RESUMEN
Gilts that reach puberty at an earlier age with more backfat have greater lifetime productivity. Increased growth rates generally promote earlier age at first estrus; however, an association of age at first estrus with discrete measures of body fatness remains controversial. We tested the hypothesis that metabolic state as determined by concentrations of plasma urea nitrogen (PUN), which reflect lean tissue growth, were correlated with age at first estrus. Blood samples were collected from gilts (n = 337) at 102, 123, and 145 d of age during development. Concentrations of albumin, creatinine, glucose, and PUN were determined. Body weight and backfat thickness were determined at each time point. From 130 to 240 d of age, gilts were monitored for first pubertal estrus. Concentrations of creatinine increased whereas concentrations of glucose decreased with increasing age (P < 0.0001). Concentrations of albumin and PUN remained relatively stable throughout development. Average daily BW gain (r = 0.22) and change in backfat thickness (r = 0.29) had a positive phenotypic correlation (P < 0.0001) with PUN at 145 d of age. Concentrations of PUN at 102 and 123 d of age were not phenotypically correlated with pubertal age, but there was a moderately negative phenotypic correlation (r = -0.22; P < 0.0001) of PUN at 145 d of age with age at first estrus along with a negative genetic correlation (r = -0.42). The relationship of PUN with age at first estrus shifted from liner to quadratic with advancing age. These data demonstrate that near the age at which gilts are selected for entry into the breeding unit, those with greater PUN have increased BW and backfat thickness and display pubertal estrus earlier but that PUN does not account for additional variation in age at first estrus beyond growth rate or backfat. It is concluded that PUN can be used to select gilts with increased efficiency of nutrient use without negatively impacting pubertal development.
Asunto(s)
Composición Corporal , Estro , Nitrógeno/sangre , Maduración Sexual , Sus scrofa/fisiología , Urea/sangre , Animales , Nitrógeno de la Urea Sanguínea , Femenino , Sus scrofa/crecimiento & desarrolloRESUMEN
Leptin produced by adipocytes acts through leptin receptors in the hypothalamus to control appetite and food intake and thus communicates information about degree of fatness. It is thought that a degree of body fat is required for initiation of puberty and maintenance of reproductive function in mammals. The objective of this study was to determine whether polymorphisms in the leptin (LEP), leptin receptor (LEPR), paired box 5 (PAX5), aldo-keto reductase (AKR), and pro-opiomelanocortin (POMC) genes were associated with age, leptin concentration, backfat as an indicator of body condition, or BW at puberty in 3 lines of gilts and to characterize genetic relationships among these traits. The first 2 lines, born in 2001, were formed by crossing maternal White Cross (Yorkshire x Maternal Landrace) gilts to Duroc (n = 210) or (lean) Landrace (n = 207) boars. The remaining line (n = 507), born in 2002, was formed by crossing progeny of the Duroc- and Landrace-sired lines. At first estrus, age, BW (BWP), and backfat (BFP) at puberty were recorded and blood was collected for leptin assays. Nine SNP were detected in candidate genes/regions: 1 in LEP, 3 in LEPR, 1 in PAX5, 2 in AKR, and 2 in POMC. Animals were genotyped for each of the SNP; genotypes were validated using GenoProb. The association model included fixed effects of farrowing group, covariates of SNP genotypic probabilities (from GenoProb), and random additive polygenic effects to account for genetic similarities between animals not explained by SNP. Variance components for polygenic effects and error were estimated using MTDFREML. Leptin concentrations were logarithmically transformed for data analysis. All 4 traits were moderately to highly heritable (0.38 to 0.48). Age and leptin at puberty had a significant (P < 0.01) genetic correlation at -0.63 +/- 0.097, and the genetic correlation between BWP and age at puberty was 0.65 +/- 0.083 (P < 0.01). Significant additive associations (a; P < 0.05) were detected at PAX5 for age at puberty (a = 3.2 d) and for BFP (a = 0.61 mm). One SNP in LEPR was associated with leptin concentration (a = 0.31 log units; P < 0.05). The associations from PAX5 correspond to a QTL peak for age at puberty detected on SSC1. Although not necessarily the causative mutation, this result implies that a QTL that can decrease age at puberty without increasing BFP and BWP at puberty may exist in this region in commercial pigs.
Asunto(s)
Composición Corporal/genética , Leptina/sangre , Maduración Sexual/genética , Porcinos/genética , Tejido Adiposo/fisiología , Animales , Peso Corporal/genética , Femenino , Polimorfismo de Nucleótido Simple , Porcinos/fisiologíaRESUMEN
In pork production, the efficiency of dietary protein (AA) use is low, resulting in urinary excretion of large quantities of nitrogen as urea. Use of AA and formation of urea are under enzymatic regulation, suggesting genetic regulation. The current study examined the effects of sire line, sire, and sex on growth characteristics and plasma urea nitrogen (PUN) concentrations in the offspring of 11 Duroc sires and 11 Landrace sires bred to Yorkshire-Landrace dams. Plasma samples were obtained at approximately 107 (age class = 107 d), 128 (age class = 128 d), and 149 (age class = 149 d) d of age from 511 boars, gilts, and barrows group-penned and fed standard finishing diets. Body weight and backfat (BF, mean of 3 measurements) were recorded at the time of blood sample collection. Sex, age class, and their interaction influenced (P < 0.01) BW, BF, and PUN. Predicted traits (i.e., ADG, BW at 21 wk, average daily change in BF, BF at 21 wk, and the mean of 3 PUN measures) were generated. Means (+/-SD) were: ADG, 888 +/- 204 g; BW at 21 wk, 94.2 +/- 12.5 kg; average daily change in BF, 0.083 +/- 0.052 mm; BF at 21 wk, 13.8 +/- 3.0 mm; and the mean of 3 PUN measures, 16.2 +/- 4.4 mg/dL. Predicted weight traits were influenced (P < 0.05) by sire line, and sex influenced (P < 0.01) all predicted traits. Heritability estimates for PUN at 107, 128, and 149 d of age were 0.35 +/- 0.15, 0.21 +/- 0.13, and 0.16 +/- 0.12, respectively. Phenotypic correlations of PUN with growth and fat traits were low. Genetic correlations of PUN measured at 107 d with growth and fat traits were low. However, genetic correlations of PUN measured at 128 or 149 d with growth and fat traits ranged from 0.81 to 0.95. Determination of PUN, as herein, may be of sufficient precision to allow its use in a selection protocol. Selection of pigs with superior growth performance and low PUN may result in a greater efficiency of dietary nitrogen use and a reduced negative environmental impact.
