RESUMEN
The aim of the study was to modify a simple and widely used spectrophotometric assay for MAO activity evaluation with 2,4-dinitrophenylhydrazine. A modified procedure includes molar absorption coefficients of 2,4-DNP-hydrazone benzaldehyde and 2,4-DNP-hydrazone 5-hydroxyindolylacetaldehyde as 2.3 × 104mol-1l cm-1 and 1.0 × 104 mol-1l cm-1, respectively. Such an approach allows to express specific enzyme activity as nmol product formed/min/mg protein.
Asunto(s)
Inhibidores de la Monoaminooxidasa/química , Monoaminooxidasa/metabolismo , Fenilhidrazinas/química , Acetaldehído/química , Benzaldehídos/química , Activación Enzimática , Hidrazonas/química , Inhibidores de la Monoaminooxidasa/metabolismo , Fenilhidrazinas/metabolismo , Unión Proteica , EspectrofotometríaRESUMEN
The effects of fullerenol C60(OH)24 in doses of 0.1-100 µg/ml on erythropoiesis were studied in the culture of erythroblastic islets of the bone marrow. Fullerenol in concentrations of 10 and 100 µg/ml had negative effects on the development of erythroid tissue: it inhibits proliferation of erythroid cells, delays erythroblast maturation, decelerates recruitment of erythroid CFU to differentiation, and suppresses repeated involvement of macrophages in erythroblastic islets.
Asunto(s)
Eritroblastos/efectos de los fármacos , Células Eritroides/efectos de los fármacos , Eritropoyesis/efectos de los fármacos , Fulerenos/farmacología , Macrófagos/efectos de los fármacos , Animales , Animales no Consanguíneos , Recuento de Células Sanguíneas , Diferenciación Celular/efectos de los fármacos , Células Cultivadas , Eritroblastos/citología , Células Eritroides/citología , Macrófagos/citología , Masculino , RatasRESUMEN
Reactive anxiety, immune system function, and metabolism were studied in healthy volunteers. Temperament characteristics, state of health, number of leukocytes and platelets, and concentration of glucose, albumin, and fibrinogen significantly differed in subjects with low, moderate, and high level of reactive anxiety.
Asunto(s)
Trastornos de Adaptación/inmunología , Ansiedad/inmunología , Inmunidad/inmunología , Adulto , Femenino , Estado de Salud , Humanos , Leucocitos , Masculino , Persona de Mediana Edad , Fagocitosis/inmunología , Albúmina Sérica , Seroglobulinas , Estadísticas no Paramétricas , Encuestas y Cuestionarios , TemperamentoRESUMEN
Drinking of mineral waters influences human immune system. Among the mechanisms of this action is a stress effect confirmed by increasing levels of glucocorticoids, catecholamines, by hyperglycemia, etc. Regular drinking of mineral water brings about adaptation. Development of long-term adaptation provokes defensive cross effects. It is suggested that an immunotropic action of mineral waters may be a manifestation of a defensive cross effect of the organism adaptation. Immunostimulation may also be related to suppression of T-suppressors activity and changes in cytokines levels.
Asunto(s)
Sistema Inmunológico , Aguas Minerales , Citocinas/análisis , Humanos , Linfocitos T Reguladores/inmunologíaRESUMEN
The investigation used the mineral water (MW) Uralochka obtained from well 4638 located on the territory of the sanatorium Ural (the village of Khomutino, Chelyabinsk Region). Experimental findings suggest that the use of the Uralochka water for 14 days lowers experimental animals' sensitivity to stress. This is manifested by that MW use virtually completely eliminated the hematological manifestations of stress, poststress depression of a cell immunity response and prevented the reduction of cell counts in the abdominal cavity. The immunostimulating effect of the Uralochka MW on humoral immunity and the phagocytosis of peritoneal macrophages preserved with the combined influence of stress and MW.
Asunto(s)
Aguas Minerales , Bicarbonato de Sodio , Cloruro de Sodio , Estrés Psicológico/sangre , Estrés Psicológico/inmunología , Animales , Formación de Anticuerpos , Hipersensibilidad Tardía , Inmunidad Celular , Recuento de Leucocitos , Macrófagos Peritoneales/patología , Masculino , Ratas , Ratas WistarRESUMEN
Mineral water Uralochka tried in rats is obtained from the spring in the territory of the sanatorium Ural (Chelyabinsk Province). The mineral water contains chloride, hydrocarbonate ions and sodium cations, mineralization is 4.13 g/dm3. A 15-day course of Uralochka drinking did not influence much reaction of delayed hypersensitivity, but increased the absolute number of antibody-forming cells by 95.86%, raised the ability of peritoneal macrophages to absorb latex particles. Thus, mineral water Uralochka can raise intensity of humoral immune response and the ability of macrophages for phagocytosis of the latex particles. Phagocytosis activity and intensity increased by 48.27 and 62.13%, respectively.
