarkers. The results showing a higher shoot P content, along with a lower N:P ratio and a higher photosynthetic capacity, may be indicative of a higher photosynthetic P-use efficiency, when AM plants mobilized P from less-accessible sources. The presence of mono-, di-, and tricarboxylic LMWOAs in compartments containing OP or GOE-OP and phytic acid (PA or GOE-PA) points toward the occurrence of reductive dissolution and ligand exchange/dissolution reactions. Furthermore, hyphae grown in goethite loaded with OP and PA exhibited an increased content of unsaturated lipids, pointing to an increased membrane fluidity in order to maintain optimal hyphal functionality and facilitate the incorporation of P. Our results underpin the centrality of AM symbiosis in soil biogeochemical processes, by highlighting the ability of the AMF and accompanying microbiota in releasing significant amounts of LMWOAs to mobilize P bound to Fe oxides.