Exploring the potential of laser-textured metal alloys: Fine-tuning vascular cells responses through in vitro and ex vivo analysis.

Medical stents are vital for treating vascular complications and restoring blood flow in millions of patients. Despite its widespread effectiveness, restenosis, driven by the complex interplay of cellular responses, remains a concern. This study investigated the reactions of vascular cells to nano/microscale wrinkle (nano-W and micro-W) patterns created on laser-textured nitinol (NiTi) surfaces by adjusting laser processing parameters, such as spot overlap ratio and line overlap ratio. Evaluation of topographical effects on endothelial and smooth muscle cells (SMCs) revealed diverse morphologies, proliferation rates, and gene expressions. Notably, microscale wrinkle patterns exhibited reduced monocyte adhesion and inflammation-related gene expression, demonstrating their potential applications in mitigating vascular complications after stent insertion. Additionally, an ex vivo metatarsal assay was utilized to bridge the gap between in vitro and in vivo studies, demonstrating enhanced angiogenesis on laser-textured NiTi surfaces. Laser-textured NiTi exhibits a guided formation process, emphasizing their potential to promote swift endothelialization. These findings underscore the efficacy of laser texturing for tailored cellular interactions on metallic surfaces and offer valuable insights into optimizing biocompatibility and controlling cellular responses, which may pave the way for innovative advances in vascular care and contribute to the ongoing improvement of stent insertion.

Spatiotemporal control of neutrophil fate to tune inflammation and repair for myocardial infarction therapy.

Neutrophils are critical mediators of both the initiation and resolution of inflammation after myocardial infarction (MI). Overexuberant neutrophil signaling after MI exacerbates cardiomyocyte apoptosis and cardiac remodeling while neutrophil apoptosis at the injury site promotes macrophage polarization toward a pro-resolving phenotype. Here, we describe a nanoparticle that provides spatiotemporal control over neutrophil fate to both stymie MI pathogenesis and promote healing. Intravenous injection of roscovitine/catalase-loaded poly(lactic-co-glycolic acid) nanoparticles after MI leads to nanoparticle uptake by circulating neutrophils migrating to the infarcted heart. Activated neutrophils at the infarcted heart generate reactive oxygen species, triggering intracellular release of roscovitine, a cyclin-dependent kinase inhibitor, from the nanoparticles, thereby inducing neutrophil apoptosis. Timely apoptosis of activated neutrophils at the infarcted heart limits neutrophil-driven inflammation, promotes macrophage polarization toward a pro-resolving phenotype, and preserves heart function. Modulating neutrophil fate to tune both inflammatory and reparatory processes may be an effective strategy to treat MI.

Robust and customizable spheroid culture system for regenerative medicine.

Three-dimensional cell spheroids show promise for the reconstruction of native tissues. Herein, we report a sophisticated, uniform, and highly reproducible spheroid culture system for tissue reconstruction. A mesh-integrated culture system was designed to precisely control the uniformity and reproducibility of spheroid formation. Furthermore, we synthesized hexanoyl glycol chitosan, a material with ultralow cell adhesion properties, to further improve spheroid formation efficiency and biological function. Our results demonstrate improved biological function in various types of cells and ability to generate spheroids with complex structures composed of multiple cell types. In conclusion, our spheroid culture system offers a highly effective and widely applicable approach to generating customized spheroids with desired structural and biological features for a variety of biomedical applications.

A Current Development of Energy Harvesting Systems for Energy-Independent Bioimplantable Biosensors.

Biosensors have emerged as vital tools for the detection and monitoring of essential biological information. However, their efficiency is often constrained by limitations in the power supply. To address this challenge, energy harvesting systems have gained prominence. These off-grid, independent systems harness energy from the surrounding environment, providing a sustainable solution for powering biosensors autonomously. This continuous power source overcomes critical constraints, ensuring uninterrupted operation and seamless data collection. In this article, a comprehensive review of recent literature on energy harvesting-based biosensors is presented. Various techniques and technologies are critically examined, including optical, mechanical, thermal, and wireless power transfer, focusing on their applications and optimization. Furthermore, the immense potential of these energy harvesting-driven biosensors is highlighted across diverse fields, such as medicine, environmental surveillance, and biosignal analysis. By exploring the integration of energy harvesting systems, this review underscores their pivotal role in advancing biosensor technology. These innovations promise improved efficiency, reduced environmental impact, and broader applicability, marking significant progress in the field of biosensors.

