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1.
Phys Rev Lett ; 90(4): 041801, 2003 Jan 31.
Artículo en Inglés | MEDLINE | ID: mdl-12570410

RESUMEN

The K2K experiment observes indications of neutrino oscillation: a reduction of nu(mu) flux together with a distortion of the energy spectrum. Fifty-six beam neutrino events are observed in Super-Kamiokande (SK), 250 km from the neutrino production point, with an expectation of 80.1(+6.2)(-5.4). Twenty-nine one ring mu-like events are used to reconstruct the neutrino energy spectrum, which is better matched to the expected spectrum with neutrino oscillation than without. The probability that the observed flux at SK is explained by statistical fluctuation without neutrino oscillation is less than 1%.

2.
Jpn J Vet Res ; 49(2): 115-23, 2001 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-11590919

RESUMEN

Our previous study established protein gene product 9.5 (PGP 9.5), a ubiquitin C-terminal hydrolase, as a specific cytochemical marker of synovial lining cells (type B synoviocytes) in the horse joint. The present study aimed to detect PGP 9.5 in the synovial fluid and shows that PGP 9.5 is a valuable marker of osteoarthritis in the horse. Immunohistochemical staining confirmed rich and consistent localization of PGP 9.5 immunoreactivity in the cytoplasm of synovial lining cells in the normal horse joint. Western blot analysis of synovial fluid from normal joints could detect a significant band corresponding to that contained in the brain and synovial membrane extracts. When 60 synovial fluid samples from normal and abnormal joints were assayed with an enzyme-linked immunosorbent assay (ELISA) system, the concentration of PGP 9.5 tended to be elevated in osteochondrosis dissecance, inflammatory arthropathy and intra-articular fracture, among which a statistically significant elevation was recognizable between the intra-articular fracture and the control. Thus, this study demonstrated the possibility that PGP 9.5, derived from synovial lining cells, may be a new biochemical marker for arthritic disorders of the horse.


Asunto(s)
Enfermedades de los Caballos/metabolismo , Osteoartritis/veterinaria , Líquido Sinovial/metabolismo , Tioléster Hidrolasas/metabolismo , Animales , Western Blotting/veterinaria , Ensayo de Inmunoadsorción Enzimática/veterinaria , Femenino , Caballos , Inmunohistoquímica/veterinaria , Osteoartritis/diagnóstico , Ubiquitina Tiolesterasa
3.
Neurosci Res ; 39(4): 463-8, 2001 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-11274745

RESUMEN

Veratridine is a neurotoxin that induces persistent activation of sodium channels in excitable cells. We investigated the effects of this toxin on excitatory synaptic transmission in CA3 neurons of juvenile rat hippocampus using whole-cell patch-clamp and field-potential recordings. The population spikes evoked by electrical stimulation of the mossy fiber were gradually enhanced after washout of veratridine (0.3 microM), but they were not enhanced by the co-application of veratridine and an N-methyl-D-aspartate (NMDA) receptor antagonist (D-APV, 30 microM). When a pipette solution contained QX-314 that antagonized the effect of veratridine in the recorded neuron, oscillatory membrane depolarization appeared in the early stage during bath-application of veratridine and gradually decreased in the late stage. After washout of veratridine, however, the oscillatory depolarization was gradually restored and maintained for at least 3 h. This oscillatory depolarization was also abolished by D-APV. We suggest that the activation of NMDA receptors is involved in the veratridine-induced long-lasting enhancement in the excitatory synaptic transmission in rat CA3 hippocampal neurons.


