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1.
Curr Biol ; 30(24): 4921-4931.e5, 2020 12 21.
Artículo en Inglés | MEDLINE | ID: mdl-33065015

RESUMEN

Retinal rod and cone photoreceptors mediate vision in dim and bright light, respectively, by transducing absorbed photons into neural electrical signals. Their phototransduction mechanisms are essentially identical. However, one difference is that, whereas a rod visual pigment remains stable in darkness, a cone pigment has some tendency to dissociate spontaneously into apo-opsin and retinal (the chromophore) without isomerization. This cone-pigment property is long known but has mostly been overlooked. Importantly, because apo-opsin has weak constitutive activity, it triggers transduction to produce electrical noise even in darkness. Currently, the precise dark apo-opsin contents across cone subtypes are mostly unknown, as are their dark activities. We report here a study of goldfish red (L), green (M), and blue (S) cones, finding with microspectrophotometry widely different apo-opsin percentages in darkness, being ∼30% in L cones, ∼3% in M cones, and negligible in S cones. L and M cones also had higher dark apo-opsin noise than holo-pigment thermal isomerization activity. As such, given the most likely low signal amplification at the pigment-to-transducin/phosphodiesterase phototransduction step, especially in L cones, apo-opsin noise may not be easily distinguishable from light responses and thus may affect cone vision near threshold.


Asunto(s)
Oscuridad , Fototransducción/fisiología , Opsinas/metabolismo , Células Fotorreceptoras Retinianas Conos/fisiología , Animales , Carpa Dorada , Modelos Animales , Técnicas de Placa-Clamp , Estimulación Luminosa/métodos , Células Fotorreceptoras Retinianas Conos/efectos de la radiación , Análisis de la Célula Individual
2.
Photochem Photobiol Sci ; 19(10): 1300-1307, 2020 Oct 14.
Artículo en Inglés | MEDLINE | ID: mdl-32812970

RESUMEN

Retinal, the vitamin A aldehyde, is a potent photosensitizer that plays a major role in light-induced damage to vertebrate photoreceptors. 11-Cis retinal is the light-sensitive chromophore of rhodopsin, the photopigment of vertebrate rod photoreceptors. It is isomerized by light to all-trans, activating rhodopsin and beginning the process of light detection. All-trans retinal is released by activated rhodopsin, allowing its regeneration by fresh 11-cis retinal continually supplied to photoreceptors. The released all-trans retinal is reduced to all-trans retinol in a reaction using NADPH. We have examined the photooxidation mediated by 11-cis and all-trans retinal in single living rod photoreceptors isolated from mouse retinas. Photooxidation was measured with fluorescence imaging from the oxidation of internalized BODIPY C11, a fluorescent dye whose fluorescence changes upon oxidation. We found that photooxidation increased with the concentration of exogenously added 11-cis or all-trans retinal to metabolically compromised rod outer segments that lacked NADPH supply. In dark-adapted metabolically intact rod outer segments with access to NADPH, there was no significant increase in photooxidation following exposure of the cell to light, but there was significant increase following addition of exogenous 11-cis retinal. The results indicate that both 11-cis and all-trans retinal can mediate light-induced damage in rod photoreceptors. In metabolically intact cells, the removal of the all-trans retinal generated by light through its reduction to retinol minimizes all-trans retinal-mediated photooxidation. However, because the enzymatic machinery of the rod outer segment cannot remove 11-cis retinal, 11-cis-retinal-mediated photooxidation may play a significant role in light-induced damage to photoreceptor cells.


Asunto(s)
Células Fotorreceptoras/química , Retinaldehído/química , Segmento Externo de la Célula en Bastón/química , Vitamina A/química , Animales , Ratones , Ratones Noqueados , Estructura Molecular , Imagen Óptica , Oxidación-Reducción , Procesos Fotoquímicos
3.
Trans Am Ophthalmol Soc ; 115: T1, 2017 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-28900371