Asunto(s)
Tejido Adiposo/anatomía & histología , Nitrógeno de la Urea Sanguínea , Composición Corporal/genética , Peso Corporal/genética , Caracteres Sexuales , Porcinos/genética , Alimentación Animal , Animales , Femenino , Masculino , Porcinos/crecimiento & desarrolloRESUMEN
To alleviate the need for daily injection of porcine somatotropin (pST), a sustained-release implant (pSTSR) was devised that continuously delivers a daily dose of 2 mg of pST for 42 d. Ninety-six white composite (Large White x Landrace) finishing barrows (83.6 +/- 1.2 kg BW) were assigned to receive zero or two pSTSR implants (4 mg pST/d) and to consume one of six diets differing in total Lys concentration (0.29, 0.52, 0.75, 0.98, 1.21, or 1.44%, as-fed basis). Diets were formulated to be isocaloric and based on the ideal protein concept. Pigs were housed individually, allowed ad libitum access to feed and water, and slaughtered at 112 kg of BW. The pSTSR affected neither ADG (P = 0.88) nor 10th rib LM area (LMA; P = 0.51), but it decreased (P < 0.01) ADFI, average backfat thickness, 10th rib fat depth, weights of leaf fat and ham fat, improved (P < 0.05) G:F, and increased (P < 0.01) weights of four trimmed lean cuts (T-cuts), and percentages of ham lean and bone. Increasing total Lys increased ADG (quadratic; P < 0.05) and ADFI (linear; P < 0.01). The G:F, plasma urea N concentrations (PUN), and T-cuts were affected by the interaction pSTSR x dietary Lys (P < 0.01). Without pSTSR, the G:F did not differ (P = 0.37) among pigs fed 0.52% and greater total Lys. With pSTSR, the G:F was less (P < 0.05) for pigs fed 0.52% than 0.98 and 1.44% total Lys. Increases in dietary total Lys resulted in increased PUN (P < 0.01), and incremental increases were less in pSTSR-implanted pigs. Maximal yield of T-cuts was at 0.98% dietary total Lys in nonimplanted pigs and 1.21% total Lys in pSTSR-implanted pigs. Estimates of total Lys requirements of pigs without and with pSTSR, respectively, were 0.52 and 0.86% for growth (ADG and G:F) and 0.73 and 0.88% for lean production (LMA and T-cuts). Equivalent apparent ileal digestible Lys requirements of pigs without and with pSTSR, respectively, were 0.44 and 0.68% for growth, and 0.62 and 0.75% for lean production. With ADFI of 3.5 kg daily, an intake of approximately 26.1 g of total daily Lys (0.75%) or 22.4 g of apparent ileal digestible Lys is needed to maximize lean production in finishing barrows receiving 4 mg pST/d via sustained-release implant.
Asunto(s)
Fenómenos Fisiológicos Nutricionales de los Animales , Hormona del Crecimiento/administración & dosificación , Hormona del Crecimiento/farmacología , Lisina/metabolismo , Necesidades Nutricionales , Porcinos/crecimiento & desarrollo , Implantes Absorbibles , Alimentación Animal , Animales , Composición Corporal/efectos de los fármacos , Preparaciones de Acción Retardada , Dieta , Masculino , Porcinos/metabolismo , Aumento de Peso/efectos de los fármacosRESUMEN
Objectives of this study were to evaluate effects of seven sire breed groups and three levels of daily ME intake (DMEI = 132 or 189 kcal ME/kg BW(0.75) or ad libitum), beginning 5 mo prepartum, on BCS, length of postpartum anestrus, and circulating concentrations of IGF-1 and GH in F1 cows (six to eight cows per sire breed in each DMEI group) out of Angus or Hereford dams. At the initiation of the study, BW were 522, 530, 548, 572, 575, 577, and 595 kg for cows sired by Longhorn, Galloway, 1960s Hereford or Angus, 1980s Hereford or Angus, or Nellore, Salers, and Shorthorn bulls, respectively (SE = 13; P < 0.001 for sire breed). After 4 mo on DMEI treatment during the pre-partum period, cows fed 132 kcal of ME/kg BW(0.75)gained little to no BW; cows fed 189 kcal ME/kg BW(0.75) gained 50 kg; and cows fed ad libitum gained 70 kg (all groups differ P < 0.05). Concentrations of progesterone in weekly blood samples collected 2 to 14 wk after calving were used to establish when normal luteal function resumed to predict length of postpartum anestrus. Length of anestrus was affected by level of DMEI in cows sired by Galloway, Longhorn, and Nellore bulls, but not other breeds (P < 0.02 for interaction of sire breed and DMEI). Level of DMEI, but not sire breed, affected (P < 0.01) BCS at wk 2 postpartum. Concentrations of IGF-1 at wk 2 postpartum differed (P < 0.001) due to sire breed, and changes in concentrations of IGF-1 from wk 2 to 14 were influenced (P < 0.03) by the interaction of sire breed and level of DMEI; which was primarily the result of differences in rate of decrease over time among different sire breed x level of DMEI groupings. Concentrations of GH did not differ due to sire breed but varied (P < 0.001) due to the interaction of DMEI and week postpartum, for which concentrations of GH did not differ at wk 2 but increased over time at rates that were inversely proportional to level of DMEI. Length of anestrus was negatively associated (P < 0.05) with day of calving, BCS, and BW. When effects of sire breed and level of DMEI were accounted for (residual correlation), length of anestrus was inversely associated (P < 0.01) with IGF-1 concentrations. Breed of sire influenced length of postpartum anestrus and energy balance, as predicted by IGF-1, in crossbred cows fed varying levels of DMEI.
