Investigation of an Indian Site with Mafic Rock for Carbon Sequestration.

The increasing extent of greenhouse gas emissions has necessitated the development of techniques for atmospheric carbon dioxide removal and storage. Various techniques are being explored for carbon storage including geological sequestration. The geological sequestration has various avenues such as depleted oil and gas reservoirs, coal-bed methane reservoirs, and mafic and ultramafic rocks. Different trapping mechanisms are in play in these subsurface storage systems. In these sequestration sites, the mafic and ultramafic rocks are best suited for long-term and effective sequestration as they comprise minerals, conducive for chemical alteration, forming stable carbonates. However, these sites often suffer from distinct disadvantages of injectivity issues due to their low permeability and porosity. This study investigates the potential of sequestration in the rock samples obtained from one such site located in India. The rock samples are first characterized using various techniques including X-ray fluorescence, X-ray diffraction, Raman spectroscopy, and field emission scanning electron microscopy (FESEM). The mineralogical characterization shows that the rock sample contains approximately 10% of diopside. The samples were put in the reactor chamber comprising CO2, which were then investigated using FESEM analysis. Additionally, a reservoir block simulation using commercial software was conducted with the representative minerals in the sample to evaluate the CO2 sequestration potential. The simulation result suggests the formation of magnesite which corresponds to a major part of CO2 mineral trapping. The reduced injectivity due to low porosity and permeability in this rock can be addressed using hydraulic fracturing. The geomechanical behavior of the rock sample for hydraulic fracturing is studied using the Brazilian disc test. The Monte Carlo-based uncertainty analysis was conducted using the tensile strength data of the sample. Results suggest that the most likely fracturing pressure is 2100 psi for this rock sample.

Investigation of Filtration and Shale Inhibition Characteristics of Chitosan-N-(2-hydroxyl)-propyl trimethylammonium Chloride as Drilling Fluid Additives.

Hydrated shale formations often lead to severe drilling problems and may lead to wellbore instability. These instabilities can result in issues such as bit balling, borehole collapse, formation damage, stuck pipe, and low drilling rates. Keeping these fundamental issues with drilling in shale formation in mind, this study is aimed at designing a water-based drilling fluid system for effective shale inhibition, ensuring enhanced wellbore stability and drilling efficiency. The designed mud system comprises a typical base fluid along with newly synthesized chitosan derivative chitosan-N-(2-hydroxyl)-propyl trimethylammonium chloride (HACC) as an additive. This additive was found to be soluble in water and conducive for shale inhibition. The derived product was characterized by field emission scanning electron microscopy, thermogravimetric analysis, and Fourier-transform infrared spectroscopy (FTIR). Various drilling fluid tests, including filtration and rheological experiments, were conducted to evaluate its proficiency as a drilling mud additive. The results showed improvement in rheological and filtration properties after hot rolling at 100 °C in comparison to a conventional shale inhibitor, polyethylenimine. As we increase the concentration of synthesized chitosan derivative from 0.3 to 1.5 w/v%, the filtration loss is reduced from 40% to 65% as compared to the base fluids. Shale recovery tests were also conducted using shale samples from an Indian field to assess its viability for field application. The addition of 0.3 to 1.5 w/v% chitosan derivative resulted in high shale recovery above 88% to 96% at 100 °C compared to polyethylenimine, which showed a change in recovery from 62% to 73%. HACC intercalates into clay platelets, reducing the interlayer spacing between particles and preventing clay from hydrating and swelling. This mechanism of inhibition is evaluated by X-ray diffraction, FTIR, and zeta potential analysis. This bolsters the hypothesis of using the synthesized chitosan derivative as a shale inhibitor.

Community engagement approaches for malaria prevention, control and elimination: a scoping review.

