Conformation and protein interactions of intramolecular DNA and phosphorothioate four-way junctions.

The objectives of this study are to evaluate the structure and protein recognition features of branched DNA four-way junctions in an effort to explore the therapeutic potential of these molecules. The classic immobile DNA 4WJ, J1, is used as a matrix to design novel intramolecular junctions including natural and phosphorothioate bonds. Here we have inserted H2-type mini-hairpins into the helical termini of the arms of J1 to generate four novel intramolecular four-way junctions. Hairpins are inserted to reduce end fraying and effectively eliminate potential nuclease binding sites. We compare the structure and protein recognition features of J1 with four intramolecular four-way junctions: i-J1, i-J1(PS1), i-J1(PS2) and i-J1(PS3). Circular dichroism studies suggest that the secondary structure of each intramolecular 4WJ is composed predominantly of B-form helices. Thermal unfolding studies indicate that intramolecular four-way junctions are significantly more stable than J1. The Tm values of the hairpin four-way junctions are 25.2° to 32.2°C higher than the control, J1. With respect to protein recognition, gel shift assays reveal that the DNA-binding proteins HMGBb1 and HMGB1 bind the hairpin four-way junctions with affinity levels similar to control, J1. To evaluate nuclease resistance, four-way junctions are incubated with DNase I, exonuclease III (Exo III) and T5 exonuclease (T5 Exo). The enzymes probe nucleic acid cleavage that occurs non-specifically (DNase I) and in a 5'→3' (T5 Exo) and 3'→5' direction (Exo III). The nuclease digestion assays clearly show that the intramolecular four-way junctions possess significantly higher nuclease resistance than the control, J1.

Lumbar spine evaluation: accuracy on abdominal CT.

OBJECTIVE: To determine if the lumbar spine can be accurately evaluated on an abdominal CT. METHODS: The electronic medical records at our institution were searched to find all consecutive patients who had an abdominal CT within 12 months of a lumbar spine MRI obtained between 01 November 2010 and 31 October 2015. The abdominal CT studies were retrospectively reviewed in a blinded fashion for the presence of any significant lumbar spine abnormalities. The prospective lumbar spine MRI reports were used as the standard of reference. RESULTS: 5,031 patients had lumbar spine MRI studies at our institution during the study period of 01 November 2010 to 31 October 2015. 144 patients met the inclusion criteria of our study. No patients were excluded. 107 patients had 256 abnormal findings on the lumbar spine MRI studies. The sensitivity, specificity, positive predictive value, negative predictive value and accuracy of abdominal CT in lumbar spine evaluation on a per patient/per finding basis were 89.7/95.3%, 97.3/100%, 99.0/99.2%, 76.6/99.8% and 91.7/99.8%, respectively. CONCLUSION: Despite several limitations (e.g. spinal cord assessment, bone marrow assessment and quantum mottle) compared with evaluation of the lumbar spine using MRI, evaluation of the lumbar spine on abdominal CT studies can be accurately performed with current state of the art CT scanners. Additional prospective studies are needed for a more definitive analysis. Advances in knowledge: With recent advances in CT technology, accurate evaluation of the lumbar spine on abdominal CT studies is feasible, potentially providing significant additional information to patients without additional imaging.

Reuse and reduce: abdominal CT, lumbar spine MRI, and a potential 1.2 to 3.4 billion dollars in cost savings.

PURPOSE: To determine how much money could potentially be saved by re-evaluating a patient's prior recent abdominal CT for lumbar spine pathology instead of ordering a lumbar spine MRI. METHODS: Abdominal CT studies, from all consecutive patients who had an abdominal CT within 12 months prior to a lumbar spine MRI obtained between 11/1/15 and 5/30/16, were retrospectively reviewed in a blinded fashion for the presence of any significant lumbar spine abnormalities. CT studies that accurately reflected all normal and abnormal findings when compared to the standard of reference, the prospectively interpreted lumbar spine MR imaging reports, were used to indicate which lumbar spine MRI studies potentially could have been avoided and to calculate the potential cost savings. RESULTS: Of the 81 abdominal CT studies that met the inclusion criteria of this study, 62% (50/81) were TP, 28% (23/81) were TN, 5% (4/81) were FP, and 5% (4/81) were FN studies. 90% (73/81) of the lumbar spine MRI studies could potentially have been avoided during the 7 months of this study. The predicted savings by reviewing the abdominal CT for lumbar spine abnormalities prior to ordering a lumbar spine MRI are an estimated 1.2-3.4 billion dollars per year. CONCLUSION: Recent abdominal CT studies should be reviewed for lumbar spine pathology prior to a patient undergoing lumbar spine MRI. Avoiding unnecessary lumbar spine MRI studies could potentially save the U.S. healthcare system an estimated 1.2-3.4 billion dollars per year.

Polyurethane foam (PUF) passive samplers for monitoring phenanthrene in stormwater.

Pollution from highway stormwater runoff has been an increasing area of concern. Many structural Best Management Practices (BMPs) have been implemented for stormwater treatment and management. One challenge for these BMPs is to sample stormwater and monitor BMP performance. The main objective of this study was to evaluate the feasibility of using polyurethane foam (PUF) passive samplers (PSs) for sampling phenanthrene (PHE) in highway stormwater runoff and BMPs. Tests were conducted using batch reactors, glass-tube columns, and laboratory-scale BMPs (bioretention cells). Results indicate that sorption for PHE by PUF is mainly linearly relative to time, and the high sorption capacity allows the PUF passive sampler to monitor stormwater events for months or years. The PUF passive samplers could be embedded in BMPs for monitoring influent and effluent PHE concentrations. Models developed to link the results of batch and column tests proved to be useful for determining removal or sorption parameters and performance of the PUF-PSs. The predicted removal efficiencies of BMPs were close to the real values obtained from the control columns with errors ranging between -8.46 and 1.52%. This research showed that it is possible to use PUF passive samplers for sampling stormwater and monitoring the performance of stormwater BMPs, which warrants the field-scale feasibility studies in the future.

Impact of improved air quality during the 1996 Summer Olympic Games in Atlanta on multiple cardiovascular and respiratory outcomes.

Substantial evidence supports an association between ambient air pollution, especially particulate matter (PM*) and ozone (O3), and acute cardiovascular and respiratory morbidity. There is increasing interest in accountability research to evaluate whether actions taken to reduce air pollution will result in reduced morbidity. This study capitalized on a unique opportunity to evaluate the impact of a local, short-term intervention effort to reduce traffic in Atlanta during the 1996 Summer Olympic Games (July 19-August 4). Air pollutant concentrations both inside and outside of Atlanta were examined during the Olympic period and surrounding periods. Emergency department (ED) visits were examined to evaluate changes in usage patterns. ED visits for respiratory and cardiovascular conditions were examined in relation to the Olympic period using Poisson time-series analysis with adjustment for time trends and meteorologic conditions. O3 concentrations were approximately 30% lower during the Olympic Games compared with the four weeks before and after the Olympic Games (baseline periods); however, we observed similar reductions in O3 concentrations in several other cities in the Southeastern United States. We observed little or no evidence of reduced ED visits during the Olympic Games; the estimates were sensitive to choice of analytic model and to method of adjusting for temporal trends. The meteorologic conditions during the Olympic Games, along with the reductions in O3 observed in various cities not impacted by the Olympic Games, suggest that both meteorologic conditions-and reduced traffic may have played a role in the observed reduction in O3 concentration in Atlanta. Additionally, it is likely that this particular intervention strategy would not be sustainable as a pollution-reduction strategy. This study demonstrates some limitations of conducting retrospective accountability research.