Asunto(s)
Anestro/fisiología , Cruzamiento , Bovinos/fisiología , Ingestión de Energía/fisiología , Hormona del Crecimiento/fisiología , Factor I del Crecimiento Similar a la Insulina/fisiología , Alimentación Animal/análisis , Animales , Peso Corporal/fisiología , Dieta/veterinaria , Femenino , Hormona del Crecimiento/sangre , Factor I del Crecimiento Similar a la Insulina/análisis , Lactancia/fisiología , Análisis de los Mínimos Cuadrados , Masculino , Distribución Aleatoria , Estadística como Asunto , Factores de TiempoRESUMEN
The importance of birth-to-weaning average daily gain as a determinant of weight at a final age and yield of marketable pork was investigated. Treatments were imposed to create variation in birth-to-weaning ADG independent of birth weight. Newborn pigs were cross-fostered to create litters of four through 14 pigs/litter. Creep feed was offered to pigs from 5 d of age or during last 2 d before weaning at 13 to 20 d (average = 17 d). Growth rate and carcass dissection data were obtained from 195 barrows that were slaughtered at an average age of 170 d (SD = 7.5), weight of 109 kg (SD = 10.5). All traits measured were influenced by birth dam and sire (P < 0.01). Quadratic and cubic effects (P < 0.09) of litter size on birth-to-weaning ADG and weaning weight were different between the creep feeding treatments. Data revealed a positive influence (P < 0.04) of creep feeding from 5 d of age on birth-to-weaning ADG and weaning weight in larger size (> 8) litters. Importance of the independent variables birth weight, birth-to-weaning ADG, weaning weight, and birth weight plus birth-to-weaning ADG in determination of measures of postweaning growth and yield of marketable pork were examined by step-down regression analysis. Initial models included the linear and quadratic effects of the independent variables. In general, R2 for models ranked birth weight < birth-to-weaning ADG < d-17 weaning weight < birth weight + birth-to-weaning ADG. The R2 of models for BW at 170 d of age were 0.11 (P < 0.01) using birth weight as the independent variable, 0.16 (P < 0.01) using birth-to-weaning ADG, 0.19 (P < 0.01) using d-17 weaning weight, and 0.21 (P < 0.01) using birth weight + birth-to-weaning ADG. The model for effect of birth-to-weaning ADG on BW at 170 d of age indicated that a 10-g advantage in birth-to-weaning ADG produced a 0.94-kg advantage in BW at 170 d of age. Positive relationships (P < 0.05) between birth-to-weaning ADG and measures of postweaning growth and carcass yield suggest management practices that increase birth-to-weaning ADG may be advantageous in pork production.
Asunto(s)
Envejecimiento/fisiología , Alimentación Animal , Tamaño de la Camada/fisiología , Carne/normas , Porcinos/crecimiento & desarrollo , Aumento de Peso/fisiología , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Animales Recién Nacidos/crecimiento & desarrollo , Peso Corporal , Ingestión de Alimentos , Femenino , Masculino , Factores de Tiempo , DesteteRESUMEN
Chinese Meishan pigs develop rapidly with onset of puberty at less than 100 days of age, and have a smaller placental size and larger litter size as compared with British/Continental breeds. POU1F1 is a member of the POU-domain family gene and is a positive regulator for growth hormone (GH), prolactin (PRL), and thyroid-stimulating hormone beta (TSHbeta) in several mammalian species. To investigate the role of POU1F1 in controlling pig growth and reproduction traits, Meishan (MS) pigs segregating a MspI POU1F1 polymorphism were used to determine differences of GH and PRL at both mRNA and circulating hormone concentrations. Animals from nine litters were used to collect pituitary (n=60) and/or blood samples (n=80) at day 0, 15, and 30 after birth, and all animals were genotyped (CC, CD, DD) for the MspI POU1F1 polymorphism. Reverse transcriptase-polymerase chain reaction (RT-PCR) with standard curve quantification was used to quantify mRNA levels for GH, PRL, and two alternative POU1F1 transcripts, POU1F1-alpha, and POU1F1-beta. Radioimmunoassays were done to determine the circulating concentration of GH and PRL in blood plasma. Our results indicated a significant effect of POU1F1 genotype on circulating levels of both GH and PRL at birth, but not thereafter. The DD neonates had lower levels of GH, but higher levels of PRL, than other genotypes. POU1F1-alpha mRNA decreased (P<0.05) from days 0 to 30, which paralleled decreases (P<0.05) in GH mRNA as well as PRL and GH plasma levels over the same period. POU1F1-beta mRNA levels did not significantly change over this period. Correlations were significant between POU1F1-alpha mRNA and both GH mRNA and GH plasma concentration levels, as well as between the two POU1F1 mRNA isoforms. Results from this study add to our understanding of the role of POU1F1 in controlling pig development and reproduction.
Asunto(s)
Hormona del Crecimiento/sangre , Prolactina/sangre , Porcinos/genética , Factores de Transcripción/genética , Envejecimiento/fisiología , Animales , Animales Recién Nacidos , Femenino , Genotipo , Hormona del Crecimiento/genética , Tamaño de la Camada , Masculino , Polimorfismo Genético , Prolactina/genética , ARN Mensajero/análisis , Radioinmunoensayo/veterinaria , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/veterinaria , Porcinos/sangre , Porcinos/crecimiento & desarrollo , Tirotropina , Factores de Tiempo , Factores de Transcripción/fisiologíaRESUMEN
Development of gilts that conceive early and continue to produce offspring is a primary objective of swine production. The objective of this study was to determine the degree of feed restriction during development required to optimize reproductive performance and efficiency in gilts. The effects that various patterns of growth had on reproductive development and performance of gilts through d 30 of gestation were investigated. At 13 wk of age and 41 kg BW, 192 white crossbred gilts were penned individually and assigned to receive 87.5%, 75%, 62.5%, and 50% of predicted ad libitum energy intake. The study was replicated in two seasons. At 25 wk of age, gilts were moved to group pens and allowed ad libitum access to feed, and estrous detection was initiated. Gilts were inseminated at first observed estrus and those recycling were remated. Post-mating gilts were fed 1.5x maintenance in stalls. Gilts that did not return to estrus 17 to 30 d after mating were slaughtered at 30 d of gestation. Reproductive tracts were collected and numbers of corpora lutea and live embryos were recorded. Feed restriction during development resulted in differences in BW and backfat thickness at the start of the breeding period and differences in feed intake during breeding. Gilts subjected to the greatest feed restriction during development consumed the greatest quantity of feed during breeding. Feed intake during breeding was associated with BW and backfat gain during breeding. The treatment group that entered breeding lightest and leanest (50% of predicted ad libitum intake) had the least number of days to first estrus, followed by the fattest, heaviest group (87.5% of predicted ad libitum intake). Treatment groups did not differ (P > 0.38) in ovulation rate or live embryo numbers. Significant relationships between quantity of GE consumed during development and variables considered important in reproductive development and performance were evident, such as BW and fatness at start of breeding and first detected estrus, and ovulation rate. Variation in dietary energy during the development period impacted many aspects of reproductive development and performance. However, coupling restricted energy intake during development with ad libitum intake during breeding negated many of the effects of feed restriction during the development period.