RESUMEN

PURPOSE: To test the hypothesis that delayed dark adaptation in patients with macular degeneration is due to an excess of free unliganded opsin (apo-opsin) and a deficiency of the visual chromophore, 11-cis retinal, in rod outer segments. METHODS: A total of 50 human autopsy eyes were harvested from donors with and without macular degeneration within 2-24 hrs. postmortem. Protocols were developed which permitted dark adaptation of normal human eyes after death and enucleation. Biochemical methods of purifying rod outer segments were optimized and the concentration of rhodopsin and apo-opsin was measured with UV-visible scanning spectroscopy. The presence of apo-opsin was calculated by measuring the difference in the rhodopsin absorption spectra before and after the addition of 11-cis retinal. RESULTS: A total of 20 normal eyes and 16 eyes from donors with early, intermediate and advanced stages of macular degeneration were included in the final analysis. Dark adaptation was achieved by harvesting whole globes in low light, transferring into dark (light-proof) canisters and dissecting the globes using infrared light and image converters for visualization. Apo-opsin was readily detected in positive controls after the addition of 11-cis retinal. Normal autopsy eyes showed no evidence of apo-opsin. Eyes with macular degeneration also showed no evidence of apo-opsin, regardless of the severity of disease. CONCLUSIONS: Methods have been developed to study dark adaptation in human autopsy eyes. Eyes with age-related macular degeneration do not show a deficiency of 11-cis retinal or an excess of apo-opsin within rod outer segments.


Asunto(s)
Consenso , Adaptación a la Oscuridad/fisiología , Degeneración Macular/fisiopatología , Oftalmología , Opsinas/metabolismo , Retinaldehído/deficiencia , Sociedades Médicas , Humanos , Degeneración Macular/metabolismo , Segmento Externo de la Célula en Bastón/metabolismo
4.
Prog Mol Biol Transl Sci ; 134: 449-63, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26310170

RESUMEN

Lipofuscin is highly fluorescent material, formed in several tissues but best studied in the eye. The accumulation of lipofuscin in the retinal pigment epithelium (RPE) is a hallmark of aging in the eye and has been implicated in various retinal degenerations, including age-related macular degeneration. The bis-retinoid N-retinyl-N-retinylidene ethanolamine (A2E), formed from retinal, has been identified as a byproduct of the visual cycle, and numerous in vitro studies have found toxicity associated with this compound. The compound is known to accumulate in the RPE with age and was the first identified compound extracted from lipofuscin. Our studies have correlated the distribution of lipofuscin and A2E across the human and mouse RPE. Lipofuscin fluorescence was imaged in the RPE from human donors of various ages and from assorted mouse models. The spatial distribution of A2E was determined using matrix-assisted laser desorption-ionization imaging mass spectrometry on both flat-mounted and transversally sectioned RPE tissue. Our data support the clinical observations in humans of strong RPE fluorescence, increasing with age, in the central area of the RPE. However, there was no correlation between the distribution of A2E and lipofuscin, as the levels of A2E were highest in the far periphery and decreased toward the central region. Interestingly, in all the mouse models, A2E distribution and lipofuscin fluorescence correlate well. These data demonstrate that the accumulation of A2E is not responsible for the increase in lipofuscin fluorescence observed in the central RPE with aging in humans.


Asunto(s)
Lipofuscina/metabolismo , Retinoides/metabolismo , Animales , Humanos , Imagenología Tridimensional , Modelos Biológicos , Retinoides/química , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
5.
Prog Mol Biol Transl Sci ; 134: 465-76, 2015.
Artículo en Inglés | MEDLINE | ID: mdl-26310171

RESUMEN

Cones are photoreceptor cells used for bright light and color vision. Retinoids are vitamin A derivatives, one of which is the 11-cis aldehyde form that serves as the chromophore for both cone and rod visual pigments. In the visual disease, Type 2 Leber congenital amaurosis (LCA2), 11-cis-retinal generation is inhibited or abolished. Work by others has shown that patients with LCA2 have symptoms consistent with degenerating cones. In mouse models for LCA2, early cone degeneration is readily apparent: cone opsins and other proteins associated with the outer segment are delocalized and cell numbers decline rapidly within the first month. Rods would appear normal morphologically and functionally, if not for the absence of chromophore. Supplementation of mouse models of LCA2 with cis-retinoids has been shown to slow loss of cone photoreceptor cells if mice were maintained in darkness. Thus, 11-cis-retinal appears not only to have a role in the light response reaction but also to promote proper trafficking of the cone opsins and maintain viable cones.