INTRODUCTION: Globally malaria programmes have adopted approaches to community engagement (ACE) to design and deliver malaria interventions. This scoping review aimed to understand, map, and synthesise intervention activities guided by ACE and implemented by countries worldwide for the prevention, control and elimination of malaria. METHODS: Three databases (Web of Science, Proquest, and Medline) were searched for peer-reviewed, primary studies, published in English between 1 January 2000 and 31 December 2022. Advanced Google was used to search for grey literature. The five levels of the International Association for Public Participation were used to categorise ACE - (1) Inform, (2) Consult, (3) involve, (4) Collaborate, and (5) Co-lead. Intervention activities were categorised as health education (HE), and/or health services (HS), and/or environmental management (EM). Outcomes were collected as knowledge, attitude, behaviour, help-seeking, health and HS and environment. Enablers and barriers were identified. Malaria intervention phases were categorised as (1) prevention (P), or (2) control (C), or (3) prevention and control (PC) or prevention, control and elimination (PCE). RESULTS: Seventy-five studies were included in the review. Based on ACE levels, most studies were at the inform (n=37) and involve (n=26) level. HE (n=66) and HS (n=43) were the common intervention activities. HE informed communities about malaria, its prevention and vector control. EM activities were effective when complemented by HE. Community-based HS using locally recruited health workers was well-accepted by the community. Involvement of local leaders and collaboration with local stakeholders can be enablers for malaria intervention activities. CONCLUSION: Involving local leaders and community groups in all stages of malaria prevention programmes is vital for successful interventions. Key elements of successful ACE, that is, consult, collaborate, and co-lead were under-represented in the literature and require attention. National programes must consult and collaborate with community stakeholders to develop ownership of the interventions and eventually co-lead them.

Tiny titans- unravelling the potential of polysaccharides and proteins based dissolving microneedles in drug delivery and theranostics: A comprehensive review.

In recent years, microneedles (MNs) have emerged as a promising alternative to traditional drug delivery systems in transdermal drug delivery. The use of MNs has demonstrated significant potential in improving patient acceptance and convenience while avoiding the invasiveness of traditional injections. Dissolving, solid, hollow, coated, and hydrogel microneedles are among the various types studied for drug delivery. Dissolving microneedles (DMNs), in particular, have gained attention for their safety, painlessness, patient convenience, and high delivery efficiency. This comprehensive review primarily focuses on different types of microneedles, fabrication methods, and materials used in fabrication of DMNs such as hyaluronic acid, chitosan, alginate, gelatin, collagen, silk fibroin, albumin, cellulose and starch, to list a few. The review also provides an exhaustive discussion on the applications of DMNs, including the delivery of vaccines, cosmetic agents, contraceptives, hormone and genes, and other therapeutic applications like for treating cancer, skin diseases, and diabetes, among others, are covered in this review. Additionally, this review highlights some of the DMN systems that are presently undergoing clinical trials. Finally, the review discusses current advances and trends in DMNs, as well as future prospective directions for this ground-breaking technology in drug delivery.

Recent advances in the liposomal nanovesicles based immunotherapy in the treatment of cancer: A review.

Immunotherapy, along with chemotherapy, targeted delivery, radiation and surgery has become one of the most common cancer treatments. The aim of cancer immunology is to use the bodys immune system to combat tumors and develop a robust antitumor immune response. In the last few years, immune checkpoint inhibitors and chimeric antigen receptor-modified T cells have made substantial advancements in cancer immunotherapy. By boosting cell type-specific delivery and immunological responses, nanocarriers like liposomes have the ability to enhance greater immune responses. The efficacy of anti-tumor therapeutics is being significantly improved as liposomes can assist in resolving a number of issues that can arise from a variety of cancer immunotherapies. Since, liposomes can be loaded with both hydrophilic and hydrophobic drugs and protect the immunotherapeutic agents loaded inside the core, they offer significant advantages over other nano delivery systems. The use of liposomes for accurate and timely delivery of immunotherapies to particular targeted neoplasms, with little or no injury to healthy cells, maximizes immunotherapy efficacy. Liposomes are also suitable vehicles for delivering medications simultaneously with other therapies such as chemotherapy, radiation, and phototherapy. Liposomal nanoparticles will be introduced and used as an objective immunotherapy delivery system for great precision, making them a viable cancer treatment approach.With an emphasis on dendritic cells, T cells, tumor and natural killer cells, and macrophages; outline of many forms of immune-therapies in oncology and cutting-edge advances in liposomal nanovesicles for cancer immunotherapy are covered in this review.

Canine Distemper Virus in Tigers (Panthera tigris) and Leopards (P. pardus) in Nepal.