Asunto(s)
Ingestión de Energía/fisiología , Privación de Alimentos/fisiología , Reproducción/fisiología , Maduración Sexual/fisiología , Porcinos/fisiología , Alimentación Animal , Animales , Peso Corporal , Cruzamiento , Estro/fisiología , Femenino , Embarazo , Distribución Aleatoria , Porcinos/crecimiento & desarrolloRESUMEN
Development of gilts that conceive early and continue to produce offspring is an objective of swine production. We investigated different patterns of growth on reproductive development and performance of gilts through the first farrowing. At 13 wk of age and 43 kg BW, 286 white crossbred gilts were penned individually and assigned to treatments: Ad lib, ad libitum intake from 13 to 25 wk of age; Control, ad libitum intake from 13 wk of age until 100 kg BW and then 90% of ad libitum intake until 25 wk of age; and Restricted, 74% of ad libitum intake from 13 wk to 25 wk of age. Feed was formulated to restrict energy intake. The study was replicated in three seasons. At 25 wk of age, gilts were moved by treatment to group pens, fed for ad libitum consumption, and estrus detection was initiated. Gilts were inseminated at first estrus, and those recycling were remated. Postmating gilts were fed 1.5x maintenance until 105 to 110 d of pregnancy. Gilts were moved either to the farrowing facility or the abattoir at 105 to 110 d of pregnancy. Those taken to the abattoir were slaughtered and number, weight, and condition of the fetuses were recorded. Gilts moved to the farrowing facility were allowed to farrow, and number, weight, and condition of the piglets were recorded. Daily feed intake during breeding was 3.4 kg/d by Restricted gilts, 2.9 by Control gilts, and 2.7 kg/d by Ad lib gilts. Increased feed intake by Restricted gilts during breeding resulted in compensatory gains that overcame the reduced reproductive performance that resulted from the reduced BW and backfat these gilts carried at the start of breeding. Days to first estrus and pregnancy were not influenced by development period treatment (P < 0.13). Percentage of Ad lib, Control, and Restricted gilts that successfully completed their pregnancies were 61, 74, and 66, respectively (P > 0.19). Total feed fed from 13 wk of age to end of the first pregnancy per gilt assigned did not differ among Ad lib (506 kg) and Control (498 kg) gilts but was less (P < 0.01) in Restricted gilts (451 kg). Number of piglets born per gilt assigned (P > 0.09) and piglets produced per kilogram of feed fed from 13 wk of age to term (P > 0.29) were 6.47 and 0.0134 in Ad lib gilts, 7.26 and 0.0150 in Control gilts, and 6.38 and 0.0149 in Restricted gilts, respectively. Moderate feed restriction, 74% of ad libitum intake, reduced feed consumed from 13 wk of age to end of the first pregnancy with no significant impact on efficiency of piglet production.
Asunto(s)
Alimentación Animal , Privación de Alimentos/fisiología , Reproducción/fisiología , Maduración Sexual/fisiología , Porcinos/crecimiento & desarrollo , Animales , Peso Corporal , Ingestión de Energía , Estro/fisiología , Femenino , Embarazo , Estaciones del AñoRESUMEN
Endocrine differences between European crossbred and Chinese Meishan females have been somewhat modest. Indwelling jugular cannulas were placed in Meishan (n = 7) and White composite (n = 6) multiparous sows before weaning, and blood was sampled from 4 h before to 240 h after removal of litters. Temporal changes in FSH, triiodothyronine (T3), and tetraiodothyronine (T4) after weaning differed between Meishan and White composite sows. Plasma cortisol concentrations were higher in Meishan sows than in White composite sows (P < 0.01), but there were no temporal differences between breeds after weaning. Other hormones monitored (prolactin, GH, IGF-I, and inhibin) were not different between breeds. In the second experiment, Meishan gilts (n = 7) and sows (n = 7) and White composite sows (n = 9) were cannulated during the luteal phase of the estrous cycle and sampled after treatments consisting of GnRH (15 and 150 ng/kg BW), ovariectomy, estradiol cypionate challenge after ovariectomy (10 microg/kg BW), and GnRH antagonist. In response to GnRH challenge, White composite sows had elevated (P < 0.05) concentrations of gonadotropins compared with Meishan. Cortisol concentrations were elevated in Meishan as compared with White composite females (P < 0.01) but unaffected by GnRH treatment. After ovariectomy, LH concentrations increased 3 h sooner in White composite than in Meishan females. After GnRH antagonist, declines in gonadotropins were comparable in both breeds, but LH increased in Meishan females by 20 h but not until after 54 h in White composite females. White composite females demonstrated only a short decline in FSH in response to the GnRH antagonist, but Meishan females had a prolonged decline in FSH concentrations. Consistently elevated cortisol concentrations in Meishan females may positively impact ovarian function; thus, the assumption that high plasma cortisol concentration as an index of stress that impairs reproductive function should be reevaluated in swine. There were few endocrine differences that would relate to or explain increased ovulation rates found in the Meishan breed; thus, other mechanisms must exist to explain the increase in ovarian function in the Meishan breed.
