RESUMEN
Cells of various organs and systems perform their functions and intercellular interactions not in an inert environment, but in the microenvironment of tissue fluids. Violations of the normal drainage of tissue fluids accompany lymphedema. An important mechanism of angiogenesis and vasculogenesis regulation in tissue fluids is the production and reception of vascular endothelial growth factors in combination with the regulation of matrix metalloproteinases. The aim of the work was to perform: a comparative analysis of some polymorphisms of vascular endothelial growth factor and their receptors and the genes encoding matrix metalloproteinases in two forms of lymphedema; an analysis of the relationship of these genes' polymorphisms with the levels of vascular endothelial growth factor and matrix metalloproteinases and their inhibitors in serum and affected tissues. Polymorphism of VEGF (rs699947, rs3025039), KDR (rs10020464, rs11133360), NRP2 (rs849530, rs849563, rs16837641), matrix metalloproteinases MMP2 (rs2438650), MMP3 (rs3025058), MMP9 (rs3918242), Timp1 (rs6609533) and their combinations were analyzed by the Restriction Fragment Length Polymorphism method and TaqMan RT-PCR. The serum and tissue fluid levels were determined using the ELISA test system. Changes in the frequency distribution of MMP2 genotypes in primary and MMP3 in secondary lymphedema are shown. Significant frequency differences in NRP2 genotypes were revealed by comparing primary and secondary lymphedema. Features of the distribution of complex genotypes in primary and secondary lymphedema were revealed. The correlation analysis revealed the interdependence of the concentrations of the MMP, TIMP and VEGF products and differences in the structure of the correlation matrices of patients with both forms of lymphedema. It was shown that, in primary lymphedema, genotypes associated with low MMP2 and TIMP2 in serum and tissue fluid are detected, while in secondary lymphedema, other associations of the production levels with combined genetic traits are observed.
RESUMEN
Immunohistochemical detection of the LYVE-1 marker in healthy human full-thickness skin (the epidermis and the dermis) was carried out. LYVE-1 expression was found in the endothelium of lymphatic capillaries located in the papillary dermis, in the endothelium of larger lymphatic vessels of the reticular dermis, and in fibroblasts, which indicates their joint participation in hyaluronan metabolism. LYVE-1+ staining detected for the first time in cells of the stratum basale, the stratum spinosum, and the stratum granulosum of healthy human epidermis indicates their participation in hyaluronan metabolism and allows us to consider the spaces between epidermis cells as prelimphatics.
Asunto(s)
Epidermis , Ácido Hialurónico , Vasos Linfáticos , Piel , Proteínas de Transporte Vesicular , Humanos , Ácido Hialurónico/metabolismo , Proteínas de Transporte Vesicular/metabolismo , Proteínas de Transporte Vesicular/genética , Piel/metabolismo , Vasos Linfáticos/metabolismo , Epidermis/metabolismo , Ligandos , Fibroblastos/metabolismo , Dermis/metabolismo , Sistema Linfático/metabolismo , Adulto , Femenino , Masculino , InmunohistoquímicaRESUMEN
Polymorphism of genes of transforming growth factor TGFB and its receptors (TGFBRI, TGFBRII, and TGFBRIIII) in patients with primary open-angle glaucoma was analyzed. The frequency of the TGFBRII CC genotype in patients is increased relative to the control group (OR=6.10, p=0.0028). Heterozygosity in this polymorphic position is reduced (OR=0.18, p=0.0052). As the effects of TGF-ß is mediated through its receptors, we analyzed complex of polymorphic variants of the studied loci in the genome of patients. Two protective complexes consisting only of receptor genes were identified: TGFBRI TT:TGFBRII CG (OR=0.10, p=0.02) and TGFBRII CG:TGFBRIII CG (OR=0.09, p=0.01). The study showed an association of TGFBRII polymorphism with primary open-angle glaucoma and the need to study functionally related genes in the development of the disease, which should contribute to its early diagnosis and prevention.