Asunto(s)
Células Fotorreceptoras Retinianas Conos/metabolismo , Retinoides/fisiología , Animales , Modelos Animales de Enfermedad , Humanos , Amaurosis Congénita de Leber/patología , Opsinas/metabolismo , Vías Visuales/metabolismo
6.
J Neurosci ; 34(40): 13336-48, 2014 Oct 01.
Artículo en Inglés | MEDLINE | ID: mdl-25274813

RESUMEN

Retinitis pigmentosa (RP) is an inherited neurodegenerative disease involving progressive vision loss, and is often linked to mutations in the rhodopsin gene. Mutations that abolish N-terminal glycosylation of rhodopsin (T4K and T17M) cause sector RP in which the inferior retina preferentially degenerates, possibly due to greater light exposure of this region. Transgenic animal models expressing rhodopsin glycosylation mutants also exhibit light exacerbated retinal degeneration (RD). In this study, we used transgenic Xenopus laevis to investigate the pathogenic mechanism connecting light exposure and RD in photoreceptors expressing T4K or T17M rhodopsin. We demonstrate that increasing the thermal stability of these rhodopsins via a novel disulfide bond resulted in significantly less RD. Furthermore, T4K or T17M rhodopsins that were constitutively inactive (due to lack of the chromophore-binding site or dietary deprivation of the chromophore precursor vitamin A) induced less toxicity. In contrast, variants in the active conformation accumulated in the ER and caused RD even in the absence of light. In vitro, T4K and T17M rhodopsins showed reduced ability to regenerate pigment after light exposure. Finally, although multiple amino acid substitutions of T4 abolished glycosylation at N2 but were not toxic, similar substitutions of T17 were not tolerated, suggesting that the carbohydrate moiety at N15 is critical for cell viability. Our results identify a novel pathogenic mechanism in which the glycosylation-deficient rhodopsins are destabilized by light activation. These results have important implications for proposed RP therapies, such as vitamin A supplementation, which may be ineffective or even detrimental for certain RP genotypes.


Asunto(s)
Luz , Mutación/genética , Degeneración Retiniana/etiología , Retinitis Pigmentosa , Rodopsina/genética , Segmento Externo de la Célula en Bastón/patología , Análisis de Varianza , Animales , Animales Modificados Genéticamente , Células COS , Chlorocebus aethiops , Modelos Animales de Enfermedad , Humanos , Microscopía Confocal , Degeneración Retiniana/dietoterapia , Retinitis Pigmentosa/complicaciones , Retinitis Pigmentosa/genética , Retinitis Pigmentosa/patología , Estadísticas no Paramétricas , Transfección , Vitamina A/administración & dosificación , Vitamina A/metabolismo , Aglutininas del Germen de Trigo/metabolismo , Xenopus laevis
7.
Chem Biol ; 21(3): 309-10, 2014 Mar 20.
Artículo en Inglés | MEDLINE | ID: mdl-24655920

RESUMEN

In this issue of Chemistry & Biology, Srinivasan and colleagues describe their study of ligand-protein interactions in visual pigments. Comparing the more stable isomeric ligand 9-cis retinal to the physiologically occurring 11-cis retinal, they report differences in ligand specificity and opsin conformational stability not previously described for bleached rhodopsin.


Asunto(s)
Receptores Acoplados a Proteínas G/metabolismo , Pigmentos Retinianos/metabolismo , Retinaldehído/metabolismo , Rodopsina/metabolismo , Animales , Humanos
8.
J Comp Neurol ; 522(10): 2249-65, 2014 Jul 01.
Artículo en Inglés | MEDLINE | ID: mdl-24374736

RESUMEN

Although more than one type of visual opsin is present in the retina of most vertebrates, it was thought that each type of photoreceptor expresses only one opsin. However, evidence has accumulated that some photoreceptors contain more than one opsin, in many cases as a result of a developmental transition from the expression of one opsin to another. The salamander UV-sensitive (UV) cone is particularly notable because it contains three opsins (Makino and Dodd [1996] J Gen Physiol 108:27-34). Two opsin types are expressed at levels more than 100 times lower than the level of the primary opsin. Here, immunohistochemical experiments identified the primary component as a UV cone opsin and the two minor components as the short wavelength-sensitive (S) and long wavelength-sensitive (L) cone opsins. Based on single-cell recordings of 156 photoreceptors, the presence of three components in UV cones of hatchlings and terrestrial adults ruled out a developmental transition. There was no evidence for multiple opsin types within rods or S cones, but immunohistochemistry and partial bleaching in conjunction with single-cell recording revealed that both single and double L cones contained low levels of short wavelength-sensitive pigments in addition to the main L visual pigment. These results raise the possibility that coexpression of multiple opsins in other vertebrates was overlooked because a minor component absorbing at short wavelengths was masked by the main visual pigment or because the expression level of a component absorbing at long wavelengths was exceedingly low.