Asunto(s)
Fase Luteínica , Porcinos/fisiología , Destete , Animales , Estradiol/análogos & derivados , Estradiol/farmacología , Femenino , Hormona Folículo Estimulante/sangre , Hormona Liberadora de Gonadotropina/sangre , Hidrocortisona/sangre , Inhibinas/sangre , Hormona Luteinizante/sangre , Masculino , Ovariectomía , Ovulación , Especificidad de la Especie , Porcinos/clasificación , Tiroxina/sangre , Triyodotironina/sangreRESUMEN
The effect of prepubertal feed level on growth and reproductive development of gilts was investigated. At 13 wk. of age, white crossbred gilts were penned individually and assigned to the following treatments: Ad lib, ad libitum intake from 13 to 25 wk. of age (n = 64); Control, ad libitum intake from 13 wk. of age until 100 kg BW and then 90% of ad libitum intake until 25 wk. of age (n = 65); and Restricted, 74% of ad libitum intake from 13 wk. to 25 wk. of age (n = 64). Feed was formulated to primarily restrict energy intake. The study was replicated in two seasons. At 25 wk. of age, gilts were moved to group pens, approximately 16 gilts/pen, allowed ad libitum access to feed, and estrus detection was initiated. Gilts were mated at first estrus and those recycling were remated. After mating, gilts were moved to gestation stalls and fed 1.5x maintenance. At 30 d of gestation, reproductive tracts were harvested, and numbers of corpora lutea (CL) and live embryos were recorded. From 13 to 25 wk. of age, feed consumption was 258 for Ad lib, 251 for Control, and 189 kg/gilt for Restricted, and, from 13 wk. of age until 30 d of gestation, total feed consumption was 367 for Ad lib, 356 for Control, and 299 kg/gilt for Restricted gilts. Age at puberty (196 d) and pregnancy (200 d) was not affected (P>.18) by treatment. However, the rate at which gilts attained puberty (e.g., percentage pubertal at 28 d) was greatest in Ad lib (75) and least in Control (61) gilts. Number of CL and live embryos at 30 d of gestation/gilt assigned to the study was unaffected (P>.21) by treatment. Quantity of feed consumed from 13 wk. of age to 30 d of gestation per live embryo in gilts assigned to the study was 40.0 for Ad lib, 39.8 for Control, and 30.6 kg/gilt for Restricted gilts. These results indicate that moderate feed restriction of gilts during prepubertal development may increase efficiency of swine production without negative impact on reproductive performance through 30 d of gestation.
Asunto(s)
Alimentación Animal , Reproducción/fisiología , Maduración Sexual/fisiología , Porcinos/crecimiento & desarrollo , Animales , Ingestión de Alimentos , Femenino , EmbarazoRESUMEN
Antibody against porcine relaxin (antipRLX540; 1:950,000) was produced in sheep and used to determine the effect on relaxin and progesterone secretion, and on parturition in late pregnant pigs. In group 1, Yorkshire gilts with normal estrous cycles were bred on the second observed estrus and fitted with an indwelling jugular cannula and an intraperitoneal cannula on day 100 of pregnancy. Gilts were infused at 6-h intervals with antipRLX540 (n = 10) or PBS (n = 10) beginning on day 103 until parturition. From days 103 to 120, daily blood samples (10 ml) were collected for RIA of relaxin, progesterone, and prolactin. In group 2, bred gilts were randomly assigned to antipRLX540 (n = 11), relaxin (n = 5), and PBS (n = 8) treatment on days 111, 113, and 115. Blood was collected twice daily from day 108 to 120, and every 20 min on days 111, 113, and 115 beginning 60 min before treatment and continuing 180 min. Parturition in gilts given antipRLX540 occurred on day 112.7 compared with day 114.0 in relaxin-treated gilts and day 114.3 in PBS controls (P < 0.05). Duration of delivery from first to last piglet was greatly delayed in antipRLX540 gilts (240 min) compared with PBS controls ([117 min] P < 0.005). Average number of stillborns was greater in antipRLX540- than in PBS-treated controls (2.4 vs. 1.0; P < 0.05). Relaxin concentration in peripheral plasma was lower in antipRLX540-treated gilts from day 105 to 110, but on day 113 the antipRLX540-treated group had a greater relaxin peak release compared with PBS-treated animals (P < 0.05). Plasma progesterone concentrations were similar in antipRLX540- and PBS-treated gilts throughout the period of the study. In group 2, by day 113, progesterone decreased in antipRLX540-treated gilts compared with relaxin- and PBS-treated gilts. Prolactin levels were similar in both antipRLX540- and PBS-treated gilts; however, from 1 to 3 days postpartum the antipRLX540 group had higher prolactin concentration (P < 0.05). The results indicate that antipRLX540 decreased circulating plasma concentrations of unbound or free relaxin during the last 10 days of pregnancy in Yorkshire gilts. AntipRLX540 markedly increased both the duration of delivery of piglets and the average number of stillbirths in this litter-bearing species compared with PBS-treated controls. This study provides strong evidence that increasing circulating concentrations of relaxin during late pregnancy is crucial for unimpaired parturition in the pig.
Asunto(s)
Anticuerpos/farmacología , Trabajo de Parto/fisiología , Relaxina/metabolismo , Porcinos/fisiología , Animales , Animales Recién Nacidos/fisiología , Anticuerpos/uso terapéutico , Femenino , Muerte Fetal/epidemiología , Muerte Fetal/veterinaria , Sueros Inmunes/biosíntesis , Trabajo de Parto/inmunología , Masculino , Embarazo , Progesterona/sangre , Progesterona/metabolismo , Prolactina/sangre , Radioinmunoensayo/veterinaria , Distribución Aleatoria , Relaxina/sangre , Relaxina/inmunología , Ovinos , Porcinos/inmunologíaRESUMEN
The actions of IGF-I, alone and in combination with porcine growth hormone (pGH), on growth and circulating endocrines and metabolites important in growth were investigated in peripubertal-age Meishan barrows. Pigs were assigned to four treatments: control, buffer; IGF-I, 33 microg rhIGF-I/kg BW injected twice daily; pGH, 33 microg rpGH/kg BW injected once daily; and IGF-I+pGH, 33 microg rhIGF-I/kg BW injected twice daily plus 33 microg rpGH/kg BW injected once daily. Treatments were administered for 28 d. Feed intake, BW, and backfat were recorded and blood samples were collected weekly. At slaughter, organ and primal cut weights were recorded. Offal and half the carcass were ground for chemical analysis. Serum concentrations of IGF-I on d 7, 14, 21, and 28 in the IGF-I, pGH, and IGF-I+pGH groups were increased 60, 107, and 131%, respectively, compared with those of the control group. Administration of pGH increased gain 43%, feed efficiency 60%, carcass protein accretion 88%, and trimmed lean cuts 16%, whereas IGF-I administration increased gain 22%, carcass protein accretion 33%, and trimmed lean cuts 5%. There was little difference in responses to administration of IGF-I+pGH and pGH alone except that coadministration of IGF-I with pGH reduced the ability of pGH to suppress backfat gain (P < .02). Even though administration of IGF-I resulted in a 60% increase in chronic nadir serum concentrations of IGF-I, only a few growth and carcass measures were changed when compared with control pigs. These included increased (P < .05) weight of body, leaf fat, kidneys, and belly. The actions of pGH on growth of pigs were not mimicked, and some were countermanded by administration of IGF-I at a dose that produces significantly increased serum concentrations of IGF-I.
