RESUMEN
The mimicking of the architectonics of native tissue, biodegradable non-woven fibrous mats is one of the most promising forms of scaffolding for tissue engineering. The key properties needed for their successful application in vivo, such as biodegradability, biocompatibility, morphology, mechanical properties, etc., rely on their composition and appropriate 3D structure. A multicomponent system based on biodegradable synthetic (polycaprolactone, oligo-/polylactide) and natural (chitosan, gelatin) polymers, providing the desired processing characteristics and functionality to non-woven mats fabricated via the electrospinning technique, was developed. The solid-state reactive blending of these components provided a one-step synthesis of amphiphilic graft copolymer with an ability to form stable ultra-fine dispersions in chlorinated solvents, which could be successfully used as casting solvents for the electrospinning technique. The synthesized graft copolymer was analyzed with the aim of fractional analysis, dynamic laser scattering, FTIR-spectroscopy and DSC. Casting solution characteristics, namely viscosity, surface tension, and electroconductivity, as well as electrospinning parameters, were studied and optimized. The morphology, chemical structure of the surface layer, mechanical properties and cytocompatibility were analyzed to confirm the appropriate functionality of the formed fibrous materials as scaffolds for tissue engineering.
RESUMEN
Graft copolymers of chitosan with cellulose ether have been obtained by the solid-state reactive mixing of chitin, sodium hydroxide and hydroxyethyl cellulose under shear deformation in a pilot twin-screw extruder. The structure and composition of the products were determined by elemental analysis and IR spectroscopy. The physicochemical properties of aqueous solutions of copolymers were studied as a function of the composition, and were correlated to the mechanical characteristics of the resulting films to assess the performance of new copolymers as coating materials, non-woven fibrous materials or emulsifiers for interface stabilization during the microparticle fabrication process.