Recent advancements in implantable neural links based on organic synaptic transistors.

The progress of brain synaptic devices has witnessed an era of rapid and explosive growth. Because of their integrated storage, excellent plasticity and parallel computing, and system information processing abilities, various field effect transistors have been used to replicate the synapses of a human brain. Organic semiconductors are characterized by simplicity of processing, mechanical flexibility, low cost, biocompatibility, and flexibility, making them the most promising materials for implanted brain synaptic bioelectronics. Despite being used in numerous intelligent integrated circuits and implantable neural linkages with multiple terminals, organic synaptic transistors still face many obstacles that must be overcome to advance their development. A comprehensive review would be an excellent tool in this respect. Therefore, the latest advancements in implantable neural links based on organic synaptic transistors are outlined. First, the distinction between conventional and synaptic transistors are highlighted. Next, the existing implanted organic synaptic transistors and their applicability to the brain as a neural link are summarized. Finally, the potential research directions are discussed.

Removal of tetramethylammonium hydroxide (TMAH) by cold plasma treatment combined with periodate oxidation: Degradation, kinetics, and toxicity study.

Tetramethylammonium hydroxide (TMAH), which is a chemical used in the electronic industry, is classified as a hazardous material (HAZMAT class 8) that threatens aquatic ecosystems and human health. Consequently, numerous studies have attempted to remove TMAH using various treatment methods, including advanced oxidation processes such as ozone, UV, or Fenton oxidation. However, prior research has indicated a low kinetic rate of TMAH removal. In this context, we proposed an alternative to TMAH degradation by combining a cold plasma (CP) process with periodate oxidation. As for the kinetics of TMAH removal, the kinetic constant was improved by 5 times (0.1661 and 0.0301 for 40.56 and 2.2 W, respectively) as the electric power of a CP system increased from 2.2 to 40.56 W. The kinetic constant of a 40.56 W CP system further increased by 54 times (1.6250) than a 2 W CP system when 4 mM periodate was used simultaneously. As a result, the integrated CP/periodate system represented 2 times higher TMAH removal efficiency (29.5%) than a 2 W CP system (14.4%). This excellent TMAH degradation capability of the integrated CP/periodate system became pronounced at pH 10 and 25 °C. Overall, the integrated CP/periodate system is expected to be a viable management option for effectively controlling hazardous TMAH chemicals.

Radiological safety assessment for transportation of reactor pressure vessel during decommissioning of a nuclear power plant in Korea.

In Korea, decommissioning of nuclear power plants and transportation of the decommissioning waste are expected to expand in the near future. It is necessary to confirm that radiological risks to the public and workers are not significant through radiological safety assessment. The objective of this study is to assess the radiological safety for transportation of RPV waste, which is a major decommissioning waste with relatively high level of radioactivity. It was assumed that the waste would be transported to the Gyeongju disposal facility by land transportation. First, the source term and transportation method of the RPV waste were determined, and the external dose rates from the waste were calculated using MCNP. Then, transportation scenarios were assumed under both normal and accident conditions. Under the scenarios, radiation doses were calculated using the RADTRAN. Under normal operation scenarios without a transportation accident, assuming 40 shipments per year, the average individual doses for the public ranged from 6.56×10-6to 2.18×10-2mSv yr-1. The maximum individual doses for only a single shipment ranged from 2.43×10-6to 3.14×10-1mSv. For cargo handlers and vehicle crew members, the average doses were 2.26×101mSv yr-1and 2.95 mSv yr-1, respectively. Under transportation accident scenarios, average individual radiological risks which are product of the radiation doses and the annual accident rates ranged from 1.14×10-11to 1.61×10-10mSv yr-1by transportation route segment when considering the transportation accident rate. Average individual doses assuming transportation accident occurrence ranged from 2.62×10-4to 1.42×10-3mSv. The maximum individual dose under accident conditions was 7.99×10-2mSv. The calculated doses were below the regulatory limits in Korea. However, relatively high doses were observed for cargo handlers and vehicle crew members because of conservative assumptions. This study results can be used as basic data for the radiological safety assessment for the decommissioning waste transportation in the future.