Asunto(s)
Glaucoma de Ángulo Abierto , Humanos , Glaucoma de Ángulo Abierto/genética , Femenino , Masculino , Persona de Mediana Edad , Siberia , Anciano , Polimorfismo de Nucleótido Simple/genética , Predisposición Genética a la Enfermedad/genética , Receptores de Factores de Crecimiento Transformadores beta/genética , Frecuencia de los Genes/genética , Receptor Tipo II de Factor de Crecimiento Transformador beta/genética , Estudios de Casos y Controles , Genotipo , Factor de Crecimiento Transformador beta/genética , Receptor Tipo I de Factor de Crecimiento Transformador beta/genética , Polimorfismo Genético/genéticaRESUMEN
We studied the relationship between the level of cytokines in the lymph of the thoracic duct and the morphometric parameters of the mesenteric lymph nodes after surgical treatment of breast cancer, chemotherapy, and administration of fragmented (double-stranded, dsDNA) human DNA. In comparison with surgical treatment and with chemotherapy alone, administration of a human dsDNA has a stimulating effect on the T-cell link of the immune response. In the paracortical zone, the relationship between the chemokine MCP-1 and increased content of small lymphocytes in this zone was revealed. Interrelations of IL-2 cytokines with small lymphocytes and of IL-4 with medium lymphocytes were revealed in germinal centers. We also observed interrelations of IL-7 with small lymphocytes and IL-4 with macrophages in the medullary cords, chemokine MIP-1α with immature and mature plasma cells (the number of these cells is reduced), and of MCP-1 with immunoblasts (the number of which is also reduced) in the medullary sinuses.
Asunto(s)
Adyuvantes Inmunológicos , Neoplasias de la Mama , Citocinas , ADN , Ganglios Linfáticos , Neoplasias Mamarias Animales , Femenino , Neoplasias de la Mama/tratamiento farmacológico , Neoplasias de la Mama/cirugía , Neoplasias de la Mama/terapia , Quimiocinas/metabolismo , Citocinas/metabolismo , ADN/administración & dosificación , Interleucina-4/metabolismo , Linfa/metabolismo , Ganglios Linfáticos/metabolismo , Animales , Ratas , Ratas Wistar , Neoplasias Mamarias Animales/tratamiento farmacológico , Neoplasias Mamarias Animales/cirugía , Neoplasias Mamarias Animales/terapia , Adyuvantes Inmunológicos/administración & dosificación , Linfocitos T/inmunologíaRESUMEN
The expression of microRNA (miR-21, miR-221, miR-27a, and miR-429) was studied normal and tumor breast tissues of female Wistar rats before and after photodynamic therapy. In breast cancer, the levels of oncogenic microRNA (miR-21, miR-221, and miR-27a) were increased, while the level of tumor-suppressing miR-429 was reduced in comparison with the intact group. After photodynamic therapy, suppression of the expression levels of oncogenic microRNAs (miR-21, miR-221, and miR-27a) was noted. The level of tumor-suppressing miR-429 in breast tumor tissues remained reduced, as in the untreated breast cancer group.
Asunto(s)
MicroARNs , Neoplasias , Fotoquimioterapia , Animales , Línea Celular Tumoral , Proliferación Celular , Femenino , Regulación Neoplásica de la Expresión Génica , MicroARNs/genética , MicroARNs/metabolismo , Neoplasias/genética , Ratas , Ratas WistarRESUMEN
Induced inflammation of reproductive organs in female Wistar rats was associated with an increase in the diameters of arteries and veins and number of blood vessels in the ovary medulla in combination with an increase in the number of lymphatic vessels; these changes were accompanied by reduction of the ovarian reserve and number of yellow bodies. Intravenous and submucosal injection of bone marrow multipotent mesenchymal stromal cells (BÐ-ÐÐSC) led to further increase in the diameters of arteries and veins and number of primordial and primary follicles. The injection of conditioned medium of BÐ-ÐÐSC cultures generally produced the same effects, which could demonstrate the secretory mechanisms of their influence on local angiogenesis and folliculogenesis.