Asunto(s)
Hipocampo/efectos de los fármacos , Potenciación a Largo Plazo/efectos de los fármacos , Neurotoxinas/farmacología , Células Piramidales/efectos de los fármacos , Transmisión Sináptica/efectos de los fármacos , Veratridina/farmacología , 2-Amino-5-fosfonovalerato/farmacología , 6-Ciano 7-nitroquinoxalina 2,3-diona/farmacología , Animales , Relojes Biológicos/efectos de los fármacos , Relojes Biológicos/fisiología , Membrana Celular/efectos de los fármacos , Membrana Celular/metabolismo , Estimulación Eléctrica , Potenciales Evocados/efectos de los fármacos , Potenciales Evocados/fisiología , Antagonistas de Aminoácidos Excitadores/farmacología , Hipocampo/citología , Hipocampo/metabolismo , Potenciación a Largo Plazo/fisiología , Potenciales de la Membrana/efectos de los fármacos , Potenciales de la Membrana/fisiología , Fibras Musgosas del Hipocampo/efectos de los fármacos , Fibras Musgosas del Hipocampo/metabolismo , Técnicas de Cultivo de Órganos , Células Piramidales/citología , Células Piramidales/metabolismo , Ratas , Ratas Wistar , Receptores AMPA/efectos de los fármacos , Receptores AMPA/metabolismo , Receptores de N-Metil-D-Aspartato/antagonistas & inhibidores , Receptores de N-Metil-D-Aspartato/metabolismo , Canales de Sodio/efectos de los fármacos , Canales de Sodio/metabolismo , Transmisión Sináptica/fisiología
4.
Br J Radiol ; 72(863): 1046-51, 1999 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-10700819

RESUMEN

The purpose of the study was to assess the signal intensities of arachnoid granulations within the dural sinuses using the FLAIR sequence for differentiation of space-occupying lesions in and adjacent to the dural sinuses. We retrospectively reviewed MR images of the brain of 1118 consecutive subjects, ranging in age from 0 to 93 years (mean 57.2 years). Nodules within the dural sinuses with signal intensities similar to that of cerebrospinal fluid (CSF) on both T1 and T2 weighted images were defined as arachnoid granulations. The location, signal intensity on T1 weighted spin echo (SE), T2 weighted fast SE and FLAIR images, the impression on the inner table of the skull, and the size of the lesion were assessed. 112 subjects (10.0%), age range 4-89 years old (mean 58.9 years), were found to have 134 arachnoid granulations. The commonest location was the transverse sinus, with 115 granulations (85.8%). The prevalence of the granulations showed a peak in the sixth decade of age. All granulations were isointense relative to CSF on T2 weighted images and almost all lesions were isointense relative to CSF on T1 weighted images. On FLAIR images, 90.3% of the granulations were isointense relative to CSF and the other 9.7% granulations were slightly hyperintense compared with the CSF. 21 (15.7%) subjects showed impressions on the inner table; one case involved the outer table. In conclusion, arachnoid granulations were isointense or slightly hyperintense relative to CSF on FLAIR. FLAIR images are helpful in differentiating arachnoid granulations from other dural sinus lesions or skull lesions which have an intensity similar to that of CSF on T1 weighted and T2 weighted images.


Asunto(s)
Venas Cerebrales/anatomía & histología , Tejido de Granulación/anatomía & histología , Angiografía por Resonancia Magnética/métodos , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Aracnoides/anatomía & histología , Niño , Preescolar , Duramadre/anatomía & histología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Flujo Pulsátil , Estudios Retrospectivos , Espacio Subaracnoideo/anatomía & histología
6.
Nihon Kyobu Geka Gakkai Zasshi ; 39(12): 2140-4, 1991 Dec.
Artículo en Japonés | MEDLINE | ID: mdl-1774500

RESUMEN

The efficacy and method of administration of recombinant human erythropoietin (EPO) in adult cardiac surgical patients when given preoperatively was evaluated. We used EPO intravenously (iv) with 40 mg ferric oxide for a total of consecutive 47 patients. The patients were divided into group A (n = 14; EPO 200 IU/kg iv 3 times a week from 3 weeks prior to surgery to 2 weeks after surgery, donation of 800 ml) and group B (n = 33; EPO 200 IU/kg iv everyday from 8 days prior to surgery to 2 weeks after surgery, donation of 400 ml). Control groups were group AO (n = 11; donation of 835 +/- 33 ml from 14.8 days prior to surgery) and group BO (n = 7; donation of 406 +/- 34 ml at 7.3 days prior to surgery). All the EPO-treated patients received no homologous blood transfusion while 2 of patients in group BO received some homologous blood transfusion. A hemoglobin change between pre-donation and surgery was +0.14 +/- 1.3 (g/dl) in group A, +0.04 +/- 1.0 (g/dl) in group B, -1.7 +/- 1.3 (g/dl) in group AO and -1.0 +/- 0.6 (g/dl) in group BO. In a comparison of post-surgical hemoglobin levels between group A and group B, we demonstrated that the level in group B, +2.1 +/- 1.8 (g/dl) was significantly higher than that in group A, +11.1 +/- 1.6 (g/dl) 2 weeks after surgery. There was no evidence to show an aggravation of anemia in the pre-surgical period in EPO-treated groups.(ABSTRACT TRUNCATED AT 250 WORDS)