Versatile human cardiac tissues engineered with perfusable heart extracellular microenvironment for biomedical applications.

Engineered human cardiac tissues have been utilized for various biomedical applications, including drug testing, disease modeling, and regenerative medicine. However, the applications of cardiac tissues derived from human pluripotent stem cells are often limited due to their immaturity and lack of functionality. Therefore, in this study, we establish a perfusable culture system based on in vivo-like heart microenvironments to improve human cardiac tissue fabrication. The integrated culture platform of a microfluidic chip and a three-dimensional heart extracellular matrix enhances human cardiac tissue development and their structural and functional maturation. These tissues are comprised of cardiovascular lineage cells, including cardiomyocytes and cardiac fibroblasts derived from human induced pluripotent stem cells, as well as vascular endothelial cells. The resultant macroscale human cardiac tissues exhibit improved efficacy in drug testing (small molecules with various levels of arrhythmia risk), disease modeling (Long QT Syndrome and cardiac fibrosis), and regenerative therapy (myocardial infarction treatment). Therefore, our culture system can serve as a highly effective tissue-engineering platform to provide human cardiac tissues for versatile biomedical applications.

Dupo: A Mixed-Initiative Authoring Tool for Responsive Visualization.

Designing responsive visualizations for various screen types can be tedious as authors must manage multiple chart versions across design iterations. Automated approaches for responsive visualization must take into account the user's need for agency in exploring possible design ideas and applying customizations based on their own goals. We design and implement Dupo, a mixedinitiative approach to creating responsive visualizations that combines the agency afforded by a manual interface with automation provided by a recommender system. Given an initial design, users can browse automated design suggestions for a different screen type and make edits to a chosen design, thereby supporting quick prototyping and customizability. Dupo employs a two-step recommender pipeline that first suggests significant design changes (Exploration) followed by more subtle changes (Alteration). We evaluated Dupo with six expert responsive visualization authors. While creating responsive versions of a source design in Dupo, participants could reason about different design suggestions without having to manually prototype them, and thus avoid prematurely fixating on a particular design. This process led participants to create designs that they were satisfied with but which they had previously overlooked.

Numerical Study on Overcoming the Light-Harvesting Limitation of Lead-Free Cs2AgBiBr6 Double Perovskite Solar Cell Using Moth-Eye Broadband Antireflection Layer.

Lead-free Cs2AgBiBr6 double perovskite has emerged as a promising new-generation photovoltaic, due to its non-toxicity, long carrier lifetime, and low exciton binding energies. However, the low power conversion efficiency, due to the high indirect bandgap (≈2 eV), is a challenge that must be overcome and acts as an obstacle to commercialization. Herein, to overcome the limitations through the light trapping strategy, we analyzed the performance evaluation via FDTD simulation when applying the moth-eye broadband antireflection (AR) layer on top of a Cs2AgBiBr6 double perovskite cell. A parabola cone structure was used as a moth-eye AR layer, and an Al2O3 (n: 1.77), MgF2 (n: 1.38), SiO2 (n: 1.46), and ZnO (n: 1.9) were selected as investigation targets. The simulation was performed assuming that the IQE was 100% and when the heights of Al2O3, MgF2, SiO2, and ZnO were 500, 350, 250, and 450 nm, which are the optimal conditions, respectively, the maximum short-circuit current density improved 41, 46, 11.7, and 15%, respectively, compared to the reference cell. This study is meaningful and innovative in analyzing how the refractive index of a moth-eye antireflection layer affects the light trapping within the cell under broadband illumination until the NIR region.