From wild dogs (Lycaon pictus) in the Serengeti to tigers (Panthera tigris altaica) in the Russian Far East, canine distemper virus (CDV) has been repeatedly identified as a threat to wild carnivores. Between 2020 and 2022, six Indian leopards (P. pardus fusca) presented to Nepali authorities with fatal neurological disease, consistent with CDV. Here, we report the findings of a serosurvey of wild felids from Nepal. A total of 48 serum samples were tested, comprising 28 Bengal tigers (P. t. tigris) and 20 Indian leopards. Neutralizing antibodies were identified in three tigers and six leopards, equating to seroprevalences of 11% (CI: 2.8-29.3%, n = 28) and 30% (CI: 12.8-54.3%, n = 20), respectively. More than one-third of seropositive animals were symptomatic, and three died within a week of being sampled. The predation of domestic dogs (Canis lupus familiaris) has been posited as a potential route of infection. A comparison of existing diet studies revealed that while leopards in Nepal frequently predate on dogs, tigers do not, potentially supporting this hypothesis. However, further work, including molecular analyses, would be needed to confirm this.

Comparative Study of the Effective Properties of 0-3 and Gyroid Triply Periodic Minimal Surface Cement-Piezocomposites.

In the present numerical simulation work, effective elastic and piezoelectric properties are calculated and a comparative study is conducted on a cement matrix-based piezocomposite with 0-3 and gyroid triply periodic minimal surface (TPMS) inclusions. The present study compares the effective properties of different piezoelectric materials having two different types of connectivity of the inclusions namely, 0-3 inclusions where the inclusions are physically separated from each other and are embedded within the matrix and the second one is TPMS inclusions having interpenetrating phase type connectivity. Effective properties are calculated for four different materials at five different volume fractions namely, 10%, 15%, 20%, 25%, and 30% volume fractions of inclusion by volume. In terms of effective properties and direct piezoelectric effect, TPMS piezocomposite is found to perform better compared to 0-3 piezocomposite. Lead-free piezoelectric material 0.5Ba(Ca0.8Zr0.2)O3 - 0.5(Ba0.7Ca0.3)TiO3 demonstrates better performance compared to all other material inclusions studied. The present study attempts to highlight improved piezoelectric effective properties of lead-free material-based piezocomposites with TPMS inclusions.

Conductive polymers and composites-based systems: An incipient stride in drug delivery and therapeutics realm.

Novel therapies and drug delivery systems (DDS) emphasis on localized, personalized, triggered, and regulated drug administration have heavily implicated electrically responsive DDS. An ideal DDS must deliver drugs to the target region at therapeutically effective concentrations to elicit a pharmacological response, resulting in better prophylaxis of the disease and the treatment. Biodegradable polymers are frequently employed for in-vivo long-term release; however, dose dumping can be anticipated. As a result, current DDSs can be tagged as dubbed "Smart Biomaterials" since they only focus on an on-demand cargo release in response to a trigger or stimulation. These organic materials have been recognized for their metal-like conductivity, as well as their mechanical stability and ease of production. These biomaterials can be programmed to respond to both internal and external stimuli. External pulsed triggers are required for extrinsic stimuli-responsive materials, whereas intrinsic stimuli-responsive materials rely on localized changes in the tissue environment. Furthermore, these materials have the ability to deliver active pharmaceutical agents at a varied concentration levels and across a broad spectrum of action. Drug delivery, biomedical implant technology, biosensor technology, and tissue engineering can be listed as a few prominent applications that have sparked immense interest for conductive polymers-based research and advancements in academia as well as in industry. This review comprehensively covers a cutting-edge collection of electrically conductive polymers and composites, and provide detailed insights of recent trends and advancements allied to conductive polymers for their potential applicability in an array of diverse meadows primarily focusing on drug delivery, biosensing and therapeutics. Furthermore, progressions in their synthesis, structural and functional properties have been presented in conjunction with futuristic directions for the smooth clinical translations.

In silico studies disclose the underlying link between binding affinity and redox potential in laccase isoforms.

Laccases are copper-containing enzymes belonging to the family of multicopper oxidases (MCOs). All MCOs use molecular oxygen to oxidize a wide range of organic compounds by radical catalysis. One of the key fundamental properties of laccases is having high or low redox potentials depending on the active site organization. Several experimental studies have been done to rationalize the high and low redox potential laccases (LRPL), however, molecular understanding is still lacking. In this work, we explored the proteomic profile of laccases produced in the fungal cultures, specifically induced with lignocellulosic biomass such as rice straw. This study was undertaken to explain the differences in the high redox and low redox potential values of different laccases using in-silico approaches. Proteomic profiling and structural and sequence analysis revealed a low level of similarity among them. Docking analyses and molecular dynamics simulation analysis revealed that high redox potential laccases (HRPL) are having good binding affinity compared to low or medium redox potential laccases (MRPL). The stability of these complexes was further analyzed based on reactive distances, active site volume comparison and a number of tunnel formations that were observed to be significantly higher for HRPL. Our results indicate that the number of tunnel formations calculated from the simulation's trajectories and available water molecules at the T3 site directly correlates with the laccases' redox potentials. This study will be helpful and provide valuable inputs for the designing of new laccases to improve lignin degradation.Communicated by Ramaswamy H. Sarma.