Asunto(s)
Composición Corporal/efectos de los fármacos , Glándulas Endocrinas/efectos de los fármacos , Hormona del Crecimiento/farmacología , Factor I del Crecimiento Similar a la Insulina/farmacología , Porcinos/crecimiento & desarrollo , Porcinos/metabolismo , Animales , Ingestión de Alimentos/efectos de los fármacos , Glándulas Endocrinas/metabolismo , Hormona del Crecimiento/administración & dosificación , Hormona del Crecimiento/sangre , Insulina/sangre , Proteínas de Unión a Factor de Crecimiento Similar a la Insulina/sangre , Factor I del Crecimiento Similar a la Insulina/administración & dosificación , Factor I del Crecimiento Similar a la Insulina/análisis , Factor II del Crecimiento Similar a la Insulina/análisis , Masculino , Porcinos/sangre , Factores de Tiempo , Aumento de Peso/efectos de los fármacosRESUMEN
Prostaglandins primarily of uterine origin play an important role in parturition. Hysterectomy of nongravid pigs early in the luteal phase maintains luteal function until about Day 150, whereas the duration of normal pregnancy is about 114 days. A precisely timed peak release of relaxin and coincident decrease in progesterone secretion in unmated hysterectomized gilts are similar to hormonal changes that occur a few hours before parturition. It is hypothesized that prostaglandin F2alpha (PGF2alpha) in hysterectomized pigs mimics abrupt changes in ovarian and pituitary hormone secretion seen before normal parturition and in early lactation. Unmated Yorkshire gilts were hysterectomized on Days 6-8 of a normal estrous cycle, and at 1200 h on Day 113, they were given an i.m. injection of 30 mg PGF2alpha-trihydroxymethylaminomethane (THAM) salt or PBS. None of these gilts expressed behavioral estrus immediately after PGF2alpha or vehicle treatment. On Day 113, PGF2alpha increased peak relaxin (60 ng/ml) compared with that of controls (34 ng/ml; p < 0.01), whereas progesterone decreased abruptly (4 vs. 16 ng/ml in PGF2alpha and PBS; p < 0.01). Prolactin remained at < 5 ng/ml from Day 98 to 120 in controls but peaked at 33 ng/ml immediately after PGF2alpha treatment on Day 113, and then decreased to levels similar to those of controls on Day 120. Sequential bleeding revealed an acute growth hormone release (4.5 ng/ml) immediately after PGF2alpha injection and return to basal levels (< 0.6 ng/ml) on Days 114-120. PGF2alpha induced abrupt shifts in progesterone, relaxin, prolactin, and growth hormone secretion in hysterectomized gilts that mimicked hormone changes seen in late pregnancy, parturition, and early lactation. These findings provide new insight into the role of PGF2alpha in abruptly changing hormone secretions by aging corpora lutea and the pituitary gland even in the absence of conceptuses or the uterus in the pig.
Asunto(s)
Cuerpo Lúteo/efectos de los fármacos , Cuerpo Lúteo/fisiología , Dinoprost/farmacología , Histerectomía , Porcinos/fisiología , Animales , Cuerpo Lúteo/metabolismo , Estro , Femenino , Hormona del Crecimiento/metabolismo , Cinética , Hipófisis/efectos de los fármacos , Hipófisis/metabolismo , Embarazo , Progesterona/sangre , Progesterona/metabolismo , Relaxina/sangre , Relaxina/metabolismo , Factores de TiempoRESUMEN
Our objective was to examine the influence of feeding and endogenous GH secretion on circulating IGF-I in colostrum-deprived newborn pigs fed colostrum (n = 4), formula (control, n = 4), or water (n = 4). In another four formula-fed pigs, GH was ablated (GRF-A) with two intravenous injections of a GH releasing-factor antagonist (N-Ac-Tyr1,D-Arg2)-GRF(1-29)-NH2. Blood was serially sampled in all pigs to measure plasma IGF-I and GH profiles. Feeding increased plasma IGF-I concentration two- to fourfold and decreased GH secretion. Despite a more than 80% decrease in the plasma GH in GRF-A pigs, the circulating IGF-I concentration was similar to that in control pigs. In colostrum-fed pigs, plasma IGF-I was higher than that in control pigs, despite equal nutrient intake and lower circulating GH. There were no differences in plasma IGF binding protein (IGFBP)-3 levels among the treatment groups. However, the relative abundance of plasma IGFBP-4 was lower, and that of IGFBP-1 higher, in unfed pigs than in any of the three fed groups. The plasma insulin concentration was not different among fed pigs, but it was lower in unfed pigs. Our results indicate that the circulating IGF-I concentration is more dependent on nutrient intake than on GH in newborn pigs, despite relatively high GH concentrations. However, because the nutrient content in the formula was designed to match that of colostrum, a factor other than nutrient intake and GH was responsible for the maximal increase in circulating IGF-I concentration observed in colostrum-fed pigs.