Progress in organic photovoltaics for indoor application.

Organic photovoltaics (OPVs) have recently emerged as feasible alternatives for indoor light harvesting because of their variable optical absorption, high absorption coefficients, and low leakage currents under low lighting circumstances. Extensive research has been performed over the last decade in the quest for highly efficient, ecologically stable, and economically feasible indoor organic photovoltaics (IOPVs). This research covers a wide range of topics, including the development of new donor-acceptor materials, interlayers (such as electron and hole transport layers), energy loss reduction, open-circuit voltage enhancement via material and device engineering, and device architecture optimization. The maximum power conversion efficiency (PCE) of IOPVs has already topped 35% as a consequence of these collaborative efforts. However, further research is needed to improve numerous elements, such as manufacturing costs and device longevity. IOPVs must preserve at least 80% of their initial PCE for more than a decade in order to compete with traditional batteries used in internet of things devices. A thorough examination of this issue is urgently required. We intend to present an overview of recent developments in the evolution of IOPVs.

Role of quorum sensing and quorum quenching in anaerobic digestion: A scoping review.

Anaerobic digestion (AD) is a biological process that employs anaerobic microorganisms to degrade organic material, yielding biogas and biofertilizers. Understanding quorum sensing (QS) signaling in mixed microbial systems provides valuable insights into microbial behavior and functions. This review aims to examine recent studies on the roles of QS and QQ in the AD processes. A QS signal molecule, N-acyl homoserine lactone (AHL), induce the production of extraceluller polymers, promoting biofilm formation and bacterial aggregation, thereby the efficiency of AD process. QS-assisted granule formation fosters syntrophy between acetogens and methanogens, leading to increased organic removal and methane production. Specific AHLs were shown to be correlated with the abundance of hydrolytic bacteria and acidogens, further benefiting methane production. QQ was shown to effectively control membrane fouling in anaerobic membrane bioreactors, yet its impact on methane productivity remains unclear. This review shed lights on the existing literature gaps regarding the mechanisms of QS and QQ in AD systems, which will play a vital role in advancing AD applications in the future.

Vascular regeneration and skeletal muscle repair induced by long-term exposure to SDF-1α derived from engineered mesenchymal stem cells after hindlimb ischemia.

Despite recent progress in medical and endovascular therapy, the prognosis for patients with critical limb ischemia (CLI) remains poor. In response, various stem cells and growth factors have been assessed for use in therapeutic neovascularization and limb salvage in CLI patients. However, the clinical outcomes of cell-based therapeutic angiogenesis have not provided the promised benefits, reinforcing the need for novel cell-based therapeutic angiogenic strategies to cure untreatable CLI. In the present study, we investigated genetically engineered mesenchymal stem cells (MSCs) derived from human bone marrow that continuously secrete stromal-derived factor-1α (SDF1α-eMSCs) and demonstrated that intramuscular injection of SDF1α-eMSCs can provide long-term paracrine effects in limb ischemia and effectively contribute to vascular regeneration as well as skeletal muscle repair through increased phosphorylation of ERK and Akt within the SDF1α/CXCR4 axis. These results provide compelling evidence that genetically engineered MSCs with SDF-1α can be an effective strategy for successful limb salvage in limb ischemia.

Inhibiting Biofilm Formation via Simultaneous Application of Nitric Oxide and Quorum Quenching Bacteria.