Asunto(s)
Anemia/terapia , Transfusión de Sangre Autóloga , Procedimientos Quirúrgicos Cardíacos , Eritropoyetina/uso terapéutico , Complicaciones Posoperatorias/terapia , Humanos , Proteínas Recombinantes/uso terapéutico
15.
J Virol Methods ; 3(5): 293-301, 1981 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-6277975

RESUMEN

The envelope glycoproteins of influenza virus (HA and NA) and paramyxovirus (HN and F) were visualized on the surface of infected cells by immunoelectron microscopy using the indirect immunoperoxidase technique. In X7 influenza virus-infected fibroblasts, the hemagglutinin (HA) and the neuraminidase (NA) were observed on the cell membrane respectively 2 and 3--4 h after infection. The antigens were initially seen as discrete patches and later evenly distributed along the plasma membrane prior to budding. Antibody induction of HA and NA was observed as cytoplasmic inclusions, with peroxidase-positive activity, attributed to endocytosis. The redistribution of HA and NA supports the hypothesis of lateral mobility of the viral glycoproteins in cellular membranes as visualized by the immunoperoxidase method. The glycoproteins of Sendai virus, in infected Madin--Darby bovine kidney cells, were found to be evenly distributed along the plasma membrane and endoplasmic reticulum, the latter by the indirect microperoxidase method. The immunoperoxidase methods may be useful for investigating the polarized distribution of envelope glycoproteins.


Asunto(s)
Glicoproteínas/análisis , Virus de la Influenza A/ultraestructura , Virus de la Parainfluenza 1 Humana/ultraestructura , Proteínas Virales/análisis , Animales , Bovinos , Línea Celular , Membrana Celular/ultraestructura , Embrión de Pollo , Glicoproteínas Hemaglutininas del Virus de la Influenza , Hemaglutininas Virales/análisis , Técnicas para Inmunoenzimas , Riñón , Microscopía Electrónica , Neuraminidasa/análisis , Proteínas del Envoltorio Viral
16.
Endocrinol Jpn ; 22(2): 175-80, 1975 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-168060

RESUMEN

We have presented here a case of atypical insulinoma. Despite the recurrent episodes of hypoglycemic symptoms, the plasma level of insulin has never been excessive at fasting or by regular provocative tests. Detailed examination had demonstrated qualitative abnormality of insulin secretion. Hyposuppressibility of insulin secretion by hypoglycemia, borderline diabetic curve of glucose tolerance test, blunted response ot insulin to glucagon and leucine were the principle characteristics of these abnormalities. After removal of adenoma, insulin response to glucose, glucagon and leucine was improved. Only secretion provoked a high level of insulin and this abnormal elevation was no longer seen after the removal of adenoma. A removed elevation was no longer seen after the removal of adenoma. A removed insulinoma contained 25 U of immunoreactive insulin per gram tissue, but was negative for aldehyde-fuchsin staining. On electromicroscopy only atypical beta-cell granules were seen.


Asunto(s)
Adenoma de Células de los Islotes Pancreáticos/fisiopatología , Insulina/metabolismo , Neoplasias Pancreáticas/fisiopatología , Adenoma/patología , Adenoma/cirugía , Adenoma de Células de los Islotes Pancreáticos/cirugía , Glucemia/metabolismo , Femenino , Glucagón , Prueba de Tolerancia a la Glucosa , Humanos , Hipoglucemia/metabolismo , Insulina/sangre , Secreción de Insulina , Leucina , Microscopía Electrónica , Persona de Mediana Edad , Neoplasias Pancreáticas/patología , Neoplasias Pancreáticas/cirugía
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