Asunto(s)
Ambystoma/crecimiento & desarrollo , Ambystoma/fisiología , Opsinas/metabolismo , Células Fotorreceptoras Retinianas Conos/fisiología , Ambystoma/anatomía & histología , Animales , Inmunohistoquímica , Microelectrodos , Estimulación Luminosa , Células Fotorreceptoras Retinianas Conos/citología , Pigmentos Retinianos , Células Fotorreceptoras Retinianas Bastones/citología , Células Fotorreceptoras Retinianas Bastones/fisiología , Rayos Ultravioleta , Visión Ocular/fisiología
9.
Mol Vis ; 19: 1149-57, 2013.
Artículo en Inglés | MEDLINE | ID: mdl-23734084

RESUMEN

PURPOSE: In the absence of 11-cis retinal (e.g., Rpe65⁻/⁻), the chromophore for all pigments, cone opsins are mislocalized in vivo. Using the systemic application of 11-cis retinal, appropriate protein localization can be promoted. Here, we asked whether explant cultures of Rpe65⁻/⁻ mouse retina are amenable to screening retinoids for their ability to promote opsin trafficking. METHODS: Retina-retinal pigment epithelium (RPE) cultures were prepared from 7-day-old Rpe65⁻/⁻ Rho⁻/⁻ or wild-type pups and cultured for 11 days. Explants were treated with retinoids throughout this period. Ultraviolet (UV)-opsin trafficking was analyzed by immunohistochemistry and quantitative image analysis, while its messenger RNA expression was examined by quantitative real-time PCR, and the interaction of retinoids with UV-opsin was probed in transducing-activation assays. RESULTS: In wild-type explant cultures, UV-opsin was restricted to the outer segments, whereas in those derived from Rpe65⁻/⁻ Rho⁻/⁻ mice, opsin trafficking was impaired. In Rpe65⁻/⁻ Rho⁻/⁻ explants, administration of 11-cis retinal, 11-cis retinol or retinoic acid (RA) reversed the opsin trafficking phenotype. RA analogs designed to act by binding to the retinoic acid receptor or the retinoid X-receptor, however, had no effect. RA was shown to interact with the UV-cone opsin, demonstrated by its ability to effect ligand-dependent activation of transducin by UV-cone opsin. All compounds tested increased cone opsin messenger RNA expression. CONCLUSIONS: Cone-opsin trafficking defects were replicated in Rpe65⁻/⁻ Rho⁻/⁻ retina-RPE cultures, and were reversed by 11-cis retinal treatment. Comparing the effects of different retinoids on their ability to promote UV-opsin trafficking to outer segments confirmed the critical role of agents that bind in the retinoid binding pocket. Retinoids that act as transcription factors, however, were ineffective. Thus, organ cultures may be a powerful low-throughput screening tool to identify novel compounds to promote cone survival.


Asunto(s)
Opsinas de los Conos/metabolismo , Modelos Biológicos , Técnicas de Cultivo de Órganos/métodos , Retina/metabolismo , cis-trans-Isomerasas/deficiencia , Animales , Ratones , Ratones Endogámicos C57BL , Peso Molecular , Especificidad de Órganos/efectos de los fármacos , Especificidad de Órganos/efectos de la radiación , Fenotipo , Transporte de Proteínas/efectos de los fármacos , Transporte de Proteínas/efectos de la radiación , Retina/efectos de los fármacos , Retina/efectos de la radiación , Epitelio Pigmentado de la Retina/efectos de los fármacos , Epitelio Pigmentado de la Retina/metabolismo , Epitelio Pigmentado de la Retina/efectos de la radiación , Retinaldehído/farmacología , Rodopsina/deficiencia , Rodopsina/metabolismo , Tretinoina/farmacología , Rayos Ultravioleta , Vitamina A/farmacología , cis-trans-Isomerasas/metabolismo
10.
Prog Retin Eye Res ; 32: 48-63, 2013 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-23063666

RESUMEN

The retinoid cycle is a series of biochemical reactions within the eye that is responsible for synthesizing the chromophore, 11-cis retinal, for visual function. The chromophore is bound to G-protein coupled receptors, opsins, within rod and cone photoreceptor cells forming the photosensitive visual pigments. Integral to the sustained function of photoreceptors is the continuous generation of chromophore by the retinoid cycle through two separate processes, one that supplies both rods and cones and another that exclusively supplies cones. Recent findings such as RPE65 localization within cones and the pattern of distribution of retinoid metabolites within mouse and human retinas have challenged previous proposed schemes. This review will focus on recent findings regarding the transport of retinoids, the mechanisms by which chromophore is supplied to both rods and cones, and the metabolism of retinoids within the posterior segment of the eye.