Membrane biofouling is an inevitable challenge in membrane-based water treatment systems such as membrane bioreactors. Recent studies have shown that biological approaches based on bacterial signaling can effectively control biofilm formation. Quorum quenching (QQ) is known to inhibit biofilm growth by disrupting quorum sensing (QS) signaling, while nitric oxide (NO) signaling helps to disperse biofilms. In this study, batch biofilm experiments were conducted to investigate the impact of simultaneously applying NO signaling and QQ for biofilm control using Pseudomonas aeruginosa PAO1 as a model microorganism. The NO treatment involved the injection of NONOates (NO donor compounds) into mature biofilms, while QQ was implemented by immobilizing QQ bacteria (Escherichia coli TOP10-AiiO or Rhodococcus sp. BH4) in alginate or polyvinyl alcohol/alginate beads to preserve the QQ activity. When QQ beads were applied together with (Z)-1-[N-(3-aminopropyl)-N-(n-propyl) amino]diazen-1-ium-1,2-diolate (PAPA NONOate), they achieved a 39.0% to 71.3% reduction in biofilm formation, which was substantially higher compared to their individual applications (16.0% to 54.4%). These findings highlight the significant potential of combining QQ and NO technologies for effective biofilm control across a variety of processes that require enhanced biofilm inhibition.

Decreasing Hydrogen Content within Zirconium Using Au and Pd Nanoparticles as Sacrificial Agents under Pressurized Water at High Temperature.

Decreasing hydride-induced embrittlement of zirconium-based cladding is a significant challenge for the successful dry storage of spent nuclear fuel. Herein, to radically minimize hydride-induced embrittlement, we used nanoparticles as sacrificial agents with a greater affinity than zirconium for hydrogen. Corrosion experiments in the presence of gold (Au) and palladium (Pd) nanoparticles under simulated pressurized water reactor (PWR) conditions revealed that the hydrogen content of the zirconium samples was remarkably reduced, with a maximum decrease efficiency of 53.9% using 65 nm Au and 53.8% using 50 nm Pd nanoparticles. This approach provides an effective strategy for preventing hydride-induced embrittlement of zirconium-based cladding.

Derivation of dose constraint for the general public around nuclear power plants with operational data of radioactive effluent releases.

For radiation protection optimization, ICRP proposed dose constraint as quantitative value for planned exposure situation based on representative person concept. The objective of this study is to derive dose constraints for the general public around nuclear power plants in Korea by applying representative person concept. The dose constraints for the general public around NPPs were derived through a total of six steps. The steps consisted of setting source terms, setting exposure pathways and scenarios, setting candidate groups for a critical group decision, setting habit data, calculating radiation doses, and proposing dose constraints. Through these steps, the radiation dose distribution of the general public around the NPPs was obtained, and dose constraints were proposed using the dose distribution. Radiation doses to the general public around all the Korea NPP sites ranged 1.63 × 10-2 to 1.32 × 10-1 mSv/y. Using the dose distribution, 0.15 mSv/y, 0.10 mSv/y, and 0.08 mSv/y were proposed as dose constraints. The dose constraint values derived in this study are proposals. Therefore, it is judged that the dose constraints should need furthermore discussion with regulators, licensees, and radiation protection experts considering societal and economic factors for radiation protection. The proposal for dose constraints developed in this study can be used to optimize radiation protection for the general public around the NPPs.

Evaluation of Nitrogen Oxide Reduction Performance in Permeable Concrete Surfaces Treated with a TiO2 Photocatalyst.

Fine dust, recently classified as a carcinogen, has raised concerns about the health effects of air pollution. Vehicle emissions, particularly nitrogen oxide (NOx), contribute to ultrafine dust formation as a fine dust precursor. A photocatalyst, such as titanium dioxide (TiO2), is a material that causes a catalytic reaction when exposed to light, has exceptional characteristics such as decomposition of pollutants, and can be used permanently. This study aimed to investigate NOx reduction performance by developing ecofriendly permeable concrete with photocatalytic treatment to reduce fine dust generated from road mobile pollution sources. Permeable concrete specimens containing an activated loess and zeolite admixture were prepared and subjected to mechanical and durability tests. All specimens, including the control (CTRL) and admixture, met quality standard SPS-F-KSPIC-001-2006 for road pavement. Slip resistance and permeability coefficient also satisfied the standards, while freeze-thaw evaluation criteria were met only by CTRL and A1Z1 specimens. NOx reduction performance of the permeable concrete treated with TiO2 photocatalyst was assessed using ISO standard and tank chambers. NOx reduction efficiency of up to 77.5% was confirmed in the permeable concrete specimen with TiO2 content of 7.5%. Nitrate concentration measurements indirectly confirmed photolysis of nitrogen oxide. Incorporating TiO2 in construction materials such as roads and sidewalks can improve the atmospheric environment for pedestrians near roads by reducing NOx levels through photocatalysis.