Asunto(s)
Sistema Inmunológico/efectos de los fármacos , Aguas Minerales , Bicarbonato de Sodio/farmacología , Cloruro de Sodio/farmacología , Animales , Formación de Anticuerpos/efectos de los fármacos , Sistema Inmunológico/inmunología , Inmunidad Celular/efectos de los fármacos , Masculino , Ratas , Ratas Wistar , Siberia , Factores de TiempoRESUMEN
Male Wistar rats exposed to different stresses developed shifts in the brain and liver monoaminoxidase activity. In the so called "cognitive" stimulation, the activity was enhanced in the brain and reduced in liver. Mild stresses also enhanced the activity in the brain. Extreme stimulation (starch peritonitis) caused a significant diminishing of the activity in the brain. All the stress schedules accompanied by enhancement of the brain monoaminoxidase activity increased the rats' tolerance of acute hypoxic hypoxia. Negative correlations between the blood lactic acid contents and the brain monoaminoxidase activity were revealed in rats of both the control and the "cognitive" groups. The findings suggest a direct interrelationship between post-stress shifts of the brain monoaminoxidase activity and the hypoxia tolerance.
Asunto(s)
Hipoxia/etiología , Monoaminooxidasa/metabolismo , Estrés Fisiológico/enzimología , Enfermedad Aguda , Animales , Encéfalo/enzimología , Susceptibilidad a Enfermedades , Hipoxia/metabolismo , Inmovilización , Hígado/enzimología , Masculino , Peritonitis/complicaciones , Ratas , Ratas Wistar , Estrés Fisiológico/complicacionesRESUMEN
After both thermal and mechanical injuries, mice are shown to have depressed functions of phagocytes (primarily neutrophils), suppressed ability of an immune response to antigens and decreased skin inflammatory and reparative activities. It is suggested that a neutrophilic secretory defect and a decrease in the production of stimulating factors might be a cause of the above effects. After thermal and mechanical injuries, injection of neutrophilokin extracted from latex-activated neutrophilic supernatant into the mice promoted recovered phagocytic functional activity, higher humoral and cellular immune response and increased the rate of standard burn wound healing. Neutrophilokin also accelerated clavus formation in mice with mechanical injury. Thus, it can be concluded that neutrophils and their low-molecular secretory products play an important role in regulating immune and inflammatory reparative homeostasis in thermal and mechanical injuries.
Asunto(s)
Curación de Fractura/inmunología , Inmunidad Celular/fisiología , Neutrófilos/fisiología , Cicatrización de Heridas/inmunología , Animales , Quemaduras/inmunología , Modelos Animales de Enfermedad , Fracturas Óseas/inmunología , Miembro Posterior/lesiones , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Fagocitosis/inmunologíaRESUMEN
Experimental data have shown that repeated exposure to both "cognitive" (recognized by the nervous system) and "noncognitive" (recognized by the immune system) stimuli induces so-called "tolerant (resource-preserving adaptation strategy". This strategy is characterized by enhanced hypoxia tolerance, reduced sensitivity to stress (catabolic) hormones, such as glucocorticoids and adrenomimetics, and increased sensitivity to insulin (central anabolic hormone). Such shifts appear more pronounced in case of "noncognitive" stimulation, which is also capable of inducing a hypocatabolic state. Nonspecific immunostimulation associated with repeated stress is considered as an appreciable stress-limiting process despite initial "cognitivity" of stressogenic stimulation.
Asunto(s)
Adaptación Fisiológica , Conducta de Elección/fisiología , Cognición/fisiología , Sistema Inmunológico/fisiología , Estrés Psicológico/fisiopatología , Adaptación Fisiológica/efectos de los fármacos , Animales , Conducta de Elección/efectos de los fármacos , Cognición/efectos de los fármacos , Citocinas/farmacología , Sistema Inmunológico/efectos de los fármacos , Tolerancia Inmunológica/efectos de los fármacos , Tolerancia Inmunológica/fisiología , Neuroinmunomodulación/efectos de los fármacos , Neuroinmunomodulación/fisiología , Neutrófilos , Ratas , Ratas Wistar , Restricción FísicaAsunto(s)
Bronquitis/metabolismo , Peroxidación de Lípido , Pulmón/metabolismo , Isquemia Miocárdica/metabolismo , Neutrófilos/metabolismo , Adulto , Bronquios/metabolismo , Bronquitis/sangre , Bronquitis/complicaciones , Enfermedad Crónica , Humanos , Masculino , Persona de Mediana Edad , Isquemia Miocárdica/sangre , Isquemia Miocárdica/complicaciones , Oxígeno/metabolismo , Alveolos Pulmonares/metabolismoAsunto(s)
Hemodinámica/fisiología , Isatina , Inhibidores de la Monoaminooxidasa/orina , Personalidad/fisiología , Adulto , Ansiedad/fisiopatología , Ansiedad/psicología , Ansiedad/orina , Biomarcadores/orina , Femenino , Humanos , Inteligencia/fisiología , Masculino , Estrés Psicológico/fisiopatología , Estrés Psicológico/psicología , Estrés Psicológico/orinaRESUMEN
Experimental data are provided which substantiate the concept on reciprocal changes in immune reactivity and stress sensitivity as a basis for selection of adaptation strategy under unfavorable conditions. The data obtained suggest that transition from the resistant (stress) strategy of adaptation to hypobiosis is a prerequisite for maintenance of the immune reactivity under extreme conditions. Stimulation of the immune system per se may provide for transition from stress to the tolerant (hypobiotic) strategy of adaptation.