Asunto(s)
Proteínas del Ojo/metabolismo , Retina/metabolismo , Células Fotorreceptoras Retinianas Conos/metabolismo , Pigmentos Retinianos/metabolismo , Retinoides/metabolismo , Animales , Humanos
11.
J Nat Prod ; 74(3): 391-4, 2011 Mar 25.
Artículo en Inglés | MEDLINE | ID: mdl-21314100

RESUMEN

Retinal analogues have been used to probe the chromophore binding pocket and function of the rod visual pigment rhodopsin. Despite the high homology between rod and cone visual pigment proteins, conclusions drawn from rhodopsin studies should not necessarily be extrapolated to cone visual pigment proteins. In this study, the effects of full-length and truncated retinal analogues on the human red cone opsin's ability to activate transducin, the G protein in visual transduction, were assessed. The result with beta-ionone (6) confirms that a covalent bond is not necessary to deactivate the red cone opsin. In addition, several small compounds were found able to deactivate this opsin. However, as the polyene chain is extended in a trans configuration beyond the 9-carbon position, the analogues became agonists up to all-trans-retinal (3). The 22-carbon analogue (2) appeared to be neither an agonist nor an inverse agonist. Although the all-trans-C17 (5) analogue was an agonist, the 9-cis-C17 (11) compound was an inverse agonist, a result that differs from that with rhodopsin. These results suggest that the red cone opsin has a more open structure in the chromophore binding region than rhodopsin and its activation or deactivation as a G-protein receptor may be less selective than rhodopsin.


Asunto(s)
Opsinas de los Conos/metabolismo , Retinaldehído , Animales , Bovinos , Opsinas de los Conos/química , Humanos , Estructura Molecular , Retina/química , Pigmentos Retinianos/metabolismo , Retinaldehído/análogos & derivados , Retinaldehído/química , Retinaldehído/metabolismo , Rodopsina/metabolismo , Estereoisomerismo , Transducina/metabolismo
12.
Invest Ophthalmol Vis Sci ; 52(5): 2412-6, 2011 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-21228385

RESUMEN

PURPOSE: To determine the effect of light/dark cycles on the cones of 11-cis retinal-treated RPE65/rhodopsin double knockout (Rpe65(-/-)Rho(-/-)) mice. Studies have shown that cones degenerate in chromophore-deficient mouse models for Leber Congenital Amaurosis (LCA), but exogenous supplementation of the native 11-cis retinal chromophore can inhibit this degeneration, suggesting that 11-cis retinal could be used as a therapeutic agent for preserving functional cones in patients with LCA. However, these treated mice were maintained in the dark. METHODS: 11-cis Retinal was introduced into Rpe65(-/-)Rho(-/-) mice at postnatal day 10 as a single subcutaneous injection mixed with a basement membrane matrix. The mice were maintained in either normal light/dark cycles or constant dark conditions. Fluorescence microscopy was used to assess retinal morphology. Cone cell survival was determined by counting cone opsin-containing cells on flat-mounted P30 retinas. Cross-sections of P21 mouse retina were used to assess cone cell integrity by visualizing opsin localization. Cone function was determined by electroretinography (ERG). RESULTS: Previous studies have shown that 11-cis retinal-treated mice lacking RPE65 and raised in constant dark have higher cone photoreceptor cell number, improved cone opsin localization, and enhanced cone ERG signals when compared with untreated mice. However, in this study the authors show that 11-cis retinal-treated Rpe65(-/-)Rho(-/-) mice raised in cyclic light did not show the improvements seen with the dark-reared mice. CONCLUSIONS: Thus, 11-cis retinal by itself, as well as other agents that form photosensitive pigments, will not be good therapeutic candidates for preserving cones in LCA.


Asunto(s)
Proteínas Portadoras/genética , Modelos Animales de Enfermedad , Proteínas del Ojo/genética , Amaurosis Congénita de Leber/tratamiento farmacológico , Luz , Células Fotorreceptoras Retinianas Conos/patología , Retinaldehído/uso terapéutico , Rodopsina/genética , Animales , Recuento de Células , Supervivencia Celular , Adaptación a la Oscuridad , Electrorretinografía , Técnicas de Inactivación de Genes , Amaurosis Congénita de Leber/genética , Amaurosis Congénita de Leber/metabolismo , Ratones , Ratones Noqueados , Microscopía Fluorescente , Opsinas/metabolismo , Células Fotorreceptoras Retinianas Conos/efectos de la radiación , cis-trans-Isomerasas
13.
Methods Enzymol ; 485: 213-24, 2010.
Artículo en Inglés | MEDLINE | ID: mdl-21050919

