Connectomic comparison of mouse and human cortex.

The human cerebral cortex houses 1000 times more neurons than that of the cerebral cortex of a mouse, but the possible differences in synaptic circuits between these species are still poorly understood. We used three-dimensional electron microscopy of mouse, macaque, and human cortical samples to study their cell type composition and synaptic circuit architecture. The 2.5-fold increase in interneurons in humans compared with mice was compensated by a change in axonal connection probabilities and therefore did not yield a commensurate increase in inhibitory-versus-excitatory synaptic input balance on human pyramidal cells. Rather, increased inhibition created an expanded interneuron-to-interneuron network, driven by an expansion of interneuron-targeting interneuron types and an increase in their synaptic selectivity for interneuron innervation. These constitute key neuronal network alterations in the human cortex.

Usage of social media in epidemic intelligence activities in the WHO, Regional Office for the Eastern Mediterranean.

Social media platforms are a massive source of information being used for monitoring and detecting various actual events such as natural disasters and disease outbreaks. This paper aims to present the experience of WHO, Regional Office for the Eastern Mediterranean in using social media for the detection and monitoring of COVD-19 pandemic alongside the other event-based surveillance tools. Over the period of 29 January 2020 to 31 May 2021, information was collected from social media and other media outlets (web news) as being the source of health information for early detecting and monitoring the situation of COVID-19 events. Signals were categorised into new events and event updates; where event updates captured from social media were categorised into official and unofficial. A total of 10 160 COVID-19 information were captured, out of which 95.8% (n=9732) were detected through social media. None of the information captured were discarded. 50.0% (n=11) of the COVID-19 events in the Eastern Mediterranean Region (EMR) were primarily captured from social media compared with 4.5% (n=1) primarily captured from other media outlets. Almost all (99.4%) of the event updates captured from social media were official updates. Real-time, transparent and relevant information posted on different social media platforms, especially the governmental official social media accounts, strengthened the early detection and follow-up of public health events in the EMR, especially during the current COVID-19 pandemic.

Echinococcosis in Tambool, Central Sudan: a knowledge, attitude and practice (KAP) study.

Introduction: In Sudan, echinococcosis (EC) is a chronic neglected zoonotic parasitic disease caused by Echinococcus granulosus. Studies have shown high prevalence rates in dogs (50-70%), camels (35%) and sheep, goats and cattle (10-11%). In total, 0.3-1.0% of humans in Central and South Sudan are infected with the G6 camel strain. This strain is almost exclusively the cause of human infections. The objective of this study was to explore knowledge, attitudes and practices (KAP) regarding the disease among people living around Tambool city, Central Sudan. Methods: A cross-sectional survey was conducted in three villages around the city of Tambool in Central Sudan. Three-hundred-and-twelve households were selected from the administrative unit of the area for participation in the study, of which 300 agreed to partake. A standardized questionnaire was designed to collect data on EC in animals, humans and the environment. The questionnaire domains were socio-demographic characteristics, KAP regarding echinococcosis. Results: The population surveyed showed that 68.7% (206/300) had never heard of the disease, while 31.3% (94/300) had heard about it. The level of knowledge among the 31.3% of those that had heard about the disease was excellent (69/94; 73.4%); so were their attitudes (76/94; 80.9%). However, the majority of the participants (64/94; 68%) showed poor practice regarding this disease, enhancing the odds for further propagation of parasite circulation in the animal and human populations at risk. Knowledge was found to be significantly associated with marital status. Practice was found to be significantly associated with occupation. Conclusions: There is a need for the implementation of a multidisciplinary program using the One Health approach to effectively control and prevent EC.

Distinct pathways of cholesterol biosynthesis impact on insulin secretion.

Results from previous investigations have indicated that glucose-stimulated insulin secretion (GSIS) is affected by changes in cholesterol and its intermediates, but the precise link between secretion and cholesterol has not been thoroughly investigated. In this study, we show the contribution of both protein isoprenylation and cholesterol-dependent plasma membrane structural integrity to insulin secretion in INS-1E cells and mouse islets. Acute (2 h) inhibition of hydroxyl-methylglutaryl-CoA reductase by simvastatin (SIM) resulted in inhibition of GSIS without reduction in total cellular cholesterol content. This effect was prevented by cell loading with the isoprenyl molecule geranylgeranyl pyrophosphate. Chronic (24 h) inhibition of cholesterol biosynthesis resulted in inhibition of GSIS with a significant reduction in total cellular cholesterol content, which was also observed after the inhibition of cholesterol biosynthesis downstream of isoprenoid formation. Electron paramagnetic resonance analyses of INS-1E cells showed that the SIM-induced reduction in cholesterol increased plasma membrane fluidity. Thus, the blockade of cholesterol biosynthesis resulted in the reduction of availability of isoprenoids, followed by a reduction in the total cholesterol content associated with an increase in plasma membrane fluidity. Herein, we show the different contributions of cholesterol biosynthesis to GSIS, and propose that isoprenoid molecules and cholesterol-dependent signaling are dual regulators of proper ß-cell function.