Asunto(s)
Animales Recién Nacidos/metabolismo , Calostro/inmunología , Hormona del Crecimiento/metabolismo , Factor I del Crecimiento Similar a la Insulina/metabolismo , Porcinos/metabolismo , Animales , Animales Recién Nacidos/crecimiento & desarrollo , Animales Recién Nacidos/inmunología , Autorradiografía/veterinaria , Western Blotting/veterinaria , Ligandos , Distribución Aleatoria , Receptores de Neuropéptido/antagonistas & inhibidores , Receptores de Hormona Reguladora de Hormona Hipofisaria/antagonistas & inhibidores , Sermorelina/análogos & derivados , Sermorelina/farmacología , Porcinos/crecimiento & desarrollo , Porcinos/inmunologíaRESUMEN
We evaluated the effects of recombinant porcine somatotropin (pST) and the beta-adrenergic agonist salbutamol on plasam endocrine and metabolite profiles and muscle chemistry in three genotypes of growing barrows (n = 96, 139 d old). Treatments were in a 2 x 2 x 3 factorial arrangement, and main effects were pST (0 or 4 mg/d) and salbutamol (0 or 2.75 ppm); the three genotypes including purebred Meishan (M), 1/4 Duroc, 3/4 White composite (D x Wc), and 1/4 Meishan, 3/4 White composite (M x Wc). Individual pigs were injected daily with buffer or pST at 0700 and allowed ad libitum access to a corn-soybean meal diet (1.2% lysine) and water for 33 d. Plasma was obtained 4 h after injection and 3 h postprandially on d 0, 14, and 28 for determination of growth hormone (GH), insulin, IGF-I, glucose, urea N (PUN), NEFA, triglycerides, and cholesterol. Longissimus and semitendinosus samples were obtained, and protein, RNA, and DNA were quantified and loin chop shear force was measured. In general, plasma hormones and metabolites on d 14 and 28 were not affected by salbutamol in the absence of pST, although salbutamol tended to increase d 14 and 28 GH concentrations. Salbutamol lowered plasma IGF-I (d 14 and 28, P < .05), insulin (d 14, P < .01), and NEFA (d 28, P = .07) when pST was administered, although concentrations still exceeded those for control pigs. Salbutamol reduced (P < .05) IGF-I in M and M x Wc pigs, and GH was not changed in M pigs. Meishan pigs had a greater increase in glucose with pST than M x Wc or D x Wc pigs, although the effect was not consistent over time. Individual treatment with pST caused GH, insulin, IGF-I, glucose, NEFA, and triglycerides to be increased and PUN to be decreased on d 14 and 28. Cholesterol on d 14 and 28 was decreased by pST in M pigs, whereas no effects were found in the other genotypes. Muscle protein and RNA were increased by salbutamol and were consistently lowest for M pigs. Furthermore, pST did not affect muscle protein, but it increased RNA more in M pigs than in the others. Overall, pST and salbutamol seemed to act separately and by different mechanisms to alter muscle composition, but blood criteria generally representing fat metabolism (insulin, glucose, NEFA, triglycerides) were interactively affected. Meishan pigs tended to have greater changes in muscle and plasma composition with pST treatment than did M x Wc or D x Wc pigs.
Asunto(s)
Agonistas Adrenérgicos beta/farmacología , Albuterol/farmacología , Hormona del Crecimiento/farmacología , Músculo Esquelético/efectos de los fármacos , Porcinos/metabolismo , Animales , Análisis Químico de la Sangre/métodos , Análisis Químico de la Sangre/veterinaria , Glucemia/análisis , Glucemia/metabolismo , Composición Corporal/fisiología , Colesterol/sangre , Colesterol/metabolismo , ADN/análisis , ADN/metabolismo , Dieta/veterinaria , Ácidos Grasos no Esterificados/sangre , Ácidos Grasos no Esterificados/metabolismo , Genotipo , Hormona del Crecimiento/sangre , Hormona del Crecimiento/metabolismo , Insulina/sangre , Insulina/metabolismo , Factor I del Crecimiento Similar a la Insulina/análisis , Factor I del Crecimiento Similar a la Insulina/metabolismo , Masculino , Proteínas Musculares/análisis , Proteínas Musculares/genética , Proteínas Musculares/metabolismo , Músculo Esquelético/química , Músculo Esquelético/fisiología , Nitrógeno/sangre , Probabilidad , ARN/análisis , ARN/metabolismo , Radioinmunoensayo/veterinaria , Porcinos/sangre , Porcinos/genética , Triglicéridos/sangre , Triglicéridos/metabolismoRESUMEN
The objective of this study was to determine whether serum concentrations of growth hormone (GH), IGF-I, IGF binding proteins (IGFBP), and glucose at wk 2 and 10 postpartum were associated with the ability of postpartum beef cows to resume cycling when maintained on a limited nutrient environment. Cows (n = 29) were individually fed either 130 or 170 kcal ME x BW-75 x d-1 during nonlactation and 170 or 210 kcal ME x BW-75 x d-1 during lactation for an average of 4.1 yr before sample collection. The proportion of cows that resumed estrus within 20 wk after parturition was less (P < .05) at the lower feeding rate (5 of 14) than at the higher feeding rate (11 of 15). Concentrations of IGF-I increased from wk 2 to 10 in cows that resumed cycling but not in cows that remained anestrous and were less (P < .05) at wk 2 and 10 in cows that remained anestrous compared to cows that resumed cycling. Circulating amounts of IGFBP-2 at wk 2 were greater (P < .05) and IGFBP-3 concentrations were lower (P < .05) in cows that remained anestrous compared to cows that resumed cycling. Cows on the lower feeding rate that did not cycle had lower body condition scores and greater concentrations of GH compared (P < .05) to other cows. At the higher feeding rate, body condition score and concentrations of GH did not differ between cows that did or did not resume cycling. Circulating concentrations of IGF-I and IGFBP-2 and -3 at wk 2 postpartum were indicators of the capacity of energy-restricted cattle to resume cycling after parturition.
Asunto(s)
Bovinos/fisiología , Ingestión de Energía/fisiología , Estro/fisiología , Hormona del Crecimiento/sangre , Proteínas de Unión a Factor de Crecimiento Similar a la Insulina/sangre , Factor I del Crecimiento Similar a la Insulina/análisis , Periodo Posparto/fisiología , Análisis de Varianza , Anestro/fisiología , Animales , Glucemia/análisis , Glucemia/metabolismo , Bovinos/sangre , Femenino , Proteína 2 de Unión a Factor de Crecimiento Similar a la Insulina/sangre , Proteína 3 de Unión a Factor de Crecimiento Similar a la Insulina/sangre , Proteína 4 de Unión a Factor de Crecimiento Similar a la Insulina/sangre , Proteína 5 de Unión a Factor de Crecimiento Similar a la Insulina/sangre , Factor I del Crecimiento Similar a la Insulina/metabolismo , Periodo Posparto/sangre , Pruebas de Precipitina/métodos , Pruebas de Precipitina/veterinaria , Radioinmunoensayo/métodos , Radioinmunoensayo/veterinariaRESUMEN
Onset of puberty (sperm production) occurs at a much younger age (56-84 days) in Meishans than in conventional boars (120-180 days). Throughout postnatal development, Meishans exhibit markedly higher (two- to ten-fold) concentrations of serum FSH, LH and androgens compared with conventional boars, and these high hormone concentrations are maintained at maturity. Increased gonadotrophin concentrations occur only in Meishan males, since concentrations in female Meishans and conventional females do not differ. In the Meishan boar, FSH increases steadily during establishment of sperm production and LH increases markedly after tubule diameter stabilizes, while FSH and LH concentrations are low and change little during this period of rapid testicular growth in conventional boars. The proportion of the testis occupied by Leydig cells is two-fold greater and average Leydig cell size is two- to four-fold larger in Meishans than in conventional boars during the onset of spermatogenesis and into maturity. Testis size in Meishans is only half that of conventional boars at maturity, and the number of Sertoli cells is markedly reduced in Meishans. However, Meishan testes exhibit reduced degeneration of germ cells and spermatids during spermatogenesis, and spermatogenic efficiency per Sertoli cell in Meishans is double that in conventional boars. At maturity, Meishan pituitaries have greater mass, larger gonadotrophs, higher content of FSH, and greater expression of genes for FSH and LH subunits than found in conventional boars. The high concentrations of serum gonadotrophins in the mature Meishan exhibit a typical postcastration rise, respond to GnRH stimulation and are suppressed by gonadal steroids, indicating that the hypothalamo-pituitary-gonadal axis functions normally but with much higher set points in Meishan boars. These unusual endocrine and physiological attributes in boars of the highly prolific Meishan breed offer a unique model for further investigation of factors influencing early onset of puberty and efficiency of male reproductive function.