RESUMEN

Visual pigment proteins belong to the superfamily of G protein-coupled receptors and are the light-sensitive molecules in rod and cone photoreceptor cells. The protein moiety is known as opsin and the ligand in the dark is 11-cis retinal, which serves as both the photon detector and an inverse agonist. While much is known about properties of the rod pigment rhodopsin, much less is understood about cone visual pigments. Being able to identify ligands that effect opsins give an insight into structure-activity relationships. The action of some ligands indicates that there are differences between not only rod and cone opsins but also among the different classes of cone opsins. Furthermore, inverse agonists of cone opsins may have potential therapeutic uses under conditions when the native 11-cis retinal ligand is absent. A method for determining the effects of ligands on rod and cone opsin activity is described.


Asunto(s)
Evaluación Preclínica de Medicamentos/métodos , Agonismo Inverso de Drogas , Opsinas/metabolismo , Transducina/metabolismo , Animales , Humanos , Ligandos
14.
Methods Mol Biol ; 652: 85-94, 2010.
Artículo en Inglés | MEDLINE | ID: mdl-20552423

RESUMEN

Upon absorption of a photon, the bound 11-cis-retinoid isomerizes to the all-trans form resulting in a protein conformational change that enables it to activate its G protein, transducin, to begin the visual signal transduction cascade. The native ligand, 11-cis-retinal, acts as an inverse agonist to both the apoproteins of rod and cone visual pigments (opsins); all-trans-retinal is an agonist. Truncated analogs of retinal have been used to characterize structure-function relationships with rod opsins, but little has been done with cone opsins. Activation of transducin by an opsin is one method to characterize the conformational state of the opsin. This chapter describes an in vitro transducin activation assay that can be used with cone opsins to determine the degree to which different ligands can act as an agonist or an inverse agonist to gain insight into the ligand-binding pocket of cone opsins and differences between the different classes of opsins. The understanding of the effects of ligands on cone opsin activity can potentially be applied to future therapeutic agents targeting opsins.


Asunto(s)
Opsinas de los Conos/agonistas , Opsinas de los Conos/metabolismo , Agonismo Inverso de Drogas , Retinaldehído/análogos & derivados , Retinaldehído/farmacología , Opsinas de Bastones/agonistas , Opsinas de Bastones/metabolismo , Animales , Células COS , Bovinos , Membrana Celular/metabolismo , Chlorocebus aethiops , Opsinas de los Conos/química , Humanos , Opsinas de Bastones/química , Transducina/metabolismo
15.
Biochemistry ; 48(20): 4294-304, 2009 May 26.
Artículo en Inglés | MEDLINE | ID: mdl-19348429

RESUMEN

Rhodopsin is palmitylated at two cysteine residues in its carboxyl terminal region. We have looked at the effects of palmitylation on the molecular interactions formed by rhodopsin using single-molecule force spectroscopy and the function of rhodopsin using both in vitro and in vivo approaches. A knockin mouse model expressing palmitate-deficient rhodopsin was used for live animal in vivo studies and to obtain native tissue samples for in vitro assays. We specifically looked at the effects of palmitylation on the chromophore-binding pocket, interactions of rhodopsin with transducin, and molecular interactions stabilizing the receptor structure. The structure of rhodopsin is largely unperturbed by the absence of palmitate linkage. The binding pocket for the chromophore 11-cis-retinal is minimally altered as palmitate-deficient rhodopsin exhibited the same absorbance spectrum as wild-type rhodopsin. Similarly, the rate of release of all-trans-retinal after light activation was the same both in the presence and absence of palmitylation. Significant differences were observed in the rate of transducin activation by rhodopsin and in the force required to unfold the last stable structural segment in rhodopsin at its carboxyl terminal end. A 1.3-fold reduction in the rate of transducin activation by rhodopsin was observed in the absence of palmitylation. Single-molecule force spectroscopy revealed a 2.1-fold reduction in the normalized force required to unfold the carboxyl terminal end of rhodopsin. The absence of palmitylation in rhodopsin therefore destabilizes the molecular interactions formed in the carboxyl terminal end of the receptor, which appears to hinder the activation of transducin by light-activated rhodopsin.