Asunto(s)
Médula Ósea , Células Madre Mesenquimatosas , Animales , Medios de Cultivo Condicionados/farmacología , Femenino , Genitales , Inflamación , Metionina/análogos & derivados , Ratas , Ratas Wistar , Compuestos de SulfonioRESUMEN
We studied interrelationships between the cytoarchitectonics of the mesenteric lymph node and the levels of microRNA-21, microRNA-221/222, and microRNA-429 in the lymph, blood serum, and breast tissues in female Wistar rats with chemically induced breast cancer. After polychemotherapy, significant correlations were found between miRNA-221 and the number of lymphoblasts in the germinal centers and between miRNA-222 and the number of lymphoblasts in the germinal centers and macrophages in the medullary cords of the mesenteric lymph nodes.
Asunto(s)
MicroARNs , Neoplasias , Animales , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Femenino , Ganglios Linfáticos/patología , MicroARNs/genética , Neoplasias/patología , Ratas , Ratas WistarRESUMEN
We performed a correlation analysis of the morphometry of mesenteric lymph nodes and cytokines of the thoracic duct lymph in chemically induced breast cancer (BC) and after surgical treatment of BC and neoadjuvant chemotherapy for BC according to the CMF scheme. After surgical treatment, correlations were found between the level of cytokines in the thoracic duct lymph and morphological changes in the mesenteric lymph nodes. The observed correlations can be a result of total resection of the tumor and increased immunomodulatory and antitumor action of cytokines. Comparison of the effect of chemotherapy and surgical treatment revealed correlations between cytokine concentrations in the lymph and structural changes in the lymph nodes, which can be related to weakening of local immunity and antitumor effect of cytokines.
Asunto(s)
Neoplasias de la Mama , Terapia Neoadyuvante , Neoplasias de la Mama/tratamiento farmacológico , Neoplasias de la Mama/patología , Neoplasias de la Mama/cirugía , Citocinas/farmacología , Femenino , Humanos , Ganglios Linfáticos/patología , Metástasis Linfática/patologíaRESUMEN
We performed a morphometric analysis of mesenteric lymph nodes in rats with breast cancer induced by administration of N-methyl-N-nitrosourea. The volume of the paracortical zone and the number of mature plasma cells in the medullary sinuses were increased and the volume of lymphoid nodules with germinal centers and the number of macrophages were decreased in the group with tumor resection and chemotherapy in comparison with untreated rats with breast cancer. In rats receiving fragmented human double-stranded DNA in combination with adjuvant therapy, the volume of marginal and medullary sinuses and the number of small lymphocytes and macrophages in the paracortical zone increased in comparison with the group receiving chemotherapy without DNA preparation; the volume of lymphoid nodules with germinal centers returned to the level observed in the intact group; the volume of medullary substance and proliferative activity of cells in the germinal centers and medullary substance decreased, the number of mature plasma cells returned to normal in the medullary substance and decreased in the medullary sinuses.
Asunto(s)
ADN/uso terapéutico , Ganglios Linfáticos/patología , Neoplasias Mamarias Experimentales/terapia , Animales , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Terapia Combinada , Ciclofosfamida/administración & dosificación , ADN/química , Fragmentación del ADN , Femenino , Fluorouracilo/administración & dosificación , Humanos , Metástasis Linfática , Neoplasias Mamarias Experimentales/inducido químicamente , Neoplasias Mamarias Experimentales/patología , Mastectomía , Mesenterio , Metotrexato/administración & dosificación , Metilnitrosourea , Ratas , Ratas Wistar , Resultado del TratamientoRESUMEN
We studied the levels of serum and lymph cytokines involved in the pathogenesis of BC. BC was induced by injection of N-methyl-N-nitrosourea to Wistar rats. The animals underwent surgery, or received polychemotherapy (cyclophosphamide, methotrexate, and 5-fluorouracil), or surgical treatment was combined with polychemotherapy; in a special group, Panagen (fragmented DNA) was added to polychemotherapy. Cytokine concentration in the lymph was measured using Bio-Plex Pro Rat Cytokoness 24-Plex Assay test system (Bio-Rad). In rats with BC, the content of most studied cytokines (IL-1ß, IL-2, IL-4, IL-6, IL-7, IL-12, IL-13, MIP-1α, MIP-3α, RANTES, TNFα, and MCP-1) in the lymph and blood was significantly higher, while the content of IL-10 and GRO/KC was lower than in intact animals. Surgical resection of the tumor led to a significant decrease in the content of both pro- and anti-inflammatory cytokines in the lymph. Polychemotherapy led to a significant decrease in the content of IL-1ß, IL-4, IL-6, IL-7, MIP-1α, MIP-3α, and RANTES in the serum and lymph. Comparison of the cytokine content in the serum and lymph of operated animals after polychemotherapy with and without Panagen showed that the content of most cytokines (IL-5, IL-6, IL-7, IL-10, IL-13, IL-17A , IL-18, GRO/KC, IFNγ, and MIP-3α) was higher after Panagen administration.