Asunto(s)
Adaptación Fisiológica/inmunología , Células Productoras de Anticuerpos/inmunología , Estrés Fisiológico/inmunología , Proteínas de Fase Aguda/inmunología , Adaptación Fisiológica/efectos de los fármacos , Adyuvantes Inmunológicos/farmacología , Antagonistas Adrenérgicos beta/farmacología , Animales , Células Productoras de Anticuerpos/efectos de los fármacos , Encéfalo/enzimología , Encéfalo/inmunología , Ácidos Grasos no Esterificados/sangre , Glucógeno/metabolismo , Hipersensibilidad Tardía/inmunología , Hipoxia/inmunología , Inmovilización , Interleucina-1/sangre , Interleucina-1/inmunología , Macrófagos Peritoneales/efectos de los fármacos , Macrófagos Peritoneales/inmunología , Monoaminooxidasa/metabolismo , Actividad Motora/efectos de los fármacos , Actividad Motora/inmunología , Neutrófilos/efectos de los fármacos , Neutrófilos/inmunología , Propranolol/farmacología , Ratas , Ratas Wistar , Estrés Fisiológico/sangre , Estrés Fisiológico/metabolismo , Estrés Fisiológico/fisiopatologíaRESUMEN
AIM: Comparison of the levels of lipid peroxidation (LPO) products in condensate of the exhaled air (CEA) and in the biopsy samples from the inflammation focus. MATERIALS AND METHODS: Extraction spectrophotometry was used to measure LPO products in CEA and biopsies from 30 males aged 30-60 years suffering from chronic bronchitis and 30 healthy controls of the same age. RESULTS: There was activation of local accumulation of isopropanol-soluble LPO products in the bronchopulmonary system accompanied by lowered content of lipoperoxides and high antioxidant activity in CEA. CONCLUSION: Chronic bronchitis is characterized by multidirectional shifts in LPO in the inflammation focus and CEA.
Asunto(s)
Bronquitis/metabolismo , Peroxidación de Lípido , Adulto , Biopsia , Pruebas Respiratorias , Bronquios/química , Bronquios/patología , Bronquitis/patología , Enfermedad Crónica , Radicales Libres/análisis , Humanos , Masculino , Persona de Mediana Edad , EspectrofotometríaRESUMEN
Local inflammatory process is associated with enhancement of blood sera antioxidative activity (BSAA). The latter phenomenon was demonstrated in rats exposed to burn injury and in man with chronic prostatitis (HP). BSAA increment seems to be intended for limiting of necrosogenic process in inflammatory focus. Adaptive character of BSAA increase is illustrated by results of separate experimental series which proved antiinflammatory and antihypoxic activity of central blood plasma antioxidant-caeruloplasmin.
Asunto(s)
Reacción de Fase Aguda/sangre , Antioxidantes/metabolismo , Quemaduras/sangre , Ceruloplasmina/metabolismo , Prostatitis/sangre , Animales , Tamaño de la Célula , Enfermedad Crónica , Humanos , Hígado/patología , Masculino , Ratones , Ratones Endogámicos C57BL , Peritonitis/sangre , Peritonitis/inducido químicamente , RatasAsunto(s)
Glucemia/fisiología , Diabetes Mellitus Experimental/prevención & control , Estrés Psicológico/sangre , Animales , Glucemia/metabolismo , Colesterol/sangre , Diabetes Mellitus Experimental/psicología , Hidrocortisona/sangre , Hipoglucemiantes/farmacología , Inmovilización , Insulina/sangre , Insulina/farmacología , Resistencia a la Insulina/fisiología , Masculino , Ratas , Estrés Psicológico/fisiopatología , Triglicéridos/sangreRESUMEN
Neutrophilokine, a latex-activated neutrophilic secretory product, was tested for effects on the body's response to immobilization stress. Preadministration of neutrophilokine decreased the magnitude of post-stress changes: neutrophilic leukocytosis, lymphopenia, and stress-induced ulcerogenesis in the gastric mucosa. It is concluded that triple administration of neutrophilokine induces adaptation to immobilization stress.