Asunto(s)
Ácido Palmítico/química , Rodopsina/química , Rodopsina/fisiología , Animales , Células COS , Chlorocebus aethiops , Cisteína/química , Luz , Ratones , Ratones Endogámicos C57BL , Modelos Moleculares , Conformación Molecular , Unión Proteica , Estructura Terciaria de Proteína , Segmento Externo de la Célula en Bastón/metabolismo , Transducina/química
16.
J Biol Chem ; 284(24): 16492-16500, 2009 Jun 12.
Artículo en Inglés | MEDLINE | ID: mdl-19386593

RESUMEN

11-cis-retinol has previously been shown in physiological experiments to promote dark adaptation and recovery of photoresponsiveness of bleached salamander red cones but not of bleached salamander red rods. The purpose of this study was to evaluate the direct interaction of 11-cis-retinol with expressed human and salamander cone opsins, and to determine by microspectrophotometry pigment formation in isolated salamander photoreceptors. We show here in a cell-free system using incorporation of radioactive guanosine 5'-3-O-(thio)triphosphate into transducin as an index of activity, that 11-cis-retinol inactivates expressed salamander cone opsins, acting an inverse agonist. Similar results were obtained with expressed human red and green opsins. 11-cis-retinol had no significant effect on the activity of human blue cone opsin. In contrast, 11-cis-retinol activates the expressed salamander and human red rod opsins, acting as an agonist. Using microspectrophotometry of salamander cone photoreceptors before and after bleaching and following subsequent treatment with 11-cis-retinol, we show that 11-cis-retinol promotes pigment formation. Pigment was not formed in salamander red rods or green rods (containing the same opsin as blue cones) treated under the same conditions. These results demonstrate that 11-cis-retinol is not a useful substrate for rod photoreceptors although it is for cone photoreceptors. These data support the premise that rods and cones have mechanisms for handling retinoids and regenerating visual pigment that are specific to photoreceptor type. These mechanisms are critical to providing regenerated pigments in a time scale required for the function of these two types of photoreceptors.


Asunto(s)
Opsinas de los Conos/agonistas , Opsinas de los Conos/metabolismo , Células Fotorreceptoras Retinianas Conos/efectos de los fármacos , Células Fotorreceptoras Retinianas Conos/metabolismo , Vitamina A/farmacología , Vitaminas/farmacología , Ambystoma , Animales , Sistema Libre de Células , Adaptación a la Oscuridad/efectos de los fármacos , Adaptación a la Oscuridad/fisiología , Agonismo Inverso de Drogas , Humanos , Epitelio Pigmentado de la Retina/metabolismo , Células Fotorreceptoras Retinianas Bastones/efectos de los fármacos , Células Fotorreceptoras Retinianas Bastones/metabolismo , Opsinas de Bastones/metabolismo
17.
Biochemistry ; 47(28): 7567-71, 2008 Jul 15.
Artículo en Inglés | MEDLINE | ID: mdl-18563917

RESUMEN

Rhodopsin is the photosensitive pigment in the rod photoreceptor cell. Upon absorption of a photon, the covalently bound 11- cis-retinal isomerizes to the all- trans form, enabling rhodopsin to activate transducin, its G protein. All -trans-retinal is then released from the protein and reduced to all -trans-retinol. It is subsequently transported to the retinal pigment epithelium where it is converted to 11- cis-retinol and oxidized to 11- cis-retinal before it is transported back to the photoreceptor to regenerate rhodopsin and complete the visual cycle. In this study, we have measured the effects of all -trans- and 11- cis-retinals and -retinols on the opsin's ability to activate transducin to ascertain their potentials for activating the signaling cascade. Only 11- cis-retinal acts as an inverse agonist to the opsin. All -trans-retinal, all -trans-retinol, and 11- cis-retinol are all agonists with all -trans-retinal being the most potent agonist and all -trans-retinol being the least potent. Taken as a whole, our study is consistent with the hypothesis that the steps in the visual cycle are optimized such that the rod can serve as a highly sensitive dim light receptor. All -trans-retinal is immediately reduced in the photoreceptor to prevent back reactions and to weaken its effectiveness as an agonist before it is transported out of the cell; oxidation of 11- cis-retinol occurs in the retinal pigment epithelium and not the rod photoreceptor cell because 11- cis-retinol can act as an agonist and activate the signaling cascade if it were to bind an opsin, effectively adapting the cell to light.


