RESUMEN
Two amide-imine conjugates, viz. 3-methyl-benzoic acid (4-diethylamino-2-hydroxy-benzylidene)-hydrazide (L1) and 3-methyl-benzoic acid (2-hydroxy-naphthalen-1-ylmethylene)-hydrazide (L2), have been prepared and used for a further synthesis of Mo(vi) complexes (M1 and M2, respectively). Single crystal X-ray diffraction analysis confirmed their structures. Interestingly, M1 selectively recognizes Y3+ and Pb2+ at two different wavelengths, whereas M2 selectively interacts with Y3+ with a significantly high binding constant, 1.3 × 105 M-1. The proposed sensing mechanism involves the displacement of Mo(vi) by Y3+/Pb2+ from respective Mo(vi) complexes. The TCSPC experiment also substantiates the "turn-on" fluorescence process. A logic gate has been constructed utilizing the fluorescence recognition of cations by M1. DFT studies corroborated the cation-probe interactions and allowed exploring the orbital energy parameters.
RESUMEN
A new amide-imine conjugate, 2-hydroxybenzoic acid-(2-hydroxybenzylidene)-hydrazide (L1), is employed to prepare a single crystal X-ray structurally characterized poly-nuclear Cu(ii) complex (M1). M1 selectively and spatially interacts with cytochrome C (Cyt C) to allow fluorescence imaging of intracellular translocation events in living cells. Thus, direct visualization of a Cyt C translocation event during an apoptotic process is achieved for the first time. The binding constant and LOD are 7.52 × 104 M-1 and 34.0 nM, respectively.
Asunto(s)
Complejos de Coordinación/química , Cobre/química , Citocromos c/metabolismo , Mitocondrias/metabolismo , Animales , Apoptosis/efectos de los fármacos , Línea Celular , Complejos de Coordinación/farmacología , Citocromos c/análisis , Humanos , Hidrazinas/química , Ratones , Microscopía Fluorescente , Mitocondrias/efectos de los fármacos , EspectrofotometríaRESUMEN
A dinuclear Fe(iii) complex (F1) of an imine derivative (L1) derived from 3-ethoxy-2-hydroxy-benzaldehyde and hydrazine, structurally characterised via single crystal X-ray studies, is employed for the catalytic conversion of epoxides to cyclic carbonates utilizing carbon dioxide. In addition, F1 is employed for the selective optical recognition of nano-molar levels of Zn2+ (42.23 nM) via a metal displacement approach. The Job plot reveals interactions between F1 and Zn2+ at a 1 : 3 molar ratio with an association constant of 7.71 × 104 M-1. Studies on the catecholase-like activity of F1 reveal a k cat value of 4.42 × 103 h-1.
RESUMEN
Excited-state intra-molecular proton transfer (ESIPT)-active imine and azine derivatives, structurally characterised by XRD, and denoted L1, L2, L3 and L4, possess weak fluorescence. The interaction of these probes with Zn2+ turns ON the fluorescence to allow its nano-molar detection. Among the four ESIPT-active molecules, L2, L3 and L4 are bis-imine derivatives while L1 is a mono-imine derivative. Among the three bis-imine derivatives, one is symmetric (L3) while L2 and L4 are unsymmetrical. The lowest detection limits (DL) of L1, L2, L3 and L4 for Zn2+ are 32.66 nM, 36.16 nM, 15.20 nM and 33.50 nM respectively. All the probes bind Zn2+ (105 M-1 order) strongly. Computational studies explore the orbital level interactions responsible for the associated photo-physical processes.
