RESUMEN
Corynebacterium spp. are part of the human microbiome, but can cause the development of inflammatory diseases of various localization. Purpose - to evaluate the relationship between pathogenic properties and resistance to antimicrobial drugs (AMD) of Corynebacterium spp. from patients with inflammatory diseases of the respiratory tract. Strains of Corynebacterium spp. isolated from patients with inflammatory diseases of the respiratory tract (99 pcs.) and practically healthy individuals (33 pcs.). Isolates were identified by mass spectrometric method (MALDI-ToFMS), their adhesive and invasive activity on Hep-2 cells, cytopathic effect (CPE) in CHO-K1 cell culture, and resistance to antimicrobial drugs (AMD) were determined. Indicators of adhesion (3.65±0.679(CFU±m)x102/ml), invasion (1.72±0.230 (CFU±m)x102/ml), cytotoxicity (69.1±3.8% of dead CHO-K1 cells ) Corynebasterium spp. strains isolated from patients are higher (p≤0.05) than similar indicators in practically healthy people. 90.9% of isolates from patients had resistance to AMD, in most cases (57.6±4.9%) resistance to only one AMP was noted, less often to two (25.2±4.3%), three or more (8.08±2.7%). According to the results of correlation-regression analysis, pathogenic properties (adhesiveness, invasiveness, cytotoxicity) of Corynebacterium spp. strains isolated from patients are in close direct relationship with resistance to AMD. This indicates the importance of identifying strains of non-diphtheria corynebacteria resistant to AMDs, which, under the influence of developing resistance to AMDs, can increase their pathogenic potential, moving from commensalism to parasitism.
Asunto(s)
Antiinfecciosos , Infecciones por Corynebacterium , Antibacterianos/farmacología , Antibacterianos/uso terapéutico , Antiinfecciosos/farmacología , Corynebacterium , Infecciones por Corynebacterium/tratamiento farmacológico , Infecciones por Corynebacterium/microbiología , Farmacorresistencia Bacteriana/genética , HumanosRESUMEN
Determination of changes in the morphostructure of microorganisms under the influence of antibiotics, disinfectants, biocins, physical and chemical agents is carried out using the method of visualization of ultrathin sections in a transmission electron microscope. The aim is to develop methodological techniques for express preparation and visualization of the ultrastructure of bacteria in a transmission electron microscope to detect structural damage to cells and assess the quality of the microbial population. A technique of accelerated sample preparation for visualization of the fine structure of bacteria and diagnosis of structural damage to cells in a transmission electron microscope has been developed. The use of this method will make it possible to more effectively investigate the mechanisms of inactivation of microorganisms at different stages of contact with antibiotics, disinfectants, biocines, including various stressful, damaging and lethal physical and chemical effects.
Asunto(s)
Bacterias , Desinfectantes , Antibacterianos , Humanos , Microscopía Electrónica de Transmisión , Manejo de EspecímenesRESUMEN
In the post-epidemic period, the circulation of the causative agent of diphtheria in the population is maintained due to the carrier of bacteria. Entering an organism with a high level of antitoxic immunity, the pathogen enters into intermicrobial interactions with representatives of the opportunistic microflora inhabiting the respiratory tract and forms a biofilm. MATERIALS AND METHODS: Modeling of the biofilm formation process was carried out using the strains C.diphtheriae gravis tox+â. 665, C.pseudodiphtheriticum, S.aureus. Biofilm samples were placed on the stage of a scanning electron microscope and gold-sputtered in an EicoIB-3 ioncoater vacuum deposition unit (Eico, Japan) at an ion current of 6-8 mA. The samples obtained were examined in a JEOL 6510LB scanning electronmicroscope. («JEOL¼ company, Japan) at an accelerating voltage of 30 kV. RESULTS: Electron microscopic examination of samples of biofilms C. diphtheriae gravis tox+ â 665 and opportunistic microorganisms shows groups of 2-7 young bacterial cells packed into a single microcapsule. Much more voluminous accumulations of bacterial cells (more than 10-12) are typical for biofilm samples represented by C. diphtheriae gravis tox+â 665 and S. aureus cells. On the surface of the biofilm, young bacterial cells with an intact structure are located at various stages of active division. The conglomerates of bacterial cells, covered with a common intermicrobial matrix, adhere tightly to each other and form a multilayer biofilm. CONCLUSION: Features of the ultrastructure of biofilms containing strains of C. diphtheriae and opportunistic bacteria, especially antibiotic-resistant bacteria inhabiting the respiratory tract, can contribute to long-term persistence of the pathogen of diphtheria in the body. They not only significantly complicate the access of antibacterial drugs, but also interfere with the isolation of C.diphtheriae during bacteriological research.
Asunto(s)
Corynebacterium diphtheriae , Difteria , Biopelículas , Humanos , Sistema Respiratorio , Staphylococcus aureusRESUMEN
Corynebacterium spp. - representatives of the normal microflora of the human body, but their role in the development of diseases in both immunocompromised and immunocompetent patients is known. Corynebacterim spp. (C. pseudodiphtheriticum, C. striatum, C. amycolatum, C. accolens, C. argentoratense, etc.) is associated with diseases of the respiratory tract: tracheitis, pharyngitis, rhinosinusitis, bronchitis, etc. They can be transmitted by airborne droplets, household contact, and possibly by hematogenic pathways. Corynebacterim spp. toxins do not produce, but are capable of adhesion and invasion, biofilm formation, production of neuraminidase, hyaluronidase, and hemolysin. It is necessary to take into account not so much the species, but the strain affiliation of isolates of Corynebacterium spp., since among the representatives of one species of non-diphtheria corynebacteria (for example, C. pseudodiphtheriticum), colonizing the respiratory tract, there may be strains that can exhibit not only pathogenic properties, but also probiotic activity. Microbiological diagnostics is based on their quantitative determination in biological material, phenotypic (culture study, test systems for biochemical identification, Vitek 2 automated systems) and genotypic (16SpRNA gene sequencing and rpoB) methods. It is possible to use mass spectrometric analysis (MALDI-ToF-MS). The greatest activity against Corynebacterium spp. in vitro studies preserve vancomycin, teicoplanin, and linezolid. Successful therapy with at least two of the following antimicrobial agents (AMP) has been reported: vancomycin, rifampicin, linezolid, and daptomycin. The sensitivity of isolates of Corynebacterium spp. to AMP is not related to the species, but is due to strain differences, and therefore it is necessary to test each isolated strain. Continuous monitoring of the sensitivity of Corynebacterium spp. strains to AMP is necessary due to the observed variability of these traits. Of particular importance is the identification of multidrug-resistant isolates that are currently considered highly pathogenic. When compiling the review, the databases Scopus, Web of Science, The Cochrane Library, CyberLeninka, RSCI were used.