Asunto(s)
Biomarcadores de Tumor/metabolismo , Carcinogénesis/metabolismo , Citocinas/metabolismo , Neoplasias Mamarias Experimentales , Animales , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Biomarcadores de Tumor/análisis , Biomarcadores de Tumor/sangre , Análisis Químico de la Sangre , Carcinogénesis/efectos de los fármacos , Carcinogénesis/inmunología , Terapia Combinada , Citocinas/análisis , Citocinas/sangre , Monitoreo de Drogas/métodos , Femenino , Linfa/química , Linfa/metabolismo , Neoplasias Mamarias Experimentales/inducido químicamente , Neoplasias Mamarias Experimentales/diagnóstico , Neoplasias Mamarias Experimentales/metabolismo , Neoplasias Mamarias Experimentales/terapia , Mastectomía , Metilnitrosourea , Pronóstico , Ratas , Ratas Wistar , Resultado del TratamientoRESUMEN
We studied the levels microRNA (miR-21, miR-221, miR-222, and miR-429) in blood serum, thoracic duct lymph, and breast cancer tissue, as well as the cell composition of the axillary lymph node in Wistar female rats with chemically induced breast cancer. The levels of miR-221 and miR-429 in the tumor tissue and in the lymph correlated with the decrease in lymphocyte number in the medullary cords of the axillary lymph nodes.
Asunto(s)
Regulación Neoplásica de la Expresión Génica , Ganglios Linfáticos/patología , Glándulas Mamarias Animales/patología , Neoplasias Mamarias Experimentales/genética , MicroARNs/genética , Animales , Axila , Recuento de Células , Femenino , Ganglios Linfáticos/inmunología , Metástasis Linfática , Linfocitos/inmunología , Linfocitos/patología , Macrófagos/inmunología , Macrófagos/patología , Glándulas Mamarias Animales/efectos de los fármacos , Glándulas Mamarias Animales/inmunología , Neoplasias Mamarias Experimentales/inducido químicamente , Neoplasias Mamarias Experimentales/inmunología , Neoplasias Mamarias Experimentales/patología , Metilnitrosourea/administración & dosificación , MicroARNs/inmunología , Ratas , Ratas WistarRESUMEN
We performed a correlation analysis of the morphometric parameters of mesenteric lymph nodes and cytokine content in the lymph of thoracic duct in rats with chemically induced breast cancer. The study showed that activity of the local immune response in the lymph nodes in breast cancer is aimed at antitumor protection. In breast cancer, the area of the paracortical zone remained at the level of the intact group, while the area of lymphoid nodules with germinative centers and the area of medullary substance increased; the number of macrophages in the thymus-dependent zone and zone responsible for humoral immunity also increased. The following positive correlations were revealed: in germinative centers and medullary substance, number of mitotic cells correlated with cytokine IL-5 content and the number of medium lymphocytes correlated with the content of chemokine MIP-1α; in the germinative centers, the number of immunoblasts correlated with the level of cytokine GRO/KC, in the paracortical zone, the number of macrophages correlated with the level of chemokine MCP-1, the number of reticular cells correlated with IL-6 and M-CSF content; in medullary substance, the number of small lymphocytes and mature cells plasma cells (their content was reduced) correlated with the level of chemokine GRO/KC, which can be caused by their migration from the lymph node.