Asunto(s)
Maduración Sexual/genética , Porcinos/fisiología , Testículo/crecimiento & desarrollo , Animales , Peso Corporal , Hormonas Esteroides Gonadales/sangre , Gonadotropinas Hipofisarias/sangre , Masculino , Tamaño de los Órganos , Porcinos/genética , Porcinos/crecimiento & desarrollo , Testículo/anatomía & histologíaRESUMEN
Previous studies have shown that concentrations of insulin-like growth factor (IGF)-binding proteins (BPs) in bovine follicles before the preovulatory surge of LH are inversely associated with estradiol concentrations, such that decreases in IGFBPs occur with advanced development of antral follicles and atresia is associated with increases in IGFBPs. In the present study, we evaluated serum and follicular fluid at different times after the preovulatory surge of LH to determine whether changes in IGF-I and IGFBPs are associated with the marked changes in the steroidogenic capacity that occur in follicle during this period. Serum and fluid from small follicles (> or = 5-mm diameter) and the preovulatory follicle were collected from cows at the onset of standing estrus or 8.5 and 20.5 h after administration of 100 micrograms GnRH at the onset of estrus (n = 4/time). Concentrations of IGF-I (determined by RIA) did not differ (p > 0.10) among times but were lower (p < 0.05) in fluid from small and preovulatory follicles than those in serum. Profiles of IGFBPs (evaluated by ligand blot analysis) differed among serum and fluid from small and preovulatory follicles. Preovulatory follicles contained IGFBP-3 with little or no other IGFBPs detected. Amount of IGFBP-3 and progesterone did not differ with time of sample collection, but a 13- to 15-fold reduction in estradiol-17 beta and androstenedione occurred between 8.5 and 20.5 h after the LH surge. Small follicles contained IGFBP-2, -3, -4, and -5 (determined by immunoprecipitation). The amount of 28-29-kDa IGFBPs (small form of IGFBP-5 and [or] glycosylated form of IGFBP-4) was inversely associated with concentrations of androstenedione. The 31-kDa form of IGFBP-5 was positively correlated with concentration of progesterone. Serum contained IGFBP-2, -3, and -4, but not IGFBP-5. Circulating IGFBP-2, but not other IGFBPs, decreased by 50% from estrus to 20.5 h after GnRH. In summary, amounts of IGF-I or IGFBPs in preovulatory follicular fluid did not change in response to an LH surge, even though estradiol and androstenedione decreased markedly. The absence of IGFBPs other than IGFBP-3 in bovine preovulatory follicles may allow for increased availability of IGF-I, which is proposed to be important for oocyte maturation and ovulation.
Asunto(s)
Bovinos/metabolismo , Líquido Folicular/metabolismo , Proteínas de Unión a Factor de Crecimiento Similar a la Insulina/metabolismo , Factor I del Crecimiento Similar a la Insulina/metabolismo , Hormona Luteinizante/metabolismo , Ovulación/fisiología , Androstenodiona/metabolismo , Animales , Estradiol/metabolismo , Estro , Femenino , Glicosilación , Hormona Liberadora de Gonadotropina/farmacología , Técnicas de Inmunoadsorción , Proteína 2 de Unión a Factor de Crecimiento Similar a la Insulina/metabolismo , Proteína 3 de Unión a Factor de Crecimiento Similar a la Insulina/metabolismo , Proteína 4 de Unión a Factor de Crecimiento Similar a la Insulina/metabolismo , Proteína 5 de Unión a Factor de Crecimiento Similar a la Insulina/metabolismo , Proteínas de Unión a Factor de Crecimiento Similar a la Insulina/sangre , Folículo Ovárico/metabolismo , Progesterona/metabolismoRESUMEN
We hypothesized that much of the positive effect of porcine somatotropin (pST) on the immune system would be offset by the pST/IGF stimulation of gonadal function and that there would be negative effects on thymic weight and function from increased androgens in males. Male and female hogs (78 boars, 90 gilts) representing three genetic lines (lean, obese, and crossbred meat type) received 0, 2, or 4 mg of recombinant pST/d via implant for 42 d. Blood samples were collected on 0, 7, 14, 28, and 42 d of the trial for changes in pST, IGF-I, IGF-II, thymosin beta 4, dehydroepiandrosterone/dehydroepiandrosterone sulfate (DHEA/DHEASO4), and testosterone concentrations. Thymic, splenic, gonadal, and adrenal weights were collected at slaughter (d 42). Thymic weights increased with dose of pST in both sexes (P < .01), but splenic weights were unaltered. Adrenal weights increased with dose of pST (P < .01), but gonadal weights showed no response to pST in either sex or any line. Overall, concentrations of pST, IGF, and thymosin beta 4 increased with dose of pST. Serum testosterone concentrations in boars declined with dose of pST in lean and obese lines, and DHEA/ DHEASO4 declined in all lines with pST treatment. Testicular testosterone concentrations were not different among lines or doses of pST, indicating no stimulatory effect of increased pST/IGF-I on Leydig cell function. Although pST and its effects through increased circulating IGF are thought to be overall stimulants of growth and protein accretion, the actions on the tissues of the immune system (thymus/spleen) and steroidogenic tissues (adrenal/gonadal) can be selective. Increases in thymic weights and thymosin beta 4 concentrations from pST treatment in boars were partially due to the pST-induced decline in circulatory androgens, and the responses found in this in vivo model do not support pST/IGF effects documented in in vitro systems on Leydig cell function.