Evaluation of the NOx Reduction Performance of Mortars Containing Zeolite/Activated Red Clay Coated with a TiO2 Photocatalyst.

Globally, there is a growing concern about air pollution due to rapid industrialization and urbanization. Therefore, in this study, an experimental study was conducted to evaluate the performance of reducing nitrogen oxides, a precursor to fine dust, in mortars coated with a titanium dioxide (TiO2) photocatalyst, which has the effect of decomposing pollutants. In particular, in this study, zeolite and activated red clay were used as cement substitutes to improve the fine dust reduction performance of the TiO2 photocatalyst. A total of 14 different mixtures were designed, considering the substitution rates of zeolite and activated red clay (30%, 40%, and 50%) and the cement-fine aggregate ratio (1:2 and 1:3) as experimental variables. A TiO2 photocatalyst was employed in this study to evaluate the NOx reduction performance. As zeolite and activated red clay were added, the compressive strength and flexural strength of the mortars decreased by 15% to 60%, while the absorption rate increased by 5% to 16%. The NOx reduction efficiency of up to 67.4% was confirmed in the H50-3 specimen with the TiO2 catalyst. The NOx reduction performance of mortars with the TiO2 photocatalyst sprayed on their surface improved as the substitution ratio of zeolite and activated red clay increased. Additionally, it was confirmed that the NOx reduction effect of specimens using activated red clay was superior to those using zeolite. Therefore, through this study, it was confirmed that the NOx reduction performance of the TiO2 photocatalyst can be improved when zeolite and activated red clay are used as cement substitutes.

Three-dimensional heart extracellular matrix enhances chemically induced direct cardiac reprogramming.

Direct cardiac reprogramming has emerged as a promising therapeutic approach for cardiac regeneration. Full chemical reprogramming with small molecules to generate cardiomyocytes may be more amenable than genetic reprogramming for clinical applications as it avoids safety concerns associated with genetic manipulations. However, challenges remain regarding low conversion efficiency and incomplete cardiomyocyte maturation. Furthermore, the therapeutic potential of chemically induced cardiomyocytes (CiCMs) has not been investigated. Here, we report that a three-dimensional microenvironment reconstituted with decellularized heart extracellular matrix can enhance chemical reprogramming and cardiac maturation of fibroblasts to cardiomyocytes. The resultant CiCMs exhibit elevated cardiac marker expression, sarcomeric organization, and improved electrophysiological features and drug responses. We investigated the therapeutic potential of CiCMs reprogrammed in three-dimensional heart extracellular matrix in a rat model of myocardial infarction. Our platform can facilitate the use of CiCMs for regenerative medicine, disease modeling, and drug screening.

Washable Fabric Triboelectric Nanogenerators for Potential Application in Face Masks.

In order to counteract the COVID-19 pandemic by wearing face masks, we examine washable fabric-based triboelectric nanogenerators (FTENGs). We applied the flash-spun nonwoven fabric (FS fabric) into the FTENGs, comparing the melt-blown nonwoven fabric (MB fabric) based FTENGs, which is conventionally studied in the field of energy harvesting. For reusability, all our proposed FTENGs are systematically investigated by controlling the washing conditions. After washing, the degradation ratio of the obtained output voltage is found to be only 12.5% for FS FTENGs, compared to the ratio of about 50% for the typical MB FTENGs. A rather small degradation ratio for FS fabric cases has resulted from less changed fabric structure after washing due to more dense fabric nature. Additionally, in order to improve the electrical characteristics of FS FTENGs. Note that the output voltage of FTENGs exhibits as much as 600 V.