An Eye Tracking Based Framework for Safety Improvement of Offshore Operations.

Offshore drilling operations consist of complex and high-risk processes. Lack of situational awareness in drilling operations has become an important human factor issue that causes safety accidents. Prolonged work shifts and fatigue are some of the crucial issues that impact performance. Eye tracking technology can be used to distinguish the degree of awareness or alertness of participants that might be related to fatigue or onsite distractions. Oculomotor activity can be used to obtain visual cues that can quantify the drilling operators' situational awareness that might enable us to develop warning alarms to alert the driller. Such systems can help reduce accidents and save non-productive time. In this paper, eye movement char-acteristics were investigated to differentiate the situational awareness between a representa-tive expert and a group of novices using a scenario-based Virtual Reality Drilling Simulator. Significant visual oculomotor activity differences were identified between the expert and the novices that indicate an eye-tracking based system can detect the distraction and alert-ness exhibited by the workers. Results show promise on developing a framework which implements a real-time eye tracking technology in various drilling operations at drilling rigs and Real Time Operation Centers to improve process safety.

Traditional Beliefs, Practices, and Migration: A Risk to Malaria Transmission in Rural Nepal.

The study aimed to explore sociocultural factors influencing the risk of malaria and practices and beliefs towards malaria prevention, transmission and treatment in a remote village in Khatyad Rural Municipality (KRM) of Nepal. A sequential exploratory mixed methods approach was used. Qualitative data were collected through 25 one-on-one, in-depth interviews followed by a face-to-face household survey (n = 218) among people from a village in KRM believed to have a high risk of malaria. Traditional practices such as Chhaupadi requiring the seclusion of women during menstruation and post-partum, transhumance, and reliance on traditional healers for the management of malaria were common practices in the village. The household survey found 98.1% of women faced menstrual exile either inside the house or in a separate hut, with 64.2% not having access to Long-lasting Insecticidal Nets (LLINs). Hardships and economic constraints compelled villagers to migrate seasonally for work to malaria-endemic areas in India, thereby exposing themselves to the risk of malaria. Persistent traditional beliefs and seasonal migration could threaten the elimination goals set by the national malaria program.

Negative Poisson's ratio polyethylene matrix and 0.5Ba(Zr0.2 Ti0.8) O3-0.5(Ba0.7 Ca0.3)TiO3 based piezocomposite for sensing and energy harvesting applications.

Finite element studies were conducted on 0.5Ba(Zr0.2 Ti0.8) O3-0.5(Ba0.7 Ca0.3)TiO3 (BCZT) piezoelectric particles embedded in polyethylene matrix to create a piezocomposite having a positive and negative Poisson's ratio of -0.32 and 0.2. Polyethylene with a positive Poisson's ratio is referred to as non-auxetic while those with negative Poisson's ratio are referred to as auxetic or inherently auxetic. The effective elastic and piezoelectric properties were calculated at volume fractions of (4%, 8% to 24%) to study their sensing and harvesting performance. This study compared lead-free auxetic 0-3 piezocomposite for sensing and energy harvesting with non-auxetic one. Inherently auxetic piezocomposites have been studied for their elastic and piezoelectric properties and improved mechanical coupling, but their sensing and energy harvesting capabilities and behavior patterns have not been explored in previous literatures. The effect of Poisson's ratio ranging between -0.9 to 0.4 on the sensing and energy harvesting performance of an inherently auxetic lead free piezocomposite composite with BCZT inclusions has also not been studied before, motivating the author to conduct the present study. Auxetic piezocomposite demonstrated an overall improvement in performance in terms of higher sensing voltage and harvested power. The study was repeated at a constant volume fraction of 24% for a range of Poisson's ratio varied between -0.9 to 0.4. Enhanced performance was observed at the extreme negative end of the Poisson's ratio spectrum. This paper demonstrates the potential improvements by exploiting auxetic matrices in future piezocomposite sensors and energy harvesters.

Targeting Magnetic Nanoparticles in Physiologically Mimicking Tissue Microenvironment.