Asunto(s)
Regulación Neoplásica de la Expresión Génica , Ganglios Linfáticos/patología , Glándulas Mamarias Animales/patología , Neoplasias Mamarias Experimentales/genética , Mesenterio/patología , Conducto Torácico/patología , Animales , Quimiocina CCL2/genética , Quimiocina CCL2/inmunología , Quimiocina CCL3/genética , Quimiocina CCL3/inmunología , Quimiocina CXCL1/genética , Quimiocina CXCL1/inmunología , Femenino , Interleucina-5/genética , Interleucina-5/inmunología , Interleucina-6/genética , Interleucina-6/inmunología , Ganglios Linfáticos/inmunología , Metástasis Linfática , Linfocitos/inmunología , Linfocitos/patología , Factor Estimulante de Colonias de Macrófagos/genética , Factor Estimulante de Colonias de Macrófagos/inmunología , Macrófagos/inmunología , Macrófagos/patología , Glándulas Mamarias Animales/efectos de los fármacos , Glándulas Mamarias Animales/inmunología , Neoplasias Mamarias Experimentales/inducido químicamente , Neoplasias Mamarias Experimentales/inmunología , Neoplasias Mamarias Experimentales/patología , Mesenterio/inmunología , Metilnitrosourea/administración & dosificación , Ratas , Ratas Wistar , Conducto Torácico/inmunologíaRESUMEN
We studied the effect of erythropoietin on functional properties of mesenchymal stem cells under conditions of oxidative stress and their therapeutic potential in the treatment of intervertebral disc degeneration in Wistar rats. It was shown that erythropoietin stimulates proliferation under conditions of oxidative stress. Injection of bone marrow mesenchymal stem cells into the damaged intervertebral disc was followed by an increase in the height of the intervertebral disc and activation of repair processes in the nucleus pulposus. The combination of mesenchymal stem cells with erythropoietin provides the best effect of cell therapy in case of intervertebral disc damage.
Asunto(s)
Células de la Médula Ósea/efectos de los fármacos , Eritropoyetina/farmacología , Degeneración del Disco Intervertebral/terapia , Disco Intervertebral/efectos de los fármacos , Trasplante de Células Madre Mesenquimatosas , Células Madre Mesenquimatosas/efectos de los fármacos , Animales , Células de la Médula Ósea/citología , Células de la Médula Ósea/metabolismo , Proliferación Celular/efectos de los fármacos , Modelos Animales de Enfermedad , Fémur/citología , Humanos , Peróxido de Hidrógeno/antagonistas & inhibidores , Peróxido de Hidrógeno/farmacología , Inyecciones Intralesiones , Disco Intervertebral/lesiones , Disco Intervertebral/metabolismo , Degeneración del Disco Intervertebral/metabolismo , Degeneración del Disco Intervertebral/patología , Masculino , Células Madre Mesenquimatosas/citología , Células Madre Mesenquimatosas/metabolismo , Estrés Oxidativo , Ratas , Ratas WistarRESUMEN
We studied the effects of intravenous and lymphotropic administration of bone marrow multipotent mesenchymal stromal cells and products secreted by these cells into conditioned medium on the blood and lymph circulation in the uterus and ovaries, as well as on folliculogenesis in female Wistar rats. It was shown that stromal cells and conditioned media of these cells administered via both routes lead to an increase in the number and diameter of blood vessels in the uterine wall and in the cortical layer of the ovaries. Neither mesenchymal stromal cells, not conditioned media affected the ovarian follicular apparatus.
