Sub-Frame Evaluation of Frame Synchronization for Camera Network Using Linearly Oscillating Light Spot.

Precisely evaluating the frame synchronization of the camera network is often required for accurate data fusion from multiple visual information. This paper presents a novel method to estimate the synchronization accuracy by using inherent visual information of linearly oscillating light spot captured in the camera images instead of using luminescence information or depending on external measurement instrument. The suggested method is compared to the conventional evaluation method to prove the feasibility. Our experiment result implies that the estimation accuracy of the frame synchronization can be achieved in sub-millisecond order.

Visualization Method for the Cell-Level Vesicle Transport Using Optical Flow and a Diverging Colormap.

Elucidation of cell-level transport mediated by vesicles within a living cell provides key information regarding viral infection processes and also drug delivery mechanisms. Although the single-particle tracking method has enabled clear analysis of individual vesicle trajectories, information regarding the entire cell-level intracellular transport is hardly obtainable, due to the difficulty in collecting a large dataset with current methods. In this paper, we propose a visualization method of vesicle transport using optical flow, based on geometric cell center estimation and vector analysis, for measuring the trafficking directions. As a quantitative visualization method for determining the intracellular transport status, the proposed method is expected to be universally exploited in various biomedical cell image analyses.

Numerical method for vesicle movement analysis in a complex cytoskeleton network.

The detection of the precise movement of a vesicle during transport in a live cell provides key information for the intracellular delivery process. Here we report a novel numerical method for analyzing three-dimensional vesicle movement. Since the vesicle moves along a linear cytoskeleton during the active transport, our method first detects the orientation and position of the cytoskeleton as a linear section based on angle correlation and linear regression, after noise reduction. Then, the precise vesicle movement is calculated using vector analysis, in terms of rotation angle and translational displacement. Using this method, various vesicle trajectories obtained via high spatiotemporal resolution microscopy can be understood..

Visual encoder: robust and precise measurement method of rotation angle via high-speed RGB vision.

In this paper, a new measurement method of the rotation angle of a rotor, which is named the 'visual encoder,' was proposed. This method is based on the principles of the vision-based method and the optical encoder, and realized by using a high-speed vision system. The visual encoder shows advantageous features such as non-contact, high-resolution and robustness against the free motion and the fluctuation of the rotation axis. A high resolution method to increase the measurement resolution was also suggested. The accuracy and the robustness of the visual encoder were confirmed through the experimental verifications, and the operation was possible at 6,000 rpm even under the fluctuation of rotation axis.