Magnetic nanoparticles as drug carriers, despite showing immense promises in preclinical trials, have remained to be only of limited use in real therapeutic practice primarily due to unresolved anomalies concerning their grossly contrasting controllability and variability in performance in artificial test benches as compared to human tissues. To circumvent the deficits of reported in vitro drug testing platforms that deviate significantly from the physiological features of the living systems and result in this puzzling contrast, here, we fabricate a biomimetic microvasculature in a flexible tissue phantom and demonstrate distinctive mechanisms of magnetic-field-assisted controllable penetration of biocompatible iron oxide nanoparticles across the same, exclusively modulated by tissue deformability, which has by far remained unraveled. Our experiments deciphering the transport of magnetic nanoparticles in a blood analogue medium unveil a decisive interplay of the flexibility of the microvascular pathways, magnetic pull, and viscous friction toward orchestrating the optimal vascular penetration and targeting efficacy of the nanoparticles in colorectal tissue-mimicking bioengineered media. Subsequent studies with biological cells confirm the viability of using localized magnetic forces for aiding nanoparticle penetration within cancerous lesions. We establish nontrivially favorable conditions to induce a threshold force for vascular rupture and eventual target of the nanoparticles toward the desired extracellular site. These findings appear to be critical in converging the success of in vitro trials toward patient-specific targeted therapies depending on personalized vascular properties obtained from medical imaging data.

Development of multiplex PCR assay for detection of Alternaria brassicae, A. brassicicola and Xanthomonas campestris pv. campestris in crucifers.

Among biotic stresses, Alternaria leaf spots caused by Alternaria brassicae and A. brassicicola and black rot caused by Xanthomonas campestris pv. campestris are major limiting factors in brassica cultivation across the world. Because of seed-borne nature of these pathogens primarily, disease-free conservation as well as exchange of brassica seeds at domestic as well as international level are major challenges. To facilitate disease-free conservation and transboundary movement of brassica germplasm, a highly specific and sensitive method was developed for simultaneous detection of these pathogens. A set of primers namely, AbeABC1F and AbeABC1R based on ABC transporter (Atr1) gene for A. brassicae, Aba28sF and Aba28sR based on SSR marker was developed for A. brassicicola as well as rpf gene-based primers namely, rpfH_F and rpfH_R for X. campestris pv. campestris were used for multiplex PCR. The specific bands of 586, 201 and 304 bp were obtained in multiplex PCR assay for A. brassicae, A. brassicicola and X. campestris pv. campestris, respectively. Therefore, the developed multiplex PCR protocol could be utilized for a reliable diagnosis of these pathogens to facilitate safe conservation, exchange of seeds to the researchers and also by seed certification agencies for ensuring quality seed availability to farmers.

A qualitative study of knowledge, attitudes and perceptions towards malaria prevention among people living in rural upper river valleys of Nepal.

BACKGROUND: Nepal has made significant progress in decreasing the number of malaria cases over the last two decades. Prevention and timely management of malaria are critical for the National Malaria Program in its quest for elimination. The study aimed to explore the knowledge, attitudes and behaviour towards malaria prevention and treatment among people living in rural villages of Khatyad Rural Municipality in Nepal. METHODS: This qualitative study collected information through virtual in-depth interviews (N = 25) with female and male participants aged between 15 and 72 years. RESULTS: More than half of the participants knew about the causes of malaria, were aware of the complications of untreated malaria and knew that anti-malarial medicines were provided for free at the public health facilities. Participants indicated that their first choice of health care were public health facilities, however limited supply of medications and diagnostics deviated patients to the private sector. While tertiary care costs were not financially viable, participants opted against traditional care for malaria. Factors such as cost of treatment, distance to the health facility and the decision making authority in households influenced health related decisions in the family. Although long-lasting insecticidal nets were distributed and indoor residual spraying was done periodically, several barriers were identified. CONCLUSION: Increased awareness of malaria prevention and treatment among people living in malaria risk areas is important for the National Malaria Program in its quest for malaria elimination in Nepal.

Community engagement approaches for malaria prevention, control and elimination: a scoping review protocol.