Asunto(s)
Genitales/patología , Sistema Linfático/patología , Trasplante de Células Madre Mesenquimatosas , Células Madre Multipotentes/citología , Animales , Vasos Sanguíneos/metabolismo , Vasos Sanguíneos/patología , Medios de Cultivo Condicionados , Femenino , Genitales/metabolismo , Sistema Linfático/metabolismo , Vasos Linfáticos/metabolismo , Vasos Linfáticos/patología , Células Madre Multipotentes/metabolismo , Ovario/metabolismo , Ovario/patología , Ratas , Ratas Wistar , Útero/metabolismo , Útero/patologíaRESUMEN
AIM: Ghrelin, a peptide hormone mostly produced by stomach, plays an important role in regulation of feeding behavior, energy balance and glucose homeostasis. THE AIM: to determine the relationships between fasting serum levels of ghrelin, body composition, adipose tissue endocrine function and glucose variability (GV) in type 2 diabetic subjects with and without obesity. MATERIALS AND METHODS: We observed 124 individuals with type 2 diabetes, including 42 non - obese subjects and 82 patients with obesity. Thirty non - obese healthy subjects were acted as control. The concentrations of ghrelin, leptin, resistin, and visfatin in the fasting serum were determined by Multiplex analysis. Body composition was assessed with DEXA. The 24-hour and nocturnal GV parameters were derived from continuous glucose monitoring. RESULTS AND DISCUSSION: Ghrelin levels in patients with diabetes were decreased significantly as compared to control (p.
Asunto(s)
Diabetes Mellitus Tipo 2 , Ghrelina , Tejido Adiposo , Glucemia , Automonitorización de la Glucosa Sanguínea , Glucosa , Humanos , Leptina , ObesidadRESUMEN
The morphometric analysis of mesenteric lymph nodes was carried out in female Wistar rats with chemically induced breast cancer. In control rats with untreated breast cancer, the volume of the system of sinuses increased in parallel with the appearance of morphological signs of suppression of cell-mediated immunity, inhibition of humoral immunity, and macrophage reaction. Against the background of chemotherapy, we observed a decrease in the volume of paracortex and lymphoid nodules, suppression of proliferative activity of lymphoid cells in paracortical and B-cell zone, and a decrease in macrophage content. After resection of breast cancer followed by chemotherapy course, lymph transport activation, widening of the paracortex, enhanced proliferative activity of cells in the paracortex and B-cell zone, and reduced volumes of lymphoid nodules with and without germinal centers and medullary substance were revealed in comparison with rats subjected neoadjuvant chemotherapy.
Asunto(s)
Protocolos de Quimioterapia Combinada Antineoplásica/farmacología , Ganglios Linfáticos/efectos de los fármacos , Subgrupos Linfocitarios/efectos de los fármacos , Neoplasias Mamarias Experimentales/tratamiento farmacológico , Terapia Neoadyuvante/métodos , Animales , Ciclofosfamida/farmacología , Femenino , Fluorouracilo/farmacología , Ganglios Linfáticos/inmunología , Ganglios Linfáticos/patología , Subgrupos Linfocitarios/inmunología , Subgrupos Linfocitarios/patología , Macrófagos/efectos de los fármacos , Macrófagos/inmunología , Macrófagos/patología , Glándulas Mamarias Animales/efectos de los fármacos , Glándulas Mamarias Animales/inmunología , Glándulas Mamarias Animales/patología , Neoplasias Mamarias Experimentales/inducido químicamente , Neoplasias Mamarias Experimentales/inmunología , Neoplasias Mamarias Experimentales/patología , Mastocitos/efectos de los fármacos , Mastocitos/inmunología , Mastocitos/patología , Mesenterio , Metotrexato/farmacología , Metilnitrosourea , Neutrófilos/efectos de los fármacos , Neutrófilos/inmunología , Neutrófilos/patología , Ratas , Ratas WistarRESUMEN
We analyzed cytokine profile in blood serum of patients with uterine myoma and revealed significantly reduced level of IFNγ and a tendency towards a decrease in the levels of IL-1ß and TNFα; the levels of IL-6, IL-8, and IL-12p70 did not differ from those in healthy women. The drop in the concentrations of factors responsible for inflammation and angiogenesis in tissues are unfavorable for proliferation and differentiation of the uterine tissues.