Asunto(s)
Células Fotorreceptoras Retinianas Bastones/fisiología , Retinaldehído/farmacología , Rodopsina/metabolismo , Transducina/fisiología , Visión Ocular/fisiología , Vitamina A/farmacología , Animales , Células COS , Bovinos , Chlorocebus aethiops , Haplorrinos , Células Fotorreceptoras/fisiología , Retinaldehído/química , Retinaldehído/metabolismo , Vitamina A/química
18.
Biochemistry ; 46(43): 12248-52, 2007 Oct 30.
Artículo en Inglés | MEDLINE | ID: mdl-17918963

RESUMEN

The crystal structures of rhodopsin depict the inactive conformation of rhodopsin in the dark. The 11-cis retinoid chromophore, the inverse agonist holding rhodopsin inactive, is well-resolved. Thr118 in helix 3 is the closest amino acid residue next to the 9-methyl group of the chromophore. The 9-methyl group of retinal facilitates the transition from an inactive metarhodopsin I to the active metarhodopsin II intermediate. In this study, a site-specific mutation of Thr118 to the bulkier Trp was made with the idea to induce an active conformation of the protein. The data indicate that such a mutation does indeed result in an active protein that depends on the presence of the ligand, specifically the 9-methyl group. As a result of this mutation, 11-cis retinal has been converted to an agonist. The apoprotein form of this mutant is no more active than the wild-type apoprotein. However, unlike wild-type rhodopsin, the covalent linkage of the ligand can be attacked by hydroxylamine in the dark. The combination of the Thr118Trp mutation and the 9-methyl group of the chromophore behaves as a "steric doorstop" holding the protein in an open and active conformation.


Asunto(s)
Ingeniería de Proteínas , Rodopsina/química , Animales , Células COS , Bovinos , Chlorocebus aethiops , Modelos Moleculares , Mutación , Rodopsina/agonistas , Rodopsina/antagonistas & inhibidores , Rodopsina/genética , Rodopsina/aislamiento & purificación , Espectrofotometría Ultravioleta
19.
Vision Res ; 46(27): 4493-501, 2006 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-16989884

RESUMEN

Palmitylation is a widespread modification in G-protein-coupled receptors and often a dynamic process. In rhodopsins, palmitylation is static on C322/C323. Red/green (M/LWS) cone opsins have no cysteines at corresponding positions and no palmitylation. Blue (SWS2) cone opsins have a single corresponding cysteine and mass spectrometric analysis showed partial palmitylation of salamander SWS2 cone opsin. Ultraviolet (SWS1) cone opsins have one corresponding cysteine, but only unpalmitylated opsin was observed for mouse and salamander. The results show that the static palmitylation found on rhodopsin is not found on cone opsins and suggest the possibility of an unidentified role for opsin palmitylation in cones.


Asunto(s)
Ácido Palmítico/metabolismo , Células Fotorreceptoras Retinianas Conos/metabolismo , Opsinas de Bastones/metabolismo , Animales , Secuencia de Bases , Células COS , Bovinos , Chlorocebus aethiops , Cromatografía por Intercambio Iónico , Lagartos , Ratones , Datos de Secuencia Molecular , Proteínas Recombinantes/análisis , Proteínas Recombinantes/genética , Rodopsina/genética , Rodopsina/metabolismo , Opsinas de Bastones/genética , Alineación de Secuencia , Análisis de Secuencia de ADN , Análisis Espectral , Urodelos
20.
Photochem Photobiol ; 82(6): 1442-6, 2006.
Artículo en Inglés | MEDLINE | ID: mdl-16906791

RESUMEN

The 9-methyl group of 11-cis retinal is important in the efficient formation of the active conformation of rhodopsin, Meta II. Here, Tyrl91 rhodopsin mutants were generated because of its proximity to that methyl group in the dark structure. If photoactivation results in movement of the 9-methyl group toward Tyrl91, the steric interactions involved with activation and/or deactivation might not be as tightly coupled in mutant proteins with smaller amino acids at that position. Tyrl91 mutations have no effect on the dark pigment. However, after photobleaching, the lifetime of Meta II is shorter; Meta II decays quickly into two inactive species: (1) a Meta III or Meta III-like species and (2) opsin and free retinal. The Meta III-like fraction maintains the covalent Schiff base linkage of the chromophore much longer than the wild type. On the other hand, the fast chromophore release is similar to cone pigments. Taken together, the data suggest that the role of the 9-methyl group after photo-isomerization is not only to form Meta II efficiently, but also to maintain its active conformation and allow for the timely hydrolysis of the Schiff base. Perturbation of this interaction effects changes in the hydrolysis of the Schiff base and for the case of the Y191A mutation the folded structure of the protein after photobleaching.


Asunto(s)
Mutación , Rodopsina/química , Rodopsina/genética , Sustitución de Aminoácidos , Animales , Células COS , Chlorocebus aethiops , Secuencias Hélice-Asa-Hélice , Cinética , Modelos Moleculares , Polimorfismo de Nucleótido Simple , Proteínas Recombinantes/química , Espectrofotometría
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