Community engagement (CE) is important for malaria prevention, control and ultimately elimination. As the decline of malaria has plateaued over the last 5 years, strengthening CE approaches will be necessary to enhance health promotion practice and policy to drive malaria transmission down further. Countries have adopted a wide range of public health intervention approaches for malaria prevention and control that best suit their context. This review will examine the existing evidence on the various CE approaches adopted by malaria programmes across the world and their outcomes. METHODOLOGY AND ANALYSIS: The review methodology will follow the updated Joanna Briggs Institute guide for scoping review, 2017, which is based on the framework developed by Arksey and O'Malley and further developed by Levac Colquhoun and O'Brien. Proquest, Web of Knowledge and Medline will be searched for publications from January 2000 to 31 March 2021 while Google search engine will be used to find any grey literature. The eligibility criteria will be as follows: review will include primary studies written in the English language using appropriate study designs and methods, including quantitative, qualitative and mixed methods designs; and case, programme or project reports. Information on CE approaches designed specifically for malaria prevention, control, elimination and their outcomes will be explored. Subheadings and free text terms for 'community engagement' and 'malaria' will be used for the search. The article screening and data extraction will be examined by two reviewers after the initial search, and any disputes will be resolved by a third reviewer through discussion. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for scoping reviews guide will be used to present the review methods and the results from the search. The scoping review results will identify and map the available evidences, sources of information and research gaps in the area of CE as one approach for malaria prevention, control and/or elimination. ETHICS AND DISSEMINATION: This study only aims to review secondary sources and does not require human research ethics committee approval. The findings of the scoping review will be submitted to a peer-reviewed journal for wider dissemination.

COVID-19 control strategies and intervention effects in resource limited settings: A modeling study.

INTRODUCTION: Many countries with weaker health systems are struggling to put together a coherent strategy against the COVID-19 epidemic. We explored COVID-19 control strategies that could offer the greatest benefit in resource limited settings. METHODS: Using an age-structured SEIR model, we explored the effects of COVID-19 control interventions-a lockdown, physical distancing measures, and active case finding (testing and isolation, contact tracing and quarantine)-implemented individually and in combination to control a hypothetical COVID-19 epidemic in Kathmandu (population 2.6 million), Nepal. RESULTS: A month-long lockdown will delay peak demand for hospital beds by 36 days, as compared to a base scenario of no intervention (peak demand at 108 days (IQR 97-119); a 2 month long lockdown will delay it by 74 days, without any difference in annual mortality, or healthcare demand volume. Year-long physical distancing measures will reduce peak demand to 36% (IQR 23%-46%) and annual morality to 67% (IQR 48%-77%) of base scenario. Following a month long lockdown with ongoing physical distancing measures and an active case finding intervention that detects 5% of the daily infection burden could reduce projected morality and peak demand by more than 99%. CONCLUSION: Limited resource settings are best served by a combination of early and aggressive case finding with ongoing physical distancing measures to control the COVID-19 epidemic. A lockdown may be helpful until combination interventions can be put in place but is unlikely to reduce annual mortality or healthcare demand.

Wetting, Adhesion, and Droplet Impact on Face Masks.

In the present pandemic time, face masks are found to be the most effective strategy against the spread of the virus within the community. As aerosol-based spreading of the virus is considered as the primary mode of transmission, the interaction of masks with incoming droplets needs to be understood thoroughly for an effective usage among the public. In the present work, we explore the interactions of the droplets over the most commonly used 3-ply surgical masks. A detailed study of the wetting signature, adhesion, and impact dynamics of water droplets and microbe-laden droplets is carried out for both sides of the mask. We found that the interfacial characteristics of the incoming droplets with the mask are very similar for the front and the back side of the mask. Further, in an anticipated attempt to reduce the adhesion, we have tested masks with a superhydrophobic coating. It is found that a superhydrophobic coating may not be the best choice for a regular mask as it can give rise to a number of smaller daughter droplets that can linger in air for longer times and can contribute to the transmission of potential viral loads.

Microparticle Suspensions and Bacteria-Laden Droplets: Are They the Same in Terms of Wetting Signature?

Adhesion behavior of microbial pathogens on commonly encountered surfaces is one of the most pertinent questions now. We present the characterization of bacteria-laden droplets and quantify the adhesion forces on highly repellent surfaces with the help of a simple experimental setup. Comparing the force signature measured directly using an in-house capillary deflection-based droplet force apparatus, we report an anomalous adhesion behavior of live bacteria (E. coli)-laden droplets on repellent surfaces, which stands in stark contrast to the observed adhesion signature when the doping agent is changed to inert microparticles or the same bacteria in an incapacitated state. We showed that the regular contact angle measurements using optical goniometry is unable to differentiate between the live bacteria and the dead ones (including microparticles) and thus delineate its limitations and the complementary nature of the adhesion measurements in understanding the fundamental interfacial interaction of living organisms on solid surfaces.