Asunto(s)
Interferón gamma/sangre , Interleucina-1beta/sangre , Leiomioma/sangre , Factor de Necrosis Tumoral alfa/sangre , Neoplasias Uterinas/sangre , Útero/metabolismo , Adulto , Femenino , Expresión Génica , Humanos , Inflamación , Interferón gamma/genética , Interferón gamma/inmunología , Interleucina-12/sangre , Interleucina-12/genética , Interleucina-12/inmunología , Interleucina-1beta/genética , Interleucina-1beta/inmunología , Interleucina-6/sangre , Interleucina-6/genética , Interleucina-6/inmunología , Interleucina-8/sangre , Interleucina-8/genética , Interleucina-8/inmunología , Leiomioma/inmunología , Leiomioma/patología , Leiomioma/cirugía , Persona de Mediana Edad , Factor de Necrosis Tumoral alfa/genética , Factor de Necrosis Tumoral alfa/inmunología , Miomectomía Uterina , Neoplasias Uterinas/inmunología , Neoplasias Uterinas/patología , Neoplasias Uterinas/cirugía , Útero/inmunología , Útero/patología , Útero/cirugíaRESUMEN
The study examined the effects of a novel neurotropic medication based on a lithium complex composed of lithium citrate, polymethylsiloxane, and aluminum oxide on electrophysiological parameters of the rat brain. In contrast to lithium carbonate (the reference drug), the novel preparation resulted in a wave-like dynamics of electrical activity in the visual cortex. Rhythmic photic stimulation of the rats treated with lithium carbonate resulted in appearance of the signs attesting to up-regulation of excitability of cerebral cortex in all examined ranges. In contrast, the complex lithium preparation diminished the delta power spectrum, which was the only affected frequency band. It is hypothesized that the complex lithium medication induces milder activation of the cerebral cortex in comparison with lithium carbonate. The novel medication composed of lithium citrate, aluminum oxide, and polymethylsiloxane, is characterized by greater efficacy and safety than the preparation based on inorganic lithium salt (lithium carbonate).
Asunto(s)
Corteza Cerebral/efectos de los fármacos , Corteza Cerebral/metabolismo , Litio/farmacología , Óxido de Aluminio/farmacología , Animales , Encéfalo/efectos de los fármacos , Encéfalo/metabolismo , Citratos/farmacología , Fenómenos Electrofisiológicos/efectos de los fármacos , Litio/química , Carbonato de Litio/farmacología , Masculino , Ratas , Siliconas/farmacologíaRESUMEN
The study examined the effect of bone marrow multipotent mesenchymal stromal cells and their products secreted into the conditioned medium on uterine microcirculatory bed in Wistar rats during chronic inflammation. The parameters of blood and lymphatic microcirculation in the uterus changed in various directions in relation to the administration routes of biomedical cell product, which is important when the cell therapy is employed in the treatment of inflammatory-degenerative alterations in the lesser pelvis organs.
Asunto(s)
Medios de Cultivo Condicionados/farmacología , Endometritis/patología , Vasos Linfáticos , Células Madre Mesenquimatosas/fisiología , Microcirculación , Útero , Animales , Células de la Médula Ósea/fisiología , Células Cultivadas , Enfermedad Crónica , Medios de Cultivo Condicionados/metabolismo , Modelos Animales de Enfermedad , Endometritis/terapia , Femenino , Vasos Linfáticos/efectos de los fármacos , Vasos Linfáticos/fisiología , Trasplante de Células Madre Mesenquimatosas , Células Madre Mesenquimatosas/metabolismo , Microcirculación/efectos de los fármacos , Ratas , Ratas Wistar , Útero/irrigación sanguínea , Útero/efectos de los fármacos , Útero/patologíaRESUMEN
Existence of lymphatic outflow of intraocular fluid is still an open question. Identification of the lymphatic capillaries and vessels in various human organs in normal and pathological conditions became possible with discovery of endotheliocyte markers for lymphatic vessels. However, the available information on the presence of lymphatic structures in the human eye is inconsistent and uncertain. The data on lymphatic drainage of the eye is of interest, particularly because it may help understand glaucoma pathogenesis, mechanisms of development of eye inflammatory diseases, and develop pathogenetic therapies. The article reviews literature and presents the authors' own views on lymphatic drainage of the human eye.